CN216365007U - Line field confocal OCT device - Google Patents
Line field confocal OCT device Download PDFInfo
- Publication number
- CN216365007U CN216365007U CN202122247018.XU CN202122247018U CN216365007U CN 216365007 U CN216365007 U CN 216365007U CN 202122247018 U CN202122247018 U CN 202122247018U CN 216365007 U CN216365007 U CN 216365007U
- Authority
- CN
- China
- Prior art keywords
- lens
- module
- light
- sample
- beam splitting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn - After Issue
Links
- 230000007246 mechanism Effects 0.000 claims abstract description 26
- 238000006073 displacement reaction Methods 0.000 claims abstract description 25
- 238000003384 imaging method Methods 0.000 claims abstract description 22
- 230000005499 meniscus Effects 0.000 claims description 19
- 230000003287 optical effect Effects 0.000 claims description 16
- 238000001514 detection method Methods 0.000 abstract description 11
- 238000003745 diagnosis Methods 0.000 abstract description 5
- 238000012014 optical coherence tomography Methods 0.000 description 24
- 238000000034 method Methods 0.000 description 9
- 239000000463 material Substances 0.000 description 6
- 230000004075 alteration Effects 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- 239000000835 fiber Substances 0.000 description 3
- 230000035515 penetration Effects 0.000 description 3
- 238000001228 spectrum Methods 0.000 description 3
- 206010028980 Neoplasm Diseases 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 230000010363 phase shift Effects 0.000 description 2
- 230000010287 polarization Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 238000004624 confocal microscopy Methods 0.000 description 1
- 238000000799 fluorescence microscopy Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 230000003211 malignant effect Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000009022 nonlinear effect Effects 0.000 description 1
- 238000012634 optical imaging Methods 0.000 description 1
- 239000004038 photonic crystal Substances 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
- 229910052724 xenon Inorganic materials 0.000 description 1
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 description 1
Images
Landscapes
- Microscoopes, Condenser (AREA)
Abstract
Description
技术领域technical field
本申请涉及医疗诊断技术领域,具体而言,涉及一种线场共焦OCT装置。The present application relates to the technical field of medical diagnosis, and in particular, to a line-field confocal OCT device.
背景技术Background technique
对于癌症早期诊断,已经开发了使用无创成像技术的诊断方式,以提供更早、更准确的恶性病变检测。For early cancer diagnosis, diagnostic modalities using non-invasive imaging techniques have been developed to provide earlier and more accurate detection of malignant lesions.
例如能够以最高空间分辨率进行体内皮肤成像的临床可用技术是反射共聚焦显微镜 (RCM)、光学相干断层扫描 (OCT) 和荧光显微镜。RCM是一种光学技术,可提供组织的正面截面图,其空间分辨率与组织学相当,约为1μm。RCM已被证明可以提高诊断的准确性。然而,RCM 的主要限制是在组织中的穿透力相对较弱,只有约200微米,无法对位于组织中的结构进行成像。另一个主要问题是 RCM 切片的解释,因为它们是正面方向,即垂直于传统的垂直方向的组织切片。OCT是一种干涉光学成像模式。OCT产生分辨率为几微米的皮肤横截面图像,明显低于 RCM 。然而,OCT 的皮肤穿透深度比RCM高,约为1毫米。在垂直方向的视图中评估OCT图像的可能性使它们更容易与传统的组织切片进行比较。OCT已应用于各种癌证的诊断。然而,使用OCT诊断不如使用RCM准确,主要是因为OCT的成像分辨率不足。Examples of clinically available techniques capable of imaging in vivo skin with the highest spatial resolution are reflection confocal microscopy (RCM), optical coherence tomography (OCT), and fluorescence microscopy. RCM is an optical technique that provides a frontal cross-sectional view of tissue with a spatial resolution comparable to histology, approximately 1 μm. RCM has been shown to improve diagnostic accuracy. However, the main limitation of RCM is its relatively weak penetration in tissue, only about 200 microns, and cannot image structures located in tissue. Another major issue is the interpretation of RCM slices, as they are in frontal orientation, i.e., tissue slices perpendicular to the traditional vertical orientation. OCT is an interferometric optical imaging modality. OCT produces skin cross-sectional images with a resolution of a few microns, significantly lower than RCM. However, the skin penetration depth of OCT is higher than that of RCM, about 1 mm. The possibility to evaluate OCT images in a vertically oriented view makes them easier to compare with traditional tissue sections. OCT has been applied to the diagnosis of various cancer syndromes. However, diagnosis using OCT is not as accurate as using RCM, mainly because of the insufficient imaging resolution of OCT.
针对上述问题,发明人结合RCM和OCT在空间分辨率、穿透和图像方向方面的优势,提出了一种线场共焦OCT方案。In view of the above problems, the inventors propose a line-field confocal OCT scheme by combining the advantages of RCM and OCT in terms of spatial resolution, penetration and image orientation.
实用新型内容Utility model content
本申请的目的在于提供一种线场共焦OCT装置,具有分辨率高,探测深度大的优点。The purpose of this application is to provide a line-field confocal OCT device, which has the advantages of high resolution and large detection depth.
第一方面,本申请提供了一种线场共焦OCT装置,技术方案如下:In the first aspect, the present application provides a line-field confocal OCT device, and the technical solution is as follows:
包括用于提供线光束的光源模块,还包括:Includes light source module for supplying line beams, also includes:
分束模块,用于接收所述线光束,并将所述线光束分成参考光以及样品光;a beam splitting module for receiving the line beam and splitting the line beam into reference light and sample light;
参考模块,用于接收所述参考光并将所述参考光反射至所述分束模块,所述参考模块连接有用于驱动所述参考模块进行位移的第一位移机构;a reference module, configured to receive the reference light and reflect the reference light to the beam splitting module, the reference module is connected with a first displacement mechanism for driving the reference module to displace;
样品模块,用于接收所述样品光,所述样品光经过所述样品模块进入样品组织内沿光路反射回所述分束模块,所述样品模块连接有用于驱动所述样品模块进行位移的第二位移机构;The sample module is used for receiving the sample light, and the sample light enters the sample tissue through the sample module and is reflected back to the beam splitting module along the optical path, and the sample module is connected with a first module for driving the sample module to displace. Two displacement mechanisms;
成像模块,用于接收被所述参考模块反射回的所述参考光以及被所述样品组织反射回的所述样品光在所述分束模块上形成的干涉光,根据所述干涉光生成图像。an imaging module, configured to receive the reference light reflected back by the reference module and the interference light formed on the beam splitting module by the sample light reflected back by the sample tissue, and generate an image according to the interference light .
利用光源模块来提供线光束,线光束经过分束模块被分为参考光和样品光,参考光经过参考模块后沿着原光路被反射回分束模块,样品光经过样品模块到达样品组织后沿着原光路被反射回分束模块,被反射的参考光和被反射的样品光在分束模块上混合形成干涉光,干涉光照射在成像模块上进而生成图像,在这个过程中,通过第一位移机构移动参考模块,通过第二位移机构移动样品模块进而实现不同深度的探测,并且样品光经过样品模块后,焦点落在样品组织上,经过反射混合形成的干涉光聚焦在成像模块上,结合了RCM和OCT的优势,具有分辨率高,探测深度大的有益效果。The light source module is used to provide a line beam. The line beam is divided into reference light and sample light through the beam splitting module. The reference light passes through the reference module and is reflected back to the beam splitting module along the original optical path. The original optical path is reflected back to the beam splitting module, the reflected reference light and the reflected sample light are mixed on the beam splitting module to form interference light, and the interference light is irradiated on the imaging module to generate an image. The reference module is moved, and the sample module is moved by the second displacement mechanism to realize detection at different depths. After the sample light passes through the sample module, the focus falls on the sample tissue, and the interference light formed by reflection and mixing is focused on the imaging module. Combined with the RCM And the advantages of OCT, it has the beneficial effects of high resolution and large detection depth.
进一步地,在本申请中,所述参考模块包括第一双胶合透镜、第一显微物镜以及反射片,所述第一双胶合透镜用于接收从所述分束模块射出的所述参考光,所述第一显微物镜用于接收经过所述第一双胶合透镜的所述参考光,所述反射片用于接收并反射经过所述第一显微物镜的所述参考光,使所述参考光沿入射光路返回至所述分束模块。Further, in this application, the reference module includes a first doublet lens, a first microscope objective lens and a reflection sheet, and the first doublet lens is used to receive the reference light emitted from the beam splitting module , the first microscope objective lens is used for receiving the reference light passing through the first doublet lens, and the reflection sheet is used for receiving and reflecting the reference light passing through the first microscope objective lens, so that all The reference light is returned to the beam splitting module along the incident light path.
通过第一双胶合透镜来消除色差,进而提高成像质量。The chromatic aberration is eliminated by the first doublet lens, thereby improving the image quality.
进一步地,在本申请中,所述第一双胶合透镜由第一双凸透镜以及第一凹凸透镜胶合而成,所述第一双凸透镜设置在靠近所述分束模块一侧,所述第一双凸透镜靠近所述分束模块一侧的曲率半径为31.69mm,另一侧的曲率半径为-28.45mm,厚度为8mm,所述第一凹凸透镜靠近所述分束模块一侧的曲率半径为-28.45mm,另一侧的曲率半径为-161.05mm,厚度为4mm。Further, in the present application, the first double cemented lens is formed by cementing a first biconvex lens and a first concave-convex lens, the first biconvex lens is arranged on the side close to the beam splitting module, and the first The curvature radius of one side of the biconvex lens close to the beam splitter module is 31.69mm, the curvature radius of the other side is -28.45mm, and the thickness is 8mm. The curvature radius of the first meniscus lens close to the beam splitter module is -28.45mm, the radius of curvature on the other side is -161.05mm, and the thickness is 4mm.
进一步地,在本申请中,所述样品模块包括第二双胶合透镜、第二显微物镜以及窗口片,所述第二双胶合透镜用于接收从所述分束模块射出的所述样品光,所述第二显微物镜用于接收经过所述第二双胶合透镜的所述样品光,所述窗口片用于接收经过所述第二显微物镜的所述样品光,所述样品光穿过所述窗口片进入所述样品组织内。Further, in this application, the sample module includes a second doublet lens, a second microscope objective lens and a window, and the second doublet lens is used to receive the sample light emitted from the beam splitting module , the second microscope objective lens is used for receiving the sample light passing through the second doublet lens, the window is used for receiving the sample light passing through the second microscope objective lens, the sample light into the sample tissue through the window.
通过第二双胶合透镜来消除色差,进而提升成像质量。Chromatic aberration is eliminated by a second doublet lens, thereby improving image quality.
进一步地,在本申请中,所述第二双胶合透镜由第二双凸透镜以及第二凹凸透镜胶合而成,所述第二双凸透镜设置在靠近所述分束模块一侧,所述第二双凸透镜靠近所述分束模块一侧的曲率半径为31.69mm,另一侧的曲率半径为-28.45mm,厚度为8mm,所述第二凹凸透镜靠近所述分束模块一侧的曲率半径为-28.45mm,另一侧的曲率半径为-161.05mm,厚度为4mm。Further, in the present application, the second double cemented lens is formed by cementing a second lenticular lens and a second concave-convex lens, the second lenticular lens is arranged on the side close to the beam splitting module, and the second lenticular lens is The curvature radius of one side of the biconvex lens close to the beam splitting module is 31.69mm, the curvature radius of the other side is -28.45mm, and the thickness is 8mm. The curvature radius of the second meniscus lens close to the beam splitting module is -28.45mm, the radius of curvature on the other side is -161.05mm, and the thickness is 4mm.
进一步地,在本申请中,所述成像模块包括第三双胶合透镜以及线阵相机,所述第三双胶合透镜用于接收所述干涉光并将所述干涉光聚焦在所述线阵相机上,所述线阵相机将所述干涉光转化为电信号进而生成图像。Further, in this application, the imaging module includes a third doublet lens and a line scan camera, the third doublet lens is used for receiving the interference light and focusing the interference light on the line scan camera Above, the line scan camera converts the interference light into an electrical signal to generate an image.
进一步地,在本申请中,所述第三双胶合透镜由第三双凸透镜以及第三凹凸透镜胶合而成,所述第三双凸透镜设置在靠近所述分束模块一侧,所述第三双凸透镜靠近所述分束模块一侧的曲率半径为36.27mm,另一侧的曲率半径为-33.8mm,厚度为8mm,所述第三凹凸透镜靠近所述分束模块一侧的曲率半径为-33.8mm,另一侧的曲率半径为-248.86mm,厚度为4mm。Further, in the present application, the third double-cemented lens is formed by cementing a third double-convex lens and a third concave-convex lens, the third double-convex lens is arranged on a side close to the beam splitting module, and the third double-convex lens is The curvature radius of one side of the lenticular lens close to the beam splitter module is 36.27mm, the curvature radius of the other side is -33.8mm, and the thickness is 8mm. The curvature radius of the third meniscus lens close to the beam splitter module is -33.8mm, the radius of curvature on the other side is -248.86mm, and the thickness is 4mm.
进一步地,在本申请中,所述分束模块为非偏振分束立方体。Further, in this application, the beam splitting module is a non-polarized beam splitting cube.
进一步地,在本申请中,所述光源模块包括激光器、第四双胶合透镜以及第五双胶合透镜,所述激光器输出光线至所述第四双胶合透镜,所述第四双胶合透镜将所述光线转变为高斯光束,所述第五双胶合透镜用于接收所述高斯光束并将其聚焦成所述线光束。Further, in this application, the light source module includes a laser, a fourth doublet lens and a fifth doublet lens, the laser outputs light to the fourth doublet lens, and the fourth doublet lens The light is converted into a Gaussian beam, and the fifth doublet lens is used to receive and focus the Gaussian beam into the line beam.
进一步地,在本申请中,所述第四双胶合透镜由第四凸凹透镜以及第四双凸透镜胶合而成,所述第四凸凹透镜设置在靠近所述激光器一侧,所述第四凸凹透镜靠近所述激光器一侧的曲率半径为57.3mm,另一侧的曲率半径为9.545mm,厚度为2mm,所述第四双凸透镜靠近所述激光器一侧的曲率半径为9.545mm,另一侧的曲率半径为-9.545mm,厚度为6.5mm,所述第五双胶合透镜由第五双凸透镜以及第五凹凸透镜胶合而成,所述第五双凸透镜设置在靠近所述第四双胶合透镜一侧,所述第五双凸透镜靠近所述第四双胶合透镜一侧的曲率半径为30.922mm,另一侧的曲率半径为-40.11mm,厚度为6.12mm,所述第五凹凸透镜靠近所述第四双胶合透镜一侧的曲率半径为-40.11mm,另一侧的曲率半径为-254.462mm,厚度为4.18mm,所述第四双胶合透镜与所述第五双胶合透镜的间距为80mm。Further, in the present application, the fourth double cemented lens is formed by cementing a fourth convex-concave lens and a fourth double-convex lens, the fourth convex-concave lens is arranged on the side close to the laser, and the fourth convex-concave lens is The curvature radius of one side close to the laser is 57.3mm, the curvature radius of the other side is 9.545mm, and the thickness is 2mm. The curvature radius of the fourth lenticular lens close to the laser side is 9.545mm, and the other side The radius of curvature is -9.545mm, the thickness is 6.5mm, the fifth doublet lens is cemented by a fifth doublet lens and a fifth concave-convex lens, and the fifth doublet lens is arranged close to the fourth doublet lens. The curvature radius of one side of the fifth biconvex lens close to the fourth doublet lens is 30.922mm, the curvature radius of the other side is -40.11mm, and the thickness is 6.12mm, and the fifth meniscus lens is close to the The radius of curvature of one side of the fourth doublet lens is -40.11mm, the radius of curvature of the other side is -254.462mm, the thickness is 4.18mm, and the distance between the fourth doublet lens and the fifth doublet lens is 80mm .
由上可知,本申请提供的一种线场共焦OCT装置,利用光源模块来提供线光束,线光束经过分束模块被分为参考光和样品光,参考光经过参考模块后沿着原光路被反射回分束模块,样品光经过样品模块到达样品组织后沿着原光路被反射回分束模块,被反射的参考光和被反射的样品光在分束模块上混合形成干涉光,干涉光照射在成像模块上进而生成图像,在这个过程中,通过第一位移机构移动参考模块,通过第二位移机构移动样品模块进而实现不同深度的探测,并且样品光经过样品模块后,焦点落在样品组织上,经过反射混合形成的干涉光聚焦在成像模块上,结合了RCM和OCT的优势,具有分辨率高,探测深度大的有益效果。It can be seen from the above that a line field confocal OCT device provided by the present application uses a light source module to provide a line beam, the line beam is divided into reference light and sample light through the beam splitting module, and the reference light passes through the reference module and follows the original optical path. After being reflected back to the beam splitter module, the sample light passes through the sample module to the sample tissue and then is reflected back to the beam splitter module along the original optical path. The reflected reference light and the reflected sample light are mixed on the beam splitter module to form interference light, and the interference light is irradiated on the beam splitter module. An image is generated on the imaging module. In this process, the reference module is moved by the first displacement mechanism, and the sample module is moved by the second displacement mechanism to realize detection at different depths. After the sample light passes through the sample module, the focus falls on the sample tissue. , the interference light formed by reflection and mixing is focused on the imaging module, which combines the advantages of RCM and OCT, and has the beneficial effects of high resolution and large detection depth.
本申请的其他特征和优点将在随后的说明书阐述,并且,部分地从说明书中变得显而易见,或者通过实施本申请了解。本申请的目的和其他优点可通过在所写的说明书、权利要求书、以及附图中所特别指出的结构来实现和获得。Other features and advantages of the present application will be set forth in the description that follows, and, in part, will be apparent from the description, or learned by practice of the present application. The objectives and other advantages of the application may be realized and attained by the structure particularly pointed out in the written description, claims, and drawings.
附图说明Description of drawings
图1为本申请提供的一种线场共焦OCT装置结构示意图。FIG. 1 is a schematic structural diagram of a line-field confocal OCT device provided by the present application.
图2为本申请提供的一种线场共焦OCT装置结构示意图。FIG. 2 is a schematic structural diagram of a line-field confocal OCT device provided by the present application.
图3为本申请提供的一种线场共焦OCT装置结构示意图。FIG. 3 is a schematic structural diagram of a line-field confocal OCT device provided by the present application.
图4为使用本申请提供的一种线场共焦OCT装置的分辨率-视场尺寸图。FIG. 4 is a resolution-field size diagram of a line-field confocal OCT device provided by the present application.
图中:100、光源模块;200、分束模块;300、参考模块;400、样品模块;500、成像模块;600、第二位移机构;700、第一位移机构;110、激光器;120、第四双胶合透镜;130、第五双胶合透镜;210、非偏振分束立方体;310、第一双胶合透镜;320、第一显微物镜;330、反射片;410、第二双胶合透镜;420、第二显微物镜;430、窗口片;510、第三双胶合透镜;520、线阵相机。In the figure: 100, light source module; 200, beam splitting module; 300, reference module; 400, sample module; 500, imaging module; 600, second displacement mechanism; 700, first displacement mechanism; 110, laser; 120, No. Four doublet lenses; 130, fifth doublet; 210, non-polarized beam splitter cube; 310, first doublet; 320, first microscope objective; 330, reflective sheet; 410, second doublet; 420, the second microscope objective; 430, the window; 510, the third doublet lens; 520, the line scan camera.
具体实施方式Detailed ways
下面将结合本申请中附图,对本申请中的技术方案进行清楚、完整地描述,显然,所描述的实施例仅仅是本申请一部分实施例,而不是全部的实施例。通常在此处附图中描述和示出的本申请的组件可以以各种不同的配置来布置和设计。因此,以下对在附图中提供的本申请的实施例的详细描述并非旨在限制要求保护的本申请的范围,而是仅仅表示本申请的选定实施例。基于本申请的实施例,本领域技术人员在没有做出创造性劳动的前提下所获得的所有其他实施例,都属于本申请保护的范围。The technical solutions in the present application will be clearly and completely described below with reference to the accompanying drawings in the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. The components of the present application generally described and illustrated in the drawings herein may be arranged and designed in a variety of different configurations. Thus, the following detailed description of the embodiments of the application provided in the accompanying drawings is not intended to limit the scope of the application as claimed, but is merely representative of selected embodiments of the application. Based on the embodiments of the present application, all other embodiments obtained by those skilled in the art without creative work fall within the protection scope of the present application.
应注意到:相似的标号和字母在下面的附图中表示类似项,因此,一旦某一项在一个附图中被定义,则在随后的附图中不需要对其进行进一步定义和解释。同时,在本申请的描述中,术语“第一”、“第二”等仅用于区分描述,而不能理解为指示或暗示相对重要性。It should be noted that like numerals and letters refer to like items in the following figures, so once an item is defined in one figure, it does not require further definition and explanation in subsequent figures. Meanwhile, in the description of the present application, the terms "first", "second", etc. are only used to distinguish the description, and cannot be understood as indicating or implying relative importance.
请参照图1至图4,一种线场共焦OCT装置,其技术方案具体包括:Please refer to FIG. 1 to FIG. 4 , a line-field confocal OCT device, the technical solution of which specifically includes:
光源模块100,用于提供线光束;a
分束模块200,用于接收线光束,并将线光束分成参考光以及样品光;a
参考模块300,用于接收参考光并将参考光反射至分束模块200,参考模块300连接有用于驱动参考模块300进行位移的第一位移机构700;The
样品模块400,用于接收样品光,样品光经过样品模块400进入样品组织内沿光路反射回分束模块200,样品模块400连接有用于驱动样品模块400进行位移的第二位移机构600;The
成像模块500,用于接收被参考模块300反射回的参考光以及被样品组织反射回的样品光在分束模块200上形成的干涉光,根据干涉光生成图像。The
通过上述技术方案,利用光源模块100来提供线光束,线光束经过分束模块200被分为参考光和样品光,参考光经过参考模块300后沿着原光路被反射回分束模块200,样品光经过样品模块400到达样品组织后沿着原光路被反射回分束模块200,被反射的参考光和被反射的样品光在分束模块200上混合形成干涉光,干涉光照射在成像模块500上进而生成图像,在这个过程中,通过第一位移机构700移动参考模块300,通过第二位移机构600移动样品模块400进而实现对样品组织不同深度的探测,并且样品光经过样品模块400后,焦点落在样品组织上,经过反射混合形成的干涉光聚焦在成像模块500上,因此结合了RCM和OCT的优势,具有分辨率高,探测深度大的有益效果。Through the above technical solution, the
进一步地,如图2、图3所示,在其中一些实施例中,参考模块300包括第一双胶合透镜310、第一显微物镜320以及反射片330,第一双胶合透镜310用于接收从分束模块200射出的参考光,第一显微物镜320用于接收经过第一双胶合透镜310的参考光,反射片330用于接收并反射经过第一显微物镜320的参考光,使参考光沿入射光路返回至分束模块200。Further, as shown in FIGS. 2 and 3 , in some embodiments, the
通过上述技术方案,第一双胶合透镜310将参考光聚焦在第一显微物镜320上,参考光经过第一显微物镜320后照射在反射片330上,然后沿光路反射回分束模块200,在这个过程中,第一双胶合透镜310用于消除色差,同时将参考光聚焦在第一显微物镜320上,减小损耗,进而提升成像质量。Through the above technical solution, the
其中,在一些具体实施方式中,第一显微物镜320可以采用市场上常用的显微物镜,作为优先方案,本申请中的第一显微物镜320为尼康Nikon CFI Super Fluor系列的20x0.85na的显微物镜。Wherein, in some specific embodiments, the first
其中,在一些具体实施方式中,第一双胶合透镜310由第一双凸透镜以及第一凹凸透镜胶合而成,第一双凸透镜设置在靠近分束模块200一侧,第一双凸透镜靠近分束模块200一侧的曲率半径为31.69mm,另一侧的曲率半径为-28.45mm,厚度为8mm,第一凹凸透镜靠近分束模块200一侧的曲率半径为-28.45mm,另一侧的曲率半径为-161.05mm,厚度为4mm。其中,第一双凸透镜的材料为n-lak22,第一凹凸透镜的材料为n-sf6。并且,第一凹凸透镜与第一显微物镜320的间距为80mm,第一显微物镜320与反射片330的间距为1mm,第一双凸透镜与分束模块200的间距为42.605mm。Wherein, in some specific implementations, the first double-
其中,第一双胶合透镜310的焦距为50mm。The focal length of the
其中,在一些具体实施方式中,反射片330为中性密度反射片,这是一种镀膜的玻璃片,根据薄膜干涉的原理,将一部分光透过,而将另一部分光反射,可以根据需要来调整参考光反射至分束模块200的光照强度。Among them, in some specific embodiments, the
进一步地,在其中一些实施例中,样品模块400包括第二双胶合透镜410、第二显微物镜420以及窗口片430,第二双胶合透镜410用于接收从分束模块200射出的样品光,第二显微物镜420用于接收经过第二双胶合透镜410的样品光,窗口片430用于接收经过第二显微物镜420的样品光,样品光穿过窗口片430进入样品组织内。Further, in some of the embodiments, the
通过上述技术方案,第二双胶合透镜410将样品光聚焦在第二显微物镜420上,第二显微物镜420将样品光再次聚焦,此时样品光被高度聚焦然后穿过窗口片430照射在样品组织上,然后沿光路反射回分束模块200,在这个过程中,第二双胶合透镜410用于将样品光聚焦在第二显微物镜420上,减少损耗,同时起到减少色差的作用,进而提升成像质量。Through the above technical solution, the
其中,在一些具体实施方式中,第二显微物镜420可以采用市场上常用的显微物镜,作为优先方案,本申请中的第二显微物镜420为尼康Nikon CFI Super Fluor系列的20x0.85na的显微物镜。Wherein, in some specific embodiments, the second
其中,在一些具体实施方式中,第二双胶合透镜410由第二双凸透镜以及第二凹凸透镜胶合而成,第二双凸透镜设置在靠近分束模块200一侧,第二双凸透镜靠近分束模块200一侧的曲率半径为31.69mm,另一侧的曲率半径为-28.45mm,厚度为8mm,第二凹凸透镜靠近分束模块200一侧的曲率半径为-28.45mm,另一侧的曲率半径为-161.05mm,厚度为4mm。其中,第二双凸透镜的材料为n-lak22,第二凹凸透镜的材料为n-sf6。并且,第二凹凸透镜与第二显微物镜420的间距为80mm,第二显微物镜420与窗口片430的间距为1mm,第二双凸透镜与分束模块200的间距为42.605mm。Wherein, in some specific embodiments, the second double-
其中,第二双胶合透镜410的焦距为50mm。The focal length of the
其中,窗口片430用于提供保护作用,避免样品光在样品模块400内传输时受到外界环境的干扰。在另一些实施例中也可以去除窗口片430。Among them, the
进一步地,在其中一些实施例中,成像模块500包括第三双胶合透镜510以及线阵相机520,第三双胶合透镜510用于接收干涉光并将干涉光聚焦在线阵相机520上,线阵相机520将干涉光转化为电信号进而生成图像。Further, in some of the embodiments, the
通过上述技术方案,第三双胶合透镜510将干涉光聚焦在线阵相机520上,通过第三双胶合透镜510来进行聚焦以及消除色差,在线场共焦光路中,只有焦平面上的线所发出的光才能到达线阵相机520成像;焦平面以外的线所发出的光线在像面是离焦的,绝大部分无法到达线阵相机520感光元件。因此,焦平面上的观察目标呈现亮色,而非观察点则作为背景呈现黑色,使反差增加,进而让图像更加清晰,第三双胶合透镜510让焦点落在线阵相机520的感光元件上,进而使线阵相机520形成高质量的图像。Through the above technical solution, the
进一步地,在其中一些实施例中,第三双胶合透镜510由第三双凸透镜以及第三凹凸透镜胶合而成,第三双凸透镜设置在靠近分束模块200一侧,第三双凸透镜靠近分束模块200一侧的曲率半径为36.27mm,另一侧的曲率半径为-33.8mm,厚度为8mm,第三凹凸透镜靠近分束模块200一侧的曲率半径为-33.8mm,另一侧的曲率半径为-248.86mm,厚度为4mm。其中,第三双凸透镜的材料为n-lak22,第三凹凸透镜的材料为n-sf6。并且,第三凹凸透镜与线阵相机520的间距为52.955mm,第三双凸透镜与分束模块200的间距为52.955mm。Further, in some of the embodiments, the third double-
其中,在一些具体实施方式中,第三双胶合透镜510的焦距为60mm。Wherein, in some specific embodiments, the focal length of the
其中,作为优先实施方式,线阵相机520可以采用e2v公司的2048像素cmos相机EV71YO1CUB2210-BB1,此外,也可以用面阵相机来代替线阵相机520。Among them, as a preferred embodiment, the
进一步地,在其中一些实施例中,分束模块200为非偏振分束立方体210。Further, in some of the embodiments, the
通过上述技术方案,采用非偏振分束立方体210可以不管光的波长和偏振态,都会以相同的比率将光线分成两个方向,进而形成参照光和样品光,用于后续形成干涉光,并通过干涉光成像。Through the above technical solution, the non-polarization
进一步地,在其中一些实施例中,光源模块100包括激光器110、第四双胶合透镜120以及第五双胶合透镜130,激光器110用于输出光线至第四双胶合透镜120,第四双胶合透镜120用于将光线转变为高斯光束,第五双胶合透镜130用于接收高斯光束并将其聚焦成线光束。其中,在一些具体实施方式中,可以实用氙气灯代替激光器110作为光源。Further, in some of the embodiments, the
通过上述技术方案,由激光器110产生光线,其中,激光器110可以是超连续谱激光器,使用超短脉冲激光耦合进高非线性光纤,通常是光子晶体光纤PCF,因为光纤的非线性效应、四波混频及光孤子效应,使得输出光的脉冲光谱展宽,谱宽从0.4um~2.4um,从而实现超宽的光谱输出,将光线发射至第四双胶合透镜120上,第四双胶合透镜120将光线转变成近似平行光的高斯光束,高斯光束照射在第五双胶合透镜130上,第五双胶合透镜130将高斯光束聚焦成线光束并输出。Through the above technical solution, light is generated by the
其中,在一些具体实施方式中,第四双胶合透镜120由第四凸凹透镜以及第四双凸透镜胶合而成,第四凸凹透镜设置在靠近激光器110一侧,第四凸凹透镜靠近激光器110一侧的曲率半径为57.3mm,另一侧的曲率半径为9.545mm,厚度为2mm,第四双凸透镜靠近激光器110一侧的曲率半径为9.545mm,另一侧的曲率半径为-9.545mm,厚度为6.5mm,第五双胶合透镜130由第五双凸透镜以及第五凹凸透镜胶合而成,第五双凸透镜设置在靠近第四双胶合透镜120一侧,第五双凸透镜靠近第四双胶合透镜120一侧的曲率半径为30.922mm,另一侧的曲率半径为-40.11mm,厚度为6.12mm,第五凹凸透镜靠近第四双胶合透镜120一侧的曲率半径为-40.11mm,另一侧的曲率半径为-254.462mm,厚度为4.18mm,第四双胶合透镜120与所述第五双胶合透镜130的间距为80mm,第五双胶合透镜130与分束模块200的间距为62.605mm。Wherein, in some specific implementations, the fourth double cemented
其中,在一些具体实施方式中,第四双胶合透镜120的焦距为15mm,第五双胶合透镜130的焦距为75mm。Wherein, in some specific embodiments, the focal length of the
具体的,作为优先方案,在本申请中,光线从激光器110发出后到窗口片430的光学系统参数如下表所示:Specifically, as a preferred solution, in this application, the optical system parameters of the light emitted from the
光线从激光器110发出后到反射片330的光线系统参数如下表所示:The light system parameters of the light emitted from the
干涉光从分束模块220到线阵相机520的光学系统参数如下表所示:The optical system parameters of the interference light from the beam splitting module 220 to the
此外,在一些具体实施方式中,第一位移机构700以及第二位移机构600为PZT移动台,为了保证成像清晰度,第二位移机构600带动第二显微物镜420以及窗口片430进行移动,进而对样品组织进行全深度扫描。其中,PZT平移台是以压电陶瓷作为基础元件驱动的压电平移台,驱动形式为机构放大式。相同驱动电压下,机构放大平台的位移是直驱平台位移的几倍至几十倍,具有纳米级分辨率以及毫秒级的响应时间。In addition, in some specific embodiments, the
第一位移机构700带动第一显微物镜320以及反射片330进行移动,其移动位置根据第二位移机构600带动第二显微物镜420以及窗口片430的移动位置来决定。The
其中,由于第一位移机构700带动第一显微物镜320进行移动,因此,在一些实施例中,第一显微物镜320与第一双胶合透镜310的间距为80mm-80.7mm。Wherein, since the
同理,第二显微物镜420与第二双胶合透镜410的间距为80mm-80.7mm。Similarly, the distance between the second
并且,在本申请的方案中,采用了5帧相移法提高图像的信噪比。该算法需要由5个相关的帧E1、E2、E3、E4、E5计算而来,根据(E4−E2)^2−(E1− E3)(E3− E5),每两个相邻帧之间的相位差为π∕2。在一些实施例中,光源的中心波长是800nm,样品组织的折射率设为1.5,那么每帧需要第一位移机构700移动的距离则是800/(4*1.5)=133.3nm。这种位移产生了π∕2的光学相移。在第二位移机构600执行深度扫描的全过程中,第一位移机构700都要如此震荡运行。其中,在上述公式中,一个波长是2Pi,因此pi/2对应的距离需要除以4。Moreover, in the solution of the present application, a 5-frame phase shift method is adopted to improve the signal-to-noise ratio of the image. The algorithm needs to be calculated from five related frames E1, E2, E3, E4, and E5. According to (E4−E2)^2−(E1− E3)(E3− E5), between every two adjacent frames The phase difference is π∕2. In some embodiments, the central wavelength of the light source is 800 nm, and the refractive index of the sample tissue is set to 1.5, then the distance that the
本申请的技术方案与传统的OCT相比,使用超宽带的光源来提供探测用的光,通过第五双胶合透镜130来聚焦光线,只进行一维成像,从而提高了信噪比,并且不需要实用光栅、光谱仪、扫频激光器等昂贵的光谱器件,在保证探测质量的同时有效降低了成本。Compared with the traditional OCT, the technical solution of the present application uses an ultra-broadband light source to provide light for detection, focuses the light through the
具体成像效果如图4所示,通过本申请的方案,分辨率可以达到1.4um,视场为0.98mm。The specific imaging effect is shown in Fig. 4. With the solution of the present application, the resolution can reach 1.4um, and the field of view is 0.98mm.
以上所述仅为本申请的实施例而已,并不用于限制本申请的保护范围,对于本领域的技术人员来说,本申请可以有各种更改和变化。凡在本申请的精神和原则之内,所作的任何修改、等同替换、改进等,均应包含在本申请的保护范围之内。The above descriptions are merely examples of the present application, and are not intended to limit the protection scope of the present application. For those skilled in the art, various modifications and changes may be made to the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of this application shall be included within the protection scope of this application.
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202122247018.XU CN216365007U (en) | 2021-09-16 | 2021-09-16 | Line field confocal OCT device |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202122247018.XU CN216365007U (en) | 2021-09-16 | 2021-09-16 | Line field confocal OCT device |
Publications (1)
Publication Number | Publication Date |
---|---|
CN216365007U true CN216365007U (en) | 2022-04-26 |
Family
ID=81241120
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202122247018.XU Withdrawn - After Issue CN216365007U (en) | 2021-09-16 | 2021-09-16 | Line field confocal OCT device |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN216365007U (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113712514A (en) * | 2021-09-16 | 2021-11-30 | 佛山光微科技有限公司 | Line field confocal OCT device |
-
2021
- 2021-09-16 CN CN202122247018.XU patent/CN216365007U/en not_active Withdrawn - After Issue
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113712514A (en) * | 2021-09-16 | 2021-11-30 | 佛山光微科技有限公司 | Line field confocal OCT device |
CN113712514B (en) * | 2021-09-16 | 2025-01-07 | 佛山光微科技有限公司 | A Line-field Confocal OCT Device |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN106547079B (en) | Real-time three-dimensional laser fluorescence microscopic imaging device | |
CN102841083B (en) | Method and system of laser scanning phase-microscope imaging | |
US6088097A (en) | Point-scanning luminescent microscope | |
CN108303806B (en) | Depth imaging super-resolution microscopic imaging system | |
CN112666698B (en) | Dispersive super-surface-based fiber bundle multi-azimuth three-dimensional confocal imaging device and method | |
KR101089292B1 (en) | Biomedical Reflex / Fluorescent Compounds Conformal Laser Scanning Microscope | |
US20090021746A1 (en) | Tomography apparatus | |
CN111474694B (en) | A micro endoscope with a large field of view | |
CN101587238B (en) | Two-color two-photon fluorescence imaging method and device | |
JP5547868B2 (en) | Microscope system and method using the same | |
JP6491819B2 (en) | Use of microscope oil immersion objectives and microscope oil immersion objectives | |
CN101485558A (en) | Single-optical fiber multiphoton fluorescence scanning endoscope | |
CN114527102B (en) | A near-infrared two-zone microscopic imaging system and method based on laser scanning | |
CN113835208B (en) | Large-view-field two-photon scanning and imaging device | |
CN105954862A (en) | Microscopic lens and sample locking system based on 4Pi microscope framework | |
CN101819319B (en) | Fluorescence microscopy method and device for generating multilayer polished section by using Fresnel biprism | |
CN210572988U (en) | Head-mounted microscope with high fluorescence collection rate | |
CN211014821U (en) | Microscope | |
CN216365007U (en) | Line field confocal OCT device | |
CN110623641A (en) | A self-adaptive second and third harmonic joint detection microscopic imaging method and device | |
CN108362646A (en) | A kind of system of miniature opto-acoustic microscopic imaging head, production method and its composition | |
CN113712514B (en) | A Line-field Confocal OCT Device | |
CN107478628A (en) | A kind of two-photon fluorescence microscopic method and device based on photon restructuring | |
CN110664369B (en) | Self-adaptive confocal line scanning harmonic microscopic imaging method and device | |
CN102692702A (en) | Confocal microscope using laser interference fields |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
GR01 | Patent grant | ||
GR01 | Patent grant | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: A Line Field Confocal OCT Device Effective date of registration: 20231013 Granted publication date: 20220426 Pledgee: Shanghai Pudong Development Bank Co.,Ltd. Foshan Branch Pledgor: Foshan Light Micro Technology Co.,Ltd. Registration number: Y2023980061066 |
|
AV01 | Patent right actively abandoned | ||
AV01 | Patent right actively abandoned | ||
AV01 | Patent right actively abandoned |
Granted publication date: 20220426 Effective date of abandoning: 20250107 |
|
AV01 | Patent right actively abandoned |
Granted publication date: 20220426 Effective date of abandoning: 20250107 |