CN216351514U

CN216351514U - Observation device for cell fusion

Info

Publication number: CN216351514U
Application number: CN202122813945.3U
Authority: CN
Inventors: 张尚权; 刘晓燕; 关战军
Original assignee: Shanghai Ruizhenmai Biomedical Technology Co ltd
Current assignee: Shanghai Ruizhenmai Biomedical Technology Co ltd
Priority date: 2021-11-17
Filing date: 2021-11-17
Publication date: 2022-04-19
Anticipated expiration: 2031-11-17

Abstract

The utility model relates to the technical field of cell observation, and discloses an observation device for cell fusion, which comprises: microscope body, culture dish and place the platform, the spread groove has been seted up at place the platform's top, the inner wall joint of spread groove has the connecting block, the both sides of spread groove inner wall are provided with latch mechanism. This viewing device that cell fuses usefulness, through setting up the spread groove, the connecting block, clamping mechanism, the mounting panel, the standing groove, observation groove and slewing mechanism, place the standing groove inner wall at the mounting panel with the culture dish of experimental group, clamping mechanism fixes the connecting block, and then the position of fixed mounting panel, the connecting block passes through coupling mechanism and is connected with the mounting panel rotation, it can drive the culture dish motion to rotate the mounting panel, can observe the culture dish and the record data of experimental group in order, change culture dish that need not stop, and convenient for operation has reached the state that cell fuses in the quick observation culture dish, and the record data.

Description

Observation device for cell fusion

Technical Field

The utility model relates to the technical field of cell observation, in particular to an observation device for cell fusion.

Background

Cell fusion is a cytogenetic term, two cells or protoplasts are fused to form a hybrid cell under spontaneous or artificial induction, the basic process comprises the steps of cell fusion to form a heterokaryon, the heterokaryon is subjected to nuclear fusion through mitosis of cells, and finally a mononuclear hybrid cell is formed.

The artificial cell fusion generally adopts the induction technology of chemical and physical factors, the cell wall of the fusion of plant and microbial cells must be removed firstly to enable the cell wall to become a protoplast, the cell wall can be spontaneously fused to form a fusion cell in the mixed culture process, an experiment contrast group with different variables needs to be set in the cell fusion culture process, when a microscope is used for observation, culture dishes need to be placed on the microscope one by one for observation, the observation process is complex in operation, and the observation cannot be carried out quickly and the experiment result can not be recorded.

SUMMERY OF THE UTILITY MODEL

In view of the deficiencies of the prior art, the present invention provides an observation device for cell fusion that solves the problems mentioned in the background above.

The utility model provides the following technical scheme: an observation device for cell fusion, comprising: microscope body, culture dish and place the platform, the spread groove has been seted up at place the platform's top, the inner wall joint of spread groove has the connecting block, the both sides of spread groove inner wall are provided with latch mechanism, the top of connecting block is provided with slewing mechanism, slewing mechanism's surface mounting has the mounting panel, the standing groove has been seted up at the top of mounting panel, culture dish swing joint is at the inner wall of standing groove, the observation groove has been seted up to the bottom of standing groove inner wall, the top of mounting panel is provided with fixed establishment, fixed establishment swing joint is at the top of culture dish, place the platform's top fixedly connected with positioning mechanism, the constant head tank has been seted up on the surface of mounting panel, the inner wall of positioning mechanism joint at the constant head tank.

Preferably, the number of the placing grooves is five, and the five placing grooves are distributed in an annular array by taking the center of the mounting plate as an array center.

Preferably, the clamping mechanism includes spring and fixture block, the circular slot has all been seted up to the both sides of spread groove inner wall, the one end of spring and the inner wall fixed connection of circular slot, the other end of spring and the fixed surface of fixture block are connected, fixture block sliding connection is at the inner wall of circular slot, the draw-in groove has all been seted up to the both sides of connecting block, the fixture block joint is at the inner wall of draw-in groove.

Preferably, slewing mechanism includes connecting axle and bearing, the bottom at the mounting panel is inlayed to the bearing, connecting axle fixed connection is at the inner wall of bearing, connecting axle fixed connection is at the top of connecting block.

Preferably, fixed establishment includes the connecting seat, the inner wall fixedly connected with fixed axle of connecting seat, and the surface activity of fixed axle has cup jointed the torsional spring, the depression bar has been cup jointed in the surface activity of torsional spring, the one end of torsional spring and the inner wall fixed connection of connecting seat, the other end of torsional spring and the inner wall fixed connection of depression bar, the fixed surface of depression bar is connected with the spliced pole, the surface rotation of spliced pole is connected with the roll collar, the both sides of roll collar all are provided with the spacing ring, spacing ring fixed connection is on the surface of spliced pole, roll collar swing joint is at the top of culture dish.

Preferably, the positioning mechanism comprises a connecting rod, an elastic sheet and a positioning block, the connecting rod is fixedly connected to the top of the placing platform, the elastic sheet is fixedly connected to the surface of the connecting rod, the positioning block is fixedly connected to one end of the elastic sheet, and the positioning block is clamped to the inner wall of the positioning groove.

Compared with the prior art, the utility model has the following beneficial effects:

1. this viewing device that cell fuses usefulness, through setting up the spread groove, the connecting block, clamping mechanism, the mounting panel, the standing groove, observation groove and slewing mechanism, place the standing groove inner wall at the mounting panel with the culture dish of experimental group, clamping mechanism fixes the connecting block, and then the position of fixed mounting panel, the connecting block passes through coupling mechanism and is connected with the mounting panel rotation, it can drive the culture dish motion to rotate the mounting panel, can observe the culture dish and the record data of experimental group in order, change culture dish that need not stop, and convenient for operation has reached the state that cell fuses in the quick observation culture dish, and the record data.

2. This viewing device that cell fusion used, through setting up the connecting rod, the shell fragment, locating piece and constant head tank, the surface at the mounting panel is seted up to the constant head tank, and the position of constant head tank is corresponding with the position of placing the groove, rotate the mounting panel, when the constant head tank rotates the position department to the locating piece, the shell fragment promotes the inner wall of locating piece to the constant head tank, it is spacing to carry out the mounting panel, and then the position of location culture dish, the center department coincidence of logical unthreaded hole on observation groove on the mounting panel and the place the platform this moment, the position of culture dish can be fixed a position fast, the speed of observing is improved.

Drawings

FIG. 1 is a schematic perspective view of the present invention;

FIG. 2 is an enlarged view of the structure at A in FIG. 1 according to the present invention;

FIG. 3 is a schematic view of an exploded structure of the securing mechanism of the present invention;

FIG. 4 is a schematic side sectional view of the rotating mechanism of the present invention;

FIG. 5 is an enlarged view of B in FIG. 4 according to the present invention.

In the figure: 1. a microscope body; 2. a culture dish; 3. placing a platform; 4. connecting grooves; 5. connecting blocks; 6. a clamping mechanism; 61. a spring; 62. a clamping block; 63. a circular groove; 64. a card slot; 7. a rotating mechanism; 71. a connecting shaft; 72. a bearing; 8. mounting a plate; 9. a placement groove; 10. an observation tank; 11. a fixing mechanism; 111. a connecting seat; 112. a torsion spring; 113. a pressure lever; 114. connecting columns; 115. a rolling ring; 116. a limiting ring; 12. a positioning mechanism; 121. a connecting rod; 122. a spring plate; 123. positioning blocks; 13. and (6) positioning a groove.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-5, an observation device for cell fusion includes: microscope body 1, culture dish 2 and place the platform 3, spread groove 4 has been seted up at place the platform 3's top, spread groove 4's inner wall joint has connecting block 5, the both sides of spread groove 4 inner wall are provided with latch mechanism 6, the top of connecting block 5 is provided with slewing mechanism 7, slewing mechanism 7's surface mounting has mounting panel 8, standing groove 9 has been seted up at mounting panel 8's top, 2 swing joint of culture dish are at the inner wall of standing groove 9, observation groove 10 has been seted up to the bottom of standing groove 9 inner wall, mounting panel 8's top is provided with fixing mechanism 11, fixing mechanism 11 swing joint is at the top of culture dish 2, place the platform 3's top fixedly connected with positioning mechanism 12, constant head tank 13 has been seted up on mounting panel 8's surface, positioning mechanism 12 joint is at the inner wall of constant head tank 13.

Wherein; the number of the placing grooves 9 is five, and the five placing grooves 9 are distributed in an annular array by taking the center of the mounting plate 8 as the center of the array.

Wherein; clamping mechanism 6 includes spring 61 and fixture block 62, and circular slot 63 has all been seted up to the both sides of connecting groove 4 inner wall, and the one end of spring 61 and the inner wall fixed connection of circular slot 63, the other end of spring 61 and the fixed surface of fixture block 62 are connected, and fixture block 62 sliding connection is at the inner wall of circular slot 63, and draw-in groove 64 has all been seted up to the both sides of connecting block 5, and fixture block 62 joint is at the inner wall of draw-in groove 64.

Wherein; the rotating mechanism 7 comprises a connecting shaft 71 and a bearing 72, the bearing 72 is embedded at the bottom of the mounting plate 8, the connecting shaft 71 is fixedly connected to the inner wall of the bearing 72, and the connecting shaft 71 is fixedly connected to the top of the connecting block 5.

Wherein; the fixing mechanism 11 comprises a connecting seat 111, a fixing shaft is fixedly connected with the inner wall of the connecting seat 111, and the surface of the fixed shaft is movably sleeved with a torsion spring 112, the surface of the torsion spring 112 is movably sleeved with a pressure lever 113, one end of the torsion spring 112 is fixedly connected with the inner wall of the connecting seat 111, the other end of the torsion spring 112 is fixedly connected with the inner wall of the pressure lever 113, the surface of the pressure lever 113 is fixedly connected with a connecting column 114, the surface of the connecting column 114 is rotatably connected with a rolling ring 115, two sides of the rolling ring 115 are respectively provided with a limit ring 116, the limit ring 116 is fixedly connected with the surface of the connecting column 114, the rolling ring 115 is movably connected with the top of the culture dish 2, the pressure lever 113 is lifted by hand, the pressure lever 113 lifts the torsion spring 112, the culture dish 2 of the experimental group is placed on the inner wall of the placing groove 9 of the mounting plate 8, the pressure lever 113 is loosened, the torsion spring 112 resets to drive the pressure lever 113 to press the culture dish 2 to drive the connecting column 114 to move, and the connecting column 114 drives the rolling ring 115 to press the culture dish 2.

Wherein; positioning mechanism 12 includes connecting rod 121, shell fragment 122 and locating piece 123, and connecting rod 121 fixed connection connects at the top of place the platform 3, and shell fragment 122 fixed connection is on the surface of connecting rod 121, and locating piece 123 fixed connection is in the one end of shell fragment 122, and locating piece 123 joint is at the inner wall of constant head tank 13.

The working principle is that the press rod 113 is lifted by hand, the press rod 113 lifts the torsion spring 112, the culture dish 2 of the experimental group is placed on the inner wall of the placing groove 9 of the mounting plate 8, the press rod 113 is loosened, the torsion spring 112 resets to drive the press rod 113 to press the culture dish 2 and drive the connecting column 114 to move, the connecting column 114 drives the rolling ring 115 to press the culture dish 2, then the connecting block 5 is clamped into the inner wall of the connecting groove 4, when the connecting block 5 enters the connecting groove 4, the connecting block 5 extrudes the fixture block 62 to extrude the fixture block 62 into the inner wall of the circular groove 63, meanwhile, the fixture block 62 compresses the spring 61, when the connecting block 5 completely enters the connecting groove 4, the position of the clamping groove 64 to the fixture block 62 is aligned, the spring 61 stretches back and pushes the fixture block 62 to be clamped into the inner wall of the clamping groove 64 to fix the connecting block 5, and further fix the position of the mounting plate 8, the connecting block 5 is rotatably connected with the mounting plate 8 through the connecting mechanism, the mounting plate 8 is rotated, and the mounting plate 8 can drive the culture dish 2 to move, can observe culture dish 2 and the record data of experiment group in order, change culture dish 2 that need not stop, high durability and convenient operation, the state of observing the interior cell fusion of culture dish 2 fast has been reached, and the record data, constant head tank 13 is seted up on the surface of mounting panel 8, and the position of constant head tank 13 is corresponding with the position of standing groove 9, rotate mounting panel 8, when constant head tank 13 rotates the position department to locating piece 123, shell fragment 122 promotes locating piece 123 to the inner wall of constant head tank 13, it is spacing to carry out mounting panel 8, and then the position of location culture dish 2, the coincidence of the center department of light passing through hole on observation tank 10 on the mounting panel 8 and the place the platform 3 this moment, can fix a position culture dish 2's position fast, improve the speed of observing.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims

1. An observation device for cell fusion, comprising: the microscope comprises a microscope body (1), a culture dish (2) and a placement platform (3), wherein a connecting groove (4) is formed in the top of the placement platform (3), a connecting block (5) is connected to the inner wall of the connecting groove (4) in a clamping manner, clamping mechanisms (6) are arranged on two sides of the inner wall of the connecting groove (4), a rotating mechanism (7) is arranged on the top of the connecting block (5), a mounting plate (8) is mounted on the surface of the rotating mechanism (7), a placement groove (9) is formed in the top of the mounting plate (8), the culture dish (2) is movably connected to the inner wall of the placement groove (9), an observation groove (10) is formed in the bottom of the inner wall of the placement groove (9), a fixing mechanism (11) is arranged on the top of the mounting plate (8), the fixing mechanism (11) is movably connected to the top of the culture dish (2), and a positioning mechanism (12) is fixedly connected to the top of the placement platform (3), constant head tank (13) have been seted up on the surface of mounting panel (8), positioning mechanism (12) joint is at the inner wall of constant head tank (13).

2. The observation apparatus for cell fusion as defined in claim 1, wherein the number of the placement grooves (9) is five, and the five placement grooves (9) are distributed in a circular array with the center of the mounting plate (8) as the center of the array.

3. The observation device for cell fusion according to claim 1, wherein the clamping mechanism (6) comprises a spring (61) and a clamping block (62), circular grooves (63) are respectively formed on two sides of the inner wall of the connecting groove (4), one end of the spring (61) is fixedly connected with the inner wall of the circular groove (63), the other end of the spring (61) is fixedly connected with the surface of the clamping block (62), the clamping block (62) is slidably connected with the inner wall of the circular groove (63), clamping grooves (64) are respectively formed on two sides of the connecting block (5), and the clamping block (62) is clamped on the inner wall of the clamping grooves (64).

4. The observation device for cell fusion as claimed in claim 1, wherein the rotation mechanism (7) comprises a connecting shaft (71) and a bearing (72), the bearing (72) is embedded at the bottom of the mounting plate (8), the connecting shaft (71) is fixedly connected to the inner wall of the bearing (72), and the connecting shaft (71) is fixedly connected to the top of the connecting block (5).

5. The observation apparatus for cell fusion according to claim 1, the fixing mechanism (11) comprises a connecting seat (111), the inner wall of the connecting seat (111) is fixedly connected with a fixing shaft, and the surface of the fixed shaft is movably sleeved with a torsion spring (112), the surface of the torsion spring (112) is movably sleeved with a pressure lever (113), one end of the torsion spring (112) is fixedly connected with the inner wall of the connecting seat (111), the other end of the torsion spring (112) is fixedly connected with the inner wall of the pressure lever (113), a connecting column (114) is fixedly connected to the surface of the pressure lever (113), a rolling ring (115) is rotatably connected to the surface of the connecting column (114), both sides of the rolling ring (115) are provided with limiting rings (116), the limiting rings (116) are fixedly connected to the surface of the connecting column (114), and the rolling ring (115) is movably connected to the top of the culture dish (2).

6. The observation device for cell fusion according to claim 1, wherein the positioning mechanism (12) comprises a connecting rod (121), a spring plate (122) and a positioning block (123), the connecting rod (121) is fixedly connected to the top of the placement platform (3), the spring plate (122) is fixedly connected to the surface of the connecting rod (121), the positioning block (123) is fixedly connected to one end of the spring plate (122), and the positioning block (123) is clamped to the inner wall of the positioning groove (13).