CN216350544U - Detection equipment for thin-layer chromatography - Google Patents

Abstract

The application relates to the technical field of biological detection, and particularly discloses a detection device for thin-layer chromatography. Including expansion device and chromatogram device, still include washing unit, washing unit includes workstation, supporting seat, the subassembly that slides and is used for drawing the extraction element of spot, the supporting seat sets up on the workstation, the extraction element slides through the subassembly level that slides and connects on the supporting seat. The spot need not the manual work at the in-process that detects to extract by this application, has the effect that improves stationary phase detection efficiency.

Description

Detection equipment for thin-layer chromatography

Technical Field

The application relates to the technical field of biological detection, in particular to a detection device for thin-layer chromatography.

Background

Thin layer chromatography is a chromatographic method in which a suitable stationary phase is coated on a thin layer plate to form a uniform thin layer on the thin layer plate, and then the stationary phase is developed, separated, identified and quantified using a suitable solvent as a mobile phase.

When the developing solution is used for displaying the stationary phase, sometimes spots appear on the thin-layer plate due to the high or low PH of the developing solution, and the spots need to be extracted and analyzed. When the spot is extracted, a tester generally extracts the spot, and the detection efficiency of the stationary phase is influenced by using a manual extraction mode.

SUMMERY OF THE UTILITY MODEL

In order to improve the detection efficiency of the stationary phase, the application provides a detection device for thin layer chromatography.

The application provides a check out test set for thin layer chromatography adopts following technical scheme:

the utility model provides a check out test set for thin-layer chromatography, is including expandeing device and chromatographic device, still includes washing unit, washing unit includes workstation, supporting seat, the subassembly that slides and is used for drawing the extraction element of spot, the supporting seat sets up on the workstation, the extraction element slides through the subassembly level that slides and connects on the supporting seat.

By adopting the technical scheme, the thin-layer plate is unfolded by using the unfolding device, then the unfolded thin-layer plate is placed on the workbench, the sliding component is used for sliding the extraction component, the extraction component is used for extracting spots on the thin-layer plate, and finally the chromatographic device is used for detecting the extracted spot liquid; need not artifical and draw in the testing process, improved the detection efficiency of stationary phase.

Optionally, the extraction assembly comprises an extraction cylinder and an extraction head, the cylinder body of the extraction cylinder is horizontally connected to the supporting seat in a sliding mode through the sliding assembly, and the extraction head is connected to a piston rod of the extraction cylinder.

By adopting the technical scheme, the extraction solvent is firstly added into the extraction head, then the extraction cylinder is started, and the piston rod of the extraction cylinder drives the extraction head to extract the spots on the thin-layer plate.

It is optional, the subassembly that slides includes sliding block, threaded rod, bevel gear group and dwang, be equipped with the groove of sliding that is used for supplying the sliding block to slide on the supporting seat, the threaded rod level is rotated and is connected at the inslot that slides, the dwang rotates to be connected on the supporting seat, and the dwang is connected with the threaded rod through bevel gear group, sliding block threaded connection is on the threaded rod, the vertical connection of cylinder body of drawing the cylinder is on the sliding block.

Through adopting above-mentioned technical scheme, rotate the dwang, the dwang drives bevel gear group, threaded rod in proper order and rotates, and the threaded rod drives the sliding block and moves at the inslot water level and smoothly that slides, and the sliding block drives and draws the cylinder and slide on the supporting seat level.

Optionally, the bevel gear set comprises a first bevel gear and a second bevel gear, the first bevel gear is connected to the rotating rod, the second bevel gear is connected to the threaded rod, and the second bevel gear is meshed with the first bevel gear.

Through adopting above-mentioned technical scheme, rotate the dwang, the dwang drives first bevel gear, second bevel gear, threaded rod in proper order and rotates, has slowed down slew velocity for draw the head and can fully draw the spot on the thin-layer board.

Optionally, be equipped with adjusting part on the workstation, adjusting part includes adjusting screw, accommodate motor, regulating block and is used for placing the board of accepting of thin-layer board, be equipped with the spout that is used for supplying the regulating block horizontal slip on the workstation, the adjusting screw horizontal rotation is connected in the spout, and adjusting screw and accommodate motor's motor shaft coaxial coupling, regulating block threaded connection is on adjusting screw, accept the board and connect on the regulating block.

Through adopting above-mentioned technical scheme, start adjusting motor, adjusting motor's motor shaft drives adjusting screw and rotates, and adjusting screw drives the regulating block and slides in the spout level, and the regulating block drives accepts the board and slides on the workstation level to be convenient for adjust the position of thin-layer board on the workstation, improved the flexibility that washing unit used.

Optionally, the unfolding device comprises an unfolding box, a sealing cover, a mounting piece for mounting the thin-layer plate and a detector for detecting the front edge of the unfolded liquid level, the sealing cover is covered on the unfolding box, the mounting piece is arranged on the sealing cover, and the detector is connected to the side wall of the unfolding box through the vertical sliding of the lifting piece.

By adopting the technical scheme, firstly, the developing solution is added into the developing box, then the thin-layer plate is arranged on the sealing cover by using the mounting piece, then the thin-layer plate is inserted into the developing box, the sealing cover covers the developing box, the thin-layer plate is contacted with the developing solution, and the developing solution is used for developing and separating the stationary phase on the thin-layer plate through the capillary action; the detector is adjusted to the unfolding height by the lifting piece, when the height of the front edge of the unfolding liquid level reaches the unfolding height, the detector gives an alarm, and a tester takes out the thin-layer plate to complete the unfolding of the thin-layer plate.

Optionally, the lifter includes guide bar, elevator, fixed block and bolt, the vertical setting of guide bar is on the lateral wall of exhibition case, be equipped with on the elevator with the unsmooth complex lift groove of guide bar, the vertical sliding connection of elevator is on the guide bar, be equipped with on the fixed block with the unsmooth complex fixed slot of guide bar, the vertical sliding connection of fixed block is on the guide bar, the fixed block sets up with the elevator relatively, the bolt passes fixed block threaded connection on the elevator, the detector is connected on the elevator.

By adopting the technical scheme, the height of the detector can be adjusted by loosening the bolt and sliding the lifting block; the bolts are tightened so that the fixed block and the lifting block clamp the guide bar, thereby fixing the detector on the guide bar.

Optionally, the installed part includes lifting screw, lifter plate and electronic clamping jaw, the vertical threaded connection of lifting screw is on sealed covering, the lifter plate level is rotated and is connected on lifting screw, and the both ends butt of lifter plate is on the relative inside wall of expansion case, electronic clamping jaw is connected on the lifter plate.

Through adopting above-mentioned technical scheme, utilize electronic clamping jaw centre gripping thin-layer board earlier, rotate the lifting screw rod again, the lifter plate drives the thin-layer board and moves downwards in expanding the incasement for thin-layer board and expansion liquid contact.

In summary, the present application includes at least one of the following beneficial technical effects:

1. in the application, spots do not need to be manually extracted during detection, so that the detection efficiency of the stationary phase is improved;

2. the bevel gear set is utilized to reduce the rotating speed, so that the extracting head can fully extract spots on the thin layer plate;

3. the position of the thin-layer plate on the workbench can be adjusted by the adjusting assembly, and the use flexibility of the flushing device is improved. Drawings

Fig. 1 is a schematic structural diagram for embodying the whole of the detection apparatus in this embodiment.

Fig. 2 is an exploded view of the deployment device of the present embodiment.

Fig. 3 is an enlarged view at a in fig. 2.

Fig. 4 is a schematic structural diagram for embodying the flushing device in the present embodiment.

Fig. 5 is an exploded view of the present embodiment for embodying the adjustment assembly.

Fig. 6 is a schematic structural diagram for embodying the glide assembly in the present embodiment.

Description of reference numerals:

1. a deployment device; 11. unfolding the box; 12. a sealing cover; 13. a mounting member; 131. a lifting screw; 132. a lifting plate; 133. an electric jaw; 134. rotating the block; 14. a detector; 15. a lifting member; 151. a guide bar; 152. a lifting block; 1521. a lifting groove; 153. a fixed block; 1531. fixing grooves; 154. a bolt; 155. a bump; 2. a chromatographic device; 3. a flushing device; 31. a work table; 311. a chute; 32. a supporting seat; 321. a sliding groove; 33. a slipping component; 331. a sliding block; 332. a threaded rod; 333. a bevel gear set; 3331. a first bevel gear; 3332. a second bevel gear; 334. rotating the rod; 335. a rod cap; 34. an extraction component; 341. extracting a cylinder; 342. extracting a head; 35. an adjustment assembly; 351. adjusting the screw rod; 352. adjusting the motor; 353. an adjusting block; 354. a bearing plate.

Detailed Description

The present application is described in further detail below with reference to figures 1-6.

The embodiment of the application discloses a detection device for thin layer chromatography.

Referring to fig. 1, a detection device for thin layer chromatography comprises a developing device 1, a chromatography device 2 and a washing device 3, wherein the developing device 1 comprises a developing box 11, a sealing cover 12, a mounting piece 13 and a detector 14, the developing box 11 is made of transparent glass, the sealing cover 12 is covered on the developing box 11, the mounting piece 13 is arranged on the sealing cover 12, and the detector 14 is vertically connected on the outer side wall of the developing box 11 in a sliding manner through a lifting piece 15.

Referring to fig. 2, the mounting member 13 includes a lifting screw 131, a lifting plate 132 and an electric clamping jaw 133, the lifting screw 131 is vertically screwed on the sealing cover 12, a rotating block 134 is fixedly connected to the top end of the lifting screw 131, the lifting plate 132 is horizontally and rotatably connected to the bottom end of the lifting screw 131, the end surface of the lifting plate 132 abuts against the opposite inner side wall of the unfolding box 11, and the electric clamping jaw 133 is vertically and fixedly connected to the bottom surface of the lifting plate 132; the thin-layer plate is clamped by the electric clamping jaw 133, the lifting screw 131 is rotated by the rotating block 134, the lifting screw 131 drives the lifting plate 132 to move downwards in the unfolding box 11, and the lifting plate 132 drives the thin-layer plate to move downwards in the unfolding box 11, so that the thin-layer plate is contacted with the unfolding liquid.

Referring to fig. 2 and 3, the lifting member 15 includes two guide rods 151 and a lifting block 152, the detector comprises a fixed block 153 and two bolts 154, a convex block 155 horizontally extends on the outer side wall of the unfolding box 11, the two guide rods 151 are oppositely arranged, the guide rods 151 are vertically and fixedly connected to the bottom surface of the convex block 155, a gap exists between the guide rods 151 and the unfolding box 11, a lifting groove 1521 is arranged on the lifting block 152, the lifting groove 1521 is in concave-convex fit with the guide rods 151, the lifting block 152 is vertically and slidably connected between the guide rods 151 and the unfolding box 11, a fixing groove 1531 is arranged on the fixed block 153, the fixing groove 1531 is in concave-convex fit with the guide rods 151, the fixed block 153 is vertically and slidably connected to the guide rods 151, the fixed block 153 is oppositely arranged with the lifting block 152, the bolts 154 penetrate through the fixed block 153 and are in threaded connection with the lifting block 152, the detector 14 is an infrared sensor, and the detector 14 is fixedly connected to the bottom surface of the lifting block 152; loosening the bolts 154 and sliding the lifting block 152 to adjust the height of the detector 14; the bolts 154 are tightened so that the fixing block 153 and the elevating block 152 clamp the guide rod 151, thereby fixing the detector 14 to the guide rod 151.

Referring to fig. 4, the washing device 3 includes a workbench 31, a support seat 32, a sliding assembly 33 and an extracting assembly 34, an adjusting assembly 35 is disposed on the workbench 31, the support seat 32 is fixedly connected to the workbench 31, the extracting assembly 34 is horizontally connected to the support seat 32 by the sliding assembly 33 in a sliding manner, and the extracting assembly 34 is disposed above the adjusting assembly 35.

Referring to fig. 5, the adjusting assembly 35 includes an adjusting screw 351, an adjusting motor 352, an adjusting block 353 and a receiving plate 354, the sliding slot 311 is disposed on the worktable 31, the length direction of the sliding slot 311 is consistent with the width direction of the worktable 31, the adjusting screw 351 is horizontally and rotatably connected in the sliding slot 311, the adjusting motor 352 is horizontally and fixedly connected to the side wall of the worktable 31, the motor shaft of the adjusting motor 352 is coaxially and fixedly connected with the adjusting screw 351, the adjusting block 353 is screwed on the adjusting screw 351, the adjusting block 353 is horizontally and slidably connected in the sliding slot 311, the receiving plate 354 is horizontally and fixedly connected to the top surface of the adjusting block 353, and the receiving plate 354 is horizontally and slidably connected to the worktable 31.

Referring to fig. 5, the unfolded thin-layer plate is placed on the receiving plate 354, the adjusting motor 352 is started, a motor shaft of the adjusting motor 352 drives the adjusting screw 351 to rotate, the adjusting screw 351 drives the adjusting block 353 to horizontally move in the sliding groove 311, and the adjusting block 353 drives the receiving plate 354 to horizontally slide on the workbench 31, so that the position of the thin-layer plate on the workbench 31 can be adjusted conveniently.

Referring to fig. 4 and 6, the sliding assembly 33 includes a sliding block 331, a threaded rod 332, a bevel gear set 333 and a rotating rod 334, a sliding groove 321 is formed in the supporting seat 32, a length direction of the sliding groove 321 is consistent with a length direction of the worktable 31, the threaded rod 332 is horizontally and rotatably connected in the sliding groove 321, the rotating rod 334 is horizontally and rotatably connected in the supporting seat 32, one end of the rotating rod 334 extends into the sliding groove 321 and is connected with the threaded rod 332 through the bevel gear set 333, the other end of the rotating rod 334 extends out of the supporting seat 32, and one end of the rotating rod 334 extending out of the supporting seat 32 is fixedly connected with a rod cap 335.

Referring to fig. 4 and 6, the bevel gear set 333 includes a first bevel gear 3331 and a second bevel gear 3332, the first bevel gear 3331 is fixedly coupled to the rotating rod 334, the second bevel gear 3332 is fixedly coupled to the threaded rod 332, and the second bevel gear 3332 is engaged with the first bevel gear 3331; the sliding block 331 is in threaded connection with the threaded rod 332, and the sliding block 331 is horizontally connected in the sliding groove 321 in a sliding manner; the extraction assembly 34 comprises an extraction cylinder 341 and an extraction head 342, wherein the cylinder body of the extraction cylinder 341 is vertically and fixedly connected to the bottom surface of the sliding block 331, and the extraction head 342 is fixedly connected to the piston rod of the extraction cylinder 341.

Referring to fig. 4 and 6, the extraction solvent is added into the extraction head 342, the extraction cylinder 341 is started, and the piston rod of the extraction cylinder 341 drives the extraction head 342 to move downward, so that the extraction head 342 contacts with the thin-layer plate; then, the rotating rod 334 is rotated by the rod cap 335, the rotating rod 334 sequentially drives the first bevel gear 3331, the second bevel gear 3332 and the threaded rod 332 to rotate, the threaded rod 332 drives the sliding block 331 to horizontally move in the sliding groove 321, the sliding block 331 drives the extraction cylinder 341 to horizontally slide on the supporting seat 32, and the extraction solvent in the extraction head 342 automatically extracts spots.

The implementation principle of the detection equipment for the thin-layer chromatography is as follows: firstly, adding unfolding liquid into the unfolding box 11, clamping the thin-layer plate by using an electric clamping jaw 133, then inserting the thin-layer plate into the unfolding box 11, and covering the unfolding box 11 with a sealing cover 12; the rotating block 134 is used for rotating the lifting screw 131, the lifting screw 131 drives the lifting plate 132 to move downwards in the unfolding box 11, the lifting plate 132 drives the thin-layer plate to move downwards in the unfolding box 11, the thin-layer plate is in contact with unfolding liquid, and the unfolding liquid unfolds and separates a stationary phase on the thin-layer plate through capillary action; loosening the bolts 154, sliding the lifting block 152, adjusting the detector 14 to the unfolded height, and then tightening the bolts 154 so that the fixing block 153 and the lifting block 152 clamp the guide rod 151 to fix the detector 14 on the guide rod 151; when the height of the front edge of the developed liquid level reaches the development height, the detector 14 gives an alarm, and a tester takes out the thin-layer plate to complete the development of the thin-layer plate; placing the unfolded thin-layer plate on the bearing plate 354, starting the adjusting motor 352, driving the adjusting screw 351 to rotate by a motor shaft of the adjusting motor 352, driving the adjusting block 353 to horizontally move in the sliding groove 311 by the adjusting screw 351, and driving the bearing plate 354 to horizontally slide on the workbench 31 by the adjusting block 353, so that spots on the thin-layer plate are positioned below the extracting head 342; adding an extraction solvent into the extraction head 342, starting the extraction cylinder 341, and driving the extraction head 342 to move downwards by a piston rod of the extraction cylinder 341 so that the extraction head 342 is in contact with the thin-layer plate; then, a rotating rod 334 is rotated by a rod cap 335, the rotating rod 334 sequentially drives a first bevel gear 3331, a second bevel gear 3332 and a threaded rod 332 to rotate, the threaded rod 332 drives a sliding block 331 to horizontally move in a sliding groove 321, the sliding block 331 drives an extraction cylinder 341 to horizontally slide on a supporting seat 32, and an extraction solvent in an extraction head 342 automatically extracts spots; finally, the extracted spot liquid is detected by the chromatographic device 2.

The above embodiments are preferred embodiments of the present application, and the protection scope of the present application is not limited by the above embodiments, so: all equivalent changes made according to the structure, shape and principle of the present application shall be covered by the protection scope of the present application.

Claims (8)

1. A detection apparatus for thin layer chromatography comprising a developing device (1) and a chromatography device (2), characterized in that: still include washing unit (3), washing unit (3) include workstation (31), supporting seat (32), slide subassembly (33) and be used for extracting the extraction subassembly (34) of spot, supporting seat (32) set up on workstation (31), it slides through subassembly (33) level and connects on supporting seat (32) to extract subassembly (34).

2. The detection apparatus for thin layer chromatography according to claim 1, characterized in that: the extraction assembly (34) comprises an extraction cylinder (341) and an extraction head (342), the cylinder body of the extraction cylinder (341) is horizontally connected to the supporting seat (32) in a sliding mode through the sliding assembly (33), and the extraction head (342) is connected to the piston rod of the extraction cylinder (341).

3. The detection apparatus for thin layer chromatography according to claim 2, characterized in that: slip subassembly (33) including sliding block (331), threaded rod (332), bevel gear group (333) and dwang (334), be equipped with on supporting seat (32) and be used for supplying sliding block (331) slip groove (321) that slide, threaded rod (332) horizontal rotation is connected in sliding groove (321), dwang (334) are rotated and are connected on supporting seat (32), and dwang (334) are connected with threaded rod (332) through bevel gear group (333), sliding block (331) threaded connection is on threaded rod (332), the vertical connection of cylinder body that draws cylinder (341) is on sliding block (331).

4. A detecting apparatus for thin layer chromatography as set forth in claim 3, wherein: the bevel gear set (333) includes a first bevel gear (3331) and a second bevel gear (3332), the first bevel gear (3331) is connected to the rotating rod (334), the second bevel gear (3332) is connected to the threaded rod (332), and the second bevel gear (3332) is engaged with the first bevel gear (3331).

5. The detection apparatus for thin layer chromatography according to claim 1, characterized in that: be equipped with adjusting part (35) on workstation (31), adjusting part (35) are including adjusting screw (351), accommodate motor (352), regulating block (353) and be used for placing thin-layer board accept board (354), be equipped with on workstation (31) and be used for supplying spout (311) that regulating block (353) level slided, adjusting screw (351) horizontal rotation is connected in spout (311), and adjusting screw (351) and the motor shaft coaxial coupling of adjusting motor (352), regulating block (353) threaded connection is on adjusting screw (351), accept board (354) and connect on regulating block (353).

6. The detection apparatus for thin layer chromatography according to claim 1, characterized in that: unfolding device (1) including unfolding case (11), sealed lid (12), be used for installing installed part (13) of thin-layer board and be used for detecting detector (14) that expand the liquid level forward margin, sealed lid (12) lid is established on unfolding case (11), installed part (13) set up on sealed lid (12), detector (14) are connected on the lateral wall of unfolding case (11) through the vertical sliding of lifter (15).

7. The detection apparatus for thin layer chromatography according to claim 6, characterized in that: lifting member (15) include guide bar (151), elevator (152), fixed block (153) and bolt (154), the vertical setting of guide bar (151) is on the lateral wall of expansion case (11), be equipped with on elevator (152) with guide bar (151) unsmooth complex lift groove (1521), elevator (152) vertical sliding connection is on guide bar (151), be equipped with on fixed block (153) with guide bar (151) unsmooth complex fixed slot (1531), fixed block (153) vertical sliding connection is on guide bar (151), fixed block (153) and elevator (152) set up relatively, bolt (154) pass fixed block (153) threaded connection on elevator (152), detector (14) are connected on elevator (152).

8. The detection apparatus for thin layer chromatography according to claim 6, characterized in that: the installed part (13) includes lifting screw (131), lifter plate (132) and electronic clamping jaw (133), the vertical threaded connection of lifting screw (131) is on sealed lid (12), lifter plate (132) level is rotated and is connected on lifting screw (131), and the both ends butt of lifter plate (132) is on the relative inside wall of expansion case (11), electronic clamping jaw (133) are connected on lifter plate (132).

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