CN216349901U - Gel dyeing and decoloring device - Google Patents

Abstract

The utility model relates to a gel dyeing and decoloring device which comprises a container and a fixing component. The fixed subassembly includes first fixed plate, second fixed plate and a plurality of connecting block, and first fixed plate and second fixed plate are connected in the connecting block, first fixed plate and second fixed plate be used for with the relative both sides butt of gel to fix the gel. When placing fixed subassembly and gel in holding the intracavity and carrying out decoloration, dyeing operation, because the gel is fixed in the centre gripping space that first fixed plate and second fixed plate formed, consequently can reduce to rock and take off the risk that dyeing in-process gel turned over from top to bottom and container wall contact, reduce the cracked possibility of gel, guarantee subsequent experimental analysis effect. After dyeing or decoloration operation are accomplished, take out fixed subassembly from holding the intracavity, then take out first fixed plate from the connecting block, with the slope of second fixed plate again, can realize getting glue, need not artifical manual glue of dragging for, reduce the injury of decoloration liquid and dyeing liquid to the human body.

Description

Gel dyeing and decoloring device

Technical Field

The utility model relates to the technical field of biological medicines, in particular to a gel dyeing and decoloring device.

Background

Polyacrylamide gel electrophoresis (PAGE) is a commonly used electrophoresis technique, is mainly used for separating proteins and oligonucleotides, can further analyze characteristics such as molecular weight, isoelectric point and the like, and is widely applied to the technical field of biological medicines. PAGE takes polyacrylamide gel with molecular sieve function as a medium, and different proteins or oligonucleotides are gradually separated due to the difference of molecular weight, shape and attached charge amount under the action of an electric field. The separated proteins or oligonucleotides are stained and background-removed for better detection and analysis, and therefore, the staining and destaining processes are important components of the PAGE technology.

In the dyeing and decoloring processes, the dyeing and decoloring container with the gel is generally required to be placed on a shaking table for shaking, however, in the shaking process, the gel is easy to turn over up and down in the dyeing and decoloring container and is in contact with the container wall, so that the gel is broken, and subsequent experimental analysis is influenced.

SUMMERY OF THE UTILITY MODEL

In view of the above, it is necessary to provide a gel dyeing and decoloring apparatus which solves the above-mentioned problems, in view of the problem that the conventional gel dyeing and decoloring apparatus is liable to be broken by the turnover of the gel.

A gel dyeing and decoloring device comprises a container and a fixing component; the container is provided with a containing cavity, and the containing cavity is used for containing dyeing liquid or decoloring liquid; the fixing assembly can be placed in the accommodating cavity and comprises a first fixing plate, a second fixing plate and a plurality of connecting blocks, and at least two connecting blocks are respectively connected to two opposite sides of the first fixing plate in the horizontal direction; the one end of connecting block is constructed two joint portions along vertical direction interval, first fixed plate with the second fixed plate corresponds the joint respectively in one joint portion, first fixed plate with the second fixed plate is used for following with the gel the relative both sides butt of vertical direction.

In one embodiment, the clamping portion includes a clamping groove recessed along a horizontal direction, and the first fixing plate and the second fixing plate are respectively clamped with the corresponding clamping groove.

In one embodiment, at least three clamping grooves are arranged at intervals along the vertical direction of the connecting block, and the first fixing plate and/or the second fixing plate can be connected to the clamping grooves at different positions.

In one embodiment, the fixing component is in clearance fit with the cavity wall of the accommodating cavity, and the connecting block can slide along the cavity wall of the accommodating cavity.

In one embodiment, a plurality of positioning columns extend upwards from the bottom wall of the accommodating cavity, and the positioning columns are used for supporting the second fixing plate.

In one embodiment, a plurality of through holes are configured on each of the first fixing plate and the second fixing plate.

In one embodiment, the gel staining and decoloring device further comprises a cover body detachably connected to the top of the container, and the cover body is used for closing the accommodating cavity.

In one embodiment, the two ends of the first fixing plate in the horizontal direction are provided with taking and placing grooves penetrating in the vertical direction.

In one embodiment, the gel dyeing and decoloring device further comprises a clamping piece, wherein the clamping piece is provided with an extending part, and the extending part can penetrate through the access slot and abut against the bottom wall of the first fixing plate.

In one embodiment, the first fixing plate, the second fixing plate and the container are made of transparent materials.

The technical scheme has the following beneficial effects: the gel dyeing and decoloring device comprises a container and a fixing component. Wherein, the container is used for holding dyeing liquid or decoloration liquid, and fixed subassembly includes first fixed plate, second fixed plate and connecting block, and the connecting block structure has joint portion, through first fixed plate and second fixed plate joint in different joint portion respectively for form the centre gripping space between first fixed plate and the second fixed plate, the relative both sides of gel butt respectively in first fixed plate and second fixed plate, thereby fix the gel. After the gel is fixed between the first fixing plate and the second fixing plate, the fixing assembly is placed in the accommodating cavity for decoloring and dyeing. When placing the device and rocking on the shaking table, because the gel is fixed in the centre gripping space that first fixed plate and second fixed plate formed, consequently can reduce the risk that the gel overturns from top to bottom and contacts with the container wall to reduce the cracked possibility of gel, guarantee subsequent experimental analysis effect. After dyeing or decoloration operation are accomplished, take out fixed subassembly from holding the intracavity, then take out first fixed plate from the connecting block for the restriction of gel breaking away from first fixed plate, with the slope of second fixed plate again, the side slope of second fixed plate can be followed to the gel and go out, need not artifical manual glue of dragging for, reduce decoloration liquid and dyeing liquid to the injury of human body, and can reduce the manual risk that drags for gluing and lead to the colloid to break.

Drawings

FIG. 1 is a schematic structural diagram of a gel dyeing and decoloring apparatus according to an embodiment of the present invention;

FIG. 2 is a schematic view of the first retaining plate shown in FIG. 1;

FIG. 3 is a partial schematic view of the gel staining and destaining apparatus shown in FIG. 1;

FIG. 4 is a schematic view of the connection block shown in FIG. 3;

FIG. 5 is a schematic view of a clamping member for clamping the first fixing plate;

fig. 6 is a schematic view of the container and cap shown in fig. 1.

Reference numerals: 100-gel dyeing and decoloring device; 110-a container; 111-a containment chamber; 112-a handle; 113-a positioning column; 120-a first fixation plate; 121-a through hole; 122-pick-and-place slot; 130-a second fixation plate; 140-connecting block; 141-a snap-in part; 150-a cover; 151-a handle; 160-a clamp; 161-an intrusion.

Detailed Description

In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.

In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the utility model and to simplify the description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the utility model.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.

In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

In the present invention, unless otherwise expressly stated or limited, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through an intermediate. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like as used herein are for illustrative purposes only and do not denote a unique embodiment.

Fig. 1 is a schematic structural diagram of a gel dyeing and decoloring apparatus 100 according to an embodiment of the present invention; fig. 3 is a partial schematic view of the gel staining and decoloring apparatus 100 shown in fig. 1. As shown in fig. 1 and 3, an embodiment of the present invention provides a gel staining and decoloring apparatus 100 including a container 110 and a fixing member; the container 110 is provided with a containing cavity 111, and the containing cavity 111 is used for containing dyeing liquid or decoloring liquid; a fixing assembly capable of being placed in the accommodating chamber 111, the fixing assembly including a first fixing plate 120, a second fixing plate 130, and a plurality of connecting blocks 140, at least two connecting blocks 140 being connected to opposite sides of the first fixing plate 130 in a horizontal direction, respectively; one end of the connecting block 140 is configured with two clamping portions 141 spaced along the vertical direction, i.e. the Z direction, the first fixing plate 120 and the second fixing plate 130 are correspondingly clamped in one clamping portion 141, and the first fixing plate 120 and the second fixing plate 130 are used for abutting against two opposite sides of the gel along the Z direction.

When the container 110 is used for dyeing operation, the container 110 is correspondingly filled with dyeing liquid; when the container 110 is used for decoloring, a decoloring solution is correspondingly contained in the container 110. Specifically, taking the connecting block 140 with two clamping portions 141 as an example, first clamping the second fixing plate 130 to one of the clamping portions of the connecting block 140, and after placing the gel, clamping the first fixing plate 120 to the other clamping portion of the connecting block 140, so that the two opposite sides of the gel abut against the first fixing plate 120 and the second fixing plate 130, respectively, thereby clamping and fixing the gel. After the gel is fixed between the two fixing plates, the entire fixing assembly is placed in the accommodating chamber 111 to perform a decoloring or dyeing operation. When the gel dyeing and decoloring device 100 is placed on a shaker for shaking, the gel is fixed in the clamping space formed by the first fixing plate 120 and the second fixing plate 130, so that the risk that the gel is turned over up and down and contacts the wall of the container 110 can be reduced, the possibility of gel breakage is reduced, and the subsequent experimental analysis effect is ensured. After dyeing or decoloration operation are accomplished, take out the whole chamber 111 that holds of fixed subassembly, then pull down first fixed plate 120 from connecting block 140, make the gel break away from the restriction of first fixed plate 120, incline second fixed plate 130 again, the side slope that second fixed plate 130 can be followed to the gel and go out, need not artifical manual glue of dragging for, reduce the injury of decoloration liquid and dyeing liquid to the human body, and can reduce the manual risk that leads to the colloid to break of dragging for glue, do benefit to the scanning of back facing gel and shoot.

Fig. 4 is a schematic view of the connection block 140 shown in fig. 3. As shown in fig. 3 and 4, in one embodiment, the clamping portion 141 may be a clamping groove recessed inwards along the Y direction, and when assembling, the first fixing plate 120 and the second fixing plate 130 respectively extend into the corresponding clamping groove to connect the two fixing plates and the connecting block 140, so that a clamping space is formed between the two fixing plates, and two opposite sides of the gel along the Z direction respectively abut against the two fixing plates, thereby fixing the gel. As shown in fig. 1, the plurality of connecting blocks 140 are uniformly distributed along the edge of the first fixing plate 120, so that the circumferential distance between the first fixing plate 120 and the second fixing plate 130 is consistent, the size of the clamping space formed between the first fixing plate 120 and the second fixing plate 130 is ensured to be constant, the position of the gel fixed between the two fixing plates is ensured not to be loosened, and the reliability of gel fixation is improved. Specifically, the recess depth of the card slot in the Y direction may be 1.2cm, and the gap between the two card slots, that is, the distance in the Z direction may be 1 cm. In other embodiments, the clamping portion may also be a protrusion extending outward along the Y direction, and the first fixing plate and the second fixing plate are supported on the protrusions at different positions, so as to connect the two fixing plates with the connecting block.

In still another embodiment, the clamping grooves are arranged at intervals in the Z direction of the connecting block, so that when the position of the second fixing plate is fixed, the size of the clamping space formed by the first fixing plate and the second fixing plate is changed by adjusting the first fixing plate to be connected with the clamping grooves at different positions, gels with different sizes can be fixed, and the use adaptability of the device is improved. It can be understood that the position of the first fixing plate can be fixed, and the second fixing plate is adjusted to be connected with the card slots at different positions; or the positions of the first fixing plate and the second fixing plate are not fixed, so that the position of the first fixing plate and the position of the second fixing plate can be adjusted, the first fixing plate and the second fixing plate are connected with clamping grooves at different positions, and the size of a clamping space is changed.

In one embodiment, the connecting block 140 is in clearance fit with the wall of the accommodating cavity 111, and when an external force acts on the first fixing plate 120 to move it in the Z direction, the connecting block 140 can slide against the wall of the accommodating cavity 111, thereby facilitating smooth placement and removal of the fixing assembly into and from the container 110. Moreover, because the connecting block 140 is attached to the wall of the accommodating cavity 111, when the device is placed on a shaking table or the solution is boiled in the heating process, the connecting block 140 cannot easily move due to a certain friction force between the connecting block 140 and the wall of the accommodating cavity 111, so that the risk of shaking the first fixing plate 120 and the second fixing plate 130 is reduced, and the fixing effect of the gel is ensured. The container 110 may be a rectangular parallelepiped, the length, width and height of the container 110 may be 12cm, 8cm and 8cm, respectively, and the wall thickness of the container 110 may be 2 mm. The two fixing plates are rectangular plates, and the overall length, width and height are slightly smaller than those of the first partial container 110, for example, the length, width and height may be 11.7cm, 7.7cm and 1cm, respectively. So that the first fixing plate 120 and the second fixing plate 130 can be just placed inside the container 110 and have a certain friction with the inner side of the container 110, reducing the risk of movement of the first fixing plate 120 and the second fixing plate 130.

Fig. 2 is a schematic view of the first fixing plate 120 shown in fig. 1. As shown in fig. 2, in one embodiment, a plurality of through holes 121 are formed on each of the first and second fixing plates 120 and 130. After the staining solution or the destaining solution is injected into the container 110, the liquid firstly flows to the gel through the through holes 121 on the first fixing plate 120 and then flows to the bottom of the container 110 through the through holes on the second fixing plate 130, so that the contact area between the gel and the liquid is increased, the gel is soaked, and the gel is ensured to be stained or destained uniformly. The shape of the through hole is not particularly limited, and may be a circular hole, a square hole, a stripe-shaped hole, or another polygonal hole. Specifically, taking the through holes as circular holes as an example, 96 through holes in 8 rows and 12 columns can be arranged on the two fixing plates, the diameter of each through hole can be 0.5mm, the interval between the adjacent through holes can be 0.2mm, and the gel is abutted to the solid area, namely the non-through hole area, on the fixing plate.

In an embodiment, the first fixing plate 120, the second fixing plate 130 and the container 110 are made of transparent materials such as quartz glass, so that an operator can observe the decoloring and dyeing conditions of the gel in real time, and the controllability is high. In addition, the device not only can realize the decoloration under the normal atmospheric temperature, dyeing operation, can also heat the liquid with the whole device of placing in the microwave oven, promotes the decoloration efficiency and the dyeing efficiency to the gel, and accommodation is wider. In other embodiments, the first fixing plate 120, the second fixing plate 130 and the container 110 may also be made of heat-resistant plastic or ceramic.

Further, handles 112 are disposed on two opposite sides of the container 110 along the X direction, and the container 110 can be transferred to other positions by holding the handles 112, so that the container 110 can be conveniently taken and placed.

As shown in fig. 1 and 2, in one embodiment, both ends of the first fixing plate 120 along the X direction are provided with pick-and-place slots 122 penetrating along the Z direction. In this way, an external tool may be inserted into the access slot 122 to clamp the first fixing plate 120, so as to place the fixing assembly into the container 110 or remove the fixing assembly from the container 110. The length of the pick-and-place slot 122 in the Y direction may be 2 cm.

Fig. 5 is a schematic view of a clamping member 160 for clamping the first fixing plate. As shown in fig. 5, in one embodiment, the gel dyeing and decoloring apparatus 100 further includes a clamping member 160, the clamping member 160 extends outward along two sides of its width direction to form protruding portions 161, the clamping member 160 has certain elasticity and can be opened at a certain angle, so that the protruding portions 161 at two sides can respectively protrude into two access slots 122 distributed along the X direction, and the protruding portions 161 can abut against the bottom wall of the first fixing plate 120, thereby lifting the fixing assembly upwards or downwards into the container 110. The extension length of the protruding portion 161 in the X direction may be 5mm, so as to ensure a sufficient contact area with the first fixing plate 120, and ensure the stability and reliability of the clamping.

As shown in fig. 1, in one embodiment, a plurality of positioning pillars 113 extend upward from the bottom wall of the accommodating cavity 111, and when the fixing assembly is placed in the container 110, the positioning pillars 113 can function to position and support the second fixing plate 130, so that the second fixing plate 130 and the bottom wall of the container 110 have a certain gap, which can ensure sufficient soaking of the fixing assembly. The positioning pillars 113 may be specifically disposed in four corner regions of the container 110, and the positioning pillars 113 may have a rectangular parallelepiped shape, and the length, width, and height of the positioning pillars 113 may be 5mm, and 1.5cm, respectively.

Fig. 6 is a schematic view of the container 110 and the cap 150 shown in fig. 1. As shown in fig. 1 and 6, in one embodiment, the gel dyeing and decoloring apparatus 100 further includes a cover 150 disposed on the top of the container 110, the cover 150 is provided with a handle 151, and when the cover 150 is placed on the top of the container 110 by the handle 151, the accommodating cavity 111 is closed by the cover 150, so that the apparatus has a certain tightness, and the pollution of volatile, irritant and toxic gases in the liquid to the laboratory is reduced. When the dyeing or decoloring is completed, the cover 150 may be removed by the handle 151, thereby taking out the fixed components inside the container 110.

Further, the lateral wall that container 110 is close to the bottom is provided with the leakage fluid dram, and the leakage fluid dram is provided with the shutoff and blocks up, and when taking off the shutoff stopper back, can realize the automatic of dyeing liquor, decoloration liquid through this leakage fluid dram and discharge, reduce experiment operating personnel and liquid direct contact's chance. And after the liquid drainage is finished, plugging the liquid drainage port by using a plugging plug.

As shown in fig. 1, taking the device to perform the normal temperature dyeing operation as an example, the second fixing plate 130 is firstly clamped to the connecting block 140, the protein gel after electrophoresis is placed on the second fixing plate 130, then the first fixing plate 120 is covered, and the first fixing plate 120 is clamped to the connecting block 140. The protrusion 161 of the clamping member 160 protrudes into the access slot 122, and the fixing assembly is placed into the container 110, and then the clamping member 160 is taken out. Coomassie brilliant blue staining solution is added, the staining solution is submerged in the first fixing plate 120 for at least 3cm, then the cover body 150 is covered, and horizontal shaking staining is carried out for 30min at room temperature. After dyeing is finished, the liquid outlet is opened, and the dyeing liquid is discharged. And then plugging the liquid outlet, removing the cover body 150, injecting a decoloring solution, and decoloring for 2 hours. And after the decolorization is finished, discharging the decolorized liquid through a liquid outlet. Injecting clear water into the container 110 to clean the gel, removing the residual decolorizing reagent, finally taking out the whole fixing assembly by using the clamping piece 160, then taking out the first fixing plate 120 from the connecting block 140, inclining the second fixing plate 130, enabling the gel to be inclined out from the side edge of the second fixing plate 130, and then performing subsequent imaging analysis by using a gel imager. When heating is performed by a microwave oven, the dyeing time and the decoloring time can be correspondingly shortened to half.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the utility model. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims (10)

1. The gel dyeing and decoloring device is characterized by comprising a container and a fixing component;

the container is provided with a containing cavity, and the containing cavity is used for containing dyeing liquid or decoloring liquid;

the fixing assembly can be placed in the accommodating cavity and comprises a first fixing plate, a second fixing plate and a plurality of connecting blocks, and at least two connecting blocks are respectively connected to two opposite sides of the first fixing plate in the horizontal direction; the one end of connecting block is constructed two joint portions along vertical direction interval, first fixed plate with the second fixed plate corresponds the joint respectively in one joint portion, first fixed plate with the second fixed plate is used for following with the gel the relative both sides butt of vertical direction.

2. The gel dyeing and decoloring device according to claim 1, wherein the clamping portion comprises a clamping groove recessed in a horizontal direction, and the first fixing plate and the second fixing plate are respectively clamped with the corresponding clamping grooves.

3. The gel dyeing and decoloring device according to claim 2, wherein at least three slots are formed in the vertical direction of the connecting block at intervals, and the first fixing plate and/or the second fixing plate can be connected to the slots at different positions.

4. The gel staining and decoloring device according to claim 1, wherein the fixing component is in clearance fit with the wall of the accommodating chamber, and the connecting block is capable of sliding along the wall of the accommodating chamber.

5. The apparatus for dyeing and decoloring gel according to claim 1, wherein a plurality of positioning posts extend upward from a bottom wall of the receiving chamber, and the positioning posts are used for supporting the second fixing plate.

6. The gel staining and destaining apparatus of claim 1, wherein the first and second retaining plates are each configured with a plurality of through holes therein.

7. The gel staining and destaining apparatus of claim 1, further comprising a cover removably attached to a top of the container, the cover for closing the containment chamber.

8. The apparatus for dyeing and decoloring gel according to claim 1, wherein both ends of the first fixing plate in the horizontal direction are provided with pick-and-place slots penetrating in the vertical direction.

9. The gel staining and decoloring device of claim 8, further comprising a clamping member having an insertion portion that can pass through the access slot and abut against the bottom wall of the first fixing plate.

10. The apparatus for dyeing and decoloring gel according to claim 1, wherein said first fixing plate, said second fixing plate and said container are made of a transparent material.

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