CN216320477U

CN216320477U - Novel organoid chromatographic separation device

Info

Publication number: CN216320477U
Application number: CN202122690947.8U
Authority: CN
Inventors: 徐长城; 陈荪睿
Original assignee: Shanghai Wanheyuan Biotechnology Co ltd
Current assignee: Shanghai Wanheyuan Biotechnology Co ltd
Priority date: 2021-11-05
Filing date: 2021-11-05
Publication date: 2022-04-19
Anticipated expiration: 2031-11-05

Abstract

The utility model discloses a novel organoid chromatographic separation device, which belongs to the technical field of chromatographic devices and comprises a top pipe, a middle pipe and a bottom pipe, wherein the top pipe is in threaded connection with the middle pipe, the middle pipe is in threaded connection with the bottom pipe, a filter plate is arranged inside the top pipe and used for filtering organoid cells larger than cubic millimeters, and a filter membrane is arranged inside the middle pipe and used for absorbing organoid cells of cubic millimeters. Through setting up well pipe, top pipe and bottom tube, the cellular fluid that will separate carries out the subregion and handles for the cellular fluid that reachs at last can not be thoughtlessly there is not the cellular fluid that covers scope size of volume, and final treatment is better.

Description

Novel organoid chromatographic separation device

Technical Field

The utility model belongs to the technical field of chromatographic devices, and particularly relates to a novel organoid chromatographic separation device.

Background

Chromatography is a technique established by utilizing the difference in physicochemical properties of different substances. All chromatography systems consist of two phases: one is a stationary phase and the other is a mobile phase. When the mixture to be separated passes through the stationary phase with the mobile phase, the distribution (content ratio) in the two phases is different due to the difference in the physicochemical properties of the components, the ability to interact (adsorb, dissolve, bind, etc.) with the two phases, and the components are constantly redistributed in the two phases as the mobile phase moves forward. The effluent liquid is collected in a fractional way, so that each single component contained in the sample can be obtained, and the aim of separating each component is fulfilled.

When the organoid tissue is separated by using a chromatographic separation technology, the organoid tissue can be separated into a plurality of layers according to the size of the tissue volume, so that the tissues with different sizes can be conveniently tested, but the cell sap of the plurality of layers are in the same test tube during layering, and the cell sap is inconvenient to take out. To this end, we propose a novel organoid chromatographic separation device to solve the above problems.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a novel organoid chromatographic separation device, which solves the problem that the cell sap is difficult to take out because several layers of cell sap are in the same test tube during layering in the use process of the conventional organoid chromatographic separation device in the background technology.

In order to achieve the purpose, the utility model provides the following technical scheme: the utility model provides a novel organoid chromatography separator, includes push pipe, well pipe and bottom tube, push pipe and well pipe threaded connection, well pipe and bottom tube threaded connection, the internally mounted of push pipe has the filter plate, the filter plate is used for filtering the organoid cell that is greater than cubic millimeter, the internally mounted of well pipe has the filter membrane, the filter membrane is used for absorbing-cubic millimeter's organoid cell.

Preferably, the filter plate is positioned at the joint of the top pipe and the middle pipe, and the filter membrane is positioned at the joint of the middle pipe and the bottom pipe.

Preferably, the interior screw thread of well pipe is installed and is accepted the board, the filter membrane is placed on the surface of accepting the board.

Preferably, the lower surface of the bearing plate is fixedly connected with a guide plate, and a rotating rod is arranged in the guide plate.

Preferably, the surfaces of the middle pipe and the bottom pipe are both provided with outlet pipes, and the inlet ends of the outlet pipes are lower than the connecting lines between the middle pipe and the bottom pipe and between the middle pipe and the top pipe.

Preferably, the surface of the top pipe is in threaded connection with the inner wall of the middle pipe, and the surface of the middle pipe is in threaded connection with the inner wall of the bottom pipe.

Preferably, the inner walls of the middle pipe and the bottom pipe are both in threaded connection with sealing plates.

Preferably, the surface of the bearing plate is in a corrugated shape.

Preferably, a flow guide pipe is arranged inside the top pipe, the upper part of the flow guide pipe is funnel-shaped, and the lower part of the flow guide pipe is in a straight pipe shape and is located in the center of the top pipe.

Preferably, the funnel-shaped maximum radius of the guide pipe is larger than the inner diameter of the top pipe, and the minimum radius of the fox-searching guide pipe is smaller than the inner diameter of the top pipe.

Compared with the prior art, the utility model has the beneficial effects that:

1. through setting up well pipe, top pipe and bottom tube, the cellular fluid that will separate carries out the subregion and handles for the cellular fluid that reachs at last can not be thoughtlessly there is not the cellular fluid that covers scope size, and final treatment is better.

2. Accept board, bull stick and guide plate through the setting, prevent that cell sap from at the in-process that flows, flowing to the surface of bull stick, be infected with in hand, utilize simultaneously to accept the board and install the filter membrane, can not destroy the surface of filter membrane, reuse bull stick will accept the board and take out convenient operation is swift.

3. The cell sap is poured out by using the outlet pipe, so that the cell sap is prevented from being accumulated at the thread position of the joint, and unnecessary waste is avoided.

Drawings

FIG. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a schematic view of the structure at the outlet pipe of the present invention;

FIG. 3 is a schematic diagram of the structure of a filter membrane according to the present invention;

FIG. 4 is a schematic sectional view of the filter according to the present invention;

FIG. 5 is a schematic view of the receiving plate of the present invention;

fig. 6 is a schematic structural view of the draft tube of the present invention.

In the figure: 1. jacking pipes; 2. a middle tube; 3. a bottom tube; 4. filtering the plate; 5. filtering the membrane; 6. a bearing plate; 7. a baffle; 8. a rotating rod; 9. an outlet pipe; 10. a sealing plate; 11. and a flow guide pipe.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-6, the present invention provides a technical solution: the utility model provides a novel organoid chromatography separator, includes push pipe 1, well pipe 2 and bottom tube 3, push pipe 1 and well 2 threaded connection of pipe,

well pipe

2 and 3 threaded connection of bottom tube, the internally mounted of push pipe 1 has filter plate 4, filter plate 4 is used for filtering the organoid cell that is greater than 50 cubic millimeters, the internally mounted of well pipe 2 has filter membrane 5, filter membrane 5 is used for absorbing 40-50 cubic millimeters's organoid cell.

In the embodiment, the top tube 1, the middle tube 2 and the bottom tube 3 are separated, so that the separated cell sap can be conveniently separated and cleaned.

Specifically, the filter plate 4 is positioned at the joint of the top pipe 1 and the middle pipe 2, and the filter membrane 5 is positioned at the joint of the middle pipe 2 and the bottom pipe 3.

In this embodiment, the inner parts of the top pipe 1 and the middle pipe 2 can receive more cell sap.

Specifically, the inner thread of the middle pipe 2 is provided with a bearing plate 6, and the filter membrane 5 is placed on the surface of the bearing plate 6.

In this embodiment, the filter membrane 5 is easily mounted and fixed.

Specifically, a guide plate 7 is fixedly connected to the lower surface of the bearing plate 6, and a rotating rod 8 is installed inside the guide plate 7.

In this embodiment, the receiving plate 6 is rotated by the rotating rod 8, and the cell sap is guided by the guide plate 7 to prevent the cell sap from being stained on the surface of the rotating rod 8.

Specifically, the surfaces of the middle pipe 2 and the bottom pipe 3 are both provided with an outlet pipe 9, and the inlet end of the outlet pipe 9 is lower than the connecting lines between the middle pipe 2 and the bottom pipe 3 and between the middle pipe 2 and the top pipe 1.

In the embodiment, when the cell sap is poured out, the cell sap is prevented from being accumulated at the connection part of the screw threads, so that the cell sap is prevented from being wasted.

Specifically, the surface of the top tube 1 is in threaded connection with the inner wall of the middle tube 2, and the surface of the middle tube 2 is in threaded connection with the inner wall of the bottom tube 3.

In this embodiment, the cell sap is introduced from top to bottom, and the cell sap on the surface is contaminated on the top walls of the middle tube 2 and the bottom tube 3, resulting in waste of the cell sap.

Specifically, the inner walls of the middle tube 2 and the bottom tube 3 are both in threaded connection with a sealing plate 10.

In this embodiment, the surfaces of the middle tube 2 and the bottom tube 3 are sealed with a sealing plate 10 when the cell sap is poured out.

Specifically, the surface of the receiving plate 6 is in a corrugated shape.

In this embodiment, the cell fluid is allowed to flow down from the surface of the filter membrane 5 and the gap of the receiving plate 6.

Specifically, the inside of push pipe 1 is installed with honeycomb duct 11, honeycomb duct 11's upper portion is for leaking hopper-shaped, honeycomb duct 11's lower part is for straight tubulose and be located push pipe 1's midpoint.

In this embodiment, the flow guide tube 11 is placed inside the top tube 1, and the cell sap directly falls into the top tube 1 through the flow guide tube 11 without hanging on the inner wall of the top tube 1.

Specifically, the maximum funnel-shaped radius of the flow guide tube 11 is larger than the inner diameter of the top tube 1, and the minimum radius of the flow guide tube 11 is smaller than the inner diameter of the top tube 1.

In this embodiment, the flow guide tube 11 is conveniently placed on the top tube 1 and is also conveniently taken down.

The working principle and the using process of the utility model are as follows: cell sap is led out to the interior of the top pipe 1 through the guide pipe 11, the cell sap larger than 50 cubic millimeters is filtered through the filter plate 4 and flows into the interior of the middle pipe 2 to obtain the cell sap with the volume smaller than 50, the cell sap larger than 40 and smaller than 50 is absorbed by the filter membrane 5, finally the cell sap smaller than 40 cubic millimeters is contained in the bottom pipe 3, finally the cell sap is detached, the middle pipe 2 and the bottom pipe 3 are sealed through the sealing plate 10, one of the cell sap is poured out from the outlet pipe 9, the bearing plate 6 is taken out by rotating the rotating rod 8, the filter membrane 5 is taken out, and other liquids are used for separation.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a novel organoid chromatographic fractionation device which characterized in that: including push pipe (1), well pipe (2) and bottom tube (3), push pipe (1) and well pipe (2) threaded connection, well pipe (2) and bottom tube (3) threaded connection, the internally mounted of push pipe (1) has filter plate (4), filter plate (4) are used for filtering the organoid cell that is greater than 50 cubic millimeters, the internally mounted of well pipe (2) has filter membrane (5), filter membrane (5) are used for absorbing the organoid cell of 40-50 cubic millimeters.

2. The novel organoid chromatographic separation device of claim 1, wherein: the filter plate (4) is positioned at the joint of the top pipe (1) and the middle pipe (2), and the filter membrane (5) is positioned at the joint of the middle pipe (2) and the bottom pipe (3).

3. The novel organoid chromatographic separation device of claim 1, wherein: the internal thread of the middle pipe (2) is provided with a bearing plate (6), and the filter membrane (5) is placed on the surface of the bearing plate (6).

4. The novel organoid chromatographic separation device of claim 3, wherein: the lower surface of the bearing plate (6) is fixedly connected with a guide plate (7), and a rotating rod (8) is arranged inside the guide plate (7).

5. The novel organoid chromatographic separation device of claim 1, wherein: the surface of well pipe (2) and bottom tube (3) all installs outlet pipe (9), the entry end of outlet pipe (9) is less than well pipe (2) and bottom tube (3), well pipe (2) and top pipe (1) between connecting line department.

6. The novel organoid chromatographic separation device of claim 1, wherein: the surface of the top pipe (1) is in threaded connection with the inner wall of the middle pipe (2), and the surface of the middle pipe (2) is in threaded connection with the inner wall of the bottom pipe (3).

7. The novel organoid chromatographic separation device of claim 1, wherein: the inner walls of the middle pipe (2) and the bottom pipe (3) are both in threaded connection with a sealing plate (10).

8. The novel organoid chromatographic separation device of claim 3, wherein: the surface of the bearing plate (6) is in a check pattern shape.

9. The novel organoid chromatographic separation device of claim 1, wherein: the internally mounted of push pipe (1) has honeycomb duct (11), the upper portion of honeycomb duct (11) is for leaking hopper-shaped, the lower part of honeycomb duct (11) is for straight tubulose and be located the centre of push pipe (1).

10. The novel organoid chromatographic separation device of claim 9, wherein: the maximum funnel-shaped radius of the flow guide pipe (11) is larger than the inner diameter of the top pipe (1), and the minimum radius of the flow guide pipe (11) is smaller than the inner diameter of the top pipe (1).