CN216274182U - Up-down magnetic stirring system - Google Patents
Up-down magnetic stirring system Download PDFInfo
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- CN216274182U CN216274182U CN202122386221.5U CN202122386221U CN216274182U CN 216274182 U CN216274182 U CN 216274182U CN 202122386221 U CN202122386221 U CN 202122386221U CN 216274182 U CN216274182 U CN 216274182U
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- steel shell
- stirring
- magnetic
- biological reaction
- reaction bag
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Abstract
The utility model discloses an up-and-down magnetic stirring system which comprises a steel shell and a magnetic stirrer arranged on the steel shell, wherein the steel shell is arranged on a rack and can rotate up and down relative to the rack, the bottom and the top of the steel shell are respectively provided with the magnetic stirrer, and the two magnetic stirrers share one stirring shaft. The utility model can improve the stability of the stirring shaft and the stirring blades on the stirring shaft in the oversized reaction bag, is convenient to add a plurality of layers of stirring blades on the stirring shaft, provides better mass transfer effect, and solves the contradiction between large-volume reaction bags and poor stirring mass transfer effect.
Description
Technical Field
The utility model relates to the technical field of biological pharmacy, in particular to stirring equipment.
Background
Patent document CN 112980678A discloses a cylindrical coupling overhead magnetic stirrer for disposable bioreactor bags. It comprises an inner rotor, an outer rotor, an isolation sleeve and a stirring shaft. The isolation sleeve is sleeved outside the inner rotor, the outer rotor is sleeved outside the isolation sleeve, the inner magnetic steel is arranged inside the inner rotor, the outer magnetic steel is arranged inside the outer rotor, and the outer rotor and the inner rotor are isolated through the isolation sleeve. The upper part of the stirring shaft penetrates through the center of the inner rotor and is arranged in the isolation sleeve through a bearing, and the lower part of the stirring shaft is provided with stirring blades. The motor output shaft of the stirring driving motor is connected with the top end of the outer rotor. The stirring driving motor is installed on the motor fixing beam, the isolation sleeve is installed on the lower cross arm, the bottom of the isolation sleeve is connected with the disposable biological reaction bag, an opening used for extending into the stirring shaft is sealed, and the disposable biological reaction bag is placed in the steel shell.
The above patent document describes a cylindrical surface coupling overhead magnetic stirrer (hereinafter referred to as overhead magnetic stirrer) for a disposable bioreactor bag, which is arranged at the top of the disposable bioreactor bag as the name implies, wherein the stirring shaft extends downwards into the disposable bioreactor bag, and the bottom of the stirring shaft is unsupported and is in a suspended state. For a large disposable biological reaction bag, the overhead magnetic stirrer described in the above patent document has insufficient mass transfer effect for stirring at the bottom of the disposable biological reaction bag, the upper part of the multi-layer stirring blade is unstable, and the stirring shaft is easy to shake.
SUMMERY OF THE UTILITY MODEL
The technical problems solved by the utility model are as follows: improve the mass transfer effect in the large-volume reaction bag.
In order to solve the technical problems, the utility model provides the following technical scheme: the utility model provides a magnetic stirring system from top to bottom, is including installing box hat in the frame, setting up the magnetic stirrers on the box hat, and the bottom and the top of box hat are equipped with magnetic stirrers respectively, and a (mixing) shaft of two magnetic stirrers shares, and the (mixing) shaft sets up in the disposable biological reaction bag in the box hat.
The upper magnetic stirrer and the lower magnetic stirrer act synchronously to drive the same stirring shaft to rotate, and the stirring paddle on the stirring shaft stirs the liquid in the disposable biological reaction bag.
The utility model has the advantages of improving the stability of the stirring shaft and the stirring blades on the stirring shaft in the oversized reaction bag, facilitating the addition of a plurality of layers of stirring blades on the stirring shaft, providing better mass transfer effect and solving the contradiction between large-volume reaction bags and poor stirring mass transfer effect.
Drawings
The utility model is further described below with reference to the accompanying drawings:
FIG. 1 is a schematic view of an up-down magnetic stirring system;
FIG. 2 is a schematic view of the top view of FIG. 1 showing a pair of clamping blocks 13 mounted in a top slot structure 102 of the top of the steel can 10;
FIG. 3 is a schematic view of the pair of clamping blocks 13 shown in FIG. 2 after being hidden;
FIG. 4 is a schematic view of cross-arm 123 of FIG. 1;
FIG. 5 is a top view of FIG. 4;
FIG. 6 shows shear rate(s) in a CFD simulation study of a prior art 6T stirred bioreactor-1) A highest region distribution map;
FIG. 7 shows the energy dissipation ratio (m) in a CFD simulation study of a 6T stirred bioreactor of the prior art2/s3) The highest region distribution map.
The symbols in the drawings illustrate that:
10. a steel shell; 101. an inner edge; 102. a slot structure; 103. perforating; 11. a frame; 12. a first bracket; 121. a support; 122. erecting an arm; 123. a cross arm; 124. a strip-shaped groove; 125. a slider; 126. a motor mounting seat; 127. a fastener; 13. a clamping block; 130. a screw; 15. a second bracket; 16. a lifting cylinder; 160. a connecting member;
20. a disposable biological reaction bag; 21. a stirring shaft; 210. a first type of stirring blade; 22. an inner rotor; 220. a second type of stirring blade; 221. an inner rotor housing; 223. an inner rotor magnet; 23. a top seal structure; 230. a limiting groove; 231. the top outer rotor is matched with the groove; 24. a bottom sealing structure; 241. the bottom outer rotor is matched with the groove;
301. a first stirrer; 302. a second agitator;
31. a stirring driving motor; 32. an outer rotor;
20', a reaction bag of the prior art equipped with a single magnetic stirrer; 21', a stirring shaft driven by a single magnetic stirrer in the prior art; 210' of the prior art.
Detailed Description
Referring to fig. 1, an up-down magnetic stirring system comprises a steel shell 10 mounted on a frame 11, and magnetic stirrers arranged on the steel shell, wherein the magnetic stirrers are respectively arranged at the bottom and the top of the steel shell, the two magnetic stirrers share a stirring shaft 21, and the stirring shaft is arranged in a disposable biological reaction bag 20 in the steel shell.
The stirring driving motors of the two magnetic stirrers are connected with a power supply through wires, for example, the two stirring driving motors are connected in series in the same circuit, the circuit is provided with a switch, the switch is closed, the power supply supplies power to the two stirring driving motors simultaneously, the two same stirring driving motors act simultaneously to drive the same stirring shaft to rotate, and the stirring blades on the stirring shaft stir the liquid in the disposable biological reaction bag 30.
Either magnetic stirrer comprises a stirring drive motor 31 and an outer rotor 32. The disposable bioreactor bag 20 has an inner rotor 22 and a sealing structure at the top and bottom thereof, and the sealing structure is hermetically connected to the disposable bioreactor bag. The sealing structure at the top of the disposable bioreactor bag is set as the top sealing structure 23, and the sealing structure at the bottom of the disposable bioreactor bag is set as the bottom sealing structure 24. The inner rotor is positioned in the disposable biological reaction bag, two ends of the stirring shaft 21 are respectively connected with the inner rotor, and the inner rotor is pivoted with the sealing structure. Any inner rotor comprises an inner rotor shell 221 and an inner rotor magnet 223 sealed in the inner rotor shell, the top end of the stirring shaft 21 is fixedly connected with the inner rotor at the top of the disposable biological reaction bag 20, the bottom end of the stirring shaft is fixedly connected with the inner rotor at the bottom of the disposable biological reaction bag, and the inner rotor 22 is pivoted on the sealing structure through a bearing. The top sealing structure 23 is provided with a top outer rotor matching groove 231, the bottom sealing structure 24 is provided with a bottom outer rotor matching groove 241, and the top outer rotor matching groove and the bottom outer rotor matching groove are positioned outside the disposable bioreactor bag 20. In practice, the outer rotor of the magnetic stirrer at the top of the steel shell fits into the top outer rotor fitting groove 231, and the outer rotor of the magnetic stirrer at the bottom of the steel shell fits into the bottom outer rotor fitting groove 241. Any outer rotor is provided with a magnet, and under the drive of the stirring drive motor 31, the outer rotor rotates in the corresponding outer rotor matching groove to drive the corresponding inner rotor 22 to rotate, and the inner rotor drives the stirring shaft 21 to rotate.
The stirring shaft 21 is provided with a first type stirring blade 210, the inner rotor at the bottom of the disposable biological reaction bag 20 is provided with a second type stirring blade 220, and substances in the disposable biological reaction bag are easy to sink under the action of gravity, so that the second type stirring blade can stir and lift the sinking substances to be fully mixed with other substances in the disposable biological reaction bag.
The magnetic stirrers at top of box hat 10 is first agitator 301, and the upper portion of box hat is equipped with the first support 12 that supports the stirring driving motor of first agitator, combines fig. 1 to 3, and the top of box hat is equipped with inward flange 101, is equipped with draw-in groove structure 102 on the inward flange, and a pair of clamp splice 13 cooperation is in the draw-in groove structure, and the seal structure at disposable biological reaction bag top can be held to a pair of clamp splice, and the seal structure at disposable biological reaction bag top is equipped with spacing groove 230. The disposable biological reaction bag is placed into the steel shell 10 from the top opening of the steel shell, the disposable biological reaction bag is flexible and can deform randomly, the position of the top sealing structure 23 is unstable, and the outer rotor at the top is difficult to be matched with the top sealing structure. To this end, a pair of clamping blocks 13 are fitted into the catching groove structure 103 at their sides close to each other, and the pair of clamping blocks are formed in a disk shape having a through hole at the center thereof, which is fitted into the catching groove 230. In this manner, the top seal 23 is secured by the pair of clamp blocks 13. The operator mounts a screw 130 on the clamp 13 to secure the clamp to the inner edge 101.
Referring to fig. 1, 4 and 5, the first support 12 includes a base 121 mounted on the steel shell 10, a vertical arm 122 pivotally connected to the base, and a crossbar 123 connected to the vertical arm, the crossbar being capable of being raised and lowered along the vertical arm, and a stirring driving motor of the first stirrer 301 being mounted on the crossbar. In practice, the vertical arms 122 and the horizontal arms 123 are located beside the top opening of the steel shell before the disposable bioreactor bag 20 is placed in the steel shell 10. After the disposable bioreactor bag is placed in the steel shell, the operator rotates the vertical arm 122 to make the cross arm 123 and the stirring driving motor thereon located right above the top sealing structure 23. With the top seal structure 23 secured by the pair of clamp blocks 13, the crossbar 123 descends and the outer rotor of the first agitator 301 descends to fit into the top outer rotor fitting groove 231.
As shown in fig. 1, the vertical arm 122 is provided with a strip-shaped groove 124, a sliding block 125 is fitted in the strip-shaped groove, the sliding block is connected with a cross arm 123, and one end of the cross arm is provided with a motor mounting seat 126. The agitation drive motor of the first agitator 301 is mounted on the motor mount 126. The slider 125 is screwed with a fastener 127, and after the outer rotor of the first agitator is matched with the top outer rotor matching groove 231, an operator screws the fastener, and the fastener can abut against the inner wall of the strip-shaped groove 124, so that the slider is fixed in position, and further, the cross arm is fixed in position on the vertical arm 122.
The magnetic stirrer at the bottom of the steel shell 10 is a second stirrer 302; the bottom of the steel shell is provided with a second support 15 for supporting a stirring driving motor of a second stirrer, the second support is arranged on a lifting cylinder 16, and the lifting cylinder is connected to the steel shell through a connecting piece 160. The disposable biological reaction bag 20 is put into the steel shell, and the outer rotor on the shaft of the stirring driving motor of the second stirrer rises under the driving of the lifting cylinder, passes through the bottom of the steel shell and is matched with the bottom outer rotor matching groove 241 of the bottom sealing structure. The lifting cylinder 16 facilitates the detachment and attraction of the outer rotor and the inner rotor at the bottom.
The bottom of the steel can 10 is provided with a perforation 103 in which a sealing structure of the bottom of the disposable bioreactor bag 20 is fitted. In this manner, bottom seal structure 24 is positioned in said through hole 103, providing conditions for the outer rotor to mate with bottom outer rotor mating groove 241, and for stirring shaft 21 to stand upright. Wherein, the vertical state of the stirring shaft provides conditions for the smooth rotation of the stirring shaft to stir the substances in the disposable biological reaction bag 20.
Shear rate(s) according to the CFD simulation study of a 6T stirred bioreactor-1) Highest zone value9.286-10.000, the distribution of the areas with the highest shear rate is shown as the shaded portion in FIG. 6.
Energy dissipation ratio (m) according to CFD simulation study of 6T stirred bioreactor2/s3) The highest zone value is 0.139-0.150, and the distribution of the highest energy dissipation rate zone is shown as the shaded part in fig. 7.
Fig. 6 and 7 show the working condition of the stirring shaft driven by a single magnetic stirrer. As can be seen from fig. 6, in the region of highest shear rate, the stirring blade close to the magnetic stirrer has a higher shear rate. As can be seen from fig. 7, in the region where the energy dissipation ratio is highest, the stirring blade close to the magnetic stirrer has a lower energy dissipation ratio. According to the utility model, the magnetic stirrers are respectively arranged at the upper end and the lower end of one stirring shaft, so that the shear rate of the stirring blades can be effectively improved, and the energy dissipation rate of the stirring blades is reduced.
A method for installing an up-and-down magnetic stirring system comprises the following steps.
Firstly, a disposable biological reaction bag 20 is placed into the steel shell 10 from the top opening of the steel shell, and a sealing structure at the bottom of the disposable biological reaction bag is matched in a through hole 103 at the bottom of the steel shell; the stirring shaft 21 is upright.
Second, the elevating cylinder 16 installed at the bottom of the steel can 10 drives the stirring driving motor of the second stirrer 302 to ascend, and the outer rotor on the stirring driving motor shaft is fitted into the bottom outer rotor fitting groove 241 of the bottom sealing structure 24.
Thirdly, a pair of clamping blocks 13 are matched in the clamping groove structure 102 at the top of the steel shell 10, the clamping blocks are folded oppositely, and the clamping blocks clamp the sealing structure at the top of the disposable biological reaction bag 20. Specifically, the pair of clamping blocks 13 clamp the stopper groove 230 of the top seal structure 23.
Fourthly, the vertical arm 122 rotates with respect to the support 121 of the steel shell 10, the horizontal arm 123 of the vertical arm rotates with the vertical arm, the stirring driving motor of the first stirrer 301 of the horizontal arm goes above the sealing structure at the top of the disposable bioreactor bag 20, the horizontal arm descends along the vertical arm, and the outer rotor of the stirring driving motor shaft of the first stirrer is fitted into the top outer rotor fitting groove 231 of the top sealing structure 23.
The above description is only a preferred embodiment of the present invention, and for those skilled in the art, the present invention should not be limited by the description herein, since various changes and modifications can be made in the details of the embodiment and the application range according to the spirit of the present invention.
Claims (6)
1. The utility model provides an upper and lower magnetic stirring system, includes install box hat (10) on frame (11), sets up the magnetic stirrers on the box hat, its characterized in that: the bottom and the top of the steel shell are respectively provided with a magnetic stirrer, the two magnetic stirrers share a stirring shaft (21), and the stirring shaft is arranged in a disposable biological reaction bag (20) in the steel shell.
2. The up-down magnetic stirring system as recited in claim 1, further comprising: any magnetic stirrer comprises a stirring driving motor (31) and an outer rotor (32); the top and the bottom of the disposable biological reaction bag (20) are both provided with an inner rotor (22) and a sealing structure, the sealing structure is connected with the disposable biological reaction bag in a sealing way, the inner rotor is positioned in the disposable biological reaction bag, the two ends of the stirring shaft (21) are respectively connected with the inner rotor, the inner rotor is pivoted with the sealing structure, the sealing structure is provided with an outer rotor matching groove, and the outer rotor matching groove is positioned outside the disposable biological reaction bag.
3. A vertical magnetic stirring system as set forth in claim 2 wherein: the magnetic stirrers at top of steel shell (10) is first agitator (301), the upper portion of steel shell is equipped with first support (12) that support the stirring driving motor of first agitator, the top of steel shell is equipped with inward flange (101), be equipped with draw-in groove structure (102) on the inward flange, a pair of clamp splice (13) cooperation is in the draw-in groove structure, the seal structure at disposable biological reaction bag top can be held to a pair of clamp splice, the seal structure at disposable biological reaction bag top is equipped with spacing groove (230).
4. A vertical magnetic stirring system as set forth in claim 3 wherein: the first support (12) comprises a support (121) arranged on the steel shell (10), a vertical arm (122) pivoted on the support and a cross arm (123) connected to the vertical arm, wherein the cross arm can lift along the vertical arm, and a stirring driving motor of the first stirrer (301) is arranged on the cross arm.
5. A vertical magnetic stirring system as set forth in claim 2 wherein: the magnetic stirrer at the bottom of the steel shell (10) is a second stirrer (302); and a second support (15) for supporting a stirring driving motor of a second stirrer is arranged at the bottom of the steel shell, the second support is arranged on a lifting cylinder (16), and the lifting cylinder is connected to the steel shell.
6. An up-down magnetic stirring system as set forth in claim 5 wherein: the bottom of the steel shell (10) is provided with a perforation (103), and a sealing structure at the bottom of the disposable biological reaction bag (20) is matched in the perforation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122386221.5U CN216274182U (en) | 2021-09-30 | 2021-09-30 | Up-down magnetic stirring system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122386221.5U CN216274182U (en) | 2021-09-30 | 2021-09-30 | Up-down magnetic stirring system |
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| Publication Number | Publication Date |
|---|---|
| CN216274182U true CN216274182U (en) | 2022-04-12 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202122386221.5U Active CN216274182U (en) | 2021-09-30 | 2021-09-30 | Up-down magnetic stirring system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114768639A (en) * | 2022-04-29 | 2022-07-22 | 百林科制药装备科技(江苏)有限公司 | Stirring device |
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2021
- 2021-09-30 CN CN202122386221.5U patent/CN216274182U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114768639A (en) * | 2022-04-29 | 2022-07-22 | 百林科制药装备科技(江苏)有限公司 | Stirring device |
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