CN216274173U

CN216274173U - Oxygenation and mixing device of low-shear-force airlift fermentation tank

Info

Publication number: CN216274173U
Application number: CN202120554944.9U
Authority: CN
Inventors: 吕和鑫; 贾士儒; 刘新; 张有为; 张丽娜
Original assignee: Tianjin University of Science and Technology
Current assignee: Tianjin University of Science and Technology
Priority date: 2021-03-18
Filing date: 2021-03-18
Publication date: 2022-04-12
Anticipated expiration: 2031-03-18

Abstract

The utility model provides a low-shear force airlift type fermentation tank oxygenation and mixing device. According to the technical scheme, besides the air inlet distributor, a plurality of wheels of air stones are additionally arranged in the airlift fermentation tank, and the air stones and the nozzle direction of the air guide cup can be adjusted to adjust the gas-liquid flow along the circulation direction of the guide cylinder. After the micro bubble flow is sprayed into the fermentation tank, the formed gas-liquid flow circularly flows under the guidance of the guide cylinder, and the uniform mixing of the fermentation liquid and the thalli is realized. The air distributor is arranged at a discharge port at the bottom of the tank and comprises a bottom air stone and a metal cover plate. The dissolved oxygen concentration of the fermentation tank is efficiently increased by the nano-to micron-diameter bubbles sprayed by the air stones, and the dissolved oxygen level of the fermentation liquor is controlled by adjusting the air input of the air stones and the air distributor to be uniformly mixed with the materials. On the premise of ensuring the dissolved oxygen level in the fermentation process, on one hand, the energy consumption is greatly reduced, and on the other hand, the damage of the traditional airlift fermentation tank to the shearing force of the violent aeration for improving the dissolved oxygen to culture microorganisms or animal and plant cells is reduced.

Description

Oxygenation and mixing device of low-shear-force airlift fermentation tank

Technical Field

The utility model belongs to the field of bioengineering, and relates to an aeration device for fermentation liquor of an airlift fermentation tank, in particular to a device for increasing oxygen and promoting uniform mixing of materials in culture of microalgae cells, animal cells and plant cells sensitive to shearing force.

Background

The airlift fermentation tank has the advantages of simple structure, low energy consumption, high oxygen supply efficiency, high fluidization efficiency and the like. The airlift fermentation tank which is widely applied in the biological industry comprises an airlift internal circulation fermentation tank, a gas-liquid double-jet airlift circulation fermentation tank and a tower type airlift fermentation tank provided with a plurality of layers of distribution plates. The air is dispersed in the liquid in the form of bubbles by the air nozzle, and the average density of the liquid decreases on the side where air is introduced and the density of the liquid increases on the side where air is not introduced, thereby causing a difference in density between the liquid in the aeration side and the liquid in the aeration side, and forming a circulation of the liquid in the fermentation tank. However, some cultures such as microalgae cells, animal cells, plant protoplasts and the like are sensitive to shearing force due to the large cells and no cell wall protection, and the high-speed air flow increased by the traditional airlift fermentation tank for increasing the uniform mixing of dissolved oxygen and materials can damage the cultures, thereby limiting the applicability of the traditional airlift fermentation tank in the cell culture.

Through patent search, the air-lift fermentation tank improvement focuses on increasing or improving stirring devices, defoaming devices and other devices to improve the oxygen dissolving efficiency and the process control efficiency (such as CN208151359U, CN208151345U, CN208151345U, CN209923330U, CN105219618B, CN112358952A, CN202576406U, CN211947007U and CN207646189U), or improving baffle plates or a flow guide pipe structure to increase the circulation and mixing efficiency (such as CN211522177U, CN210620795U, CN102796650A, CN111662803A, CN105039150B, CN209010511U, CN208667695U and CN210886007U), improving an air distributor structure (such as CN204569910U, CN203754704U, CN102250752B, CN105602837A, CN210048760U and CN211847927U), enhancing the fermentation parameter process control capability, such as adding a PLC controller or a light, sound and magnetic control system (such as CN 108485951A) or comprehensively improving a fermentation tank structure to increase the mixing efficiency (CN 209412225U). The airlift fermentation tank increases the shearing force of liquid in order to increase the mixing efficiency of dissolved oxygen and fermentation liquor, and is not suitable for the cell fermentation process sensitive to the shearing force.

Compared with the prior art, the utility model has the advantages that the air stone capable of ejecting bubbles with diameters from nanometer to micron is adopted for air supply, the gas-liquid contact surface is greatly increased under the condition of low-intensity ventilation, and compared with the traditional airlift fermentation tank, after the utility model is additionally arranged, the gas-liquid contact surface is greatly increased under the condition of equal-intensity ventilation, the dissolved oxygen efficiency is obviously improved, and high-intensity airflow is prevented from being introduced, so that the generation of overhigh shearing force is avoided; on the other hand, the mixing efficiency of the fermentation liquor can be greatly enhanced by introducing the bottom air stone. The number of the air stones and the number of the connecting pipe wheels for fixing the air stones can be adjusted according to the aerobic degree of the fermentation cells, and a top cover is added above the gas outlet at the bottom to guide the direction of the airflow so that the settled cells are suspended again to enter the gas-liquid circulating flow. The aeration and mixing device of the airlift fermentation tank can obviously increase the dissolved oxygen and mixing efficiency and greatly reduce the energy consumption of the air compressor.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a device which has high dissolved oxygen and mixing efficiency, low shearing force, low modification cost, strong practicability and low energy consumption and is suitable for fermenting and culturing microalgae, animal and plant cells for a traditional airlift fermentation tank.

The technical scheme for solving the technical problem is as follows:

the utility model provides a low shearing force airlift fermentation cylinder oxygenation and mixing arrangement which characterized in that: comprises a gas stone (1), a connecting pipe (2), a gas guide cup (19), a one-way gas valve (10), a gas distributor (6), a bottom gas stone (23), a thread (20) and a heat-resistant sealing ring (21). The ring-arranged air stone (1) and the bottom air stone (23) are oxygen-increasing air bubble stone or air disk stone or an air atomizer of a fish tank or an oxygen-increasing head or an air bubble disk or an air disk or an oxygen pump sand head or nano air disk stone or nano air bubble stone or high-dissolved oxygen cake or oxygen stone or other porous stones formed by sintering. The gas stone (1) is vertically connected to the connecting pipe (2) through a thread (20), and the gas stone head can face upwards or downwards or is parallel to or vertical to the tank bottom (3). The bottom air stone (23) is connected to the air outlet end of the connecting pipe (2) through a thread (20), the air stone head faces the metal cover plate (24), and the connecting pipe is provided with a one-way air valve (6). A heat-resistant sealing ring (21) is arranged in a thread (20) fixedly connecting the annular gas stone (1) or the bottom gas stone (23) with the connecting pipe (2). A metal gas diversion cup (19) is arranged between the annular gas stone (1) and the connecting pipe, and the orientation of the gas stone head and the opening direction of the gas diversion cup are adjusted by adjusting the position of a thread welded on the connecting pipe according to the circulation flow direction of the required gas-liquid mixed liquid along the diversion cylinder. The center of the bottom of the gas diversion cup (19) is provided with a round hole which is sleeved and fixed on the thread.

Preferably, the connecting pipes of the fixed ring aerostone (1) are divided into two groups.

Preferably, each group of connecting pipes is connected with 8 annular aerostones (1).

The connecting pipe is provided with a one-way air valve. The gas distributor (6) comprises a bottom air stone (23), a connecting pipe (2), a thread (20), a heat-resistant sealing ring (21), a metal cover plate (24), a one-way gas valve (10) and a metal rod (22).

Preferably, a circular metal cover plate (24) with the diameter larger than that of the air outlet is fixed 0.5cm above the air outlet at the bottom of the tank, and the circular metal cover plate (24) is welded and fixed on the bottom of the pipe or the air outlet by three metal rods (22). The function of guiding air is achieved.

The utility model has the following beneficial effects:

1. this device can assemble on traditional fermentation cylinder, reforms transform with low costsly, and the practicality is strong, only need install connecting pipe, screw thread, air stone, air cushion, water conservancy diversion piece additional on current air supply system and in bottom air distributor top welding sheetmetal can.

2. The bubbles sprayed by the device are nano-sized to micron-sized bubbles, the gas-liquid contact area is greatly increased, and the dissolved oxygen of the fermentation liquor can be effectively improved under lower air pressure, so that the energy consumption of the air compressor is greatly reduced. Because the small bubbles burst and the shearing force is weak, the damage to microalgae cells, animal cells, plant cells and other shear force sensitive cultures is greatly reduced.

3. This device has reformed transform the air distributor of traditional airlift fermentation cylinder bottom, has avoided under the lower air pressure, and the gas mixing action weakens, and the problem that the cell subsides reaches the purpose that has both improved dissolved oxygen and guaranteed the material and mix.

Drawings

FIG. 1 is a schematic front view of the present invention installed on an airlift fermenter;

FIG. 2 is a schematic structural view of the gas ring in the present invention;

FIG. 3 is a schematic view of the structure of the thread and the heat-resistant seal ring of the present invention;

FIG. 4 is a schematic view of a gas distributor according to the present invention;

FIG. 5 is a schematic side view of the present invention mounted on an airlift fermenter;

FIG. 6 is a schematic view of a lid mounted to an end of an airlift fermenter; (ii) a

In the figure, 1, a gas stone is arranged in a ring mode, 2, a connecting pipe, 3, a tank bottom, 4, a guide cylinder, 5, a middle cooling jacket, 6, a gas distributor, 7, a discharge port, 8, a discharge port valve, 9, a bottom cooling jacket, 10, a one-way gas valve, 11, a material supplementing port, 12, a seed receiving port, 13, a thermometer sleeve, 14, a sensor sleeve, 15, a sensor sleeve, 16, a sight hole, 17, a sampling pipe, 18, a flange, 19, a gas guide cup, 20, threads, 21, a heat-resistant sealing ring, 22, a metal rod, 23, a bottom gas stone and 24, a metal cover plate.

Detailed Description

The utility model will be further described with reference to the following examples, but the scope of the utility model is not limited to the examples. Those skilled in the art can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the scope of the present invention is defined by the claims appended to this specification.

The utility model provides an oxygenation and mixing device for a low-shear-force airlift fermentation tank, which is a device for improving the dissolved oxygen and mixing efficiency of the airlift fermentation tank during low-shear-force cell culture.

Referring to fig. 1-6, the aeration and mixing device for the low-shear airlift fermentation tank is installed on the airlift fermentation tank and comprises an annular air stone (1), a connecting pipe (2), an air guide cup (19), a one-way air valve (10), an air distributor (6), a bottom air stone (23), a thread (20) and a heat-resistant sealing ring (21). The ring aerostone (1) and the bottom aerostone (23) are oxygen-increasing air stones. The annular gas stone (1) is vertically connected to the connecting pipe (2) through a thread (20), and the head of the gas stone faces downwards and is vertical to the bottom of the tank. The bottom air stone (23) is connected to the air outlet end of the connecting pipe (2) through a thread (20), the air stone head faces the metal cover plate (24), and the connecting pipe is provided with a one-way air valve (6). The threads (20) for fixedly connecting the annular gas stones (1), the bottom gas stones (23) and the connecting pipe (2) are internally provided with heat-resistant sealing rings (21). The bottom of the metal gas diversion cup (19) is provided with a round hole which is sleeved on the thread connecting the annular gas stone (1) and the connecting pipe, and the metal gas diversion cup is clamped and fixed after the annular gas stone is tightly connected with the connecting pipe. Two groups of connecting pipes for fixing the annular air stones (1); the number of the annular gas stones (1) connected by each group of connecting pipes is 8. The connecting pipe is provided with a one-way air valve. The gas distributor (6) comprises a bottom air stone (23), a connecting pipe (2), a thread (20), a heat-resistant sealing ring (21), a metal cover plate (24), a one-way gas valve (10) and a metal rod (22). A round metal cover sheet (24) with the diameter larger than that of the air outlet is fixed at the position 0.5cm above the air outlet at the bottom of the tank, and the round metal cover sheet (24) is welded and fixed at the bottom of the pipe or on the air outlet by three metal rods (22). The function of guiding air is achieved.

The airlift fermentation tank comprises a tank body, a guide cylinder (4), a middle cooling jacket (5), a discharge port (7), a discharge port valve (8), a bottom cooling jacket (9), a feeding port (11), a inoculation port (12), a thermometer sleeve (13), a pH sensor sleeve (14), a dissolved oxygen sensor sleeve (15), a sight hole (16), a sampling tube (17) and a flange (18) besides the low-shear-force airlift fermentation tank oxygenation and mixing device.

The device is suitable for microalgae and animal cells without cell walls in the fermentation industry, such as culture sensitive to shearing force, such as euglena gracilis, Vero cells, paclitaxel protoplasts and the like.

The operation of the device is explained as follows:

referring to fig. 1, 2 and 4, sterile compressed air is introduced into the fermentation tank through a connecting pipe, and is diffused through the annular gas stone and the bottom gas stone to form micro bubbles which are mixed with fermentation liquor to form a low-density gas-liquid mixture; after bubbles sprayed from the ring aerostones are sprayed to the bottom of the tank, the bubbles enter a channel between the guide flow cylinder and the wall of the tank to move upwards after backflow, and fermentation liquor at the center of the guide flow cylinder sinks to form circulation to realize the mixing of the fermentation liquor; the micro air bubbles sprayed from the bottom gas stone are mixed with the fermentation liquor and then move upwards, are guided by the metal cover plate and then move along the direction of the tank bottom, are mixed with the gas-liquid flow formed by the annular gas stone, and re-suspend and mix the cells settled at the tank bottom and the discharge hole.

According to the reading of the dissolved oxygen sensor (15), under the condition of fixing the ventilation volume of the connecting pipe (2) of the bottom air stone (23), the number of groups of ventilation of the connecting pipes (2) of the annular air stone (1) and the ventilation volume of each group of connecting pipes are adjusted to control the dissolved oxygen level of the fermentation liquor. Under the condition of low dissolved oxygen, the air supply of the connecting pipe (2) of the annular air stone (1) is stopped, and the ventilation quantity of the connecting pipe (2) of the bottom air stone (23) is adjusted to control the dissolved oxygen level, the mixing of the materials and the cell suspension.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims

1. The utility model provides a low shearing force airlift fermentation cylinder oxygenation and mixing arrangement which characterized in that: the gas distributor comprises a ring array air stone (1), a connecting pipe (2) for supplying gas to the ring array air stone and fixing the ring array air stone, a gas guide cup (19) positioned around the ring array air stone, a one-way gas valve (10) on the connecting pipe (2), a thread (20) for fixing the ring array air stone, a heat-resistant sealing ring (21) in the thread, a bottom air stone (23) positioned on a bottom vent pipe, a round metal cover plate (24) above the bottom air stone, and a gas distributor (6) consisting of the bottom air stone (23) and the round metal cover plate (24).

2. The aeration and mixing device of low shear force airlift fermentation tank of claim 1, wherein said air stones (1) and bottom air stones are aeration bubble stones or air disk stones or fish tank air atomizer or aeration head or bubble disk or air disk or oxygen pump sand head or nano air disk stones or high dissolved oxygen cake or oxygen stone or other porous stones formed by sintering.

3. The aeration and mixing apparatus of low shear airlift fermenter according to claim 1, wherein the ring of air stones (1) is vertically connected to the connecting pipe (2) by a screw thread (20), and the air stones are oriented upward or downward or parallel or vertical to the bottom (3) of the fermenter.

4. The aeration and mixing apparatus of low shear airlift fermenter according to claim 1, wherein the screw thread (20) for fixedly connecting the annular air stone (1) or the bottom air stone (23) and the connecting pipe (2) is provided with a heat-resistant sealing ring (21).

5. The aeration and mixing device of low shear force airlift fermentation tank of claim 1, wherein a metal gas diversion cup (19) is provided between the annular air stone (1) and the connecting pipe (2), and the orientation of the air stone head and the opening direction of the gas diversion cup are adjusted by adjusting the position of the thread welded on the connecting pipe according to the circulation flow direction of the required gas-liquid mixture along the diversion cylinder.

6. The aeration and mixing device of low shear airlift fermenter according to claim 1, wherein the bottom of the gas diversion cup (19) has a circular hole at its center, and is fixed on the screw thread by sleeving.

7. The aeration and mixing apparatus of low shear airlift fermenter according to claim 1, wherein the number of the connecting pipes for fixing the aerators (1) is two, but not limited to two, groups, and the number of the aerators (1) connected to each group of the connecting pipes is 8, but not limited to 8.

8. The aeration and mixing apparatus of low shear airlift fermentor according to claim 1, characterized in that the connecting tube is provided with a one-way air valve.

9. The low shear airlift fermentor oxygenation and mixing device of claim 1, characterized in that: the gas distributor (6) comprises a bottom air stone (23), a connecting pipe (2), a thread (20), a heat-resistant sealing ring (21), a metal cover plate (24), a one-way gas valve (10) and a metal rod (22).

10. The oxygenation and mixing device of low shear airlift fermentation tank of claim 1, wherein a circular metal cover (24) with diameter larger than that of the air outlet is fixed 0.1-3 cm above the air outlet at the bottom of the tank, and the circular metal cover is welded by three metal rods (22) and fixed on the bottom of the pipe or the air outlet to guide air.

11. The aeration and mixing apparatus of low shear airlift fermenter according to claim 1, wherein the bottom air stone (23) is connected to the air outlet end of the connecting pipe (2) by a screw thread (20), and the air stone head faces the metal cover plate (24).