CN216236985U - Device for extracting DNA by adsorption column method - Google Patents

Abstract

The utility model provides a device for extracting DNA by an adsorption column method, which comprises a mounting seat, a seat cavity, a sealing plate, a right plastic column, a left plastic column, a right supporting edge, a left supporting edge, a rotating motor, a PLC, a switch, a power line, a first pulley, a second pulley, a dismounting extraction cylinder structure and a sealing protective cover structure, wherein the seat cavity is arranged in the mounting seat; the sealing plate bolts are arranged at the lower parts of the right plastic column and the left plastic column; the right plastic column is respectively and integrally arranged at the front part and the rear part on the right side of the upper part of the inner wall of the seat cavity; the left plastic column is respectively and integrally arranged at the front part and the rear part on the left side of the upper part of the inner wall of the seat cavity; the right supporting edge and the left supporting edge are respectively glued on the left side and the right side of the lower surface of the sealing plate. The utility model has the beneficial effects that: through the setting of a dismouting extraction section of thick bamboo structure, during the use, can place the work that a large amount of DNA solution carried out extraction at the working chamber thereupon, and then increased the functionality.

Description

Device for extracting DNA by adsorption column method

Technical Field

The utility model belongs to the technical field of DNA extraction processing, and particularly relates to a device for extracting DNA by an adsorption column method.

Background

The medical apparatus and instruments are the most basic elements of medical, scientific research, teaching, institutions and clinical discipline work; including both professional and home medical equipment. It refers to instruments, devices, appliances, materials or other articles that use the human body alone or in combination, and also includes the required software. The centrifuge is widely applied to the fields of clinical medicine, biochemistry, immunology, blood stations and the like, is a conventional instrument for centrifugal sedimentation in a laboratory, can rotate, and enables a rotated object to do sedimentation motion under the action of centrifugal force, so that the separation of the object is realized by utilizing the difference of sedimentation speeds of different substances in a centrifugal vertical field. Traditional medical centrifuge test capacity is little, and quantity through the fixed centrifuging tube of support is limited, and operation process is loaded down with trivial details, and is inefficient, and the noise is great in the use, and prior art is that chinese patent publication is a technical scheme that DNA extraction element of CN201720698137.8 adopted is: the centrifugal machine comprises a machine shell, wherein a centrifugal cavity is arranged in the machine shell, a centrifugal mechanism is arranged in the centrifugal cavity, the centrifugal mechanism comprises a vibration motor, a transmission shaft and an off-line tube plate, the output end of the vibration motor is connected with one end of the transmission shaft, the center of the centrifugal tube plate is provided with a shaft hole, and the other end of the transmission shaft is connected with the centrifugal tube plate through the shaft hole and is fixed through a sealing ring; a plurality of centrifugal tube holes for fixing the centrifugal tubes are uniformly distributed on the centrifugal tube plate, the bottoms of the centrifugal tube holes are conical, and the depth of the centrifugal tube holes is consistent with the length of the centrifugal tubes; the inner wall of the centrifugal cavity is provided with a suction material; the inner wall of the shell is provided with a plurality of heaters.

However, the existing device for extracting DNA by the adsorption column method has the problems that a large amount of DNA solution is inconvenient to place for processing, the replacement and treatment are inconvenient, and the sealing effect is poor.

Therefore, it is necessary to invent a device for extracting DNA by adsorption column method.

SUMMERY OF THE UTILITY MODEL

In order to solve the technical problems, the utility model provides a device for extracting DNA by an adsorption column method, which aims to solve the problems that the existing cutting device for machining fan equipment is poor in protection effect, inconvenient to observe the working condition, poor in protection effect, inconvenient to radiate and inconvenient to fix parts with different sizes. A device for extracting DNA by an adsorption column method comprises a mounting seat, a seat cavity, a sealing plate, a right plastic column, a left plastic column, a right supporting edge, a left supporting edge, a rotating motor, a PLC (programmable logic controller), a switch, a power line, a first pulley, a second pulley, a dismounting extraction cylinder structure and a sealing protective cover structure, wherein the seat cavity is arranged in the mounting seat; the sealing plate bolts are arranged at the lower parts of the right plastic column and the left plastic column; the right plastic column is respectively and integrally arranged at the front part and the rear part on the right side of the upper part of the inner wall of the seat cavity; the left plastic column is respectively and integrally arranged at the front part and the rear part on the left side of the upper part of the inner wall of the seat cavity; the right supporting edge and the left supporting edge are respectively glued on the left side and the right side of the lower surface of the sealing plate; the rotating motor is mounted on the upper part of the inner wall of the seat cavity through a bolt; the PLC bolt is arranged on the right side of the front surface of the mounting seat; the switch is electrically connected to the front surface of the PLC; the power line is electrically connected to the right side of the PLC; the first pulley and the second pulley are respectively installed on the left side and the right side of the upper surface of the installation seat through bolts; the dismounting and extracting cylinder structure is arranged at the upper part of the mounting seat; the sealing protective cover structure is arranged at the upper part of the dismounting and mounting extraction cylinder structure; the detachable extraction cylinder structure comprises a working cylinder, a working cavity, a sleeve joint, an insertion column, a reinforcing strip, a connecting sleeve joint, a fastening bolt and a detachable adsorption working frame structure, wherein the working cavity is formed in the working cylinder; the sleeve joint is integrally arranged in the middle of the lower end of the working barrel; the plug-in column is integrally arranged at the upper end of the connecting sleeve head; the reinforcing strip is glued on the front surface of the splicing column; the fastening bolt is in threaded connection with the front surface of the connecting sleeve head; the detachable adsorption working frame structure is arranged in the working cavity.

Preferably, the detachable adsorption working frame structure comprises a detachable ring, a first connecting hole, an assembling ring, a clamping cover, a second connecting hole, a sealing ring and a filter screen, wherein the detachable ring is integrally arranged in the middle of the inner wall of the working cavity; the first connecting hole is formed in the middle of the inner part of the dismounting ring; the assembling ring is integrally arranged at the upper end of the disassembling and assembling ring; the second through hole is formed above the inner part of the clamping cover; the sealing ring and the filter screen are respectively arranged above the inner part of the clamping cover.

Preferably, the sealing protective cover structure comprises a sealing cover, a cover cavity, a first connecting column, a second connecting column, a grasping cross rod and an encryption sleeve, wherein the cover cavity is formed in the lower side of the inner part of the sealing cover; the first connecting column and the second connecting column are respectively glued on the middle left side and the middle right side of the front surface of the sealing cover; the grabbing cross rod is glued at the front ends of the first connecting column and the second connecting column; the encryption sleeve is embedded on the inner wall of the cover cavity.

Preferably, the working barrel is a PVC plastic barrel, and a working cavity is formed in the working barrel.

Preferably, the upper parts of the splicing column and the reinforcing bar are inserted into the sleeve joint, and the front part of the sleeve joint is in threaded connection with the wing bolt.

Preferably, the connecting sleeve is sleeved on an output shaft of the rotating motor and is screwed tightly to fix the connecting sleeve.

Preferably, the filter screen adopts the nitrocellulose filter screen, the filter screen setting in the inside middle part of working chamber.

Preferably, the clamping cover is in threaded connection with the upper part of the assembling ring, and the second through hole in the clamping cover is communicated with the filter screen.

Preferably, the sealing ring is a rubber ring and is arranged at the upper part of the filter screen.

Preferably, the cover cavity inside the sealing cover is in threaded connection with the upper part of the working barrel.

Preferably, first spliced pole, second spliced pole and grabbing horizontal pole equipment be the U type, grabbing horizontal pole outer wall on be provided with word antiskid line.

Compared with the prior art, the utility model has the beneficial effects that:

1. in the utility model, the working barrel is a PVC plastic barrel, and the working cavity is arranged in the working barrel, so that a large amount of DNA solution can be placed in the working cavity for extraction when the device is used, and the functionality is further improved.

2. In the utility model, the upper parts of the splicing column and the reinforcing bar are inserted into the sleeve joint, and the front part of the sleeve joint is in threaded connection with the wing bolt, so that the sleeve joint is convenient to mount or dismount and is convenient to replace or perform other work.

3. In the utility model, the connecting sleeve is sleeved on the output shaft of the rotating motor and is screwed up and fixed by the fastening bolt, so that the detachable extraction cylinder structure is more reasonably installed and used.

4. In the utility model, the filter screen is a nitrocellulose filter screen, the filter screen is arranged in the middle part of the inner part of the working cavity, RNA and protein in DNA solution can be adsorbed into the filter screen in the working process, and double-stranded DNA enters the lower part of the working cavity.

5. In the utility model, the clamping cover is in threaded connection with the upper part of the assembling ring, and the second through hole in the clamping cover is communicated with the filter screen, so that the clamping cover is more reasonably matched with the filter screen for dismounting and mounting, and meanwhile, the solution can conveniently pass through the filter screen for use.

6. In the utility model, the sealing ring is a rubber ring and is arranged on the upper part of the filter screen, so that the sealing effect is improved during installation, and the problem of leakage easily occurs on the surface.

7. In the utility model, the cover cavity in the sealing cover is in threaded connection with the upper part of the working barrel, so that the sealing property is improved when the sealing cover is used, and the problem of spilling occurs in the working process.

8. According to the utility model, the first connecting column, the second connecting column and the grabbing cross rod are assembled into a U shape, and the outer wall of the grabbing cross rod is provided with the straight anti-skidding lines, so that the grabbing cross rod can be conveniently grabbed and held to drive the sealing cover to rotate for dismounting.

Drawings

Fig. 1 is a schematic structural view of the present invention.

Fig. 2 is a schematic structural view of the structure of the removable extraction cartridge of the present invention.

Fig. 3 is a schematic structural view of the detachable suction frame structure of the present invention.

Fig. 4 is a schematic structural diagram of the sealing protection cover structure of the present invention.

Fig. 5 is a schematic diagram of the electrical wiring of the present invention.

In the figure:

1. a mounting seat; 2. a seat cavity; 3. a sealing plate; 4. a right plastic post; 5. a left plastic post; 6. a right support edge; 7. a left support edge; 8. a rotating electric machine; 9. a PLC; 10. a switch; 11. a power line; 12. a first pulley; 13. a second pulley; 14. disassembling and assembling the extraction cylinder structure; 141. a working barrel; 142. a working chamber; 143. sleeving a joint; 144. inserting the column; 145. reinforcing the strips; 146. connecting a sleeve head; 147. fastening a bolt; 148. a detachable adsorption working frame structure; 1481. disassembling and assembling the ring; 1482. a first communication hole; 1483. assembling a ring; 1484. a cover is clamped; 1485. a second through hole; 1486. a seal ring; 1487. filtering with a screen; 15. sealing the protective cover structure; 151. a sealing cover; 152. a cover cavity; 153. a first connecting column; 154. a second connecting column; 155. grasping a cross bar; 156. and (4) encrypting the sleeve.

Detailed Description

The utility model is further described below with reference to the accompanying drawings:

example (b):

as shown in fig. 1 and fig. 2, an apparatus for extracting DNA by adsorption column method comprises a mounting base 1, a base cavity 2, a sealing plate 3, a right plastic column 4, a left plastic column 5, a right supporting edge 6, a left supporting edge 7, a rotating motor 8, a PLC9, a switch 10, a power line 11, a first pulley 12, a second pulley 13, a detachable extraction cylinder structure 14 and a sealing protection cover structure 15, wherein the base cavity 2 is arranged inside the mounting base 1; the sealing plate 3 is mounted at the lower parts of the right plastic column 4 and the left plastic column 5 through bolts; the right plastic column 4 is respectively and integrally arranged at the front part and the rear part on the right side of the upper part of the inner wall of the seat cavity 2; the left plastic column 5 is respectively and integrally arranged at the front part and the rear part on the left side of the upper part of the inner wall of the seat cavity 2; the right supporting edge 6 and the left supporting edge 7 are respectively glued on the left side and the right side of the lower surface of the sealing plate 3; the rotating motor 8 is arranged on the upper part of the inner wall of the seat cavity 2 through a bolt; the PLC9 bolt is arranged on the right side of the front surface of the mounting seat 1; the switch 10 is electrically connected to the front surface of the PLC 9; the power line 11 is electrically connected to the right side of the PLC 9; the first pulley 12 and the second pulley 13 are respectively installed on the left side and the right side of the upper surface of the installation seat 1 through bolts; the dismounting and extracting cylinder structure 14 is arranged at the upper part of the mounting seat 1; the sealing protective cover structure 15 is arranged at the upper part of the dismounting and mounting extraction cylinder structure 14; the detachable extraction cylinder structure 14 comprises a working cylinder 141, a working cavity 142, a sleeve joint 143, an insertion column 144, a reinforcing bar 145, a connecting sleeve joint 146, a fastening bolt 147 and a detachable adsorption working frame structure 148, wherein the working cylinder 141 is specifically made of a PVC plastic cylinder, and the working cavity 142 is formed in the working cylinder, so that a large amount of DNA solution can be placed in the working cavity 142 for extraction when the detachable extraction cylinder structure is used, and the functionality is further improved; the working cavity 142 is arranged inside the working barrel 141; the sleeve joint 143 is integrally arranged in the middle of the lower end of the working barrel 141; the plug-in column 144 is integrally arranged at the upper end of the connecting sleeve head 146; the upper parts of the splicing column 144 and the reinforcing bar 145 are inserted into the sleeve joint 143, and the front part of the sleeve joint 143 is in threaded connection with a wing bolt, so that the installation or disassembly is convenient, and the replacement or other work is convenient; the reinforcing strip 145 is glued on the front surface of the inserting column 144; the connecting sleeve head 146 is sleeved on the output shaft of the rotating motor 8 and is screwed up and fixed by the fastening bolt 147, so that the dismounting and mounting extraction cylinder structure 14 is more reasonably mounted and used; the fastening bolt 147 is screwed on the front surface of the connecting sleeve head 146; the removable suction frame structure 148 is mounted within the working chamber 142.

As shown in fig. 3, in the above embodiment, specifically, the detachable suction working frame structure 148 includes a detachable ring 1481, a first through hole 1482, an assembling ring 1483, a clamping cover 1484, a second through hole 1485, a sealing ring 1486, and a filter screen 1487, where the detachable ring 1481 is integrally disposed in a middle portion of an inner wall of the working chamber 142; the first connecting hole 1482 is formed in the middle of the inside of the dismounting ring 1481; the assembling ring 1483 is integrally arranged at the upper end of the disassembling ring 1481; the clamping cover 1484 is in threaded connection with the upper part of the assembling ring 1483, and the second through holes 1485 in the clamping cover 1484 are communicated with the filter screen 1487, so that the filter screen 1487 can be more reasonably matched for dismounting, and meanwhile, the solution can conveniently pass through the filter screen 1487 for use; the second through hole 1485 is formed above the inside of the clamping cover 1484; the sealing ring 1486 and the filter screen 1487 are respectively placed above the inside of the clamping cover 1484; the sealing ring 1486 is a rubber ring, and the sealing ring 1486 is arranged on the upper part of the filter screen 1487, so that when the filter screen is installed, the sealing effect is improved, and the problem of leakage is easily caused on the surface; the filter screen 1487 is a nitrocellulose filter screen, the filter screen 1487 is arranged in the middle of the inside of the working chamber 142, during the working process, the RNA and protein in the DNA solution can be adsorbed into the filter screen 1487, and the double-stranded DNA enters the lower part of the inside of the working chamber 142.

As shown in fig. 4, in the above embodiment, specifically, the sealing protection cover structure 15 includes a sealing cover 151, a cover cavity 152, a first connecting column 153, a second connecting column 154, a gripping cross bar 155 and an encryption sleeve 156, the cover cavity 152 inside the sealing cover 151 is screwed on the upper portion of the working barrel 141, when in use, the sealing performance is increased, and further, in the working process, a spilling problem occurs; the cover cavity 152 is arranged at the lower side of the inner part of the sealing cover 151; the first connecting column 153 and the second connecting column 154 are respectively glued to the middle left side and the middle right side of the front surface of the sealing cover 151; the first connecting column 153, the second connecting column 154 and the grabbing cross rod 155 are assembled into a U shape, and the outer wall of the grabbing cross rod 155 is provided with straight anti-skid lines, so that the grabbing cross rod 155 can be grabbed and held conveniently to drive the sealing cover 151 to rotate for disassembly and assembly; the grabbing cross bar 155 is glued to the front ends of the first connecting column 153 and the second connecting column 154; the encryption sleeve 156 is mounted on the inner wall of the cap cavity 152.

In the above embodiment, the rotating electrical machine 8 is a speed reducing motor with model number 5IK120RGU-CF/5GU 30K.

In the above embodiment, specifically, the PLC9 is a PLC of model FX 2N-48.

In the above embodiment, specifically, the switch 10 is electrically connected to an input end of the PLC9, and the rotating electrical machine 8 is electrically connected to an output end of the PLC 9.

Principle of operation

The working principle of the utility model is as follows: before use, the filter screen 1487 is placed on the upper portion of the assembly ring 1483, the clamping cover 1484 is rotated to the assembly ring 1483, the filter screen 1487 can be fixed accordingly, after initial installation is completed, a DNA solution to be extracted is poured into the working cavity 142 of the working cylinder 141, the working cylinder 141 is moved, the sleeve joint 143 is sleeved on the plug column 144 and the reinforcing bar 145, the wing bolt in the front portion of the sleeve joint 143 is screwed to be fixed, after fixing is completed, the grasping cross bar 155 is grasped, the cover cavity 152 of the sealing cover 151 is rotated to the working cylinder 141, sealing can be achieved, when the rotary motor 8 is started to rotate the working cylinder 141, RNA and protein in the DNA solution can be adsorbed into the filter screen 1487, and double-stranded DNA enters the lower portion of the working cavity 142, and extraction can be completed.

The technical solutions of the present invention or similar technical solutions designed by those skilled in the art based on the teachings of the technical solutions of the present invention are all within the scope of the present invention.

Claims (10)

1. The device for extracting the DNA by the adsorption column method is characterized by comprising a mounting seat (1), a seat cavity (2), a sealing plate (3), a right plastic column (4), a left plastic column (5), a right supporting edge (6), a left supporting edge (7), a rotating motor (8), a PLC (programmable logic controller) (9), a switch (10), a power line (11), a first pulley (12), a second pulley (13), a dismounting extraction cylinder structure (14) and a sealing protective cover structure (15), wherein the seat cavity (2) is arranged in the mounting seat (1); the sealing plate (3) is mounted at the lower parts of the right plastic column (4) and the left plastic column (5) through bolts; the right plastic column (4) is respectively and integrally arranged at the front part and the rear part on the right side of the upper part of the inner wall of the seat cavity (2); the left plastic column (5) is respectively and integrally arranged at the front part and the rear part of the left side of the upper part of the inner wall of the seat cavity (2); the right supporting edge (6) and the left supporting edge (7) are respectively glued to the left side and the right side of the lower surface of the sealing plate (3); the rotating motor (8) is arranged on the upper part of the inner wall of the seat cavity (2) through a bolt; the PLC (9) is installed on the right side of the front surface of the installation seat (1) through a bolt; the switch (10) is electrically connected to the front surface of the PLC (9); the power line (11) is electrically connected to the right side of the PLC (9); the first pulley (12) and the second pulley (13) are respectively installed on the left side and the right side of the upper surface of the installation seat (1) through bolts; the dismounting and extracting cylinder structure (14) is arranged at the upper part of the mounting seat (1); the sealing protective cover structure (15) is arranged at the upper part of the dismounting extraction cylinder structure (14); the detachable extraction cylinder structure (14) comprises a working cylinder (141), a working cavity (142), a sleeve joint (143), an insertion column (144), a reinforcing strip (145), a connecting sleeve joint (146), a fastening bolt (147) and a detachable adsorption working frame structure (148), wherein the working cavity (142) is arranged in the working cylinder (141); the sleeve joint (143) is integrally arranged in the middle of the lower end of the working barrel (141); the inserting column (144) is integrally arranged at the upper end of the connecting sleeve head (146); the reinforcing strip (145) is glued on the front surface of the splicing column (144); the fastening bolt (147) is in threaded connection with the front surface of the connecting sleeve head (146); the detachable adsorption working frame structure (148) is arranged in the working cavity (142).

2. The device for extracting DNA by the adsorption column method according to claim 1, wherein the detachable adsorption working frame structure (148) comprises a detachable ring (1481), a first through hole (1482), an assembly ring (1483), a clamping cover (1484), a second through hole (1485), a sealing ring (1486) and a filter screen (1487), and the detachable ring (1481) is integrally arranged at the middle part of the inner wall of the working chamber (142); the first connecting hole (1482) is formed in the middle part inside the dismounting ring (1481); the assembling ring (1483) is integrally arranged at the upper end of the disassembling ring (1481); the second through hole (1485) is formed above the inner part of the clamping cover (1484); the sealing ring (1486) and the filter screen (1487) are respectively arranged above the inner part of the clamping cover (1484).

3. The device for extracting DNA by adsorption column method as claimed in claim 1, wherein said sealing protective cover structure (15) comprises a sealing cover (151), a cover cavity (152), a first connecting column (153), a second connecting column (154), a grasping cross bar (155) and an encryption sleeve (156), said cover cavity (152) is opened at the lower side of the inside of the sealing cover (151); the first connecting column (153) and the second connecting column (154) are respectively glued on the middle left side and the middle right side of the front surface of the sealing cover (151); the grabbing cross bar (155) is glued at the front ends of the first connecting column (153) and the second connecting column (154); the encryption sleeve (156) is embedded on the inner wall of the cover cavity (152).

4. The device for extracting DNA by the adsorption column method according to claim 1, wherein the working cylinder (141) is a PVC plastic cylinder and is internally provided with a working cavity (142).

5. The device for extracting DNA by the adsorption column method according to claim 1, wherein the upper parts of the insertion column (144) and the reinforcing bar (145) are inserted into the socket (143), and the front part of the socket (143) is connected with a wing bolt by screw thread.

6. The apparatus for extracting DNA by adsorption column method according to claim 1, wherein said connecting socket (146) is sleeved on the output shaft of the rotating motor (8) and is fastened and fixed by tightening the fastening bolt (147).

7. The apparatus for extracting DNA by adsorption column method according to claim 2, wherein said filter (1487) is a nitrocellulose filter, and said filter (1487) is disposed at a middle portion inside the working chamber (142).

8. The apparatus for extracting DNA by adsorption column method according to claim 2, wherein said cap (1484) is screwed to the upper part of the assembling ring (1483), and the second through hole (1485) in the cap (1484) is communicated with the strainer (1487).

9. The apparatus for extracting DNA by adsorption column method according to claim 3, wherein the cap chamber (152) inside the cap (151) is screw-coupled to the upper portion of the cylinder (141).

10. The device for extracting DNA by adsorption column method according to claim 3, wherein the first connecting column (153), the second connecting column (154) and the grasping cross bar (155) are assembled into a U shape, and the outer wall of the grasping cross bar (155) is provided with a straight anti-slip line.

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