CN216207833U - Solid phase micro-extraction chip and solid phase micro-extraction sampling separation enrichment device - Google Patents
Solid phase micro-extraction chip and solid phase micro-extraction sampling separation enrichment device Download PDFInfo
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- CN216207833U CN216207833U CN202122358635.7U CN202122358635U CN216207833U CN 216207833 U CN216207833 U CN 216207833U CN 202122358635 U CN202122358635 U CN 202122358635U CN 216207833 U CN216207833 U CN 216207833U
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Abstract
The utility model relates to the technical field of pharmaceutical analytical instruments, and discloses a solid-phase microextraction chip and a solid-phase microextraction sampling, separating and enriching device. Wherein, solid phase micro-extraction chip includes: a chip body; a containing cavity for containing solid phase micro-extraction filler is arranged in the chip body; a first opening and a second opening are formed in the side wall of the chip body; the first opening and the second opening are respectively communicated with the accommodating cavity; and a grid for blocking the solid-phase micro-extraction filler from flowing out is arranged at the second opening. The method can retain the nonpolar compound through strong hydrophobic effect, other components permeate the adsorbent to flow out of the chip, and the target object is selectively eluted by using the eluent, so that the separation, purification and enrichment of the complex sample are realized.
Description
Technical Field
The utility model relates to the technical field of pharmaceutical analysis instruments, in particular to a solid-phase micro-extraction chip and a solid-phase micro-extraction sampling, separating and enriching device.
Background
The drug enters the systemic circulation to exert the drug effect and needs to be dissolved and absorbed in the body. After the drug is absorbed into blood, two states exist, one is plasma protein binding type and the other is free type. The tissue distribution of the drug in the body is mainly completed by the free drug in the plasma. Therefore, the study of the concentration of the free drug is of great significance for evaluating the efficacy and toxicity of the drug.
The existing treatment methods for measuring the concentration of free drugs in blood plasma mainly comprise a balance dialysis method, an ultrafiltration centrifugation method, an ultracentrifugation method, a gel filtration method and the like, but all of the methods have certain defects and cannot meet the requirement of monitoring the concentration of a drug living body in real time.
SUMMERY OF THE UTILITY MODEL
The purpose of the utility model is: provides a solid phase micro-extraction chip which can realize the separation, purification and enrichment of complex samples on line.
In order to achieve the above object, the present invention provides a solid phase micro-extraction chip, comprising: a chip body; a containing cavity for containing solid phase micro-extraction filler is arranged in the chip body; a first opening and a second opening are formed in the side wall of the chip body; the first opening and the second opening are respectively communicated with the accommodating cavity; and a grid for blocking the solid-phase micro-extraction filler from flowing out is arranged at the second opening.
Optionally, a polyethylene glycol coating is disposed on the surface of the chip body and the sidewall of the accommodating cavity.
Optionally, the first opening and the second opening are respectively circular; the diameter of the first opening and the second opening is 1 mm.
Optionally, the length of the chip body is 1cm-5cm, and the width of the chip body is 1mm-10 mm.
Optionally, the glass plate is further included; the chip body is arranged on the glass plate.
A solid phase micro extraction sampling separation enrichment device comprises: the injection pump is used for perfusing the experimental animal, the probe is used for extracting the target substance in the experimental animal body, and the solid phase micro-extraction chip is used for extracting the target substance from the experimental animal body; the output end of the injection pump is communicated with the first opening; one end of the probe is communicated with the injection pump, and the other end of the probe is communicated with the first opening.
Optionally, the method further includes: an injector for injecting an eluent; the output end of the injector is communicated with the first opening.
Optionally, the method further includes: a mass spectrometer for detecting the concentration and metabolic profile of the target substance; the input end of the mass spectrometer is communicated with the second opening.
Optionally, the mass spectrometer is a combined LC-MS.
Compared with the prior art, the solid phase micro-extraction chip and the solid phase micro-extraction sampling, separating and enriching device provided by the embodiment of the utility model have the beneficial effects that:
according to the embodiment of the utility model, the nonpolar compound can be retained through the strong hydrophobic effect, other components flow out of the chip through the adsorbent, and the target object is selectively eluted by using the eluent, so that the separation, purification and enrichment of the complex sample are realized.
Drawings
FIG. 1 is a schematic structural diagram of an embodiment of the present invention;
fig. 2 is a schematic diagram of a partial structure of a chip body according to an embodiment of the present invention.
In the figure, 1, a chip body; 11. a first opening; 12. a second opening; 2. an injection pump; 3. A probe; 4. an injector; 5. a mass spectrometer; 6. a glass plate.
Detailed Description
The following detailed description of embodiments of the present invention is provided in connection with the accompanying drawings and examples. The following examples are intended to illustrate the utility model but are not intended to limit the scope of the utility model.
First, it should be noted that the orientations of top, bottom, upward, downward, and the like referred to herein are defined with respect to the orientation in the respective drawings, are relative concepts, and thus can be changed according to different positions and different practical states in which they are located. These and other orientations, therefore, should not be used in a limiting sense.
It should be noted that the term "comprising" does not exclude other elements or steps and the "a" or "an" does not exclude a plurality.
Furthermore, it should be further noted that any single technical feature described or implied in the embodiments herein, or any single technical feature shown or implied in the figures, can still be combined between these technical features (or their equivalents) to obtain other embodiments of the present application not directly mentioned herein.
It will be further understood that the terms "first," "second," and the like, are used herein to describe various information and should not be limited to these terms, which are used merely to distinguish one type of information from another. For example, "first" information may also be referred to as "second" information, and similarly, "second" information may also be referred to as "first" information, without departing from the scope of the present application.
It should be noted that in different drawings, the same reference numerals indicate the same or substantially the same components.
As shown in fig. 1, a solid phase micro-extraction chip according to a preferred embodiment of the present invention includes: a chip body 1; and a containing cavity for containing the solid-phase micro-extraction filler is formed in the chip body 1. A first opening 11 and a second opening 12 are formed in the side wall of the chip body 1, and the first opening 11 and the second opening 12 are respectively communicated with the accommodating cavity. A grid for blocking the outflow of the solid phase micro extraction packing is arranged at the second opening 12.
The solid-phase micro-extraction chip plays a role in desalting, enriching and eluting target substances. The first opening 11 on the chip body 1 is an input port, the second opening 12 is an output port, a target substance enters the accommodating cavity from the first opening 11 and is in contact with a solid phase microextraction filler in the accommodating cavity, the solid phase microextraction filler is a common reversed phase adsorbent, a nonpolar compound is retained through a strong hydrophobic effect, other components penetrate through the adsorbent and flow out of the chip, the target substance is selectively eluted by using an eluent, and separation, purification and enrichment of a complex sample are realized. In addition, a grid is arranged on the second opening 12, and the grid can block the solid-phase micro-extraction filler from flowing out.
As a preferred embodiment of the chip type living solid phase micro-extraction device system, the chip material is selected from polydimethylsiloxane or polymethyl methacrylate.
More preferably, the solid phase microextraction packing comprises C18, C8 molecular separation and purification solid phase microextraction packing
Specifically, the solid-phase microextraction filler is injected into the accommodating cavity in a mode that a part of the solid-phase microextraction filler is placed in methanol, a long gun head is fixed by a 1ml syringe to absorb the suspended solid-phase microextraction filler-methanol mixed solution, the mixed solution is injected into the accommodating cavity from the first opening 11, and the mixed solution is filled for a plurality of times in a small amount until the accommodating cavity is filled with the solid-phase microextraction filler.
Further, the surface of the chip body 1 and the side wall of the accommodating cavity are provided with a polyethylene glycol coating (PEG). Wherein, the chip body 1 is soaked in 0.5 percent PEG20000, ultrasonic treatment is carried out for 30min, and the chip body is placed for 2h, so that the surface of the chip body 1 and the side wall of the containing cavity are decorated with a layer of polyethylene glycol coating (PEG).
Further, the first opening 11 and the second opening 12 are respectively circular; the first opening 11 and the second opening 12 are circular in cross section, and the first opening 11 and the second opening 12 have a diameter of 1 mm.
Further, the length of the chip body 1 is 1cm-5cm, and the width is 1mm-10 mm.
Further, the present application also includes a glass plate 6; the chip body 1 is arranged on the glass plate 6.
The application still provides a solid phase micro-extraction sampling separation enrichment facility, and it includes: a syringe pump 2 for perfusing the experimental animal, a probe 3 for extracting the target substance in the experimental animal, and a solid phase micro-extraction chip as described above. The output end of the injection pump 2 is communicated with the first opening 11, one end of the probe 3 is communicated with the injection pump 2, and the other end of the probe 3 is communicated with the first opening 11.
The syringe pump 2 continuously perfuses normal saline into the body of the experimental animal at a certain flow rate, and the probe 3 is disposed in the tissue organ of the experimental animal, and the normal saline injected by the syringe pump 2 carries out the target substance extracted from the tissue organ by the probe 3 and flows into the accommodating cavity from the first opening 11. And the target substance and the solid phase microextraction filler in the accommodating cavity are common reversed phase adsorbents, nonpolar compounds are retained through strong hydrophobic effect, other components permeate the adsorbents and flow out of the chip, and the target substance is selectively eluted by using eluent, so that separation, purification and enrichment of complex samples are realized.
Specifically, the probe used was blood probe CMA 20.
Further, this application still includes: a syringe 4 for injecting an eluent, an output end of the syringe 4 being in communication with the first opening 11.
Specifically, the eluent is 90% methanol-10% 0.1% formic acid water as eluent, and the elution is carried out at a flow rate of 3uL/min for 30 min.
Further, this application still includes: a mass spectrometer 5 for detecting the concentration and metabolic profile of the target substance. The input end of the mass spectrometer 5 communicates with the second opening 12.
Further, the mass spectrometer 5 is a combined LC-MS.
The working process of the utility model is as follows: the syringe pump 2 continuously perfuses normal saline into the body of the experimental animal at a certain flow rate, and the probe 3 is disposed in the tissue organ of the experimental animal, and the normal saline injected by the syringe pump 2 carries out the target substance extracted from the tissue organ by the probe 3 and flows into the accommodating cavity from the first opening 11. And the target substance and the solid phase microextraction filler in the accommodating cavity are common reversed phase adsorbents, nonpolar compounds are retained through strong hydrophobic effect, other components permeate the adsorbents and flow out of the chip, and the target substance is selectively eluted by using eluent, so that separation, purification and enrichment of complex samples are realized. The concentration and metabolism of the target substance are then detected by a combination of LC-MS.
In summary, the embodiments of the present invention provide a solid phase microextraction sampling, separating and enriching device, which can implement real-time detection on a target substance, thereby implementing real-time free concentration analysis on a living body of a drug.
The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and substitutions can be made without departing from the technical principle of the present invention, and these modifications and substitutions should also be regarded as the protection scope of the present invention.
Claims (9)
1. A solid phase microextraction chip, comprising: a chip body; a containing cavity for containing solid phase micro-extraction filler is arranged in the chip body; a first opening and a second opening are formed in the side wall of the chip body; the first opening and the second opening are respectively communicated with the accommodating cavity; and a grid for blocking the solid-phase micro-extraction filler from flowing out is arranged at the second opening.
2. The solid phase microextraction chip according to claim 1, wherein polyethylene glycol coating is provided on both the surface of said chip body and the side wall of said containing cavity.
3. The solid phase microextraction chip according to claim 1, wherein said first opening and said second opening are each circular; the diameter of the first opening and the second opening is 1 mm.
4. The solid phase microextraction chip according to claim 1, wherein said chip body has a length of 1cm-5cm and a width of 1mm-10 mm.
5. The solid phase microextraction chip according to claim 1, further comprising a glass plate; the chip body is arranged on the glass plate.
6. The utility model provides a solid phase micro-extraction sampling separation enrichment facility which characterized in that includes: a syringe pump for perfusing a laboratory animal, a probe for extracting a target substance in a laboratory animal, and the solid phase microextraction chip according to any one of claims 1-5; the output end of the injection pump is communicated with the first opening; one end of the probe is communicated with the injection pump, and the other end of the probe is communicated with the first opening.
7. The solid phase microextraction sampling, separating and enriching device according to claim 6, further comprising: an injector for injecting an eluent; the output end of the injector is communicated with the first opening.
8. The solid phase microextraction sampling, separating and enriching device according to claim 6, further comprising: a mass spectrometer for detecting the concentration and metabolic profile of the target substance; the input end of the mass spectrometer is communicated with the second opening.
9. The solid phase microextraction, sampling, separating and enriching device according to claim 8, wherein the mass spectrometer is a combined LC-MS.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122358635.7U CN216207833U (en) | 2021-09-27 | 2021-09-27 | Solid phase micro-extraction chip and solid phase micro-extraction sampling separation enrichment device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122358635.7U CN216207833U (en) | 2021-09-27 | 2021-09-27 | Solid phase micro-extraction chip and solid phase micro-extraction sampling separation enrichment device |
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| Publication Number | Publication Date |
|---|---|
| CN216207833U true CN216207833U (en) | 2022-04-05 |
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| CN202122358635.7U Active CN216207833U (en) | 2021-09-27 | 2021-09-27 | Solid phase micro-extraction chip and solid phase micro-extraction sampling separation enrichment device |
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| CN (1) | CN216207833U (en) |
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- 2021-09-27 CN CN202122358635.7U patent/CN216207833U/en active Active
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