CN216192187U

CN216192187U - Stem cell separation and extraction device

Info

Publication number: CN216192187U
Application number: CN202122202377.3U
Authority: CN
Inventors: 刘燕婷
Original assignee: Shanghai Wanfu Medical Technology Co ltd
Current assignee: Shanghai Wanfu Medical Technology Co ltd
Priority date: 2021-09-13
Filing date: 2021-09-13
Publication date: 2022-04-05
Anticipated expiration: 2031-09-13

Abstract

The utility model discloses a stem cell separating and extracting device, which relates to the technical field of bioengineering and comprises a working box, wherein a box cover is arranged at the top of the working box, an isolating plate is arranged on the inner wall of the working box, a motor is arranged on the inner wall of the working box and positioned below the isolating plate, the output end of the motor is fixedly connected with a transmission rod, one end of the transmission rod, which is far away from the motor, is fixedly connected with a rotary rod, the top of the rotary rod is fixedly connected with a centrifugal cylinder, the inner wall of the centrifugal cylinder is provided with a fixing plate, a test tube cylinder is arranged in the fixing plate, and one end of a small spring is provided with a baffle plate. According to the utility model, the fixing plate and the test tube barrel are arranged, and the test tube barrel is arranged in the fixing plate in an inclined manner, so that the test tube is in an inclined state in centrifugal operation, and the centrifugal force applied to the test tube closer to the tail part of the test tube is larger, thereby effectively solving the problem of incomplete stem cell component layering after centrifugal operation.

Description

Stem cell separation and extraction device

Technical Field

The utility model relates to the technical field of bioengineering, in particular to a stem cell separation and extraction device.

Background

Stem cells are a class of cells with unlimited or immortal self-renewal capacity, capable of producing at least one type of highly differentiated progeny cells, the definition of stem cells has been continuously revised over the years and defined at different levels, and most biologists and physicians now consider stem cells to be a class of cells from embryonic, fetal or adult bodies that have unlimited self-renewal and proliferative differentiation capacity under certain conditions, capable of producing daughter cells with the same phenotype as genotype and self, capable of producing specialized cells that make up body tissues and organs, and capable of differentiating into progenitor cells.

At present, stem cell generally adopts centrifuge to go on when carrying out the separation and draw, and the test tube that is equipped with stem cell carries out centrifugal operation when putting into centrifuge, and high-speed rotation probably leads to the in vitro liquid to rock and with test tube stopper jack-up, leads to the failure to the stem cell separation extraction, and the centrifuge during operation that uses at present, the test tube is in the vertical state, is unfavorable for carrying out the layering to the different components of separation extraction back cell.

Disclosure of Invention

The utility model aims to: in order to solve the problems that liquid in a pipe is splashed due to high-speed rotation during centrifugal operation and cell components are not layered completely after centrifugation is finished, a stem cell separation and extraction device is provided.

In order to achieve the purpose, the utility model provides the following technical scheme: the utility model provides a stem cell separation extraction element, includes the work box, the top of work box is provided with the case lid, the inner wall of work box is provided with the division board, the below that work box inner wall is located the division board is provided with the motor, the output fixedly connected with transfer line of motor, the one end fixedly connected with rotary rod of motor is kept away from to the transfer line, the top fixedly connected with centrifuge tube of rotary rod, centrifuge tube's top is provided with sealed lid, the bottom of sealed lid is provided with the rubber block, centrifuge tube's inner wall is provided with the fixed plate, the inside of fixed plate is provided with the test tube section of thick bamboo, be provided with the little spring in the test tube section of thick bamboo, the one end of little spring is provided with the baffle.

Preferably, the case lid is connected with the work box through the pivot is rotated, and the bottom of case lid and the closed position of work box contact are provided with the rubber packing ring.

Preferably, one end of the transmission rod penetrates through the isolation plate and is fixedly connected with the bottom of the rotating rod.

Preferably, the top of rubber block fixed mounting has the fixed block, the fixed block passes through the bearing and is connected with the bottom of sealed lid is rotated.

Preferably, the outer wall of the sealing cover is provided with external threads, and the outer wall of the top end of the centrifugal cylinder is provided with internal threads matched with the inner wall of the sealing cover.

Preferably, the number of the test tube barrels is eight in a group, and the test tube barrels are uniformly distributed in the centrifugal barrel.

Compared with the prior art, the utility model has the beneficial effects that:

1. according to the utility model, the bearing, the fixed block baffle and the spring are arranged, the rubber block is arranged on one side of the fixed block, which is far away from the bearing, and the small spring and the baffle are arranged in the test tube barrel;

2. according to the utility model, the fixing plate and the test tube barrel are arranged, and the test tube barrel is arranged in the fixing plate in an inclined manner, so that the test tube is in an inclined state in centrifugal operation, and stem cell components closer to the tail part of the test tube are subjected to larger centrifugal force in centrifugal operation, thereby effectively solving the problem of incomplete stem cell component layering after centrifugal operation.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a top view of the present invention;

FIG. 3 is a top view of a centrifuge bowl of the present invention;

fig. 4 is a schematic structural view of the sealing cover of the present invention.

In the figure: 1. a work box; 2. a box cover; 3. a separator plate; 4. a motor; 5. a transmission rod; 6. rotating the rod; 7. a centrifugal cylinder; 8. a sealing cover; 9. a fixing plate; 10. testing the tube barrel; 11. a small spring; 12. a baffle plate; 13. a rubber block; 14. connecting blocks; 15. and a bearing.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "disposed" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art. The following describes an embodiment of the present invention based on its overall structure.

The motors (90 YYJJ) mentioned in the present invention are available in the market or in private order.

Referring to fig. 1-4, a stem cell separating and extracting device comprises a working box 1, a box cover 2 is arranged at the top of the working box 1, an isolation plate 3 is arranged on the inner wall of the working box 1, a motor 4 is arranged on the inner wall of the working box 1 and located below the isolation plate 3, a transmission rod 5 is fixedly connected to the output end of the motor 4, a rotary rod 6 is fixedly connected to one end, away from the motor 4, of the transmission rod 5, a centrifugal cylinder 7 is fixedly connected to the top of the rotary rod 6, a sealing cover 8 is arranged at the top of the centrifugal cylinder 7, a rubber block 13 is arranged at the bottom of the sealing cover 8, a fixing plate 9 is arranged on the inner wall of the centrifugal cylinder 7, a test tube cylinder 10 is arranged inside the fixing plate 9, a small spring 11 is arranged inside the test tube cylinder 10, and a baffle 12 is arranged at one end of the small spring 11.

Please refer to fig. 1, the box cover 2 is rotatably connected to the working box 1 through a rotating shaft, and a rubber gasket is disposed at a position where the bottom of the box cover 2 contacts and closes with the working box 1, so as to protect the contact position of the working box 1 and the box cover 2 when the box cover 2 is closed, and facilitate the opening and closing of the box cover 2.

Referring to fig. 1 and 2, one end of the driving rod 5 penetrates through the isolation plate 3 and is fixedly connected to the bottom of the rotating rod 6, so as to fix the centrifugal cylinder 7 to the rotating rod 6 more stably, which is beneficial to the stable rotation of the centrifugal cylinder 7 during the centrifugal operation.

Please refer to fig. 4, a fixed block 14 is fixedly installed at the top of the rubber block 13, the fixed block 14 is rotatably connected with the bottom of the sealing cover 8 through a bearing 15, the rubber block 13 can not rotate through the bearing 15 when the sealing cover 8 rotates, and the falling of the test tube stopper caused by the friction of the rubber block 13 on the test tube stopper when the rubber block 13 rotates is avoided.

Please refer to fig. 1, the outer wall of the sealing cover 8 is provided with an external thread, and the outer wall of the top end of the centrifuge tube 7 is provided with an internal thread matched with the inner wall of the sealing cover 8, so that the sealing cover 8 can be attached to the centrifuge tube 7 more effectively, and the sealing effect is better.

Please refer to fig. 3, the number of the test tubes 10 is eight, and the test tubes are uniformly distributed inside the centrifuge tube 7, which is beneficial to improve the working efficiency of the device, and the test tubes are uniformly stressed.

The working principle is as follows: when preparing to carry out centrifugal operation on stem cells in a test tube, firstly opening a box cover 2 above a working box 1, then unscrewing a sealing cover 8 at the top of a centrifugal cylinder 7 clockwise, then putting test tubes needing centrifugal operation into the test tube cylinder 10 in sequence, then screwing the sealing cover 8 anticlockwise, rotating the sealing cover 8 to drive a fixed block 14 to move downwards under the action of a bearing 15, thereby realizing the fixation of the test tubes placed in the test tube cylinder 10, extruding the test tubes to move downwards on the inner wall of the test tube cylinder 10 while a rubber block 13 moves downwards, further extruding the small springs 11 by the test tubes through a baffle plate 12, fixing the test tubes in the test tube cylinder 10 by the deformation of the small springs 11 and the deformation of the rubber block 13, ensuring that the test tube plugs cannot be ejected when rotating at high speed, then closing the box cover 2, then opening a motor 4, and driving a transmission rod 5 to rotate by the motor 4, the transfer line 5 drives the rotary rod 6 rotatory, rotary rod 6 is together rotatory rather than the fixed centrifugal tube 7 in top, centrifugal tube 7 is rotatory to drive its inside test tube and is rotated, thereby carry out centrifugal operation to the stem cell in the test tube, after waiting for centrifugation, open case lid 2, rotate sealed lid 8 again and open, the test tube that drives baffle 12 top under little spring 11's effect outwards removes, thereby be convenient for take out the test tube in the test tube section of thick bamboo 10, accomplish the centrifugal operation to the stem cell promptly.

It will be evident to those skilled in the art that the utility model is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the utility model being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims

1. The utility model provides a stem cell separation and extraction device, includes work box (1), its characterized in that: the top of the working box (1) is provided with a box cover (2), the inner wall of the working box (1) is provided with a separation plate (3), a motor (4) is arranged on the inner wall of the working box (1) below the isolation plate (3), the output end of the motor (4) is fixedly connected with a transmission rod (5), one end of the transmission rod (5) far away from the motor (4) is fixedly connected with a rotating rod (6), the top of the rotating rod (6) is fixedly connected with a centrifugal cylinder (7), the top of the centrifugal cylinder (7) is provided with a sealing cover (8), the bottom of the sealing cover (8) is provided with a rubber block (13), the inner wall of the centrifugal cylinder (7) is provided with a fixing plate (9), the test tube holder is characterized in that a test tube holder (10) is arranged in the fixing plate (9), a small spring (11) is arranged in the test tube holder (10), and a baffle (12) is arranged at one end of the small spring (11).

2. The stem cell separating and extracting device according to claim 1, wherein: the box cover (2) is rotatably connected with the working box (1) through a rotating shaft, and a rubber gasket is arranged at the position where the bottom of the box cover (2) is closed to contact with the working box (1).

3. The stem cell separating and extracting device according to claim 1, wherein: one end of the transmission rod (5) penetrates through the isolation plate (3) and is fixedly connected with the bottom of the rotating rod (6).

4. The stem cell separating and extracting device according to claim 1, wherein: the top fixed mounting of rubber block (13) has fixed block (14), fixed block (14) are connected with the bottom of sealed lid (8) through bearing (15) are rotated.

5. The stem cell separating and extracting device according to claim 1, wherein: the outer wall of the sealing cover (8) is provided with external threads, and the outer wall of the top end of the centrifugal cylinder (7) is provided with internal threads matched with the inner wall of the sealing cover (8).

6. The stem cell separating and extracting device according to claim 1, wherein: the number of the test tube barrels (10) is eight in a group, and the test tube barrels are uniformly distributed in the centrifugal tube (7).