CN216164779U - Cell procedure refrigerating device - Google Patents
Cell procedure refrigerating device Download PDFInfo
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- CN216164779U CN216164779U CN202121161375.8U CN202121161375U CN216164779U CN 216164779 U CN216164779 U CN 216164779U CN 202121161375 U CN202121161375 U CN 202121161375U CN 216164779 U CN216164779 U CN 216164779U
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- 238000000034 method Methods 0.000 title claims description 30
- 238000004321 preservation Methods 0.000 claims abstract description 51
- 230000008014 freezing Effects 0.000 claims abstract description 36
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 32
- 238000007710 freezing Methods 0.000 claims abstract description 28
- 239000007788 liquid Substances 0.000 claims abstract description 20
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 16
- 230000007246 mechanism Effects 0.000 claims description 14
- 239000000463 material Substances 0.000 claims description 8
- 239000006261 foam material Substances 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 2
- 239000002994 raw material Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 6
- 238000011084 recovery Methods 0.000 abstract description 4
- 238000009529 body temperature measurement Methods 0.000 abstract description 3
- 210000004027 cell Anatomy 0.000 description 47
- 239000000523 sample Substances 0.000 description 8
- 238000001816 cooling Methods 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 238000001514 detection method Methods 0.000 description 4
- 238000005315 distribution function Methods 0.000 description 4
- 238000005138 cryopreservation Methods 0.000 description 3
- 238000003958 fumigation Methods 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000009413 insulation Methods 0.000 description 2
- 238000005057 refrigeration Methods 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000003028 elevating effect Effects 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000003223 protective agent Substances 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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Abstract
The utility model discloses a cell program freezing device, which comprises a base, a heat preservation cavity, a pipe frame, a temperature measuring element, a lifting device and a control device, wherein the base is provided with a heat preservation cavity; the heat preservation cavity is arranged on the base and is used for containing liquid nitrogen; the cell preservation tube is arranged on the tube frame; the temperature measuring elements are at least provided with two temperature measuring elements for measuring the temperature of the same height position at the inner side and the outer side of the cell preservation tube at the same time respectively; the lifting device is arranged on the base, and the output end of the lifting device is fixedly connected with the pipe support to drive the pipe support to lift in the heat preservation cavity; the control device is electrically connected with the temperature measuring element to acquire temperature measuring data, and the control device is electrically connected with the lifting device to control the pipe frame to lift in the heat preservation cavity. The utility model has reasonable structural design, and can effectively improve the cell freezing effect and the recovery rate through real-time temperature measurement and lifting control.
Description
Technical Field
The utility model relates to the technical field of cell freezing, in particular to a cell program freezing device.
Background
In recent years, with the overall advance of modern biomedicine to molecular medicine and the age of individual diagnosis and treatment, the demand for large-scale and high-quality biological specimens and related information resources is also sharply increased. Many developed countries attach great importance to the protection and development of human genetic resources, establish various biological sample banks and population-based biological banks and virtual biological banks, and regard them as strategic measures for developing the core competitiveness of the biomedical field. Establishing a biological cell resource library, storing biological cells (most of sperm cells, embryos or egg cells and stem cells at present), and having important significance for the fields of species resource storage, breeding, propagation and the like.
Program cooling is widely applied to the cryopreservation process of cells as an effective cooling means, wherein a liquid nitrogen fumigation method is widely adopted due to simple equipment. According to the method, liquid nitrogen is pre-cooled by utilizing different gas layer temperatures on the surface of the liquid nitrogen, and after pre-cooling is completed, a sample is immersed in the liquid nitrogen to complete freezing.
However, since the surface temperature of liquid nitrogen is not fixed, during the freezing process, when the cell freezing operation is performed in a manual control manner, the temperature reduction rate required by the cell sample is often inconsistent with the pre-cooling temperature, so that the cell preservation effect is very different.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a cell program freezing device, aiming at solving the problem that the temperature reduction rate and the precooling temperature are difficult to ensure to be consistent manually in the cell program freezing process in the prior art.
In order to achieve the purpose, the technical scheme of the utility model is as follows:
a cell program freezing device comprises a freezing chamber,
a base;
the heat preservation cavity is arranged on the base, and liquid nitrogen is contained in the heat preservation cavity;
the cell preservation pipe is arranged on the pipe frame;
at least two temperature measuring elements are arranged to measure the temperature of the same height position on the inner side and the outer side of the cell preservation tube at the same time respectively;
the lifting device is arranged on the base, and the output end of the lifting device is fixedly connected with the pipe frame so as to drive the pipe frame to lift in the heat preservation cavity;
and the control device is electrically connected with the temperature measuring element to acquire temperature measuring data, and the control device is electrically connected with the lifting device to control the pipe frame to lift in the heat preservation cavity.
Preferably, the temperature measuring element is a thermocouple.
Preferably, the lifting device comprises a linear driving mechanism and a bent arm, the linear driving mechanism is vertically installed on the base, one end of the bent arm is fixed on the output end of the linear driving mechanism, and the other end of the bent arm is fixedly connected with the pipe frame; wherein, the linear driving mechanism comprises an electric push rod, an air cylinder or a hydraulic cylinder.
Further, still include the display screen, display screen fixed mounting be in on the base, just the display screen with controlling means electric connection.
Preferably, the display screen is a touch display screen.
Preferably, the heat preservation cavity is a vacuum cup or a cylindrical structure made of a material with low heat conductivity coefficient.
Preferably, the low thermal conductivity material comprises a foam material or an EVA material.
Further, the device also comprises a control tube, wherein the control tube is fixedly arranged on the tube frame; wherein the temperature measuring element is disposed within the control tube to indirectly obtain the temperature inside the cell preservation tube.
Preferably, the control tube is arranged at the same height as the cell preservation tube.
Adopt above-mentioned technical scheme, because temperature element, elevating gear and controlling means's setting, make and preserve the in-process that the pipe descends in the heat preservation intracavity that contains liquid nitrogen at control cell, it is intraductal through constantly acquireing the cell preservation, the temperature of outer both sides, thereby can ensure to descend in-process cell preservation intraductally, the difference in temperature of outer both sides is less than predetermined difference in temperature threshold value all the time, and can stop descending when the difference in temperature exceeds this difference in temperature threshold value, continue to descend when the difference in temperature is less than this difference in temperature threshold value, until reaching predetermined heat preservation temperature position, for manual operation, can effectively improve the save effect of procedure freezing in-process cell.
Drawings
FIG. 1 is a schematic diagram of a freezing apparatus for cell procedure according to the present invention;
FIG. 2 is a diagram illustrating a temperature distribution function of an insulating chamber according to an embodiment.
In the figure, 1-a control device, 2-a display screen, 3-a heat preservation cavity, 4-a pipe frame, 5-a contrast pipe, 6-a cell preservation pipe, 7-a first temperature measuring element, 8-a second temperature measuring element, 9-a bent arm, 10-a sliding block, 11-a screw rod, 12-a guide rail, 13-a motor and 14-a base.
Detailed Description
The following further describes embodiments of the present invention with reference to the drawings. It should be noted that the description of the embodiments is provided to help understanding of the present invention, but the present invention is not limited thereto. In addition, the technical features involved in the embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
It should be noted that in the description of the present invention, the terms "upper", "lower", "left", "right", "front", "rear", and the like indicate orientations or positional relationships based on structures shown in the drawings, and are only used for convenience in describing the present invention, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention.
In the technical scheme, the terms "first" and "second" are only used for referring to the same or similar structures or corresponding structures with similar functions, and are not used for ranking the importance of the structures, or comparing the sizes or other meanings.
In addition, unless expressly stated or limited otherwise, the terms "mounted" and "connected" are to be construed broadly, e.g., the connection may be a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; the two structures can be directly connected or indirectly connected through an intermediate medium, and the two structures can be communicated with each other. To those skilled in the art, the specific meanings of the above terms in the present invention can be understood in light of the present general concepts, in connection with the specific context of the scheme.
Example one
A cell program freezing device is shown in figure 1 and comprises a control device 1, a heat preservation cavity 3, a pipe frame 4, a cell preservation pipe 6, a temperature measuring element, a lifting device and a base 14.
Wherein the base 14 is configured as a platform that is mounted on a table or floor in use. The heat preservation chamber 3 is configured to be a cylindrical structure made of a material with low heat conductivity coefficient (such as a foam material or an EVA material), and an opening is formed at the top of the heat preservation chamber; or the heat preservation cavity 3 is configured as a vacuum cup. In use, the insulating chamber 3 contains liquid nitrogen, and the liquid level of the liquid nitrogen is usually a certain distance, for example 10cm, from the upper edge of the insulating chamber 3. The temperature distribution of the heat-retaining chamber 3 above the liquid surface satisfies a temperature distribution function T ═ a × h2+ bxh + c, where h is the distance from the temperature measuring point to the upper edge of the thermal insulation cavity, T is the temperature at the temperature measuring point, a, b, and c are constants and obtained through experiments, as shown in fig. 2, which is the temperature distribution function of the thermal insulation cavity 3 selected in this embodimentSchematic of numbers.
The cell preservation tube 6 is used for loading cells to be frozen, and the cell preservation tube 6 is fixedly installed on the tube support 4, so that the tube support 4 drives the cell preservation tube 6 to ascend and descend in the heat preservation cavity 3, and further programmed cooling is performed.
And the lifting device is arranged on the base 14, and the output end of the lifting device is fixedly connected with the pipe frame 4 so as to drive the pipe frame 4 to lift in the heat preservation cavity 3. In this embodiment, the lifting device includes a linear driving mechanism and a curved arm 9, wherein the linear driving mechanism is vertically installed on the base 14, one end of the curved arm 9 is fixed on the output end of the linear driving mechanism, the other end of the curved arm is fixedly connected with the pipe frame 4, and the other end of the curved arm 9 has a vertical section with a certain length so as to avoid the vertical section from rubbing against the heat preservation cavity 3 during lifting, for example, the curved arm 9 is configured in a frame structure with a downward opening in this embodiment. Wherein, the linear driving mechanism comprises an electric push rod, an air cylinder or a hydraulic cylinder.
In the present embodiment, the linear driving mechanism is configured as an electric push rod, and as shown in fig. 1, the electric push rod specifically includes a slide block 10, a screw rod 11, a guide rail 12 and a motor 13. Wherein, the guide rail 12 is vertically arranged and fixed on the base 14; the screw 11 is parallel to the guide rail 12, and the upper end and the lower end of the screw 11 are respectively rotatably connected to a transverse plate at the top of the guide rail 12 and the base 14 through bearings; the motor 13 is fixed on the base 14, and an output shaft of the motor 13 is connected with the lower end of the screw rod 11 through a coupler or a gear transmission mechanism; slider 10 sliding connection is on guide rail 12, and is provided with the screw hole with screw rod 11 looks adaptation on the slider 10 to make motor 13 start the back, drive screw rod 11 and rotate, drive slider 10 by screw rod 11 again and go up and down, and drive pipe support 4 and the cell preservation pipe 6 of installation on it and can go up and down in heat preservation chamber 3 through bent arm 9.
In addition, the temperature of the inside and outside of the cell preservation tube 6 can be measured by at least two temperature measuring elements at the same time, for example, in this embodiment, the temperature measuring elements are configured to include a first temperature measuring element 7 and a second temperature measuring element 8, and both the first temperature measuring element 7 and the second temperature measuring element 8 are selected to be thermocouples, wherein the probe of the first temperature measuring element 7 extends into the inside of the cell preservation tube 6 to obtain the temperature of the inside thereof, the probe of the second temperature measuring element 8 is located outside the cell preservation tube 6 to obtain the temperature of the outside thereof, and the probe of the first temperature measuring element 7 and the probe of the second temperature measuring element 8 are located at the same height position. It will be appreciated that a plurality of temperature measuring elements (more than one first temperature measuring element 7 and one second temperature measuring element 8) may be provided to increase the accuracy of temperature measurement, thereby reducing the error of single-point measurement on one side of the cell storage tube 6.
In this embodiment, the control device 1 includes an electronic device, the electronic device includes a memory storing executable program codes and a processor coupled to the memory, and the processor is further electrically connected to the temperature measuring element and the lifting device, and is usually further connected to an external device or an upper computer, so that after receiving a relevant instruction sent by the external device or the upper computer, the processor calls the executable program codes stored in the memory, thereby executing the step of the preset cell program freezing method according to the executable program codes.
Specifically, in use, the temperature distribution function T of the holding chamber 3 is first obtained (obtained by experiments in advance) according to the structure thereof2+ bxh + c, and is input to the memory in the control device 1 through an external device or an upper computer for storage; then, the cells to be frozen and the necessary cell protective agent are put into the cell preservation tube 6, and the real-time descending speed v ═ k-b)/2ah of the cell protection tube 6 is obtained according to the required descending speed k (input by external equipment or an upper computer), but of course, other speeds are also possible; then, the processor in the control device 1 controls the lifting device to drive the pipe frame 4 and the cell protection pipe 6 fixedly arranged on the pipe frame to be installed at the speed of descending, wherein h can be obtained by calculating the rotating angle of the motor 13; comparing the temperature difference between the first temperature measuring element 7 and the second temperature measuring element 8 in the descending process to ensure that the difference is lower than a preset temperature difference threshold value, and stopping descending the cell protection tube 6 through the control device 1 when the difference reaches the temperature difference threshold value, so that the temperature data measured by the first temperature measuring element 7 reaches a preset freezing temperatureAnd (4) stopping.
For example, when the device provided by the embodiment is applied to sperm freezing: 1. taking 2ml of healthy sperms of three volunteers respectively, and naturally liquefying in a constant temperature box at 37 ℃; 2. primarily detecting the sperm activity rate by using a sperm counting plate under a microscope, repeating the detection twice for each sample, counting 200 sperms for each detection, comparing the detection values twice until the error percentage of the measurement values of the two times is within an acceptable range, otherwise, preparing the detection sample again and detecting again; 3. the sperm grading method is a grade: fast forward, b stage: slow advance, stage c: in-situ swing, d-stage: the method comprises the following steps of (1) calculating the sperm freezing and recovering rate of the forward movement (sperm freezing and recovering rate for short) without movement: the method comprises the following steps of (percent of frozen a-grade sperms + percent of frozen b-grade sperms)/(percent of pre-frozen a-grade sperms + percent of pre-frozen b-grade sperms), 4, mixing the frozen sperms with a commercially available sperm protection solution in a ratio of 1: 1, respectively putting the mixture into a cell protection tube 6 for freezing, 5, installing a first temperature measuring element 7 and a second temperature measuring element 8, freezing according to the steps, wherein the specific procedure is that the temperature reduction rate is 10K/min, the temperature is reduced to-80 ℃, the pre-freezing is carried out for 20min, the pre-freezing is completed, and then the temperature is continuously reduced to-196 ℃ by 10K/min and the frozen sperm is frozen in liquid nitrogen for 2 weeks and then taken out.
In addition, a control group was set and frozen by an artificial liquid nitrogen fumigation method, i.e., a cryopreservation tube containing sperm and a protective solution was placed 10min at a distance of 10cm from the surface of liquid nitrogen, and then the cryopreservation tube was immersed in liquid nitrogen and stored for 2 weeks and then taken out.
And (4) unfreezing the frozen sperms in warm water at 37 ℃, and after unfreezing is finished, calculating the sperm freezing recovery rate again. As shown in Table 1, it can be seen that the recovery rate of the cells frozen by the method of this example was higher than that of the cells frozen by the artificial liquid nitrogen fumigation method.
TABLE 1-recovery rates for different sperm freezing regimes (significant difference for different superscripts, P <0.05)
It can be seen that the device that this embodiment provided can make cell degree freezing process obtain real-time control and control to can make quick adjustment according to the monitoring result, for the operation of artifical manual mode, can make the refrigeration effect obtain effectual promotion.
Example two
The difference from the first embodiment is that: in this embodiment, the control device 1 further includes a display screen 2, the display screen 2 is electrically connected to the processor in the control device 1, and preferably, the display screen 2 is a touch screen, so that the control parameters (cooling rate k and temperature difference threshold) are input and modified through the display screen 2.
EXAMPLE III
The difference from the first embodiment is that: in this embodiment, as shown in fig. 1, the device further comprises a control tube 5, the control tube 5 is fixedly mounted on the tube frame 4, and the control tube 5 and the cell preservation tube 6 are arranged at the same height, and when the device is used, the control tube 5 is filled with an equal volume of a control solution having the same thermophysical properties (including crystallization enthalpy, thermal conductivity coefficient and specific heat capacity) as those of the liquid in the cell protection tube 6, or directly filled with an equal volume of liquid as the cell protection tube 6. In addition, a temperature measuring element (i.e., a first temperature measuring element 7) is disposed inside the control tube 5 so that the temperature measured by this first temperature measuring element 7 is taken as the temperature inside the cell preservation tube 6 in an indirect measurement manner, and at the same time, the first temperature measuring element 7 disposed inside the cell protection tube 6 in the first embodiment is eliminated.
So set up, can avoid the temperature measurement to treat the influence of freezing cell to further improve cell refrigeration effect.
The embodiments of the present invention have been described in detail with reference to the accompanying drawings, but the present invention is not limited to the described embodiments. It will be apparent to those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the utility model, and the scope of protection is still within the scope of the utility model.
Claims (9)
1. A cell procedure freezing device, characterized in that: comprises the steps of (a) preparing a mixture of a plurality of raw materials,
a base;
the heat preservation cavity is arranged on the base, and liquid nitrogen is contained in the heat preservation cavity;
the cell preservation pipe is arranged on the pipe frame;
at least two temperature measuring elements are arranged to measure the temperature of the same height position on the inner side and the outer side of the cell preservation tube at the same time respectively;
the lifting device is arranged on the base, and the output end of the lifting device is fixedly connected with the pipe frame so as to drive the pipe frame to lift in the heat preservation cavity;
and the control device is electrically connected with the temperature measuring element to acquire temperature measuring data, and the control device is electrically connected with the lifting device to control the pipe frame to lift in the heat preservation cavity.
2. The cell procedure freezing device of claim 1, wherein: the temperature measuring element is a thermocouple.
3. The cell procedure freezing device of claim 1, wherein: the lifting device comprises a linear driving mechanism and a bent arm, the linear driving mechanism is vertically installed on the base, one end of the bent arm is fixed on the output end of the linear driving mechanism, and the other end of the bent arm is fixedly connected with the pipe frame; wherein, the linear driving mechanism comprises an electric push rod, an air cylinder or a hydraulic cylinder.
4. The cell procedure freezing device of claim 1, wherein: still include the display screen, display screen fixed mounting be in on the base, just the display screen with controlling means electric connection.
5. The cell procedure freezing device of claim 4, wherein: the display screen is a touch display screen.
6. The cell procedure freezing device of claim 1, wherein: the heat preservation cavity is a vacuum cup or a cylindrical structure made of a material with low heat conductivity coefficient.
7. The cell procedure freezing device of claim 6, wherein: the low thermal conductivity material comprises a foam material or an EVA material.
8. Cell program freezing apparatus according to any of claims 1-7, wherein: the device also comprises a control tube, wherein the control tube is fixedly arranged on the tube frame; wherein the temperature measuring element is disposed within the control tube to indirectly obtain the temperature inside the cell preservation tube.
9. The cell procedure freezing device of claim 8, wherein: the control tube and the cell preservation tube are arranged at the same height.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121161375.8U CN216164779U (en) | 2021-05-27 | 2021-05-27 | Cell procedure refrigerating device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121161375.8U CN216164779U (en) | 2021-05-27 | 2021-05-27 | Cell procedure refrigerating device |
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| Publication Number | Publication Date |
|---|---|
| CN216164779U true CN216164779U (en) | 2022-04-05 |
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| CN202121161375.8U Active CN216164779U (en) | 2021-05-27 | 2021-05-27 | Cell procedure refrigerating device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115088709A (en) * | 2022-08-07 | 2022-09-23 | 上海安库生医生物科技有限公司 | Freezing tube capable of monitoring internal temperature |
| WO2022247845A1 (en) * | 2021-05-27 | 2022-12-01 | 上海理工大学 | Cell program freezing method, system and apparatus |
-
2021
- 2021-05-27 CN CN202121161375.8U patent/CN216164779U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022247845A1 (en) * | 2021-05-27 | 2022-12-01 | 上海理工大学 | Cell program freezing method, system and apparatus |
| CN115088709A (en) * | 2022-08-07 | 2022-09-23 | 上海安库生医生物科技有限公司 | Freezing tube capable of monitoring internal temperature |
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| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Shanghai Bozhen Medical Technology Co.,Ltd. Assignor: University of Shanghai for Science and Technology Contract record no.: X2023310000150 Denomination of utility model: A Cell Program Freezing Device Granted publication date: 20220405 License type: Common License Record date: 20231016 |