CN216106892U

CN216106892U - Cell mar device

Info

Publication number: CN216106892U
Application number: CN202122739017.7U
Authority: CN
Inventors: 丁鸽; 王妙; 刘东鸣; 刘博�; 何晴; 周巍; 李伟宏; 陈博宇; 毛隆; 黄建春; 李蕊; 赵苗苗; 刘璐璐; 唐振宏; 颜三忠; 石科磊
Original assignee: Shaanxi Medical Device Quality Inspection Institute
Current assignee: Shaanxi Medical Device Quality Inspection Institute
Priority date: 2021-11-09
Filing date: 2021-11-09
Publication date: 2022-03-22
Anticipated expiration: 2031-11-09

Abstract

The utility model belongs to the field of cell culture, and particularly relates to a cell scratching device. Including apron and mar piece, be equipped with the guide way on the apron, mar piece includes support and casing, the mar post is connected to the one end of support, the handle is connected to the other end of support, the inside cavity of casing, the mar post with the support is located the casing is inside and can be followed the inside round trip movement of casing, the outer wall of casing is equipped with the limiting plate, the casing can pass the guide way passes through the limiting plate hang in on the guide way. The utility model has simple structure, simple manufacture and convenient operation, the scribed scratch line is very straight and uniform in width, no damage is caused to the plate surface, the scratch line can be standardized, no pollution is caused to cells, and the problem that the cell scratch experiment is easy to pollute is solved.

Description

Cell mar device

Technical Field

The utility model belongs to the field of cell culture, and particularly relates to a cell scratching device.

Background

The cell scarification (round Healing) method is a method for simply measuring cell migration movement and repair capacity, similar to an in vitro Wound Healing model, and comprises the steps of scribing a line in the central region of cell growth by using a microspur head or other hard objects on monolayer adherent cells cultured in an in vitro culture dish or a flat plate, removing cells in the central part, continuously culturing the cells for an experimental set time (for example, 24 hours), taking out a cell culture plate, and observing whether peripheral cells migrate (repair) to the central scarification region, so as to judge the growth migration capacity of the cells.

In the traditional cell scratching experiment, a ruler is fixed above a pore plate, and then a 1mL sterile gun head is used as an operation tool to manufacture a cell scratching line. When one hand pastes the ruler above the pore plate and the other hand operates above the pore plate by the gun head, the probability of the risk of cell pollution is invisibly increased; and the manual marking is difficult to control the force for holding the gun head, so that the problems of inconsistent width of the marking line, non-straight bending, overlarge force, even damage to the board surface and the like are caused, and the experimental result is influenced.

SUMMERY OF THE UTILITY MODEL

In view of the disadvantages of the conventional scribing method, the utility model aims to provide a cell scribing device which has the advantages of simple structure, easy manufacture, convenient operation, very straight and uniform scribed lines, no damage to the plate surface, capability of standardizing the scribed lines, no pollution to cells and capability of solving the problem of easy pollution of cell scribing experiments.

The utility model provides a cell scratching device which comprises a cover plate and a scratching piece, wherein a guide groove is formed in the cover plate, the scratching piece comprises a support and a shell, one end of the support is connected with a scratching column, the other end of the support is connected with a handle, the shell is hollow, the scratching column and the support are located in the shell and can move back and forth along the interior of the shell, a limiting plate is arranged on the outer wall of the shell, and the shell can pass through the guide groove and be suspended on the guide groove.

The mar post is close to the one end of support is the rectangular bodily form, the mar post is close to the one end of casing is the arc.

The shell is cuboid, and the width of the shell is the same as the groove width of the guide groove.

The support periphery is equipped with the elastic component, the elastic component be located inside the casing and with casing inner wall contacts.

The casing is close to the one end outside of mar post is equipped with the helmet.

When the cover plate is used, the cover plate is covered on the six-hole plate, and the cover plate and the six-hole plate are identical in shape and size.

The guide groove is located at the position where the cover plate corresponds to the hole positions on the six-hole plate, and the length of the guide groove is the same as the diameter of the hole positions.

The cover plate covers the six-hole plate, and the distance from the bottom of the shell to the bottom surface of the six-hole plate is larger than the height of the culture solution in the six-hole plate.

The height from the bottom of the shell to the culture solution in the six-hole plate is 2-3 mm.

The apron with the mar post material is polytetrafluoroethylene.

The utility model has the following beneficial effects:

(1) the utility model provides a cell scratching device, which comprises a cover plate and a scratching part, wherein the cover plate and the scratching part are matched and used together, the structure is simple, the manufacture is simple and easy, and the operation is convenient; after the scribing is completed, the handle is quickly lifted upwards, the handle drives the support and the scribing column to be lifted upwards, no pollution is caused to cells, and the problem that the cell scribing experiment is easy to pollute is solved.

(2) The scribing line scribed by the utility model is very straight and uniform in width, does not damage the plate surface of the six-hole plate, can standardize the scribing line, has the advantages of simple operation, stable result, strong practicability, low cost, repeated use and the like, and has good application prospect.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.

FIG. 1 is a schematic structural diagram of a scoring member according to an embodiment of the present invention;

FIG. 2 is a schematic structural diagram of a cover plate according to an embodiment of the present invention;

description of reference numerals:

1-protective cap, 2-scratch column, 3-elastic piece, 4-handle, 5-limit plate, 6-bracket, 7-shell, 8-cover plate and 9-guide groove.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like, indicate orientations and positional relationships based on those shown in the drawings, and are used only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be considered as limiting the present invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, features defined as "first", "second", may explicitly or implicitly include one or more of the described features. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise. Furthermore, the terms "mounted," "connected," and "connected" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Examples

A cell scratching device comprises a cover plate 8 and a scratching part, wherein the cover plate 8 and the scratching part are matched for use, the cover plate 8 is consistent with a six-hole plate in shape and size, a guide groove 9 is formed in the cover plate 8, the guide groove 9 is located at the position, corresponding to hole positions on the cover plate 8 and the six-hole plate, the guide groove 9 is located at the position of the diameter of the hole positions, and the length of the guide groove 9 is the same as the diameter of the hole positions. The circles in FIG. 2 are schematic hole sites on a six-well plate, and do not actually exist.

Mar piece includes support 6 and casing 7, mar post 2 is connected to the one end of support 6, the one end that mar post 2 is close to support 6 is long cubic, the one end that mar post 2 is close to casing 7 is the arc, handle 4 is connected to the other end of support 6, the inside cavity of casing 7, mar post 2 and support 6 are located casing 7 inside and can follow the inside round trip movement of casing 7, handle 4 is located casing 7's outside, casing 7's outer wall is equipped with limiting plate 5, limiting plate 5 sets up two at casing 7 outer wall bilateral symmetry, casing 7 is long cubic, casing 7's width is the same with the groove width of guide way 9, thereby casing 7 can pass guide way 9 and make mar piece hang on guide way 9 through limiting plate 5 butt guide way 9.

The cover plate 8 and the scratch column 2 are made of polytetrafluoroethylene, can be reused after being sterilized, and have no damage to cells.

The outside one end that casing 7 is close to mar post 2 is equipped with helmet 1, during the use, opens helmet 1, when not using, covers helmet 1 at casing 7 one end, keeps the inside cleanness of mar post 2 of casing 7.

The periphery of the support 6 is provided with an elastic part 3, the elastic part 3 is made of elastic materials such as rubber, and the elastic part 3 is positioned inside the shell 7 and is contacted with the inner wall of the shell 7. Casing 7 is the cuboid form, and support 6 can be cuboid form or cylindrical, and support 6 periphery contacts with casing 7 inner wall through elastic component 3, and elastic component 3 fixes in support 6 periphery, and the cross-section of elastic component 3 periphery is the same with the inside cross-sectional area of casing 7, and that is elastic component 3 is full of the inside cross-section of casing 7 except that support 6 completely, and the cross-section of mar post 2 equals or slightly is less than the inside cross-section of casing 7. During the use, handheld handle 4 pushes down gently, and support 6 drives relative casing 7 downstream of

mar post

2, and 6 peripheries of support are equipped with elastic component 3, and elastic component 3 is together driven downstream, and elastic component 3 plays certain frictional action, makes certain frictional force have between support 6 and the 7 inner walls of casing, and elastic component 3 plays the effect of stabilizing support 6 simultaneously, and mar post 2 trembles when preventing to rule, and the straight line of drawing is very straight.

The operation method of the cell scratching device of the embodiment comprises the following steps:

(1) the cover plate is covered on the disposable six-hole plate, the protective cap is opened, the shell penetrates through the guide groove, the scratch part is suspended on the guide groove under the action of the limiting plate, the height of the bottom of the shell from the bottom surface of the six-hole plate is larger than that of the culture solution in the six-hole plate, and the distance of the bottom of the shell from the height of the liquid in the six-hole plate is 2-3 mm.

(2) When cells in each hole site of the disposable six-hole plate grow to a proper scratching condition, the handle is held by a hand and pushed downwards slightly, the scratching column can be released, and when the scratching column is pushed downwards to be tightly attached to the bottom surfaces of the hole sites on the six-hole plate, the scratching piece is pushed forwards along the guide groove, so that the scratching operation can be completed quickly; after the scribing is finished, the handle is quickly lifted upwards, and the handle drives the support and the scribing column to be lifted upwards.

(3) And (3) cleaning the cells in each hole on the disposable six-hole plate twice by using sterile PBS, adding a culture medium required for growth, simultaneously adding medicines required for experiments, and photographing and recording scratches for the first time under a microscope.

(4) After culturing for a certain time, photographing and recording at the first photographing site.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the utility model has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. The utility model provides a cell mar device, a serial communication port, includes apron and mar piece, be equipped with the guide way on the apron, mar piece includes support and casing, the mar post is connected to the one end of support, the handle is connected to the other end of support, the inside cavity of casing, the mar post with the support is located the casing is inside and can be followed the inside round trip movement of casing, the outer wall of casing is equipped with the limiting plate, the casing can pass the guide way hang in on the guide way.

2. The cell scarification apparatus according to claim 1, wherein the end of the scarification column near the support is rectangular, and the end of the scarification column near the housing is arc-shaped.

3. The cell streaking device as in claim 2, wherein the housing has a rectangular parallelepiped shape, and a width of the housing is the same as a groove width of the guide groove.

4. The cell scarification apparatus according to claim 1, wherein the support is provided with an elastic member at the periphery thereof, the elastic member being located inside the housing and contacting the inner wall of the housing.

5. The cell streaking device as in claim 1, wherein the housing is externally provided with a protective cap near one end of the streaking column.

6. The cell streaking device as in claim 1, wherein the cover plate is identical in shape and size to the six-well plate.

7. The cell streaking device as in claim 6, wherein the guide groove is located at a position of the cover plate corresponding to the hole on the six-hole plate, and the length of the guide groove is the same as the diameter of the hole.

8. The cell streaking device as in claim 6, wherein the cover plate is covered on the six-well plate, and the height of the bottom of the housing from the bottom surface of the six-well plate is greater than the height of the culture solution in the six-well plate.

9. The cell streaking device as in claim 8, wherein the height of the bottom of the housing from the culture solution in the six-well plate is 2-3 mm.

10. The cell streaking device as in claim 1, wherein the cover plate and the streaking post are made of teflon.