CN216005825U - Cell scraper - Google Patents
Cell scraper Download PDFInfo
- Publication number
- CN216005825U CN216005825U CN202122469674.4U CN202122469674U CN216005825U CN 216005825 U CN216005825 U CN 216005825U CN 202122469674 U CN202122469674 U CN 202122469674U CN 216005825 U CN216005825 U CN 216005825U
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- China
- Prior art keywords
- rod body
- plate
- fixed plate
- clamping
- cell scraper
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- 238000011010 flushing procedure Methods 0.000 claims description 11
- 230000007246 mechanism Effects 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 3
- 230000000149 penetrating effect Effects 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 2
- 238000004113 cell culture Methods 0.000 abstract description 30
- 238000006748 scratching Methods 0.000 abstract description 9
- 230000002393 scratching effect Effects 0.000 abstract description 9
- 230000003044 adaptive effect Effects 0.000 abstract description 4
- 238000002474 experimental method Methods 0.000 abstract description 3
- 230000001464 adherent effect Effects 0.000 abstract description 2
- 238000003306 harvesting Methods 0.000 abstract description 2
- 238000005406 washing Methods 0.000 description 5
- 230000009471 action Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 238000007790 scraping Methods 0.000 description 2
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- 238000012832 cell culture technique Methods 0.000 description 1
- 238000011072 cell harvest Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 102000038379 digestive enzymes Human genes 0.000 description 1
- 108091007734 digestive enzymes Proteins 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000003364 immunohistochemistry Methods 0.000 description 1
- 210000001503 joint Anatomy 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a cell scraper, which comprises a first rod body, a second rod body and a scraper blade, wherein the first rod body is provided with a grab handle; the second rod body is hinged with one end of the first rod body into a whole; the fixed plate is a flexible plate body and is fixedly connected to the outer end of the second rod body; and the curvature adjusting part is arranged on one side of the fixed plate and used for drawing the left and right side edges of the fixed plate to bend the fixed plate into an arc-shaped structure. The utility model has the advantages that the angle of the fixing plate connected on the second rod body can be easily adjusted, so that the scratching device which is more adaptive to the attaching scrapes the cells growing on the adherent wall on the cell culture device; the curvature of the fixing plate can be adjusted as required, the fixing plate can be easily switched between the straight plate structure and the arc plate structure, accordingly, the cell culture device with the round hole-shaped structure and the cell culture device with the square structure can be adapted, the adaptability is enhanced, meanwhile, the residual dead angle generated when scratching cells is effectively reduced, the cells on the cell culture device can be scratched completely, and the cell harvesting rate of cell culture experiments is improved.
Description
Technical Field
The utility model belongs to the technical field of laboratory cell culture technique and specifically relates to a cell scraper blade of curvature is adjusted according to cell culture device's shape to convenience.
Background
In basic research, cells are often used as the subject of experimental study, and the cells are placed in a cell culture device (such as a cell culture dish, a cell culture plate, etc.) and cultured under certain experimental conditions, and then the changes of proteins, RNAs, etc. in the cells are observed.
In cell culture experiments, cells usually grow adherently in the culture device, and for further research (such as immunohistochemistry, Western blot, etc.), the cells need to be digested by digestive enzymes, scraped by a cell scraper, washed, and collected. At present, the scraper blade on the market is simple in structure, is a handle usually, and the front end is provided with a scraper blade, directly scratches in the cell culture device bottom, but present cell culture device shape is different and inconsistent in size, has round hole shape, square etc. and present cell scraper blade is the flat board, can't change the curvature according to the cell culture device of different shapes when scratching, is difficult to scratch cleanly, remains the dead angle easily, leads to the cell rate of recovery to descend.
Disclosure of Invention
An object of the utility model is to provide a cell scraper blade, this cell scraper blade can be according to cell culture device's shape and adjust the curvature, remain the dead angle when avoiding scratching the cell.
In order to achieve the above purpose, the utility model can adopt the following technical proposal:
cell scraper blade, include:
the first rod body is provided with a grab handle;
the second rod body is hinged with one end of the first rod body into a whole;
the fixed plate is a flexible plate body and is fixedly connected to the outer end of the second rod body; and
and the curvature adjusting part is arranged on one side of the fixed plate and used for drawing the left and right side edges of the fixed plate to bend the fixed plate into an arc-shaped structure.
Furthermore, the curvature adjusting part comprises a first adjusting screw rod vertically fixedly connected to the middle part of the upper edge of the fixed plate, a push-pull plate is slidably sleeved on the first adjusting screw rod, a pair of horizontal diagonal ribs is fixedly connected to the push-pull plate, the outer ends of the two horizontal diagonal ribs are fixedly connected to the middle parts of the left and right side edges of the fixed plate respectively, and a first pushing nut which pushes the push-pull plate outwards is screwed on the first adjusting screw rod between the fixed plate and the push-pull plate.
Furthermore, the other side wall of the fixed plate, which is opposite to the curvature adjusting part, is provided with two groups of clamping parts, the two groups of clamping parts are symmetrically arranged at the edges of the left side and the right side of the fixed plate, and two groups of clamping parts clamp a flexible scraper plate with clamping openings facing downwards and jointly.
Furthermore, the clamping part comprises a second adjusting screw rod vertically penetrating and fixed on the side wall of the fixing plate, a clamping plate is slidably sleeved on the second adjusting screw rod, and a second pushing nut for pushing the clamping plate is screwed on the second adjusting screw rod positioned on the outer side of the clamping plate; and a rubber anti-skid pad is arranged on the clamping surface of the clamping plate.
Further, the grab handle is positioned at the end, far away from the hinge, of the first rod body; the outer wall of the grab handle is an anti-skid rough surface.
Furthermore, a flushing pipeline is embedded in the fixed plate, and a liquid outlet port of the flushing pipeline is positioned at the center of the lower edge of the side wall of the fixed plate at the same side as the curvature adjusting part; the flushing pipeline sequentially penetrates through the fixed plate, the second rod body and the first rod body, penetrates out from the tail end of the grab handle in a sealing mode, and is communicated with external flushing equipment.
Furthermore, a locking mechanism for locking the rotation angle is arranged at the hinged position of the first rod body and the second rod body.
The utility model has the advantages that the angle of the fixed plate connected on the second rod body can be easily adjusted through the first rod body and the second rod body which are hinged with each other, thereby scratching the cells growing on the cell culture device adherent in a more adaptive way; in addition, the curvature adjusting part arranged on one side of the fixed plate can adjust the curvature of the fixed plate as required, so that the fixed plate can be easily switched between a straight plate structure and an arc plate structure, the cell culture device with a round hole-shaped structure and the cell culture device with a square structure can be adapted, the adaptability is enhanced, meanwhile, the residual dead angle generated when scratching cells is effectively reduced, the cells on the cell culture device can be scratched completely, and the cell harvesting rate of a cell culture experiment is improved.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
It should be noted that all the directional indicators (such as upper, lower, left, right, front and rear … …) in the embodiment of the present invention are only used to explain the relative position relationship between the components, the motion situation, etc. in a specific posture (as shown in the drawings), and if the specific posture is changed, the directional indicator is changed accordingly.
In addition, descriptions in the present application as to "first", "second", and the like are for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicit to the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature.
As shown in FIG. 1, the cell scraper of the present invention comprises a first rod 1, a second rod 2, a fixing plate 3 and a curvature adjusting part 4.
Wherein, the first rod body 1 is provided with a grab handle 5, and the second rod body 2 is hinged with one end of the first rod body 1 into a whole. Specifically, grab handle 5 should be located keeping away from the hinge end of first body of rod 1 to set up the outer wall of grab handle 5 into anti-skidding mat surface, so that operating personnel can increase frictional force when the gripping. In addition, the first rod body 1 and the second rod body 2 can be hinged into a whole in the form of a hinge shaft and a hinge lug, namely, the hinge shaft is connected with the first rod body 1, and the hinge lug is connected with the second rod body 2, so that the first rod body 1 and the second rod body 2 are rotatably connected together under the hinging action of the hinge shaft and the hinge lug; at this time, a locking mechanism for locking the rotation angle can be arranged at the hinged position of the first rod body 1 and the second rod body 2, the locking mechanism can be a puller bolt arranged at one end of a hinge shaft, and the hinge shaft and a hinge lug are pressed tightly by screwing the puller bolt, so that the rotation angle of the second rod body 2 is locked; certainly, the locking mechanism can also be a plurality of clamping bulges uniformly distributed on the hinge shaft along the circumferential direction and clamping grooves which are arranged on the hinge lugs and are in adaptive clamping with the clamping bulges, and the rotation angle of the second rod body 2 is locked by the adaptive clamping of the clamping bulges and the clamping grooves, so that the second rod body 2 rotates by a fixed angle each time; in addition, the hinge shaft and the hinge lug can be tightly hinged together, so that the second rod body 2 can be kept at the angle to scratch cells after the second rod body 2 is forcibly rotated.
The fixed plate 3 is a flexible plate body of a rectangular structure which can be bent and is fixedly connected with the outer end of the second rod body 2 (namely the other end opposite to the hinged end of the first rod body 1), and the lower edge of the fixed plate 3 is perpendicular to the axis of the second rod body 2.
The curvature adjusting part 4 is disposed on one side of the fixing plate 3 and is used for pulling the left and right edges of the fixing plate 3 to bend the fixing plate into an arc structure. Specifically, the curvature adjusting part 4 comprises a first adjusting screw rod 4.1 vertically fixedly connected to the middle part of the upper edge of the fixed plate 3, a push-pull plate 4.2 is slidably sleeved on the first adjusting screw rod 4.1, a pair of horizontal diagonal braces 4.3 is fixedly connected to the push-pull plate 4.2, the outer ends of the two horizontal diagonal braces 4.3 are fixedly connected to the middle parts of the left and right edges of the fixed plate 3 respectively, and a first pushing nut 4.4 for pushing the push-pull plate 4.2 outwards is screwed on the first adjusting screw rod 4.1 between the fixed plate 3 and the push-pull plate 4.2; of course, the horizontal diagonal draw bars 4.3 can be connected with the fixed plate 3 in a hinged mode, so that the fixed plate 3 can be pulled to rotate at a certain angle, and the rigid distortion of the horizontal diagonal draw bars 4.3 in the pulling process is reduced.
In order to prevent the cross contamination when scraping different cells, the other side wall of the fixing plate 3 opposite to the curvature adjusting part 4 can be provided with two groups of clamping parts 6 which are symmetrically arranged at the left and right side edges of the fixing plate 3, and the clamping openings of the two groups of clamping parts 6 face downwards and jointly clamp a disposable flexible scraper 7; specifically, the clamping part 6 comprises a second adjusting screw 6.1 vertically penetrating and fixed on the side wall of the fixing plate 3, a clamping plate 6.2 is slidably sleeved on the second adjusting screw 6.1, and a second pushing nut 6.3 for pushing the clamping plate 6.2 is screwed on the second adjusting screw 6.1 positioned at the outer side of the clamping plate 6.2; in order to improve the clamping force of the clamping part 6, a layer of rubber anti-slip mat 6.4 can be arranged on the clamping surface of the clamping plate 6.2. The flexible scraper 7 can be bent into an arc shape under the action of the curvature adjusting part 4 along with the fixed plate 3 under the clamping of the clamping part 6, so that the flexible scraper can be adapted to a cell culture dish with a round hole-shaped structure, cells on the cell culture dish can be scraped more cleanly, residual dead corners are avoided, and the cell recovery rate is improved; meanwhile, after scratching the cells on one cell culture dish, the flexible scraper 7 can be quickly detached, and the flexible scraper 7 can be replaced when scratching the cells on the next cell culture dish, so that the cell pollution is effectively prevented.
In addition, still need frequent washing attached to the cell culture dish when scratching the cell, thereby make the cell drop and be convenient for scratched, consequently, still bury a washing pipeline 8 in fixed plate 3, this washing pipeline 8's play liquid port is located fixed plate 3 lateral wall lower edge center department with curvature adjusting part 4 homonymy, the feed liquor port of washing pipeline 8 then should pass fixed plate 3, second body of rod 2 and first body of rod 1 in proper order to seal the wearing out from the tail end of grab handle 5, thereby can be light with outside flushing device (like washing needle tubing, flush pump etc.) butt joint intercommunication.
When the device is used, the angle of the second rod body 2 is adjusted as required, the flexible scraper 7 is clamped on the lower edge of the fixed plate 3 through the clamping part 6, the curvature adjusting part 4 is adjusted according to the shape of a cell culture dish to be scratched, the curvature adjusting part 4 is not adjusted if the cell culture dish is square, and the first pushing nut 4.4 is rotated if the cell culture dish is round, so that the first pushing nut 4.4 pushes the push-pull plate 4.2 outwards, the fixed plate 3 is bent into an arc under the action of the two horizontal diagonal ribs 4.3, the flexible scraper 7 clamped on the clamping part 6 is bent into an arc along with the fixed plate 3 and is matched with the cell culture dish with a round structure, cells on the cell culture dish can be scratched completely, and residual dead angles generated when the cells are scratched are avoided; in addition, scratch the in-process of scraping the cell, still can be connected the inlet port that washes pipeline 8 with outside flushing device to continuous dropwise add flush fluid on coming to the cell culture dish through flushing device, make the cell scratch cleaner, improve the cell harvest rate greatly.
Claims (10)
1. A cell scraper, comprising: comprises that
The first rod body is provided with a grab handle;
the second rod body is hinged with one end of the first rod body into a whole;
the fixed plate is a flexible plate body and is fixedly connected to the outer end of the second rod body; and
and the curvature adjusting part is arranged on one side of the fixed plate and used for drawing the left and right side edges of the fixed plate to bend the fixed plate into an arc-shaped structure.
2. The cell scraper of claim 1, wherein: the curvature adjusting part comprises a first adjusting screw rod vertically and fixedly connected to the middle of the upper edge of the fixed plate, a push-pull plate is slidably sleeved on the first adjusting screw rod, a pair of horizontal diagonal ribs are fixedly connected to the push-pull plate, the outer ends of the two horizontal diagonal ribs are fixedly connected to the middle parts of the left side edge and the right side edge of the fixed plate respectively, and a first pushing nut which pushes the push-pull plate outwards is screwed on the first adjusting screw rod between the fixed plate and the push-pull plate.
3. The cell scraper of claim 1, wherein: and the other side wall of the fixed plate, which is opposite to the curvature adjusting part, is provided with two groups of clamping parts, the two groups of clamping parts are symmetrically arranged at the edges of the left side and the right side of the fixed plate, and two groups of clamping parts clamp a flexible scraper plate together with a downward clamping opening.
4. The cell scraper of claim 3, wherein: the clamping part comprises a second adjusting screw rod vertically penetrating and fixed on the side wall of the fixing plate, a clamping plate is sleeved on the second adjusting screw rod in a sliding mode, and a second pushing nut used for pushing the clamping plate is connected to the second adjusting screw rod located on the outer side of the clamping plate in a threaded mode.
5. The cell scraper of claim 4, wherein: and a rubber anti-skid pad is arranged on the clamping surface of the clamping plate.
6. The cell scraper of claim 1, wherein: the grab handle is positioned at the end, far away from the hinge, of the first rod body.
7. The cell scraper of claim 6, wherein: the outer wall of the grab handle is an anti-skid rough surface.
8. The cell scraper of claim 6, wherein: a flushing pipeline is embedded in the fixed plate, and a liquid outlet port of the flushing pipeline is positioned at the center of the lower edge of the side wall of the fixed plate at the same side as the curvature adjusting part.
9. The cell scraper of claim 8, wherein: the flushing pipeline sequentially penetrates through the fixed plate, the second rod body and the first rod body, penetrates out from the tail end of the grab handle in a sealing mode, and is communicated with external flushing equipment.
10. The cell scraper of claim 1, wherein: and a locking mechanism for locking the rotation angle is arranged at the hinged position of the first rod body and the second rod body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122469674.4U CN216005825U (en) | 2021-10-14 | 2021-10-14 | Cell scraper |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122469674.4U CN216005825U (en) | 2021-10-14 | 2021-10-14 | Cell scraper |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216005825U true CN216005825U (en) | 2022-03-11 |
Family
ID=80522136
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202122469674.4U Active CN216005825U (en) | 2021-10-14 | 2021-10-14 | Cell scraper |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216005825U (en) |
-
2021
- 2021-10-14 CN CN202122469674.4U patent/CN216005825U/en active Active
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| GR01 | Patent grant | ||
| GR01 | Patent grant |