CN215986089U - Cooling device for immunoblot test electrotransformation - Google Patents

Cooling device for immunoblot test electrotransformation Download PDF

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Publication number
CN215986089U
CN215986089U CN202122250002.4U CN202122250002U CN215986089U CN 215986089 U CN215986089 U CN 215986089U CN 202122250002 U CN202122250002 U CN 202122250002U CN 215986089 U CN215986089 U CN 215986089U
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China
Prior art keywords
drawer
box body
placing frame
groove
sliding plate
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CN202122250002.4U
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Chinese (zh)
Inventor
赵敬会
王炼
蔡云
高君伟
杨玲
邹娇
刘天曜
朱志茹
范晓棠
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Third Military Medical University TMMU
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Third Military Medical University TMMU
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Abstract

The utility model relates to the technical field of electric rotary tanks, in particular to an electric rotary tank cooling device for an immunoblot test, which comprises a tank body, wherein a first placing rack and a second placing rack are respectively and fixedly arranged on the inner walls of two sides of the tank body, an electric rotary tank is arranged between the first placing rack and the second placing rack, a mounting groove is formed in the tank body, a drawer is arranged in the mounting groove, elastic parts are respectively arranged on two sides of the drawer, a fixing part is arranged on one side of the drawer, a hose is fixedly arranged on the drawer, a one-way valve is fixedly arranged on the hose, a temperature sensor, an infrared temperature detector and a temperature display are fixedly arranged on the drawer, the temperature display, the temperature sensor and the infrared temperature detector are electrically connected, and the electric rotary tank cooling device is used for solving the problem that the cooling effect of ice water cannot be visually observed in the long-time electric rotary process by a cooling mode of embedding the electric rotary tank into the ice water in the electric rotary process at present, and when the ice water is added, the problem that the ice water after being heated is complicated to replace is solved.

Description

Cooling device for immunoblot test electrotransformation
Technical Field
The utility model relates to the technical field of electrotransfer tanks, in particular to a cooling device for electrotransfer of an immunoblot test.
Background
Western Blot. It is a commonly used experimental method in molecular biology, biochemistry and immunogenetics. The basic principle is to stain a gel electrophoresis treated cell or biological tissue sample with a specific antibody. Information on the expression of a specific protein in the analyzed cell or tissue is obtained by analyzing the location and depth of staining. In the membrane transferring process of the immunoblotting wet transfer method, as the current is large and the electricity transfer time is long, the electricity transfer liquid and the electricity transfer groove can rise to high temperature, the membrane transferring efficiency and the service life of the electricity transfer instrument are influenced, and therefore, the electricity transfer groove can be generally buried in ice water for cooling. However, the electric rotating tank is embedded in ice water for cooling, so that the ice water cooling effect cannot be visually observed in the long-time electric rotating process, and when ice water is added, the heated ice water needs to be discharged to ensure the refrigeration effect of the ice water, so that the operation is complicated, and the efficiency is low.
SUMMERY OF THE UTILITY MODEL
In view of the above, the present invention provides a temperature reduction device for immunoblotting test electric transfer, which is used to solve the problems that in the conventional electric transfer process, the temperature reduction effect of ice water cannot be visually observed in a long-time electric transfer process by embedding an electric transfer groove in ice water, and the replacement of the heated ice water is complicated when the ice water is added.
The utility model solves the technical problems by the following technical means:
a cooling device for electric transfer of immunoblot tests comprises a box body, wherein handles are fixedly arranged on the outer walls of two sides of the box body, a first placing frame and a second placing frame are fixedly arranged on the inner walls of two sides of the box body respectively, an interval is arranged between the first placing frame and the second placing frame, an electric transfer groove is arranged between the first placing frame and the second placing frame, a mounting groove is formed in the box body, a drawer is mounted in the mounting groove, cavities are formed in two sides of the drawer and two sides of the box body respectively, elastic parts are mounted on two sides of the drawer, a fixing part is arranged on one side of the drawer, a hose is fixedly arranged on the drawer, the hose penetrates through the box body, a one-way valve is fixedly arranged on the hose, a pulling groove is formed in the drawer, a temperature sensor, an infrared temperature detector and a temperature display are fixedly arranged on the drawer, the temperature display is electrically connected with the temperature sensor and the infrared temperature detector.
Further, first rack and second rack structure are the same, first rack is L shape, the fixed baffle that is provided with on the first rack, a plurality of first through-holes have been seted up on the first rack, a plurality of second through-holes have been seted up on the baffle, set up like this, make the ice salt in the drawer when melting the cooling, can make the electric runner cool down under the condition of contactless ice salt water, and make the electric runner contact with more air conditioning.
Furthermore, a notch is formed in the first placing frame, so that the electric rotary groove can be conveniently taken and placed.
Furthermore, the elastic part includes sliding plate, guide arm and first spring, the sliding plate is L shape, the one end of sliding plate is installed on the drawer, the spiro union has the bolt on the sliding plate, the sliding plate is located the cavity, the guide arm is installed in the box, and wears to produce the sliding plate, first spring housing is established on the guide arm, the one end fixed connection of first spring is on the inner wall of box, and other end fixed connection is on the sliding plate, sets up like this, and when making the brine ice in the change drawer, the drawer can pop out one section distance automatically, is convenient for directly add the ice salt mixture.
Further, the mounting is including rotating post, turning block, fixed block, joint pole and second spring, it is T shape to rotate the post, it is on the drawer to rotate post fixed mounting, the one end of turning block is rotated and is installed on rotating the post, fixed block fixed mounting is on the box, the joint groove has been seted up on the fixed block, the joint pole is T shape, the joint pole is worn to establish on the fixed block, the second spring cup joints on the joint pole, the one end fixed connection of second spring is on the fixed block, and other end fixed connection is terminal at the joint pole, sets up like this, makes the drawer when not using, can accomodate in the box completely, avoids taking up more storage area.
Furthermore, the position of the mounting groove on the box body is provided with a sealing groove, and the drawer is fixedly provided with a sealing ring, so that when the drawer is stored in the box body, the cold air is prevented from leaking from the mounting groove when the ice salt is refrigerated, and the refrigerating effect in the box body is improved.
According to the cooling device for the electrokinetic transfer of the immunoblot test, the first placing frame and the second placing frame are arranged in the box body, so that the electrokinetic transfer tank can be cooled under the condition of not contacting ice brine, and the trouble that the outer wall of the electrokinetic transfer tank needs to be wiped dry after the electrokinetic transfer is finished is avoided; through set up the drawer in the box, be provided with temperature-sensing ware and temperature monitor on the drawer, make the electricity change groove at long-time electricity change in-process, the temperature of ice water can audio-visually observed, the temperature of cooperation infrared thermoscope, the cooling effect of ice water of observation that can be direct-viewing, and through mutually supporting of mounting, check valve and elastic component, the ice water that no longer has the refrigeration effect of change that can be timely, convenient, swift.
Drawings
FIG. 1 is a schematic structural diagram of a cooling device for electrotransfer of an immunoblot assay according to the present invention;
FIG. 2 is a schematic sectional view of a cooling device for an immunoblot assay electrotransformation, according to the present invention;
FIG. 3 is an enlarged schematic view of the structure at A in FIG. 2;
FIG. 4 is a schematic sectional view of a cooling device for an immunoblot assay electrotransformation, according to a second embodiment of the present invention;
FIG. 5 is an enlarged schematic view of the structure at B in FIG. 4;
FIG. 6 is a schematic view of an installation structure of a cooling device for an immunoblot assay electrotransformation, according to the present invention;
the temperature display device comprises a box body 1, a cavity 101, a handle 11, a mounting groove 12, a sealing groove 121, a hose 13, a check valve 131, a first placing frame 2, a first through hole 201, a second placing frame 21, a notch 22, a baffle 23, a second through hole 231, an electric rotating groove 3, a drawer 4, a sliding plate 41, a bolt 411, a guide rod 42, a first spring 421, a sealing ring 43, a rotating column 44, a rotating block 441, a fixing block 442, a clamping groove 443, a clamping rod 444, a second spring 445, a pulling groove 45 and a temperature display 5.
Detailed Description
The utility model will be described in detail below with reference to the following figures and specific examples:
as shown in fig. 1 to 6, the cooling device for the electrokinetic transfer of the immunoblot test comprises a box body 1, wherein handles 11 are fixedly arranged on the outer walls of two sides of the box body 1, so that the whole cooling device is convenient to carry. The first placing frame 2 and the second placing frame 21 are fixedly arranged on the inner walls of the two sides of the box body 1 respectively, an interval exists between the first placing frame 2 and the second placing frame 21, the electric rotary groove 3 is placed between the first placing frame 2 and the second placing frame 21, when the electric rotary groove 3 is placed between the first placing frame 2 and the second placing frame 21, the air conditioning can contact the electric rotary groove 3 in the largest area, and the electric rotary groove 3 achieves the largest cooling effect. The box body 1 is provided with the mounting groove 12, the drawer 4 is mounted in the mounting groove 12, ice salt is arranged in the drawer 4, some salt is scattered on the solid ice blocks, two times of heat absorption can be formed, and the cooling effect can be better. Fixed temperature-sensing ware that is provided with on drawer 4, infrared thermoscope and temperature monitor 5, temperature monitor 5 and temperature-sensing ware and the equal electricity of infrared thermoscope are connected, temperature-sensing ware is used for the temperature of real-time supervision brine ice, infrared thermoscope is used for monitoring the temperature of electricity inverter groove 3, the temperature real-time feedback through the temperature with brine ice and the temperature of electricity inverter groove 3 is on temperature monitor 5, be convenient for audio-visual cooling effect of observing brine ice, and observe the real-time temperature of brine ice, be convenient for in time change the brine ice that can not refrigerate. The fixed hose 13 that is provided with on the drawer 4, box 1 is worn to establish out by hose 13, the fixed check valve 131 that is provided with on the hose 13, when making the change brine ice, directly open check valve 131 can be discharged brine ice, is convenient for put into drawer 4 with new solid brine ice mixture, and is convenient, swift.
The first placing frame 2 and the second placing frame 21 are identical in structure, the first placing frame 2 is L-shaped, and a baffle 23 is fixedly arranged on the first placing frame 2, so that the whole first placing frame and the whole second placing frame are Z-shaped. Seted up a plurality of first through-holes 201 on the first rack 2, seted up a plurality of second through-holes 231 on the baffle 23, under the cooperation of Z shape rack and first through-hole 201 and second through-hole 231, make the ice salt in the drawer 4 when melting the cooling, can make electric runner 3 cool down under the condition of contactless ice salt water, and make electric runner 3 can contact with more air conditioning, promote the cooling effect. The first placing frame 2 is provided with a notch 22, and the electric rotating groove 3 is convenient to take and place through the notch 22.
Cavity 101 all exists in the both sides of drawer 4 and the both sides of box 1, and the both sides of drawer 4 are located cavity 101 and all are installed the elastic component, through the setting of elastic component, and when making the brine ice that needs to be changed in the drawer 4, after the brine ice that will no longer have refrigeration effect through check valve 131 discharges, drawer 4 can one section distance by the auto-eject, is convenient for directly add the brine ice mixture. The elastic member includes a sliding plate 41, a guide rod 42 and a first spring 421, the sliding plate 41 is L-shaped, one end of the sliding plate 41 is installed on the drawer 4, and a bolt 411 is screwed on the sliding plate 41, so as to facilitate installation of the sliding plate 41. Sliding plate 41 is located cavity 101, guide arm 42 is installed in box 1, and wear to establish sliding plate 41, first spring 421 cover is established on guide arm 42, the one end fixed connection of first spring 421 is on the inner wall of box 1, other end fixed connection is on sliding plate 41, when making drawer 4 remove in mounting groove 12, sliding plate 41 removes along guide arm 42, avoid drawer 4 screens on the one hand, on the other hand, when making drawer 4 not use, the effect of deformation is resumeed to first spring 421, can pop out drawer 4 one section distance of box, be convenient for add the ice salt mixture.
The drawer 4 is provided with a pulling groove 45, so that a user can push the drawer 4 with the replaced ice-salt mixture conveniently. One side of drawer 4 is provided with the mounting, through the setting of mounting, makes drawer 4 when not using, can accomodate in box 1 completely, avoids taking up more storage area, simultaneously, can further fix drawer 4 in box 1, when avoiding cooling, the unexpected roll-off of drawer 4 leads to the cooling effect to descend.
The fixing member includes a rotating post 44, a rotating block 441, a fixing block 442, a clamping rod 444 and a second spring 445, the rotating post 44 is T-shaped, the rotating post 44 is fixedly installed on the drawer 4, and one end of the rotating block 441 is rotatably installed on the rotating post 44, so that the rotating block 441 can rotate relative to the rotating post 44 without slipping. The fixing block 442 is fixedly mounted on the case 1, and a clamping groove 443 is formed in the fixing block 442, so that the rotating block 441 can be clamped in the clamping groove 443. Clamping rod 444 is T shape, and clamping rod 444 wears to establish on fixed block 442, and second spring 445 cup joints on clamping rod 444, and the one end fixed connection of second spring 445 is on fixed block 442, and other end fixed connection is at clamping rod 444 end, makes clamping rod 444 can further restrict the position of turning block 441, guarantees that drawer 4 stably accomodates in box 1. The sealing groove 121 is formed in the position of the mounting groove 12 on the box body 1, the sealing ring 43 is fixedly arranged on the drawer 4, and through the arrangement of the sealing ring 43 and the sealing groove 121, when the drawer 4 is stored in the box body 1, the phenomenon that ice salt is refrigerated is avoided, cold air leaks from the mounting groove 12, and the refrigerating effect in the box body 1 is improved.
The using method of the utility model is as follows: when the ice salt storage box is used, the clamping rod 444 is pulled to enable the rotating block 441 to rotate out of the clamping groove 443, the drawer 4 slides out a distance relative to the box body 1 under the action of the two first springs 421, ice salt mixture is placed into the drawer 4, the drawer 4 is pushed to slide along the installation groove 12 in the opposite direction, the sealing ring 43 abuts against the sealing groove 121, the clamping rod 444 is pulled to enable the rotating block 441 to rotate into the clamping groove 443, the clamping rod 444 is loosened to limit the position of the rotating block 441, in the process, the sliding plate 41 is driven to move along the guide rod 42 by the movement of the drawer 4, the first springs 421 restore to deform firstly and then are compressed, the hoses 13 firstly enter the installation groove 12 for a distance and then restore to the original shape; put the electricity transfer groove 3 between first rack 2 and second rack 21 again, at the in-process that begins the electricity transfer to begin refrigeration, cooling, through mutually supporting of temperature-sensing ware and infrared thermoscope and temperature display 5, can audio-visual observation brine ice's cooling effect, and observe brine ice's temperature, be convenient for in time change the brine ice that can not refrigerate.
When the brine ice that no longer has refrigeration effect needs to be changed, open check valve 131, discharge the brine ice this moment, pulling joint pole 444, rotate turning block 441, make turning block 441 break away from joint groove 443, under the effect of two first springs 421, drawer 4 pops out one section distance for box 1 is automatic relative, is convenient for put into drawer 4 with new brine ice mixture, repeats above-mentioned step, can cool down electric rotary trough 3 once more, and is convenient, swift.
Although the present invention has been described in detail with reference to the preferred embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted without departing from the spirit and scope of the utility model as defined in the appended claims. The techniques, shapes, and configurations not described in detail in the present invention are all known techniques.

Claims (6)

1. The utility model provides a heat sink is used in experimental electricity of immunoblotting which characterized in that: comprises a box body, handles are fixedly arranged on the outer walls of two sides of the box body, a first placing frame and a second placing frame are respectively and fixedly arranged on the inner walls of two sides of the box body, a space is arranged between the first placing rack and the second placing rack, an electric rotary groove is arranged between the first placing rack and the second placing rack, the box body is provided with an installation groove, a drawer is arranged in the installation groove, cavities are arranged on both sides of the drawer and both sides of the box body, elastic pieces are arranged on both sides of the drawer, a fixing piece is arranged on one side of the drawer, a hose is fixedly arranged on the drawer, the hose penetrates through the box body, a one-way valve is fixedly arranged on the hose, a pulling groove is arranged on the drawer, the drawer is fixedly provided with a temperature sensor, an infrared temperature detector and a temperature display, and the temperature display is electrically connected with the temperature sensor and the infrared temperature detector.
2. The cooling device for the electrotransfer of the immunoblot assay according to claim 1, wherein: the first placing frame and the second placing frame are identical in structure, the first placing frame is L-shaped, a baffle is fixedly arranged on the first placing frame, a plurality of first through holes are formed in the first placing frame, and a plurality of second through holes are formed in the baffle.
3. The cooling device for the electrotransfer of the immunoblot assay according to claim 2, wherein: a notch is formed in the first placing frame.
4. The cooling device for the electrotransfer of the immunoblot assay according to claim 3, wherein: the elastic piece comprises a sliding plate, a guide rod and a first spring, the sliding plate is L-shaped, one end of the sliding plate is installed on the drawer, a bolt is screwed on the sliding plate, the sliding plate is located in the cavity, the guide rod is installed in the box body and penetrates through the sliding plate, the first spring is sleeved on the guide rod, one end of the first spring is fixedly connected to the inner wall of the box body, and the other end of the first spring is fixedly connected to the sliding plate.
5. The cooling device for the electrotransfer of the immunoblot assay according to claim 4, wherein: the mounting is including rotating post, turning block, fixed block, joint pole and second spring, it is T shape to rotate the post, it is on the drawer to rotate post fixed mounting, the one end of turning block is rotated and is installed on rotating the post, fixed block fixed mounting is on the box, the joint groove has been seted up on the fixed block, the joint pole is T shape, the joint pole is worn to establish on the fixed block, the second spring cup joints on the joint pole, the one end fixed connection of second spring is on the fixed block, and other end fixed connection is terminal at the joint pole.
6. The cooling device for the electrotransfer of the immunoblot assay according to claim 5, wherein: the box body is provided with a sealing groove at the position of the mounting groove, and the drawer is fixedly provided with a sealing ring.
CN202122250002.4U 2021-09-16 2021-09-16 Cooling device for immunoblot test electrotransformation Active CN215986089U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202122250002.4U CN215986089U (en) 2021-09-16 2021-09-16 Cooling device for immunoblot test electrotransformation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202122250002.4U CN215986089U (en) 2021-09-16 2021-09-16 Cooling device for immunoblot test electrotransformation

Publications (1)

Publication Number Publication Date
CN215986089U true CN215986089U (en) 2022-03-08

Family

ID=80463277

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202122250002.4U Active CN215986089U (en) 2021-09-16 2021-09-16 Cooling device for immunoblot test electrotransformation

Country Status (1)

Country Link
CN (1) CN215986089U (en)

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