CN215962239U

CN215962239U - Novel purification column is used in production of liraglutide

Info

Publication number: CN215962239U
Application number: CN202023259563.2U
Authority: CN
Inventors: 钱海; 邱倩倩
Original assignee: Nanjing Novin Haitai Biomedical Co ltd
Current assignee: Nanjing Novin Haitai Biomedical Co ltd
Priority date: 2020-12-30
Filing date: 2020-12-30
Publication date: 2022-03-08
Anticipated expiration: 2030-12-30

Abstract

The utility model discloses a novel purification column for liraglutide production, which is provided with a dispersion screen and can disperse liquid to be purified in a sample introduction process, so that the phenomenon that the upper surface of a filler is uneven and even the filler splashes due to the concentration of liquid entry points in the sample introduction process is avoided, the effective column volume can not be shortened, and the purification effect of a chromatographic column is good; the dispersing sieve and the filter membrane pressing ring are both made of rubber or silica gel, so that the upper filter membrane and the lower filter membrane can be conveniently disassembled and replaced, all elements are detachably connected through threads, the disassembly is simple and convenient, the chromatographic column is more thorough to disassemble and clean, and the cleaning dead angle and the pollution to purified substances are avoided; the upper filter membrane and the lower filter membrane are fixed in a clamping and pressing mode, so that the phenomenon that the upper filter membrane and the lower filter membrane deviate in the use process of the chromatographic column is avoided, the upper filter membrane and the lower filter membrane are fully utilized, the filtration is comprehensive, the filler blockage or filler loss is prevented, the necessary regeneration process time caused by the fact that the filler is blocked by the sample injection liquid is shortened, and the workload of an operator is reduced.

Description

Novel purification column is used in production of liraglutide

Technical Field

The utility model relates to the technical field of purification equipment, in particular to a novel purification column for producing liraglutide.

Background

With the improvement of living standard and the change of life style, the incidence of diabetes in China tends to increase year by year in recent years. Diabetes is mainly characterized by hyperglycemia, and typical cases can show symptoms of polyuria, polydipsia, polyphagia, emaciation and the like, namely symptoms of more than three and one less, once the control is not good, serious complications can be caused, so that exhaustion pathological changes of parts such as kidney, eyes, feet and the like can be caused, and the diseases can not be cured. Diabetes is classified into gestational diabetes, specific diabetes, type I diabetes, and type II diabetes. Among them, type II diabetes, also known as non-insulin dependent diabetes mellitus, is characterized in that the human body can produce insulin, but the cells cannot respond to it, so that the effect of insulin is greatly reduced.

Type II diabetes is mainly treated by oral or subcutaneous injections of hypoglycemic agents. Many hypoglycemic drugs aiming at type II diabetes mellitus comprise metformin, sulfonylureas drugs, receptor kinetin of glucagon-like peptide-1 (GLP-1) and the like, and the receptor kinetin of GLP-1 is a hot point of research in recent years. Among them, liraglutide (liraglutide) is one of GLP-1 receptor agonists, and has a good hypoglycemic effect, and can improve glycemic control by reducing fasting and postprandial blood glucose in type II diabetes patients, and at the same time, can reduce the body weight of patients. Compared with natural GLP-1, the drug effect is equivalent and the action time is longer. Column purification techniques are often used during the purification of liraglutide.

The prior art chromatography columns often have the following problems: firstly, a chromatographic column in the prior art is not easy to remove, when the chromatographic column needs to be removed and cleaned, the chromatographic column is difficult to remove, fewer detachable parts cannot be used for cleaning comprehensively, dirt is often stored in certain dead corners to cause pollution of purified liraglutide, and secondly, when the chromatographic column is used, an upper filter membrane and a lower filter membrane sometimes deviate, an upper filter membrane deviates, a liquid to be purified cannot be filtered comprehensively, packing of the chromatographic column is often blocked, column efficiency is affected, the regeneration operation of the chromatographic column needs to be performed, and time and labor are wasted; the lower filter membrane deflects, and the filler in the chromatographic column leaks out from the deflected gap, so that the effective column volume is reduced, and the purified liquid is polluted. Third, when advancing the appearance, sample solution advances kind from the middle part on chromatographic column top, can make the upper surface unevenness of interior filler of chromatographic column, even makes the cylinder interior filler splash to the inner wall of chromatographic column on, the filler that splashes on the inner wall can not obtain effectual utilization, shortens effective column volume, influences the purification effect.

SUMMERY OF THE UTILITY MODEL

The utility model aims to solve the defects in the prior art and provides a novel purification column for producing liraglutide.

In order to achieve the purpose, the utility model adopts the following technical scheme: the utility model provides a production of liraglutide is with novel purification post, includes hollow column tube, upper end cover and lower end cover, the upper end cover with lower end cover detachable respectively fixes the upper and lower both ends of column tube, be equipped with upper filter membrane and dispersion sieve in the upper end cover, the upper filter membrane sets up the top of dispersion sieve, the fixed first connecting pipe in top of upper end cover, be equipped with lower filter membrane and filter membrane clamping ring in the lower end cover, the fixed second connecting pipe in below of lower end cover.

Preferably, the upper end cover with the lower end cover structure is the same, the inside wall of upper end cover is fixed with the hole sieve, the hole sieve will the upper end cover separate into about two-layer, the middle part of the apron of upper end cover is equipped with connect the via hole, be equipped with the internal thread on connect the via hole's the lateral wall, the downside of first connecting pipe is equipped with the corresponding external screw thread, first connecting pipe passes through threaded connection and fixes on the upper end cover, the upper end cover is kept away from the inside wall of one side of apron is equipped with the installation internal thread, the lateral wall of both sides is equipped with the installation external screw thread that corresponds about the pillar pipe.

Preferably, the dispersion sieve includes dispersion sieve main part and sealing ring, the dispersion sieve main part is hollow round platform form, the dispersion sieve main part includes the upper screen, lower sieve and connects the lateral wall of upper screen and lower sieve, the diameter of upper screen is greater than the diameter of lower sieve, be equipped with the dispersion through-hole on the lateral wall, the sealing ring cover is established the periphery of lateral wall, the upside of sealing ring is connected and is fixed on the lateral wall.

Preferably, the dispersion sieve is made of silica gel or rubber, and under the installation state, the below of utensil hole sieve plate sets up the dispersion sieve, utensil hole sieve with centre gripping between the dispersion sieve goes up the filter membrane, the dispersion sieve main part is followed the upper end of column tube stretches into in the pipe of column tube, the sealing ring cover is established on the lateral wall of column tube.

Preferably, the lower filter membrane is arranged above the sieve plate with the holes on the inner side wall of the lower end cover, and the filter membrane pressing ring is arranged in the inner cavity of the lower end cover and pressed on the lower filter membrane.

Preferably, the first connecting pipe and the second connecting pipe are identical in structure, a sealing cover is fixedly connected to one side, away from the column pipe, of the first connecting pipe through threads, a pipeline channel is arranged on the upper side face of the sealing cover, a sealing block is arranged in the sealing cover, a pipeline hole is formed in the center of the sealing block, and a first groove for the sealing block to be arranged is formed in the first connecting pipe.

Preferably, the sealing block is a circular truncated cone, and the pipeline hole penetrates through the upper surface and the lower surface of the circular truncated cone.

Preferably, the filter membrane pressing ring is made of rubber or silica gel.

Preferably, a cock type valve is arranged on the second connecting pipe.

Compared with the prior art, the utility model has the beneficial effects that: (1) the dispersion sieve and the filter membrane compression ring are made of rubber or silica gel, can be detached from the upper end cover and the lower end cover after certain deformation, and can be detached and replaced, and the upper end cover, the lower end cover, the column tube, the first connecting tube, the second connecting tube, the sealing cover and other elements are connected and detached through threads, so that the detachment is simple and convenient, the chromatographic column is more thorough when detached and cleaned, and the cleaning dead angle is avoided, and the purified substance is polluted; (2) the dispersion sieve is arranged and provided with the upper sieve, the lower sieve and the side wall with the hole, so that liquid to be purified can be dispersed in a sample introduction manner, the phenomenon that the upper surface of the filler is uneven and even the filler splashes due to the concentration of liquid entry points in the sample introduction process is avoided, the effective column volume cannot be shortened, and the purification effect of the chromatographic column is good; (3) utensil hole sieve with centre gripping between the dispersion sieve go up the filter membrane, filter membrane clamping ring pressfitting is in on the filter membrane down, avoided going up the filter membrane and the phenomenon appearance that the filter membrane takes place the skew in the chromatographic column use down, go up the filter membrane and obtain make full use of with lower filter membrane, filter comprehensively, prevent to pack to block up or pack and run off, reduce to block up to lead to the fact the post to need regeneration operation because of the introduction liquid, reduce unnecessary regeneration process time, reduce operator's work load.

Drawings

FIG. 1 is a schematic structural diagram of a novel purification column for producing liraglutide according to the present invention;

FIG. 2 is a sectional view of the A-A column for producing liraglutide according to the present invention;

fig. 3 is a schematic diagram of the three-dimensional structure of the novel purification column dispersion sieve for liraglutide production.

Detailed Description

The present invention will be further described with reference to fig. 1 to 3, but the scope of the present invention should not be limited thereto. For convenience of explanation and understanding of the technical solutions of the present invention, the following descriptions are made with reference to the accompanying drawings.

The utility model provides a novel purification column for liraglutide production, which comprises a hollow column tube 100, wherein a filler is filled in the column tube 100, the filler can be octaalkylsilane bonded silica gel, an upper end cover 200 and a lower end cover 300, the upper end cover 200 and the lower end cover 300 are respectively detachably fixed at the upper end and the lower end of the column tube 100, an upper filter membrane 400 and a dispersion sieve 500 are arranged in the upper end cover 200, the upper filter membrane 400 is arranged above the dispersion sieve 500, a first connecting tube 600 is fixed above the upper end cover 200, a lower filter membrane 700 and a filter membrane pressing ring 800 are arranged in the lower end cover 300, and a second connecting tube 900 is fixed below the lower end cover 300, as shown in figures 1 and 2.

The upper end cover 200 with lower end cover 300 structure is the same, the inside wall of upper end cover 200 is fixed with has hole sieve 210, it will to have hole sieve 210 upper end cover 200 separate into about two-layer, the middle part of upper end cover 200's apron is equipped with connect the through-hole 220, be equipped with the internal thread on connect the through-hole 220's the lateral wall, the downside of first connecting pipe 600 is equipped with the corresponding external screw thread, first connecting pipe 600 is fixed through threaded connection on the upper end cover 200, upper end cover 200 keeps away from the inside wall of one side of apron is equipped with installation internal thread 230, the lateral wall of both sides is equipped with corresponding installation external screw thread 110 about the column tube 100, and upper end cover 200 and lower end cover 300 pass through threaded connection's mode detachable to be fixed at the upper and lower both ends of column tube 100.

As shown in fig. 2 and 3, the dispersing screen 500 includes a dispersing screen main body 510 and a sealing ring 520, the dispersing screen main body 510 is in a hollow truncated cone shape, the dispersing screen main body 510 includes an upper screen 511, a lower screen 512 and a side wall 513 connecting the upper screen and the lower screen, the diameter of the upper screen 511 is larger than that of the lower screen 512, the side wall 513 is provided with dispersing through holes, the sealing ring 520 is sleeved on the periphery of the side wall, and the upper side of the sealing ring 520 is connected and fixed on the side wall 513.

Dispersion sieve 500 is made of silica gel or rubber material, can warp, conveniently takes out dispersion sieve from the upper end cover, as shown in fig. 2, under the mounted state, the below of utensil hole sieve 210 sets up dispersion sieve 500, utensil hole sieve 210 with centre gripping between the dispersion sieve 500 go up filter membrane 400, dispersion sieve main part 510 is followed the upper end of column tube 100 stretches into in the intraductal of column tube, sealing ring 520 cover is established on the lateral wall of column tube 100. The lower filter membrane 700 is arranged above the sieve plate with holes on the inner side wall of the lower end cover 300, and the filter membrane pressing ring 800 is arranged in the inner cavity of the lower end cover 300 and pressed on the lower filter membrane 700. The phenomenon that the upper filter membrane and the lower filter membrane deviate in the using process of the chromatographic column is avoided, the upper filter membrane and the lower filter membrane are fully utilized, the filtration is comprehensive, and the filler is prevented from being blocked or lost.

First connecting pipe 600 with second connecting pipe 900 structure is the same, first connecting pipe 600 is kept away from one side of column tube is fixed with sealed lid 610 through threaded connection, the side of going up of sealed lid 610 is equipped with pipeline channel 611, be equipped with sealed piece 612 in the sealed lid 610, the center of sealed piece 612 is equipped with the pipeline hole, makes things convenient for sample tube or drain pipe to fix in first connecting pipe or second connecting pipe, first connecting pipe 600 is equipped with the confession first recess 620 that sealed piece settled. The sealing block 612 is a circular truncated cone, and the pipeline hole penetrates through the upper surface and the lower surface of the circular truncated cone.

Filter membrane clamping ring 800 is made for rubber or silica gel, can warp, makes things convenient for filter membrane clamping ring 800 to take out in lower end cover 300, changes down the filter membrane, also conveniently washs the dead angle simultaneously. The second connection pipe 900 is provided with a cock valve 910, which is convenient to close when not in use, and prevents liquid in the chromatographic column from running off to cause dry column.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention; the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance, and furthermore, unless otherwise explicitly stated or limited, the terms "mounted," "connected," and "connected" are to be construed broadly and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art will understand that various modifications can be made to the embodiments described in the foregoing embodiments, or some or all of the technical features of the embodiments can be equivalently replaced, and the modifications or the replacements do not make the essence of the corresponding technical solutions depart from the scope of the embodiments of the present invention.

Claims

1. A novel purification column is used in production of liraglutide, includes hollow column tube, its characterized in that: the upper end cover and the lower end cover are detachably fixed at the upper end and the lower end of the column tube respectively, an upper filter membrane and a dispersion sieve are arranged in the upper end cover, the upper filter membrane is arranged above the dispersion sieve, a first connecting pipe is fixed above the upper end cover, a lower filter membrane and a filter membrane pressing ring are arranged in the lower end cover, and a second connecting pipe is fixed below the lower end cover.

2. A novel purification column for liraglutide production according to claim 1, wherein: the utility model discloses a novel upper end cover, including upper end cover, lower end cover, upper end cover, internal wall, first connecting pipe, upper end cover and lower end cover, the upper end cover with the lower end cover structure is the same, the inside wall of upper end cover is fixed with the hole sieve, the hole sieve will the upper end cover separate into about two-layer, the middle part of the apron of upper end cover is equipped with connect the via hole, be equipped with the internal thread on connect the via hole's the lateral wall, the downside of first connecting pipe is equipped with the corresponding external screw thread, first connecting pipe passes through threaded connection and fixes on the upper end cover, the upper end cover is kept away from the inside wall of one side of apron is equipped with the installation internal thread, the lateral wall of both sides is equipped with the installation external screw thread that corresponds about the pillar pipe.

3. A novel purification column for liraglutide production according to claim 2, wherein: the dispersion sieve is including dispersion sieve main part and sealing ring, the dispersion sieve main part is hollow round platform form, the dispersion sieve main part includes the upper screen, sifts down and connects the lateral wall of upper screen and sifting down, the diameter of upper screen is greater than the diameter of sifting down, be equipped with the dispersion through-hole on the lateral wall, the sealing ring cover is established the periphery of lateral wall, the upside of sealing ring is connected and is fixed on the lateral wall.

4. A novel purification column for liraglutide production according to claim 3, wherein: the dispersion sieve is that silica gel or rubber material make, under the installation, the below of utensil hole sieve plate sets up the dispersion sieve, utensil hole sieve with centre gripping between the dispersion sieve goes up the filter membrane, the dispersion sieve main part is followed the upper end of column tube stretches into in the pipe of column tube, the sealing ring cover is established on the lateral wall of column tube.

5. A novel purification column for liraglutide production according to claim 2, wherein: the lower filter membrane is arranged above the sieve plate with the holes on the inner side wall of the lower end cover, and the filter membrane pressing ring is arranged in the inner cavity of the lower end cover and pressed on the lower filter membrane.

6. A novel purification column for liraglutide production according to claim 1, wherein: the first connecting pipe and the second connecting pipe are identical in structure, a sealing cover is fixedly connected to one side, far away from the column pipe, of the first connecting pipe through threads, a pipeline channel is arranged on the upper side face of the sealing cover, a sealing block is arranged in the sealing cover, a pipeline hole is formed in the center of the sealing block, and a first groove for the sealing block to be arranged is formed in the first connecting pipe.

7. A novel purification column for liraglutide production according to claim 6, wherein: the sealing block is a circular truncated cone, and the pipeline hole penetrates through the upper surface and the lower surface of the circular truncated cone.

8. A novel purification column for liraglutide production according to claim 1, wherein: the filter membrane clamping ring is made of rubber or silica gel.

9. A novel purification column for liraglutide production according to claim 1, wherein: and a cock type valve is arranged on the second connecting pipe.