CN215923126U

CN215923126U - Kit for flow-type fluorescence detection method

Info

Publication number: CN215923126U
Application number: CN202121039112.XU
Authority: CN
Inventors: 熊霞; 陈扬
Original assignee: Jiangxi Saiji Biotechnology Co ltd
Current assignee: Jiangxi Saiji Biotechnology Co ltd
Priority date: 2021-05-17
Filing date: 2021-05-17
Publication date: 2022-03-01
Anticipated expiration: 2031-05-17

Abstract

The utility model discloses a kit for a flow-type fluorescence detection method, which comprises a box body, partition plates and a fixing plate, wherein the inner wall of the box body is fixedly connected with a plurality of partition plates, and the lower surfaces of the partition plates are fixedly connected with the fixing plate, and the kit is characterized in that: a test tube placing cavity and a liquid storage cavity are formed in the box body through a partition plate, a protective cover is movably connected to the top of the test tube placing cavity, a single-opening structure is arranged on the upper surface of the protective cover, and the protective cover is movably connected with the box body through a buckle structure; through the arrangement of the protective cover, the single-opening structure and the buckle structure, the test tube can be prevented from being broken due to collision, the protection performance is improved, meanwhile, the reagent can be extracted by single opening, the protective cover can be completely opened to extract a plurality of reagents, the complex operation of multiple single opening is avoided, and the efficiency of detecting and extracting the liquid is improved; through white light lamp and semiconductor cooler's setting, can provide arouse condition and lighting condition for fluorescence detection to refrigerate reagent, avoid light and heat to make reagent temperature produce the change and influence the observation effect.

Description

Kit for flow-type fluorescence detection method

Technical Field

The utility model relates to the field of kits, in particular to a kit for a flow-type fluorescence detection method.

Background

The flow-type fluorescence detection kit is generally used in hospitals, pharmaceutical enterprises, medical research institutions and third-party detection departments, can enable detection personnel to get rid of heavy reagent preparation and optimization processes, greatly improves detection efficiency, and comprises 6 types of capture microspheres with different fluorescence intensities in capture microsphere mixed liquid in the flow-type fluorescence detection kit, wherein the surfaces of the capture microspheres are respectively coated with interleukin 2, interleukin 4, interleukin 6, interleukin 10, tumor necrosis factor alpha and interferon gamma specific antibodies, the capture microspheres are respectively combined with the interleukin 2, interleukin 4, interleukin 6, interleukin 10, tumor necrosis factor alpha and interferon gamma specific antibodies in a sample to be detected and then combined with a PE-labeled fluorescence detection reagent, so as to capture microspheres, the sample to be detected and the detection antibodies to form a double-antibody sandwich complex (capture microspheres + sample to be detected + detection antibodies), and by analyzing the fluorescence intensity of the double-antibody sandwich complex, obtain the interleukin 2 of the sample that awaits measuring, interleukin 4, interleukin 6, interleukin 10, the content of tumor necrosis factor alpha and interferon gamma, the current kit that is used for STREAMING fluorescence detection method needs the multiple reagent bottle of splendid attire simultaneously when using, the broken condition of too many easy bumps of reagent bottle, the protectiveness is lower, get when putting reagent also need open different reagent bottles many times, complex operation, detect and get liquid inefficiency, and inconvenient collection liquid when carrying out STREAMING fluorescence detection, can not provide the illumination, the change of light intensity is lower and temperature all can influence the observation effect.

SUMMERY OF THE UTILITY MODEL

In view of the problems in the prior art, the utility model discloses a kit for a flow-type fluorescence detection method, which adopts the technical scheme that the kit comprises a kit body, a plurality of clapboards and a fixing plate, wherein the inner wall of the kit body is fixedly connected with the plurality of clapboards, the lower surfaces of the clapboards are fixedly connected with the fixing plate, a test tube placing cavity and a liquid storage cavity are formed in the kit body through the clapboards, a reagent in a test tube and a large amount of test liquid can be separately placed through the arrangement of the test tube placing cavity and the liquid storage cavity, the reagent and the large amount of test liquid are convenient to distinguish, a protective cover is movably connected to the top of the test tube placing cavity, a single-opening structure is arranged on the upper surface of the protective cover, the protective cover is movably connected with the kit body through a buckle structure, and the protective cover, the test tubes can be separated through the matching arrangement of the protective cover, the single-opening structure and the buckle structure, the test tube is prevented from being broken due to collision, simultaneously, the liquid in the test tube can be taken out only by opening the protective cover, the liquid in a plurality of test tubes can also be extracted by opening the protective cover once, the complex operation of single opening for a plurality of times is avoided, the efficiency of liquid taking in detection is improved, the top of the liquid storage cavity is provided with the stop cover, the upper surfaces of the protective cover and the stop cover are both provided with the note stickers, the names of all reagents can be conveniently marked through the arrangement of the note stickers, the extraction of the reagents is prevented from being wrong, the storage battery is arranged between the lower surface of the fixed plate and the inner bottom wall of the box body, the inner bottom walls of the test tube placing cavity and the liquid storage cavity are both fixedly connected with the white light lamp, the inner walls of the test tube placing cavity and the liquid storage cavity are both fixedly connected with the semiconductor refrigerator, the storage battery, the white light lamp and the semiconductor refrigerator are all electrically connected, and the storage battery, the white light lamp and the semiconductor refrigerator are arranged through the storage battery, the white light lamp and the semiconductor refrigerator, can utilize the battery to supply power for white light lamp and semiconductor cooler, the white light lamp provides the excitation condition for fluorescence detection, and the semiconductor cooler can refrigerate, and the light and heat of avoiding the white light lamp to produce makes reagent temperature produce the change and leads to the observation effect to reduce.

According to a preferred technical scheme, handles are fixedly connected to two sides of the box body, a push switch is arranged below each handle, a charging interface is arranged on one side of each push switch, the push switches and the charging interfaces are electrically connected with a storage battery, the handles can conveniently lift the whole kit to move through the arrangement of the handles, the push switches can control the white light lamp and the semiconductor refrigerator, and the charging interfaces can conveniently charge the storage battery, so that the cruising ability of the kit is improved.

As a preferable technical scheme, the protective cover is movably connected with the partition plate through a hinge, the upper surface of the blocking cover is fixedly connected with a handle, the hinge can prevent the protective cover from falling to cause reagent in the test tube to flow out through the arrangement of the hinge and the handle, and the handle can conveniently open the blocking cover.

According to the preferable technical scheme, the front side of the box body is provided with scale marks, the water faucet is arranged below the scale marks, the scale marks can be used for conveniently checking the liquid content in the liquid storage cavity from the outside through the arrangement of the scale marks and the water faucet, and the water faucet can be used for conveniently receiving and taking the liquid reagent.

According to a preferred technical scheme of the utility model, the single-opening structure comprises a rotary cover, a boss and a threaded hole, the boss is fixedly connected to the upper surface of the rotary cover, the rotary cover is in threaded connection with the threaded hole, the threaded hole is formed in the upper surface of the protective cover, and the boss can be used for screwing out the rotary cover through the arrangement of the rotary cover, the boss and the threaded hole, so that the single-opening function of the protective cover is realized, and a single reagent can be conveniently extracted.

According to the preferable technical scheme, the buckle structure comprises the clamping block and the lock catch, the clamping block is fixedly connected to the two sides of the upper surface of the protective cover, the lock catch is clamped at the bottom of the clamping block, the lock catch is fixedly connected with the side wall of the box body, the protective cover can be locked with the box body by the clamping of the clamping block and the lock catch through the arrangement of the clamping block and the lock catch, the protective cover is prevented from being opened mistakenly, and the protective performance of the reagent in the test tube is improved.

According to a preferable technical scheme of the utility model, the lower surface of the protective cover is fixedly connected with a rubber cushion, the inner wall of the liquid storage cavity is fixedly connected with a stop block, the rubber cushion can protect the top of the test tube through the arrangement of the rubber cushion and the stop block, the top of the test tube is prevented from being crushed, and the stop block can limit the stop cover.

The utility model has the beneficial effects that: according to the utility model, through the matched arrangement of the protective cover, the single-opening structure and the buckle structure, when various reagent test tubes are contained at the same time, the test tubes can be separated, so that the test tubes are prevented from being broken due to collision, the protection performance is improved, meanwhile, the liquid in the test tubes can be taken out in a single opening mode, the protective cover can be opened once to extract the liquid in a plurality of test tubes, the complicated operation of multiple single opening is avoided, and the liquid taking efficiency is improved; through battery, white light lamp and semiconductor cooler's setting, can utilize the battery to supply power for white light lamp and semiconductor cooler, white light lamp provides excitation condition and lighting condition for fluorescence detection, and semiconductor cooler can refrigerate, and the light and heat of avoiding white light lamp to produce makes reagent temperature produce the change and leads to the observation effect to reduce.

Drawings

FIG. 1 is a schematic perspective view of the present invention;

FIG. 2 is a schematic side view of the cross-sectional structure of the present invention;

fig. 3 is a schematic diagram of the explosion structure of the single-open structure of the present invention.

In the figure: 1-box body, 2-partition board, 3-fixing board, 4-test tube placing cavity, 5-liquid storage cavity, 6-protective cover, 7-single-opening structure, 701-spiral cover, 702-boss, 703-threaded hole, 8-buckle structure, 801-clamping block, 802-locking buckle, 9-blocking cover, 10-note sticker, 11-storage battery, 12-white light lamp, 13-semiconductor refrigerator, 14-handle, 15-push switch, 16-charging interface, 17-hinge, 18-handle, 19-scale line, 20-tap, 21-rubber soft pad and 22-stop block.

Detailed Description

Example 1

As shown in fig. 1 to 3, the utility model discloses a reagent kit for a flow-type fluorescence detection method, which adopts the technical scheme that the reagent kit comprises a kit body 1, a plurality of partition plates 2 and a fixing plate 3, wherein the inner wall of the kit body 1 is fixedly connected with the partition plates 2, the lower surfaces of the partition plates 2 are fixedly connected with the fixing plate 3, a test tube placing cavity 4 and a liquid storage cavity 5 are formed in the kit body 1 through the partition plates 2, a test tube reagent and a large-amount reagent can be separately stored in the test tube placing cavity 4 and the liquid storage cavity 5, so that the reagent can be conveniently distinguished and extracted, a protecting cover 6 is movably connected to the top of the test tube placing cavity 4, a single-opening structure 7 is arranged on the upper surface of the protecting cover 6, the protecting cover 6 is movably connected with the kit body 1 through a buckle structure 8, when the reagent in a single test tube needs to be extracted, the single-opening structure 7 can be opened for extraction, when a plurality of reagents in test tubes need to be extracted, can open buckle structure 8 and extract the reagent in a plurality of test tubes in proper order, the top of liquid storage chamber 5 is provided with fender cover 9, and fender cover 9 can shelter from the top of liquid storage chamber 5, and reagent drips and takes place the mixed pollution when avoiding extracting the reagent, the upper surface of protective cover 6 and fender cover 9 all is provided with memo subsides 10, writes the name of corresponding reagent on the upper surface of memo subsides 10, can directly discern corresponding reagent when extracting the reagent, be provided with battery 11 between the lower surface of fixed plate 3 and the interior diapire of box body 1, the test tube is placed chamber 4 and the interior diapire of liquid storage chamber 5 and is all fixedly connected with white light lamp 12, the test tube is placed the equal fixedly connected with semiconductor cooler 13 of chamber 4 and the inner wall of liquid storage chamber 5, all electric connection between battery 11, white light lamp 12 and the semiconductor cooler 13, when the kit work, the storage battery 11 provides power for the white light lamp 12 and the semiconductor refrigerator 13, the white light lamp 12 illuminates the reagent to create an excitation condition, and the semiconductor refrigerator 13 cools the reagent to keep the temperature constant, so that the observation effect of fluorescence detection is prevented from being influenced by illumination and temperature rise.

As a preferred technical scheme of the present invention, handles 14 are fixedly connected to both sides of the box body 1, a push switch 15 is arranged below the handles 14, a charging interface 16 is arranged at one side of the push switch 15, both the push switch 15 and the charging interface 16 are electrically connected to the storage battery 11, the charging interface 16 is connected to a power supply, the storage battery 11 is fully charged, and then the push switch 15 is turned on, so that the white light 12 and the semiconductor refrigerator 13 can start to work.

As a preferred technical scheme of the utility model, the protective cover 6 is movably connected with the partition plate 2 through a hinge 17, the upper surface of the blocking cover 9 is fixedly connected with a handle 18, the hinge 17 is movably connected with the protective cover 6 to prevent the protective cover 6 from being separated and falling, so that the stability of the test tube is ensured, and the handle 18 is convenient for opening the blocking cover 9 to add liquid reagent.

As a preferable technical solution of the present invention, the front surface of the box body 1 is provided with a scale mark 19, a water tap 20 is arranged below the scale mark 19, the water tap 20 is opened to allow the liquid reagent in the liquid storage chamber 5 to flow out and take, and the content of the liquid in the liquid storage chamber 5 can be known by observing the scale mark 19 from the outside, so that the liquid reagent can be conveniently added.

As a preferred technical solution of the present invention, the single-open structure 7 includes a screw cap 701, a boss 702 and a threaded hole 703, the boss 702 is fixedly connected to the upper surface of the screw cap 701, the screw cap 701 is in threaded connection with the threaded hole 703, the threaded hole 703 is disposed on the upper surface of the protective cover 6, and when the protective cover 6 needs to be opened singly, the boss 702 can be used to screw out the screw cap 701, so as to extract the reagent in a single test tube.

As a preferred technical solution of the present invention, the fastening structure 8 includes a fastening block 801 and a latch 802, both sides of the upper surface of the protective cover 6 are fixedly connected with the fastening block 801, the bottom of the fastening block 801 is fastened with the latch 802, and the latch 802 is fixedly connected with the side wall of the box body 1, so that when a plurality of reagents in test tubes need to be extracted, the fastening block 801 and the latch 802 can be disengaged, the protective cover 6 is completely opened, and the extraction of the plurality of reagents can be simultaneously achieved.

As a preferred technical solution of the present invention, a rubber cushion 21 is fixedly connected to a lower surface of the protective cover 6, a stopper 22 is fixedly connected to an inner wall of the liquid storage chamber 5, and when the protective cover 6 is closed, a lower surface of the rubber cushion 21 contacts with an upper surface of the test tube to protect and buffer the upper surface of the test tube, thereby preventing the test tube from being crushed and swaying.

The working principle of the utility model is as follows: firstly, the battery 11 is charged fully by the charging interface 16, then the push switch 15 is turned on, so that the white light lamp 12 and the semiconductor refrigerator 13 are both turned on, the battery 11 provides power for the white light lamp 12 and the semiconductor refrigerator 13, the white light lamp 12 illuminates the reagent to create an excitation condition, the semiconductor refrigerator 13 cools the reagent to keep the temperature constant, so as to avoid the illumination temperature rise from influencing the observation effect of the fluorescence detection, when the reagent in a single test tube needs to be extracted, the rotary cover 701 can be screwed out by the boss 702 to extract the reagent in the single test tube, when the reagents in a plurality of test tubes need to be extracted, the fixture block 801 and the lock catch 802 can be unlocked, the protective cover 6 is completely opened, the reagents in the plurality of test tubes are extracted at the same time, then the water tap 20 is opened to receive the reagent, then the liquid content in the liquid storage cavity 5 can be known by observing the scale mark 19 from the outside, liquid reagent is conveniently added, and finally, the push switch 15 is closed after the fluorescence detection is finished, so that the white light lamp 12 and the semiconductor refrigerator 13 stop working.

The circuit connections according to the utility model are conventional means used by the person skilled in the art and can be suggested by a limited number of tests, which are common knowledge.

Components not described in detail herein are prior art.

Although the present invention has been described in detail with reference to the specific embodiments, the present invention is not limited to the above embodiments, and various changes and modifications without inventive changes may be made within the knowledge of those skilled in the art without departing from the spirit of the present invention.

Claims

1. The kit for the flow-type fluorescence detection method comprises a kit body (1), partition plates (2) and a fixing plate (3), wherein the inner wall of the kit body (1) is fixedly connected with the partition plates (2), the lower surfaces of the partition plates (2) are fixedly connected with the fixing plate (3), and the kit is characterized in that: the test tube placing cavity (4) and the liquid storage cavity (5) are arranged in the box body (1) through a partition plate (2), the top of the test tube placing cavity (4) is movably connected with a protective cover (6), the upper surface of the protective cover (6) is provided with a single opening structure (7), the protective cover (6) is movably connected with the box body (1) through a buckle structure (8), the top of the liquid storage cavity (5) is provided with a blocking cover (9), the upper surfaces of the protective cover (6) and the blocking cover (9) are both provided with a note sticker (10), a storage battery (11) is arranged between the lower surface of the fixing plate (3) and the inner bottom wall of the box body (1), the inner bottom walls of the test tube placing cavity (4) and the liquid storage cavity (5) are both fixedly connected with a white light lamp (12), and the inner walls of the test tube placing cavity (4) and the liquid storage cavity (5) are both fixedly connected with a semiconductor refrigerator (13), the storage battery (11), the white light lamp (12) and the semiconductor refrigerator (13) are electrically connected.

2. The kit according to claim 1, wherein the kit comprises: the battery charging box is characterized in that handles (14) are fixedly connected to the two sides of the box body (1), a push switch (15) is arranged below the handles (14), a charging interface (16) is arranged on one side of the push switch (15), and the push switch (15) and the charging interface (16) are electrically connected with a storage battery (11).

3. The kit according to claim 1, wherein the kit comprises: protective cover (6) pass through hinge (17) and baffle (2) swing joint, the last fixed surface of flap (9) is connected with handle (18).

4. The kit according to claim 1, wherein the kit comprises: the front of the box body (1) is provided with scale marks (19), and a water tap (20) is arranged below the scale marks (19).

5. The kit according to claim 1, wherein the kit comprises: singly open structure (7) including spiral cover (701), boss (702) and screw hole (703), the last fixed surface of spiral cover (701) is connected with boss (702), spiral cover (701) and screw hole (703) threaded connection, screw hole (703) set up in the upper surface of protective cover (6).

6. The kit according to claim 1, wherein the kit comprises: buckle structure (8) include fixture block (801) and hasp (802), the equal fixedly connected with fixture block (801) in both sides of protective cover (6) upper surface, the bottom joint of fixture block (801) has hasp (802), hasp (802) and the lateral wall fixed connection of box body (1).

7. The kit according to claim 1, wherein the kit comprises: the lower surface fixedly connected with rubber cushion (21) of protective cover (6), the inner wall fixedly connected with stopper (22) of liquid storage chamber (5).