CN215906206U - Biological strain reinforced culture equipment - Google Patents

Abstract

The utility model discloses a biological strain intensified culture device which comprises a culture tank, an aeration cavity, a culture chamber, a nutrient tube and a stirring paddle, wherein the bottom of the culture tank is provided with the aeration cavity, the culture chamber is arranged in the culture tank above the aeration cavity, a stirring rod is arranged in the center of the interior of the culture chamber, the stirring paddles are fixed on the outer walls of two sides of the stirring rod, one side of the top end of the culture tank is fixed with a microorganism adding port, the outer wall of one side of the culture tank is provided with a detection box, the outer wall of one side of the culture tank, which is far away from the detection box, is provided with a return tube, the outer wall of the culture tank below the return tube is provided with a feeding tube, and the outer wall of the culture tank below the feeding tube is provided with an air guide tube. The utility model can continuously carry out microorganism multiplication culture, improves the culture efficiency, reduces the consumption of water resources, improves the sealing property of the culture environment, and ensures the qualification and the safety of the culture environment.

Description

Biological strain reinforced culture equipment

Technical Field

The utility model relates to the technical field of biological piles, in particular to a biological strain enhanced culture device.

Background

The bio-heap technology is to adopt artificial strengthening measures to the polluted soil heap to promote the growth of indigenous microorganisms or exogenous microorganisms having the capacity of degrading pollutants in the soil and degrade the pollutants in the soil. The bio-heap technology has good effect on easily biodegradable organic matters such as polluted soil and petroleum hydrocarbon in oil sludge, is not suitable for repairing the soil polluted by heavy metal and difficultly degradable organic pollutants, and has poor repairing effect on clay-polluted soil. The repair cost of the bio-heap technology is relatively low, related supporting facilities can be produced and manufactured in a set, the bio-heap technology is widely applied to repair of the soil which is easily biodegraded and polluted by petroleum hydrocarbon and the like abroad, and the technology is mature. The existing biological pile repairing technology mainly has the following problems aiming at the polluted soil repairing: firstly, the repair process is relatively long, and particularly, for some long-chain heterocyclic organic matters which are difficult to degrade, the time from half a year to one year is required, so that the popularization and the application of the bio-heap repair technology are greatly limited; secondly, a large amount of percolate generated in the process of restoring the biological heap also needs to be provided with a special wastewater treatment facility, so that the restoring cost is increased; finally, in the process of soil remediation by the biological pile, because the survival capability of microorganisms is poor, biological strains need to be continuously added, which greatly increases the operation cost of the biological pile, and therefore, a biological strain enhanced culture device is urgently needed to solve the problems.

The culture equipment on the market is various and can basically meet the use requirements of people, but certain defects still exist, the existing culture equipment cannot continuously perform microorganism multiplication culture, the culture efficiency of microorganisms is low, and the consumption of water resources is high.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a biological strain intensified culture device, which solves the problems that the culture device in the background technology cannot continuously perform microorganism multiplication culture, and has low efficiency and high cost.

In order to achieve the purpose, the utility model provides the following technical scheme: an intensified culture device for biological strains, which comprises a culture tank, an aeration cavity, a culture chamber, a nutrient canal and a stirring paddle, wherein the aeration cavity is arranged at the bottom of the culture tank, a culture chamber is arranged in the culture tank above the aeration cavity, a first filler layer is arranged in the culture chamber, a second filler layer is arranged in the culture chamber above the first filler layer, a stirring rod is arranged at the center position in the culture chamber and sequentially penetrates through the second filler layer and the first filler layer, and the outer walls of the two sides of the stirring rod are both fixed with stirring paddles, one side of the top end of the culture tank is fixed with a microorganism adding port, and the outer wall of one side of the culture tank is provided with a detection box, the outer wall of one side of the culture tank far away from the detection box is provided with a return pipe, and the outer wall of the culture tank below the return pipe is provided with an adding pipe, and the outer wall of the culture tank below the adding pipe is provided with an air duct.

Preferably, the central point department on culture tank top puts and is fixed with the aircraft bonnet, just the internally mounted of aircraft bonnet has the motor, and the output of motor passes through the shaft coupling and installs the pivot, the bottom of pivot extend to the inside of cultivateing the room and with the top fixed connection of puddler, be convenient for drive puddler make its rotation.

Preferably, the internally mounted of aeration chamber has the aeration coil pipe, and the aeration coil pipe is linked together with the air duct, just the inside of aeration coil pipe all is fixed with the connecting pipe, and equidistant aeration pipe is all installed on the top of aeration coil pipe, and the top of aeration pipe extends to the inside of cultivateing the room, is convenient for carry out the aeration to the cultivation room.

Preferably, the manometer is installed to the bottom of detection case, and the sense terminal of manometer extends to the inside of cultivateing the room, is convenient for detect the inside pressure of culture tank.

Preferably, the internally mounted of detection case has temperature sensor, just the detection case internally mounted of temperature sensor top has humidity transducer, and humidity transducer and temperature sensor's sense terminal all extends to the inside of cultivateing the room, is convenient for detect the inside humiture of culture tank.

Preferably, a positioning ring is fixed on the outer wall of the microorganism adding port, and positioning holes with equal intervals are formed in the positioning ring, so that the microorganism adding port is conveniently sealed.

Preferably, the top end of the microorganism adding port is provided with a sealing cover, and the bottom end of the sealing cover is provided with a sealing ring, so that the compression screw rod can be conveniently positioned.

Preferably, a water pipe is fixed at the top end of the culture tank on one side of the hood, and a nutrient pipe is installed at the top end of the culture tank on one side of the water pipe, so that water and nutrient substances required by microbial proliferation can be continuously supplemented to a culture chamber in the culture tank.

Preferably, all be fixed with equidistant ear piece on the outer wall of sealed lid, just the inside of ear piece is provided with the screw hole, and the inside of screw hole is provided with sticiss the screw rod, sticiss the inside that the bottom of screw rod extends to the locating hole for the sealing washer is extruded, has further improved the leakproofness between microorganism interpolation mouth and the sealed lid.

Compared with the prior art, the utility model has the beneficial effects that: the enhanced culture equipment for the biological strains can continuously perform microorganism multiplication culture, improves the culture efficiency, reduces the consumption of water resources, improves the sealing property of the culture environment, and ensures the qualification and the safety of the culture environment;

(1) by arranging the aeration coil pipe, the connecting pipe, the aeration pipe, the stirring rod, the stirring paddle, the motor, the rotating shaft, the water pipe, the nutrition pipe and the return pipe, water and nutrient substances required by microorganism proliferation are continuously supplemented into the culture chamber through the water pipe and the nutrition pipe respectively, exogenous microorganisms or indigenous microorganisms required to be proliferated are added into the culture chamber through the microorganism adding port, the motor drives the stirring rod to rotate through the rotating shaft, the stirring rod drives the stirring paddle to rotate, the microorganism is stirred, external gas enters the aeration coil pipe through the gas guide pipe and is input into the culture chamber through the aeration pipe to be aerated, and after continuous stirring and aeration culture, the microorganism in the culture chamber is proliferated in large quantity, the microorganism can be continuously proliferated, so that microorganism proliferation culture can be continuously carried out, the return pipe is arranged so that the biological heap leachate flows back to the interior of the culture tank, the leachate can be treated to a certain extent, the wastewater treatment cost is reduced, the consumption of external water in a biological heap water supply system can be reduced, water is saved, indigenous microorganisms in the leachate are effectively proliferated, the treatment effect of a biological heap is obviously improved, and the treatment time is shortened;

(2) the sealing cover is covered at the top end of the microorganism adding port by the positioning ring, the positioning hole, the sealing cover, the sealing ring, the lug block, the threaded hole and the pressing screw rod, the microorganism adding port is sealed, the pressing screw rod is rotated to be screwed into the positioning hole, and the sealing ring at the bottom end of the sealing cover is extruded after the pressing screw rod is screwed, so that the sealing property between the microorganism adding port and the sealing cover is further improved, and the sealing property of a culture environment is ensured;

(3) through being provided with detection case, manometer, temperature sensor and humidity transducer, the manometer of detection case inside implements the detection to the inside pressure of culture tank, and the inside temperature sensor of detection case and humidity transducer implement the detection to the inside temperature and the humidity of culture tank respectively to the qualification and the security of cultivateing the environment have been guaranteed.

Drawings

FIG. 1 is a schematic front sectional view of the present invention;

FIG. 2 is a schematic view of an enlarged top view of the aeration coil according to the present invention;

FIG. 3 is a schematic view of a cross-sectional enlarged structure of a microorganism addition port according to the present invention;

FIG. 4 is a front view of the detection box according to the present invention;

FIG. 5 is a schematic diagram of an enlarged top view of the inspection box of the present invention.

In the figure: 1. a culture tank; 2. an aeration cavity; 3. an aeration coil pipe; 4. a culture chamber; 5. a first filler layer; 6. a second packing layer; 7. a detection box; 8. a microorganism addition port; 9. a stirring rod; 10. a hood; 11. a motor; 12. a rotating shaft; 13. a water pipe; 14. a feeding tube; 15. a stirring paddle; 16. a return pipe; 17. a feeding pipe; 18. an air duct; 19. a connecting pipe; 20. an aeration pipe; 21. positioning rings; 22. positioning holes; 23. a sealing cover; 24. a seal ring; 25. an ear piece; 26. a threaded hole; 27. pressing the screw; 28. a pressure gauge; 29. a temperature sensor; 30. a humidity sensor.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and all other embodiments obtained by a person of ordinary skill in the art based on the embodiments of the present invention without any creative efforts belong to the protection scope of the present invention.

Referring to fig. 1-5, an embodiment of the present invention is shown: a biological strain intensive culture device comprises a culture tank 1, an aeration cavity 2, a culture chamber 4, a nutrient tube 14 and a stirring paddle 15, wherein the bottom of the culture tank 1 is provided with the aeration cavity 2, the culture chamber 4 is arranged in the culture tank 1 above the aeration cavity 2, and the aeration cavity 2 and the culture chamber 4 are both arranged in a hollowed mode;

a first filler layer 5 is arranged inside the culture chamber 4, and a second filler layer 6 is arranged inside the culture chamber 4 above the first filler layer 5;

a hood 10 is fixed at the center of the top end of the culture tank 1, the hood 10 is welded with the culture tank 1, a motor 11 is installed inside the hood 10, a rotating shaft 12 is installed at the output end of the motor 11 through a coupler, the bottom end of the rotating shaft 12 extends into the culture chamber 4 and is fixedly connected with the top end of the stirring rod 9, and the stirring rod 9 is conveniently driven to rotate;

a water pipe 13 is fixed at the top end of the culture tank 1 at one side of the hood 10, a nutrient pipe 14 is installed at the top end of the culture tank 1 at one side of the water pipe 13, and the water pipe 13 and the nutrient pipe 14 are both communicated with the culture chamber 4, so that moisture and nutrient substances required by microorganism proliferation can be continuously supplemented into the culture chamber 4 in the culture tank 1;

the aeration coil 3 is arranged in the aeration cavity 2, the aeration coil 3 is arranged in the aeration cavity 2 through bolts, the aeration coil 3 is communicated with the air guide pipe 18, the connecting pipes 19 are fixed in the aeration coil 3, the top ends of the aeration coil 3 are provided with the aeration pipes 20 at equal intervals, and the top ends of the aeration pipes 20 extend to the inside of the culture chamber 4, so that the culture chamber 4 is aerated;

a stirring rod 9 is arranged at the central position inside the culture chamber 4, the stirring rod 9 is rotatably connected with the culture tank 1, the stirring rod 9 sequentially penetrates through the second packing layer 6 and the first packing layer 5, stirring paddles 15 are fixed on the outer walls of two sides of the stirring rod 9, a microorganism adding port 8 is fixed on one side of the top end of the culture tank 1, a detection box 7 is installed on the outer wall of one side of the culture tank 1, and the detection box 7 is installed on the side wall of the culture tank 1 through bolts;

the bottom of the detection box 7 is provided with a pressure gauge 28, and the detection end of the pressure gauge 28 extends into the culture chamber 4, so that the pressure in the culture tank 1 can be conveniently detected;

a temperature sensor 29 is arranged in the detection box 7, a humidity sensor 30 is arranged in the detection box 7 above the temperature sensor 29, and the detection ends of the humidity sensor 30 and the temperature sensor 29 extend into the culture chamber 4, so that the temperature and the humidity in the culture tank 1 can be conveniently detected;

a positioning ring 21 is fixed on the outer wall of the microorganism adding port 8, and positioning holes 22 with equal intervals are arranged inside the positioning ring 21, so that the microorganism adding port 8 is conveniently sealed;

the top end of the microorganism adding port 8 is provided with a sealing cover 23, the bottom end of the sealing cover 23 is provided with a sealing ring 24, the sealing ring 24 is adhered to the sealing cover 23, and the bottom end of the sealing ring 24 is tightly attached to the top end of the microorganism adding port 8, so that the compression screw 27 can be conveniently positioned;

the outer walls of the sealing covers 23 are all fixed with equally spaced lug blocks 25, the lug blocks 25 are welded with the sealing covers 23, threaded holes 26 are formed in the lug blocks 25, pressing screws 27 are arranged in the threaded holes 26, and the bottom ends of the pressing screws 27 extend into the positioning holes 22, so that the sealing rings 24 are extruded, and the sealing performance between the microorganism adding openings 8 and the sealing covers 23 is further improved;

a return pipe 16 is arranged on the outer wall of one side of the culture tank 1, which is far away from the detection box 7, a feeding pipe 17 is arranged on the outer wall of the culture tank 1 below the return pipe 16, the return pipe 16 and the feeding pipe 17 are both communicated with the culture chamber 4, and an air guide pipe 18 is arranged on the outer wall of the culture tank 1 below the feeding pipe 17.

When the embodiment of the application is used: first, water and nutrients necessary for growth of microorganisms are continuously supplied to the culture chamber 4 inside the culture tank 1 through the water pipe 13 and the nutrient pipe 14, and exogenous microorganisms or indigenous microorganisms to be grown are introduced into the culture chamber 4 through the microorganism addition port 8. Secondly, cover sealed lid 23 on the top of microorganism interpolation mouth 8, seal microorganism interpolation mouth 8, rotate again and sticiss screw rod 27 and screw it into the inside of locating hole 22, sticis screw rod 27 and screw the back, and the sealing washer 24 of sealed lid 23 bottom is extruded, has further improved the leakproofness between microorganism interpolation mouth 8 and sealed lid 23 to the leakproofness of culture environment has been guaranteed. Then, the motor 11 is controlled to rotate the stirring rod 9 through the rotating shaft 12, the stirring rod 9 drives the stirring paddle 15 to rotate, the microorganisms are stirred, meanwhile, the outside air enters the inside of the aeration coil 3 through the air duct 18, the air is filled in the inside of the aeration coil 3 through the connecting pipe 19, and is input into the inside of the culture chamber 4 through the aeration pipe 20 for aeration. Through continuous stirring and aeration culture, the microorganisms inside the culture chamber 4 are proliferated in a large amount, and the microorganisms can still be continuously proliferated in the process of transferring out the cultured culture. Therefore, the culture equipment can continuously carry out microorganism multiplication culture, and cultures in the culture chamber 4 are added into the biological heap through the adding pipe 17, so that amplified strains are provided for the biological heap, the treatment effect of the biological heap is obviously improved, and the treatment time is shortened. The arrangement of the return pipe 16 can achieve the following effects: firstly, leachate can be recovered so that the biological heap leachate flows back to the interior of the culture tank 1, the leachate can be treated to a certain extent, and the wastewater treatment cost is reduced; and finally, leachate from the biological heap can be used for carrying out indigenous microorganism species and quantity to a certain extent, and the effect of carrying out indigenous microorganism proliferation for the biological heap remediation is achieved. In the process of cultivation, the pressure gauge 28 inside the detection box 7 is used for detecting the pressure inside the cultivation tank 1, the temperature sensor 29 and the humidity sensor 30 inside the detection box 7 are used for detecting the temperature and the humidity inside the cultivation tank 1 respectively, so that the qualification and the safety of the cultivation environment are guaranteed, and the air release valve is installed at the top end of the cultivation tank 1, so that the air pressure of the whole device can be controlled, and the work of the biological strain strengthening cultivation equipment is completed.

Claims (9)

1. The biological strain intensive culture equipment is characterized by comprising a culture tank (1), an aeration cavity (2), a culture chamber (4), a nutrient tube (14) and a stirring paddle (15), wherein the bottom of the culture tank (1) is provided with the aeration cavity (2), the culture chamber (4) is arranged inside the culture tank (1) above the aeration cavity (2), a first packing layer (5) is arranged inside the culture chamber (4), a second packing layer (6) is arranged inside the culture chamber (4) above the first packing layer (5), the stirring rod (9) is arranged at the central position inside the culture chamber (4), the stirring rod (9) sequentially penetrates through the second packing layer (6) and the first packing layer (5), the stirring paddles (15) are fixed on the outer walls of two sides of the stirring rod (9), a microorganism adding port (8) is fixed on one side of the top end of the culture tank (1), and install on the outer wall of culture tank (1) one side detection case (7), install on the outer wall of culture tank (1) one side of keeping away from detection case (7) back flow pipe (16), just install on the culture tank (1) outer wall of back flow pipe (16) below and throw feeder tube (17), and install air duct (18) on the culture tank (1) outer wall of throwing feeder tube (17) below.

2. The apparatus for enhanced cultivation of biological species as claimed in claim 1, wherein: cultivate the central point department on jar (1) top and be fixed with aircraft bonnet (10), just the internally mounted of aircraft bonnet (10) has motor (11), and pivot (12) are installed through the shaft coupling to the output of motor (11), and the bottom of pivot (12) extends to the inside of cultivateing room (4) and with the top fixed connection of puddler (9).

3. The apparatus for enhanced cultivation of biological species as claimed in claim 1, wherein: the internally mounted of aeration chamber (2) has aeration coil pipe (3), and aeration coil pipe (3) are linked together with air duct (18), and the inside of aeration coil pipe (3) all is fixed with connecting pipe (19) to the top of aeration coil pipe (3) all installs equidistant aeration pipe (20), and the top of aeration pipe (20) extends to the inside of cultivateing room (4).

4. The apparatus for enhanced cultivation of biological species as claimed in claim 1, wherein: the bottom of the detection box (7) is provided with a pressure gauge (28), and the detection end of the pressure gauge (28) extends to the inside of the culture chamber (4).

5. The apparatus for enhanced cultivation of biological species as claimed in claim 1, wherein: the internally mounted of detection case (7) has temperature sensor (29), just detection case (7) internally mounted of temperature sensor (29) top has humidity transducer (30), and the sense terminal of humidity transducer (30) and temperature sensor (29) all extends to the inside of cultivateing room (4).

6. The apparatus for enhanced cultivation of biological species as claimed in claim 1, wherein: and a positioning ring (21) is fixed on the outer wall of the microorganism adding port (8), and positioning holes (22) with equal intervals are arranged inside the positioning ring (21).

7. The apparatus for enhanced cultivation of biological species as claimed in claim 1, wherein: the top end of the microorganism adding port (8) is provided with a sealing cover (23), and the bottom end of the sealing cover (23) is provided with a sealing ring (24).

8. The apparatus for enhanced cultivation of biological species as claimed in claim 2, wherein: a water pipe (13) is fixed at the top end of the culture tank (1) on one side of the hood (10), and a nutrient tube (14) is installed at the top end of the culture tank (1) on one side of the water pipe (13).

9. An enhanced biological species culture apparatus as claimed in claim 7 wherein: the outer wall of the sealing cover (23) is fixed with equidistant lug blocks (25), threaded holes (26) are formed in the lug blocks (25), pressing screws (27) are arranged in the threaded holes (26), and the bottom ends of the pressing screws (27) extend to the inner portions of the positioning holes (22).

Images