CN215843076U

CN215843076U - Monoclonal antibody preparation carrier constructs with ice bath device

Info

Publication number: CN215843076U
Application number: CN202122247281.9U
Authority: CN
Inventors: 国今; 李桂梅; 单虎
Original assignee: Qingdao Agricultural University
Current assignee: Qingdao Agricultural University
Priority date: 2021-09-16
Filing date: 2021-09-16
Publication date: 2022-02-18
Anticipated expiration: 2031-09-16

Abstract

The utility model provides an ice bath device for constructing a monoclonal antibody preparation vector, and relates to the technical field of ice bath devices. This monoclonal antibody preparation carrier founds uses ice bath device, including the heat preservation box, heat preservation box one side is rotated and is connected with the upper cover plate, the fixed frame of heat preservation box inner wall fixedly connected with, the inside board of placing that is equipped with of heat preservation box, place board bottom fixedly connected with rack, rack and fixed frame sliding connection, place the board top and seted up a plurality of draw-in grooves, place the board top and be equipped with the centre gripping subassembly with a plurality of draw-in groove one-to-one, this monoclonal antibody preparation carrier founds uses ice bath device, promotes splint through the spring that places the board draw-in groove outside, makes splint carry out the centre gripping to the test tube, guarantees that the test tube carries out the stability of ice bath in the heat preservation box inside, carries out the disinfection of disinfecting through the inside ultraviolet lamp of upper cover plate to the test tube.

Description

Monoclonal antibody preparation carrier constructs with ice bath device

Technical Field

The utility model relates to a monoclonal antibody preparation equipment technology, in particular to an ice bath device for constructing a monoclonal antibody preparation carrier, and belongs to the technical field of ice bath devices.

Background

The monoclonal antibody is an antibody which is highly uniform and only aims at a certain specific epitope and is generated by single B cell clone, and is usually prepared by adopting a hybridoma technology, a vector is subjected to one-step transformation experiment during preparation of the monoclonal antibody, ice bath is immediately carried out after heat shock is required in the transformation experiment, an ice bath device is used at the moment, the existing ice bath device is a box, a plurality of pieces of crushed ice are placed in the box, and after a centrifugal tube is inserted into a floating pad for heat shock, the centrifugal tube is directly inserted into the crushed ice for ice bath.

After carrying out the ice bath to the test tube, the test tube outside is too cold when operating personnel takes the test tube, can't take out the test tube, and the test tube can't carry out the ice bath in the inside of box to a batch of reagent at the back and handle simultaneously, male in-process when the test tube carries out the ice bath, and the centrifuging tube probably takes place the slope for liquid in the centrifuging tube can not fully carry out the ice bath, probably leads to the conversion experiment failure or the conversion rate is low.

SUMMERY OF THE UTILITY MODEL

Technical problem to be solved

The utility model aims to provide an ice bath device for constructing a monoclonal antibody preparation carrier, which aims to solve the problems that in the prior art, after a test tube is subjected to ice bath, when an operator takes the test tube, the outside of the test tube is too cold, the test tube cannot be taken out, meanwhile, the test tube cannot perform ice bath treatment on a next batch of reagents in a box body, and in the process of inserting the test tube in the ice bath, a centrifugal tube may incline, so that liquid in the centrifugal tube cannot be subjected to ice bath fully, and the transformation experiment may fail or the transformation rate is low.

(II) technical scheme

In order to achieve the purpose, the utility model is realized by the following technical scheme: monoclonal antibody preparation carrier founds uses ice bath device, including the heat preservation box, heat preservation box one side is rotated and is connected with the upper cover plate, the fixed frame of heat preservation box inner wall fixedly connected with, the inside board of placing that is equipped with of heat preservation box, place board bottom fixedly connected with rack, rack and fixed frame sliding connection, place the board top and seted up a plurality of draw-in grooves, it is equipped with the centre gripping subassembly with a plurality of draw-in groove one-to-one to place the board top, it is equipped with and carries the lifting component to place the board top, heat preservation box one side fixedly connected with deposits the case, deposit the inside cooling subassembly of case.

Preferably, carry and draw the subassembly and include the rotor plate, the rotor plate rotates to be connected in placing board top both sides, two the rotor plate outside all is equipped with the cover frame and with cover frame sliding connection, the cover frame outside rotates and is connected with the extension board.

Preferably, carry and draw the subassembly still includes the connecting rod, extension board one end with place the board and rotate and be connected, the connecting rod both ends respectively with two extension board fixed connection, through rotating the connecting rod, make the cover frame slide in the rotor plate outside to make extension board and rotor plate form the support, the pulling connecting rod will place the board and wholly take out fixed frame.

Preferably, the centre gripping subassembly includes two fixed plates, two the fixed plate sets up respectively in draw-in groove top both sides, the fixed plate with place board fixed connection, two the equal fixedly connected with spring in the relative one side of fixed plate, spring one end fixedly connected with splint, the inside bottom fixedly connected with of rack a plurality ofly with the installation section of thick bamboo of draw-in groove one-to-one, it is a plurality of installation section of thick bamboo inner wall fixedly connected with foam-rubber cushion promotes splint through the spring, makes two splint carry out a centre gripping to the test tube, guarantees that the test tube avoids squinting when carrying out the ice bath inside the box that keeps warm.

Preferably, the rack bottom is equipped with the filter, filter and heat preservation box inner wall fixed connection, the equal fixedly connected with refrigeration board in heat preservation box inner wall both sides carries out ice making to the inside water of heat preservation box through the refrigeration board and handles.

Preferably, deposit incasement wall fixedly connected with baffle, deposit incasement portion bottom and install the water pump, heat preservation box inner wall fixedly connected with shower nozzle, fixedly connected with connecting pipe between water pump and the shower nozzle, deposit the fixed pipe that fixedly connected with is used for the connection between case and the heat preservation box, will keep warm the box and deposit and carry out a circulation between the case through fixed pipe, the using water wisely has avoided the pollution.

Preferably, the cooling assembly comprises a refrigerator, the refrigerator is arranged at the top of the partition plate inside the storage box, the output end of the refrigerator is communicated with the heat preservation box, a temperature sensor is arranged inside the heat preservation box, the temperature inside the heat preservation box can be monitored by the temperature sensor, and the heat preservation box is refrigerated by the refrigerator.

Preferably, a plurality of ultraviolet lamps are installed inside the upper cover plate, a storage battery is installed inside the upper cover plate, the storage battery is electrically connected with the input end of the ultraviolet lamps, and the ultraviolet lamps are used for sterilizing the test tubes.

The utility model provides an ice bath device for constructing a monoclonal antibody preparation vector, which has the following beneficial effects:

1. this monoclonal antibody preparation carrier constructs uses ice bath device, promotes splint through the spring of placing the board draw-in groove outside, makes splint carry out the centre gripping to the test tube, guarantees that the test tube carries out the stability of ice bath in the box that keeps warm inside, carries out the disinfection of disinfecting through the inside ultraviolet lamp of upper cover plate to the test tube.

2. This monoclonal antibody preparation carrier founds uses ice bath device, and the cover frame through rotor plate outside sliding connection drives the extension board and rotates, makes extension board, rotor plate and places and form the support between the board top, and the pulling connecting rod will be placed the board and place the frame and wholly take out in the heat preservation box, has avoided the test tube too low temperature behind the ice bath, leads to the problem that can't take.

Drawings

FIG. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a front cross-sectional view of the present invention;

FIG. 3 is a schematic view of the placement board of the present invention;

FIG. 4 is a schematic diagram of a pull assembly of the present invention.

In the figure: 1. a heat preservation box; 2. an upper cover plate; 3. placing the plate; 4. a fixing frame; 5. placing a rack; 6. a rotating plate; 7. a support plate; 8. sleeving a frame; 9. a connecting rod; 10. a card slot; 11. a fixing plate; 12. a spring; 13. mounting the cylinder; 14. a storage box; 15. a water pump; 16. a refrigerator; 17. a spray head; 18. a filter plate; 19. a fixed tube; 20. a refrigeration plate; 21. an ultraviolet lamp; 22. and (4) clamping the plate.

Detailed Description

The embodiment of the utility model provides an ice bath device for constructing a monoclonal antibody preparation vector.

Referring to fig. 1, 2 and 3, the heat preservation box comprises a heat preservation box 1, one side of the heat preservation box 1 is rotatably connected with an upper cover plate 2, the inner wall of the heat preservation box 1 is fixedly connected with a fixed frame 4, a placing plate 3 is arranged in the heat preservation box 1, the bottom of the placing plate 3 is fixedly connected with a placing rack 5, the placing rack 5 is slidably connected with the fixed frame 4, the top of the placing plate 3 is provided with a plurality of clamping grooves 10, the top of the placing plate 3 is provided with clamping components which are in one-to-one correspondence with the clamping grooves 10, each clamping component comprises two fixing plates 11, the two fixing plates 11 are respectively arranged on two sides of the top of the clamping groove 10, the fixing plates 11 are fixedly connected with the placing plate 3, one sides of the two fixing plates 11 which are opposite to each other are fixedly connected with a spring 12, one end of each spring 12 is fixedly connected with a clamping plate 22, the bottom end in the placing rack 5 is fixedly connected with a plurality of mounting cylinders 13 which are in one-to the clamping grooves 10, and the inner walls of the mounting cylinders 13 are fixedly connected with sponge cushions, promote splint 22 through spring 12, make two splint 22 carry out a centre gripping to the test tube, guarantee that the test tube avoids the skew when carrying out the ice bath in heat preservation box 1 inside, place 3 tops of board and be equipped with and carry the pull-up subassembly, heat preservation box 1 one side fixedly connected with deposits case 14, deposits the inside cooling assembly of case 14.

It is specific, inside inserting the test tube and placing draw-in groove 10 that the board 3 tops were seted up to inside placing the test tube bottom in an installation section of thick bamboo 13, promoting splint 22 through spring 12 in the draw-in groove 10 outside, making splint 22 carry out the centre gripping to the test tube, guarantee that the test tube carries out the stability of ice bath in heat preservation box 1 inside, carry out sterilization through 2 inside ultraviolet lamps 21 of upper cover plate to the test tube.

Please refer to fig. 1, fig. 3, and fig. 4 again, the pulling assembly includes a rotating plate 6, the rotating plate 6 is rotatably connected to two sides of the top of the placing plate 3, two rotating plates 6 are both sleeved with a sleeve frame 8 and slidably connected with the sleeve frame 8, the sleeve frame 8 is rotatably connected with a support plate 7, the pulling assembly further includes a connecting rod 9, one end of the support plate 7 is rotatably connected with the placing plate 3, two ends of the connecting rod 9 are respectively fixedly connected with the two support plates 7, by rotating the connecting rod 9, the sleeve frame 8 slides outside the rotating plate 6, so that the support plate 7 and the rotating plate 6 form a support, and the connecting rod 9 is pulled to integrally take out the placing plate 3 and the fixing frame 4.

Specifically, after the test tube passes through the ice bath, through pulling connecting rod 9, make rotor plate 6 lift, the cover frame 8 through 6 outside sliding connection of rotor plate drives extension board 7 and rotates this moment, makes extension board 7, rotor plate 6 and places and form the support between the 3 tops of board, and pulling connecting rod 9 will place board 3 and rack 5 and wholly take out in heat preservation box 1, has avoided the test tube too low temperature behind the ice bath, leads to the problem that can't take.

Referring to fig. 1, 2 and 3 again, the bottom of the placing frame 5 is provided with a filter plate 18, the filter plate 18 is fixedly connected with the inner wall of the heat preservation box 1, both sides of the inner wall of the heat preservation box 1 are fixedly connected with refrigeration plates 20, the water in the heat preservation box 1 is subjected to ice making treatment through the refrigeration plates 20, the inner wall of the storage box 14 is fixedly connected with a partition plate, the bottom end of the interior of the storage box 14 is provided with a water pump 15, the model of the water pump 15 can be KFS, no specific requirement is made on the model of the water pump 15, the inner wall of the heat preservation box 1 is fixedly connected with a spray head 17, a connecting pipe is fixedly connected between the water pump 15 and the spray head 17, a fixing pipe 19 for connection is fixedly connected between the storage box 14 and the heat preservation box 1, a circulation is performed between the heat preservation box 1 and the storage box 14 through the fixing pipe 19, water is saved, pollution is avoided, the cooling assembly comprises a refrigerator 16, the refrigerator 16 can adopt the model of OT12701, do not do the injecing to the model of refrigerator 16, refrigerator 16 sets up in the 14 inside baffle tops of case, 16 output ends of refrigerator communicate with each other with heat preservation box 1, 1 internally mounted of heat preservation box has temperature sensor, be favorable to monitoring the temperature of 1 inside of heat preservation box through temperature sensor, and carry out refrigeration processing to heat preservation box 1 through

refrigerator

16, 2 internally mounted of upper cover plate have a plurality of

ultraviolet lamps

21, 2 internally mounted batteries of upper cover plate, battery and ultraviolet lamp 21 input electric connection, carry out germicidal treatment to the test tube through ultraviolet lamp 21.

Specifically, through starting water pump 15 when carrying out the ice bath to the test tube, make water pump 15 spray through shower nozzle 17 with inside water through the connecting pipe to carry out refrigeration treatment through refrigerator 16 to heat preservation box 1 is inside, come to monitor inside temperature through temperature sensor, and through heat preservation box 1 and the fixed pipe 19 of depositing the intercommunication between the case 14, make inside water can carry out a circulation and use.

The foregoing shows and describes the general principles and broad features of the present invention and advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the utility model as claimed. The scope of the utility model is defined by the appended claims and equivalents thereof.

Claims

1. Monoclonal antibody preparation carrier constructs uses ice bath device, including heat preservation box (1), its characterized in that: the utility model discloses a heat preservation box, including heat preservation box (1), board (3) are placed to inside being equipped with of heat preservation box (1), place board (3) bottom fixedly connected with rack (5), rack (5) and fixed frame (4) sliding connection, place board (3) top and seted up a plurality of draw-in grooves (10), place board (3) top and be equipped with the centre gripping subassembly with a plurality of draw-in grooves (10) one-to-one, it is equipped with at board (3) top and carries and draws the subassembly to place, heat preservation box (1) one side fixedly connected with deposits case (14), deposit the inside cooling subassembly of case (14).

2. The monoclonal antibody production vector construction ice bath device according to claim 1, characterized in that: carry and draw the subassembly to include rotor plate (6), rotor plate (6) rotate to be connected in placing board (3) top both sides, two rotor plate (6) outside all is equipped with cover frame (8) and with cover frame (8) sliding connection, cover frame (8) outside is rotated and is connected with extension board (7).

3. The monoclonal antibody production vector construction ice bath device according to claim 2, characterized in that: the lifting assembly further comprises a connecting rod (9), one end of each support plate (7) is rotatably connected with the placing plate (3), and two ends of the connecting rod (9) are fixedly connected with the two support plates (7) respectively.

4. The monoclonal antibody production vector construction ice bath device according to claim 1, characterized in that: the centre gripping subassembly includes two fixed plates (11), two fixed plate (11) set up respectively in draw-in groove (10) top both sides, fixed plate (11) with place board (3) fixed connection, two the equal fixedly connected with spring (12) in the relative one side of fixed plate (11), spring (12) one end fixedly connected with splint (22), a plurality of installation section of thick bamboo (13) with draw-in groove (10) one-to-one of rack (5) inside bottom fixedly connected with, it is a plurality of installation section of thick bamboo (13) inner wall fixedly connected with foam-rubber cushion.

5. The monoclonal antibody production vector construction ice bath device according to claim 1, characterized in that: the rack (5) bottom is equipped with filter (18), filter (18) and heat preservation box (1) inner wall fixed connection, the equal fixedly connected with refrigeration board (20) in heat preservation box (1) inner wall both sides.

6. The monoclonal antibody production vector construction ice bath device according to claim 1, characterized in that: deposit case (14) inner wall fixedly connected with baffle, deposit case (14) inside bottom and install water pump (15), heat preservation box (1) inner wall fixedly connected with shower nozzle (17), fixedly connected with connecting pipe between water pump (15) and shower nozzle (17), deposit fixed pipe (19) that fixedly connected with is used for the link between case (14) and heat preservation box (1).

7. The monoclonal antibody production vector construction ice bath device according to claim 5, characterized in that: the cooling assembly comprises a refrigerator (16), the refrigerator (16) is arranged at the top of the internal partition plate of the storage box (14), the output end of the refrigerator (16) is communicated with the heat preservation box (1), and a temperature sensor is arranged inside the heat preservation box (1).

8. The monoclonal antibody production vector construction ice bath device according to claim 1, characterized in that: a plurality of ultraviolet lamps (21) are installed inside the upper cover plate (2), a storage battery is installed inside the upper cover plate (2), and the storage battery is electrically connected with the input ends of the ultraviolet lamps (21).