CN215841734U - Liquid chromatography column - Google Patents
Liquid chromatography column Download PDFInfo
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- CN215841734U CN215841734U CN202023164765.9U CN202023164765U CN215841734U CN 215841734 U CN215841734 U CN 215841734U CN 202023164765 U CN202023164765 U CN 202023164765U CN 215841734 U CN215841734 U CN 215841734U
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Abstract
A liquid chromatography column relates to biomedical research and development equipment, combines an electrophoresis technology and a liquid chromatography technology, comprises a column body, an inlet joint, an outlet joint, a first electrode, a second electrode, a first electrode interface, a second electrode interface and the like, and can further separate molecules with similar molecular weights but larger charge differences, thereby improving the resolution precision of liquid chromatography.
Description
Technical Field
The utility model relates to a biomedical research and development device, in particular to a liquid chromatography column.
Background
Biopharmaceuticals are a medical industry field which is rising and developing in the twenty-first century, main products include recombinant protein drugs, monoclonal antibody drugs and the like, play irreplaceable roles in treating various major diseases such as malignant tumors, autoimmune diseases, genetic diseases, infectious diseases and the like, are developed into industries with annual output values reaching the billions dollar level at present, and have raised global development enthusiasm, and more research institutions and pharmaceutical enterprises are invested in the research and development of biopharmaceuticals.
Liquid chromatography is a widely used technology in the research and development and production processes of biological products, and can be used for identification, purity analysis, separation and purification and the like of the biological products. Liquid chromatography generally uses cross-linked sepharose gel or sephadex as a solid phase medium, and different ligands can be coupled according to needs, and can be divided into various types such as gel exclusion chromatography (also called molecular sieve), ion exchange chromatography, affinity chromatography, reverse phase chromatography and the like according to the coupling condition and technical principle of the ligands. The liquid chromatography process generally comprises filling a solid phase medium into a chromatography column, or directly purchasing a commercial pre-filled column, then pumping a buffer solution, a sample to be tested or purified into the column according to a certain sequence and flow rate by using a peristaltic pump or an automatic protein purification system, and completing the liquid chromatography through steps of balancing, loading, combining, washing, eluting and the like.
The development of biological products at present puts increasing demands on liquid chromatography technology. The advent of bispecific antibodies has further increased the need for precision in identification and isolation. Bispecific antibodies are usually in the form of heterodimers, but are often produced with many homodimeric products, which are not desired products, and their presence often interferes with the functional role of the heterodimer, and accurate detection and isolation is desirable. Because the molecular weight of the homodimer and the heterodimer is close to each other, the ordinary chromatographic technology is difficult to separate or detect, and if the chromatographic technology with higher resolution precision is available, the production preparation and quality detection of the bispecific antibody are greatly facilitated.
Disclosure of Invention
The utility model provides a liquid chromatography column, which combines an electrophoresis technology with a liquid chromatography technology and can further separate molecules with similar molecular weights but larger charge difference, thereby improving the resolution precision of liquid chromatography.
The technical scheme of the utility model is as follows:
a liquid chromatography column (figure 1) comprises a column body (1), an inlet joint (2), an outlet joint (3), a first electrode (4), a second electrode (5), a first electrode interface (6) and a second electrode interface (7); the cylinder (1) is in a hollow cylinder shape, and an inlet (10) and an outlet (11) are arranged at two ends of the cylinder; the inlet joint (2) can be hermetically connected to the inlet (10), the outlet joint (3) can be hermetically connected to the outlet (11), and the other ends of the inlet joint (2) and the outlet joint (3) can be connected to a pipeline of the liquid chromatography system; the first electrode (4) and the second electrode (5) are respectively positioned at two ends of the inner wall of the cylinder (1), and a first electrode interface (6) and a second electrode interface (7) which are communicated with the outside are arranged on the cylinder (1) and can be connected with a steady-flow voltage-stabilized power supply through a lead.
Preferably, the inner diameter of the cylinder (1) is between 1 millimeter and 1 centimeter, and the cylinder (1) is made of non-conductive glass, quartz or plastic; the first electrode (4) and the second electrode (5) are chemically stable conductive metal, such as platinum.
Preferably, the first electrode (4) and the second electrode (5) are circular thin metal sheets.
The application method of the liquid chromatography column comprises the steps that gel is poured into a column body (1) from an inlet (10), the liquid surface of the gel is covered with a first electrode (4), the column body (1) is connected to a pipeline of a liquid chromatography system through an inlet connector (2) and an outlet connector (3), and the first electrode (4) and a second electrode (5) are connected with a port of a steady-flow voltage-stabilized power supply through a first electrode interface (6) and a second electrode interface (7) respectively; after the installation and connection are completed, a liquid chromatography system is started, so that a sample to be analyzed or purified flows into the gel of the column body (1), and a steady-flow voltage-stabilized power supply is started at a proper time, so that the moving directions or speeds of molecules with different charges in the sample in the gel are different, and more accurate separation and resolution of different molecules in the sample are realized.
Compared with the common liquid chromatographic column, the utility model has higher resolution and improves the detection or purification precision.
Drawings
FIG. 1: structure of liquid chromatography column
Reference numerals: column (1), inlet joint (2), outlet joint (3), electrode I (4), electrode II (5), electrode I interface (6), electrode II interface (7), inlet (10) and outlet (11)
Detailed Description
Example 1 Structure of a liquid chromatography column
A liquid chromatography column (figure 1) comprises a column body (1), an inlet joint (2), an outlet joint (3), a first electrode (4), a second electrode (5), a first electrode interface (6) and a second electrode interface (7); the cylinder (1) is in a hollow cylinder shape, and an inlet (10) and an outlet (11) are arranged at two ends of the cylinder; the inlet joint (2) can be hermetically connected to the inlet (10), the outlet joint (3) can be hermetically connected to the outlet (11), and the other ends of the inlet joint (2) and the outlet joint (3) can be connected to a pipeline of the liquid chromatography system; the first electrode (4) and the second electrode (5) are respectively positioned at two ends of the inner wall of the cylinder (1), and a first electrode interface (6) and a second electrode interface (7) which are communicated with the outside are arranged on the cylinder (1) and can be connected with a steady-flow voltage-stabilized power supply through a lead.
The inner diameter of the cylinder (1) is between 1 millimeter and 1 centimeter, and the cylinder (1) is made of non-conductive glass; the first electrode (4) and the second electrode (5) are chemically stable conductive metal platinum. The first electrode (4) and the second electrode (5) are circular thin metal sheets.
Example 2 method of Using a liquid chromatography column
A method for using a liquid chromatography column comprises the steps of pouring gel into a column body (1) from an inlet (10), enabling the liquid surface of the gel to cover a first electrode (4), connecting the column body (1) to a pipeline of a liquid chromatography system through an inlet connector (2) and an outlet connector (3), and connecting the first electrode (4) and a second electrode (5) to a port of a steady-flow voltage-stabilized power supply through a first electrode interface (6) and a second electrode interface (7) respectively; after the connection is completed, the liquid chromatography system is started, so that the sample to be analyzed or purified flows into the gel of the column (1) from the inlet (10) and flows out of the column (1) from the outlet (11); and starting a steady-flow stabilized voltage supply at a proper time to ensure that the molecules with different charges in the sample move in different directions or different speeds in the gel, thereby realizing more accurate separation and resolution of different molecules in the sample.
Claims (4)
1. A liquid chromatography column is characterized by comprising a column body (1), an inlet joint (2), an outlet joint (3), a first electrode (4), a second electrode (5), a first electrode interface (6) and a second electrode interface (7); the cylinder (1) is in a hollow cylinder shape, and an inlet (10) and an outlet (11) are arranged at two ends of the cylinder; the inlet joint (2) can be hermetically connected to the inlet (10), the outlet joint (3) can be hermetically connected to the outlet (11), and the other ends of the inlet joint (2) and the outlet joint (3) can be connected to a pipeline of the liquid chromatography system; the first electrode (4) and the second electrode (5) are respectively positioned at two ends of the inner wall of the cylinder (1), and the outer surface of the cylinder (1) is provided with a first electrode interface (6) and a second electrode interface (7) which are communicated with the outside and can be connected with a power supply through leads.
2. A liquid chromatography column as claimed in claim 1, wherein the internal diameter of the column body (1) is between 1 mm and 1 cm.
3. A liquid chromatography column as claimed in claim 1, wherein the column body (1) is of electrically non-conductive glass, quartz or plastic material; the first electrode (4) and the second electrode (5) are made of conductive metal materials with stable chemical properties.
4. The liquid chromatography column of claim 1, wherein the first electrode (4) and the second electrode (5) are thin metal sheets having a circular ring shape.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202023164765.9U CN215841734U (en) | 2020-12-24 | 2020-12-24 | Liquid chromatography column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202023164765.9U CN215841734U (en) | 2020-12-24 | 2020-12-24 | Liquid chromatography column |
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| Publication Number | Publication Date |
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| CN215841734U true CN215841734U (en) | 2022-02-18 |
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| CN202023164765.9U Active CN215841734U (en) | 2020-12-24 | 2020-12-24 | Liquid chromatography column |
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| CN (1) | CN215841734U (en) |
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