CN215839187U

CN215839187U - Sampling swab and detection kit with same

Info

Publication number: CN215839187U
Application number: CN202121811273.6U
Authority: CN
Inventors: 徐堤
Original assignee: Jiangsu Tianjingsha Gene Diagnosis Technology Co ltd
Current assignee: Jiangsu Tianjingsha Gene Diagnosis Technology Co ltd
Priority date: 2021-08-04
Filing date: 2021-08-04
Publication date: 2022-02-18
Anticipated expiration: 2031-08-04

Abstract

The utility model discloses a sampling swab and a detection kit with the sampling swab. The swab comprises a sampling head for collecting a biological sample and a swab rod provided with a preset breaking point, the swab rod having a grip portion and a connecting portion arranged at both ends thereof. The detection kit comprises the swab and the PCR reaction tube, and the sampling head of the swab and the part of the swab rod which is positioned below the breaking point and is next to the sampling head have smaller height compared with the height of the PCR reaction tube. When the sampling head of the swab is positioned in the PCR reaction tube, the sampling head can be left in the PCR reaction tube by breaking off the swab rod of the swab at the preset breaking point, so that the tube cover of the PCR reaction tube can close the PCR reaction tube.

Description

Sampling swab and detection kit with same

Technical Field

The utility model relates to the field of medical instruments, in particular to a sampling swab and a detection kit with the sampling swab.

Background

Nucleic acid detection is currently widely used in the diagnosis of diseases, particularly diseases of the bacterial and viral infectious class. Before nucleic acid detection, samples need to be collected, and different test specimens are collected according to distribution and discharge conditions of different pathogenic bacteria in a body. The specimens are mainly blood, secretion, excrement and puncture fluid. The biological test sample is typically collected and then processed for further processing, such as nucleic acid purification and nucleic acid amplification.

Nucleic acid purification (e.g., isolation of DNA or RNA) is an important step in a variety of biochemical and diagnostic processes. The collected biological sample contains various components including contaminants (proteins, lipids, and carbohydrates) in addition to nucleic acids. The presence of contaminating substances can affect the processing of nucleic acids, for example, impurities carried over during separation can reduce yield and/or prevent the enzymatic system from synthesizing the product of interest. Therefore, it is important to efficiently separate nucleic acids from a variety of different starting materials to ensure the desired end-use functionality. Thus, it is desirable to separate nucleic acids and contaminants (proteins, lipids and carbohydrates) from a complex mixture (e.g., tissues, cells, fluids, etc.) of the sample taken therein before other processing steps (e.g., cloning, transfection, sequencing, amplification, hybridization and/or synthesis, etc.) can be performed.

The Polymerase Chain Reaction (PCR) is a molecular biology technique for amplifying a specific DNA fragment, and it has been found in experiments that DNA may be denatured and melted at high temperature and then renatured into double strands when the temperature is lowered. Therefore, the denaturation and renaturation of DNA are controlled by temperature change, and the in vitro replication of a specific gene can be completed by adding designed primers, DNA polymerase and dNTP.

After amplification, further detection is carried out, and the nucleic acid probe technology is a method which is most suitable for diagnosing bacterial infectious diseases in a method for classifying and identifying bacteria by using nucleic acid due to simple operation, high sensitivity and strong specificity.

When the collection is used for the sample of biological detection, medical staff generally adopts the mode of wearing aseptic gloves and pinching aseptic cotton swab with the hand to treat the article to be examined and carry out sample collection. The cotton swab was then cut into a sterile eluent tube with a sterile scissors for further testing. This method has the disadvantage that the sample is relatively easily contaminated.

Chinese patent (CN213430240U) discloses a medical swab, which comprises a swab head, a swab rod and a swab connecting part connecting the swab head and the swab rod, wherein: the swab head is fixed at the top end of the swab connecting part, the bottom end of the swab connecting part is connected with the top end of the swab rod, and the swab connecting part and the top end of the swab rod are connected by adopting a detachable connecting structure; the swab head comprises a swab head bracket and a flocking layer, the flocking layer is wrapped outside the swab head bracket, and the swab head is divided into a laryngeal swab head and an oral swab head. The swab head can be replaced according to the actual use condition, the assembly and disassembly are convenient, and the conversion is flexible.

Chinese patent (CN2460050) discloses a medical sampling swab, including handle, the thief rod of being connected with the handle, establish the sampling head at the thief rod front end, the handle is connected as an organic whole with the thief rod of moulding plastics, and the fibre of sampling head bonds on the thief rod, is equipped with the fracture face of being convenient for with the thief rod fracture on the thief rod. The utility model has light weight, the fiber on the sampling head is not easy to fall off, the use is convenient, the fiber can not influence the activity of bacteria, the detection result has high accuracy, and the destruction is convenient after the use.

Since the nucleic acid purification step can be omitted due to the development of the technology, the sampling head can be reacted together with the reaction tube, but the above two patents are general ones and do not consider this.

Furthermore, on the one hand, due to the differences in understanding to the person skilled in the art; on the other hand, since the inventor has studied a lot of documents and patents when making the present invention, but the space is not limited to the details and contents listed in the above, however, the present invention is by no means free of the features of the prior art, but the present invention has been provided with all the features of the prior art, and the applicant reserves the right to increase the related prior art in the background.

SUMMERY OF THE UTILITY MODEL

Aiming at the defects of the prior art, the utility model discloses a swab which at least comprises: a swab rod with a holding part and a connecting part arranged at both ends and a sampling head for sampling.

Viewed axially along the swab shaft, the cross-sectional area of the several segments that integrally form the sampling head decreases as the distance between the sampling head and the grip increases.

The outside profile of sampling head matches the inside profile of PCR reaction tube so that the sampling head can be kept by the inner wall of PCR reaction tube when falling into the PCR reaction tube to can leave the appearance chamber at PCR reaction tube's axial lower extreme when the sampling head falls into the PCR reaction tube.

The preset breaking point is arranged between the holding part and the connecting part of the swab rod, so that the sampling head can be left in the PCR reaction tube in a way of breaking at the preset breaking point on the swab rod of the swab.

The swab shaft has a grip at an end remote from the sampling head for applying a breaking force for breaking at a predetermined breaking point on the swab shaft along the swab, the swab shaft being capable of breaking at the predetermined breaking point when the grip is applied with a force.

The utility model discloses a detection kit, which comprises a swab and a PCR reaction tube, wherein a swab rod of the swab is provided with a preset breaking point. Compared with the height of the PCR reaction tube, the sampling head of the swab has smaller height together with the part of the swab rod which is positioned below the breaking point and is close to the sampling head, and when the sampling head of the swab is positioned in the PCR reaction tube, the sampling head can be left in the PCR reaction tube by breaking the breaking point on the swab rod of the swab, so that the tube cover of the PCR reaction tube can close the PCR reaction tube.

The outer contour of the sampling head of the swab is matched with the inner contour of the PCR reaction tube, the sampling head can fall into the PCR reaction tube along with the breaking along the preset breaking point and is kept by the inner wall of the PCR reaction tube, and a containing cavity is reserved at the axial lower end of the PCR reaction tube.

The swab rod is provided with a holding part at one end far away from the sampling head and used for applying breaking force for breaking at a preset breaking point on the swab rod along the swab, wherein the inner wall of the tube of the PCR reaction tube is used as a front end stress part by abutting against the sampling head of the swab, and the edge of the PCR reaction tube close to the tube opening is used as a fulcrum for breaking operation by abutting against the preset breaking point or the vicinity of the preset breaking point.

The height of the PCR reaction tube is greater than the height of the sampling head of the swab, the height of the sampling head and the height of the broken swab rod part, and the height of the sampling head and the height of the broken swab rod part plus the height of the cavity left below the sampling head in the PCR reaction tube.

The utility model has the beneficial technical effects that: the swab rod is provided with a preset breaking point, and the sampling head can be placed in the PCR reaction tube at a lower position of the preset breaking point without influencing the sealing of the tube cap so that the sampling head and the PCR reaction tube can be used for the next detection; the external contour of the sampling head of the swab rod is matched with the internal contour of the PCR reaction tube so that the PCR reaction tube can keep the sampling head to form a containing cavity, and therefore reaction liquid in the PCR reaction tube is prevented from overflowing; the swab and the reaction tube form a detection kit, so that sampling is more convenient.

Drawings

FIG. 1 is a schematic view of a swab and detection kit of the present invention;

FIG. 2 is a schematic view of a swab of the present invention incorporated into a detection kit after being snapped.

List of reference numerals

1: a swab rod 2: a sampling head 3: presetting breaking point

4: reaction tube 5: the pipe cover 11: connecting part

12: gripping part

Detailed Description

The following detailed description is made with reference to the accompanying drawings.

The utility model discloses a detection kit, which at least comprises a swab for collecting a biological sample and a PCR reaction tube, wherein the swab at least comprises: a sampling head 2 and a swab rod 1 for collecting a biological sample, wherein the swab rod 1 has a grip portion 12 for sampling grip and a connecting portion 11 for connecting the sampling head 2, which are disposed at both ends thereof.

The swab rod 1 of the swab is provided with a preset breaking point 3 so that the swab rod can be broken at the preset breaking point to separate the sampling head and the swab rod, thereby reducing the volume of the sampling device with the biological sample attached, so that the sampling head with the biological sample attached can be loaded in a closed reaction tube for further detection.

Specifically, at least part of the solid body of the swab rod is sunken along the direction of the inner wall of the swab rod to form a preset breaking point. The preset breaking point 3 is arranged between the holding part and the connecting part.

Preferably, the swab rod is provided with a plurality of preset break points so that the position of the actual break points of the swab rod can be adjusted.

The swab shaft 1 has a grip 12 at the end remote from the sampling head 2 for applying a breaking force to break along the swab shaft 1 at a predetermined breaking point 3.

When the swab rod is broken at the preset breaking point, the sampling head 2 can fall down the PCR reaction tube 4 and is held by the inner wall of the PCR reaction tube 4, and a cavity is reserved at the axial lower end of the PCR reaction tube 4.

Specifically, the cross-sectional area of a plurality of sections integrally constituting the sampling head 2 as viewed in the axial direction of the swab shaft decreases as the distance between the sampling head 2 and the grip portion 12 increases, so that the outer contour of the sampling head is substantially truncated cone-shaped to fit the inner contour of the reaction tube.

When the sampling head falls into the reaction tube, the sampling head can be supported by at least part of the inner wall of the PCR reaction tube, so that the sampling head can be just positioned at the tube cone part of the reaction tube. The sampling head and at least part of the PCR reaction tube are physically abutted to form a cavity at the lower end of the reaction tube.

Optionally, the sampling head is connected to the swab rod by means of a cover over at least part of the connection.

In particular, the sampling head is at least partially physically recessed from one end connected to the swab rod toward the opposite end to form a cavity for receiving the swab rod connection.

Optionally, the sampling head 2 is in the form of a truncated cone, and the sampling head 2 is attached to the swab rod 1 by means of a winding, foaming, or the like.

When the sampling head 2 is positioned within the PCR reaction tube, the sampling head 2 can be left within the PCR reaction tube 4 by breaking the swab shaft at a predetermined breaking point on the swab shaft 1 of the swab.

Specifically, the wiper rod 1 has a holding portion 12 at one end far from the sampling head 2 for applying a breaking force for breaking along a preset breaking point 3 on the wiper rod 1 of the swab, wherein the inner wall of the tube of the PCR reaction tube 4 acts as a front end force-bearing portion by abutting against the sampling head 2 of the swab, and the edge of the PCR reaction tube 4 close to the tube opening acts as a fulcrum for breaking operation by abutting against the preset breaking point 3 or the vicinity of the preset breaking point 3.

Specifically, when an operator applies force to the holding part, the sampling head and the swab rod holding part are subjected to force in the same direction, and due to the support of the reaction tube nozzle, one end of the swab sampling head and the part of the swab rod, which is in contact with the edge of the nozzle, are subjected to two torque actions in opposite directions, so that the swab rod is slightly deformed. The external force is applied to the wiper rod, the wiper rod generates different forces in the wiper rod because of slight deformation, wherein one side of the wiper rod, which is contacted with the edge of the pipe opening, bears extrusion force, the other side of the wiper rod bears pulling force, and the concave surfaces on the two sides of the concave at the preset breaking point are close to or far away from each other. When the force applied to the grip is sufficiently great, the predetermined breaking point is subjected to a stress exceeding its limit, and the swab shaft will break at the predetermined breaking point.

When the swab rod is broken, one end of the sampling head is not subjected to the pulling force of the swab rod along the axial direction of the swab rod any more, and the sampling head falls towards the lower end of the reaction tube along the axial direction of the reaction tube based on the gravity of the sampling head until the sampling head is supported by the inner wall of the reaction tube.

Specifically, compared with the height of the PCR reaction tube 4, the sampling head 2 of the swab and the swab rod part which is positioned below the preset breaking point and is arranged next to the sampling head 2 have smaller height, so that the tube cover 5 of the PCR reaction tube 4 can close the PCR reaction tube 4, and the pollution of the external environment to the collected biological sample is avoided.

Specifically, the height of the PCR reaction tube 4 is greater than the height of the sampling head 2 of the swab, the height of the sampling head 2 and the height of the broken swab rod part, and the height of the sampling head 2 and the height of the broken swab rod 1 part plus the height of the cavity left below the sampling head 2 in the PCR reaction tube 4.

Break the point so that the sample connection who has biological sample can be held by the reaction tube through setting up the lower position, and tube cap 5 can seal 4 messenger's reaction tubes of reaction tube simultaneously and make a closed container, avoids further operating process in to the reaction of reaction tube to cause the influence, causes water loss because of the heating if the nucleic acid amplification in-process.

The PCR reaction tube 4 is connected with a tube cover 5 for sealing the opening of the PCR reaction tube, and the tube cover 5 enables the PCR reaction tube to be a closed cavity, thereby avoiding the external environment from polluting the internal environment of the reaction tube. Meanwhile, the sampling head with the biological sample is sealed in the reaction tube to facilitate the next nucleic acid amplification, and the danger possibly caused by the fact that the biological sample carried by the sampling head is exposed to the external environment is also avoided.

The area of the diametrical plane of at least part of the tube body of the PCR reaction tube 4 is larger than the maximum cross-sectional area in the extension direction of the sampling head so that the sampling head can be inserted into the PCR reaction tube 4.

Specifically, the cross-sectional areas of the sections integrally forming the PCR reaction tube are different when the PCR reaction tube is axially observed, wherein the part of the PCR reaction tube close to the tube opening is in a straight tube shape and can be passed by a sampling head, and the part far away from the tube opening is in a tapered tube shape and can be used for holding the sampling head. When the sampling head was located the inside straight tube section of PCR reaction tube, the sampling head can deflect certain angle at the reaction tube to the body of rod that makes to wipe the son pole can support by orificial arris, and then makes the application of force in wiping the son pole so that wipe the son pole this process of breaking at predetermined breaking point more stable, avoids splashing of the biological sample who gathers.

Optionally, the PCR reaction tube is tapered.

During a particular use, a swab may be used to sample a given site. Medical personnel send the sampling head one end of swab into the whole position of waiting to detect, and the sampling head is waiting to detect the in-process of position and is accomplished the sample of treating the position at the contact. Such as a medical professional holding a swab rod and extending a sampling head attached to the swab rod into a person's mouth or nasal cavity to extract cells.

After sampling, the sampling head is separated from the part to be detected, and then one end of the swab sampling head is placed into the PCR reaction tube. If medical personnel take out the sampling head from the oral cavity or the nasal cavity of a person, the tube cover of the PCR reaction tube is opened, and the sampling head is extended into the PCR reaction tube.

After the sampling head is placed into the PCR reaction tube, the wiper rod breaks at a preset breaking point of the wiper rod by applying external force to the wiper rod based on the action of external force so as to separate the sampling head from the wiper rod. If the medical staff breaks the swab rod with a little force, one end of the sampling head falls into the reaction liquid of the PCR tube.

When the sampling head falls into the bottom of the PCR reaction tube, the sampling head can be kept by the inner wall of the reaction tube due to the fact that the outer contour of the sampling head is attached to the reaction tube, and the axial lower end of the reaction tube is provided with a containing cavity which can contain reaction liquid to avoid overflowing of the reaction liquid. The sampling head is just immersed in the reaction solution of PCR so that the sampling is sufficiently contacted with the reaction solution. The sampling head is arranged to maximally use the obtained sample for the PCR reaction.

The sampling head is placed in the PCR reaction tube and then the tube cover is closed. In general, the collected biological sample needs to be purified, but with the development of technology, this step can be omitted, and the detection method and kit for rapidly detecting the target gene by the multi-target double-dye isothermal amplification technology disclosed in patent (CN111172325A) do not need to be purified. Therefore, the sampling head with the biological sample attached does not need to be taken out of the reaction tube, and the subsequent nucleic acid amplification and detection can be carried out along with the reaction tube. Therefore, the swab disclosed by the utility model is provided with the breaking point, and the breaking point is close to the sampling head, so that the sampling head can be placed in the reaction tube, and the sealing of the tube cover on the PCR reaction tube cannot be influenced because the height of the sampling head is less than that of the reaction tube.

The detection kit and the swab disclosed by the utility model are particularly suitable for detection occasions where a nucleic acid purification step can be omitted.

It should be noted that the above-mentioned embodiments are exemplary, and that those skilled in the art, having benefit of the present disclosure, may devise various arrangements that are within the scope of the present disclosure and that fall within the scope of the utility model. It should be understood by those skilled in the art that the present specification and figures are illustrative only and are not limiting upon the claims. The scope of the utility model is defined by the claims and their equivalents.

Claims

1. A swab, comprising at least:

a wiper rod (1), wherein the wiper rod (1) is provided with a holding part (12) and a connecting part (11) which are arranged at two ends of the wiper rod;

a sampling head (2), the sampling head (2) being for collecting a biological sample,

it is characterized in that the preparation method is characterized in that,

viewed axially along the swab shaft, the cross-sectional area of several segments which form the sampling head (2) in one piece decreases with increasing distance of the sampling head (2) from the grip (12).

2. A swab according to claim 1 wherein the outer contour of the sampling head (2) matches the inner contour of a PCR reaction tube (4) such that the sampling head (2) can be held by the inner wall of the PCR reaction tube (4) when it is dropped into the PCR reaction tube (4).

3. A swab according to claim 2 wherein the sampling head (2) is adapted to leave a cavity at the lower axial end of the PCR reaction tube (4) when it is dropped into the PCR reaction tube (4).

4. A swab according to claim 3 wherein a predetermined breaking point (3) is provided between the grip portion and the connection portion of the swab shaft (1), such that the sampling head can be left inside the PCR reaction tube (4) by breaking at said predetermined breaking point on the swab shaft (1) of the swab.

5. A swab according to claim 4 wherein the swab stem (1) has a grip (12) at the end remote from the sampling head (2) for exerting a breaking force to break along the swab stem (1) at the predetermined breaking point (3).

6. A swab according to claim 5 wherein the swab stem (1) is adapted to break at said predetermined breaking point when a force is applied to the grip portion.

7. A detection kit, which comprises a swab and a PCR reaction tube, is characterized in that,

the swab rod (1) of the swab is provided with a preset breaking point (3),

the swab sampling head (2) together with the portion of the swab stem (1) located immediately below the break point, which is located next to the sampling head (2), has a smaller height than the height of the PCR reaction tube (4),

wherein, when the sampling head (2) of the swab is positioned in the PCR reaction tube, the sampling head (2) can be left in the PCR reaction tube (4) by breaking at the preset breaking point on the swab rod (1) of the swab, so that the tube cover (5) of the PCR reaction tube (4) can close the PCR reaction tube (4).

8. The detection kit according to claim 7, wherein the outer contour of the sampling head (2) of the swab matches the inner contour of the PCR reaction tube (4),

the sampling head (2) can fall into the PCR reaction tube (4) along with the breakage of the preset breaking point (3) and is kept by the inner wall of the PCR reaction tube (4), and a containing cavity is reserved at the axial lower end of the PCR reaction tube (4).

9. The detection kit according to claim 7, wherein the swab rod (1) has a grip (12) at an end remote from the sampling head (2) for applying a breaking force for breaking along the predetermined breaking point (3) on the swab rod (1), wherein the inner wall of the PCR reaction tube (4) acts as a front end force receiving portion by abutting against the swab head (2) of the swab, and the edge of the PCR reaction tube (4) close to the mouth of the tube acts as a fulcrum for the breaking operation by abutting against the predetermined breaking point (3) or the vicinity of the predetermined breaking point (3).

10. The test kit according to claim 7, wherein the height of the PCR reaction tube (4) is greater than the height of the sampling head (2) of the swab, and is also greater than the height of the sampling head (2) together with the height of the portion of the swab stem (1) after breakage plus the height of the cavity left in the PCR reaction tube (4) below the sampling head (2).