CN215811818U

CN215811818U - Sampling device for flow cytometry detection

Info

Publication number: CN215811818U
Application number: CN202122400743.6U
Authority: CN
Inventors: 杨文山
Original assignee: Shandong Amber Biotechnology Co ltd
Current assignee: Shandong Amber Biotechnology Co ltd
Priority date: 2021-10-02
Filing date: 2021-10-02
Publication date: 2022-02-11
Anticipated expiration: 2031-10-02

Abstract

The utility model discloses a sampling device for flow cytometry detection, and relates to the field of cell detection. This sampling device that flow cytometry used includes the workstation, the carousel, preforming and inserted bar, the protruding axle of workstation fixedly connected with, protruding axle fixedly connected with connecting axle, the rectangular hole has been seted up to the connecting axle, workstation fixedly connected with connecting seat, workstation fixedly connected with connecting cylinder, swing joint has movable ejector pin in the connecting cylinder, movable ejector pin fixedly connected with spring, the carousel rotates to be connected on the connecting axle, the carousel is provided with a plurality of recess, the carousel has been seted up a plurality of and has been contacted with the ring of placing of movable ejector pin, preforming fixedly connected with rectangular plate, rectangular plate sliding connection is in the rectangular hole, inserted bar swing joint is in the connecting seat. The sampling device for flow cytometry detection has a function of facilitating sampling of different cell stock solutions contained in a plurality of test tubes.

Description

Sampling device for flow cytometry detection

Technical Field

The utility model relates to the technical field of cell detection, in particular to a sampling device for flow cytometry detection.

Background

Flow cytometric sampling devices are devices that can automatically analyze and sort cells and can rapidly measure, store and display a series of important biophysical and biochemical characteristic parameters of dispersed cells suspended in a liquid.

The present invention discloses a sampling device for flow cytometry, which comprises a sampling tube for sucking a cell stock solution, wherein the cell stock solution is generally placed in a test tube, and when the cell stock solution to be sampled is too many types, a user needs to manually replace the test tube to sample different types of cell stock solutions.

SUMMERY OF THE UTILITY MODEL

Technical problem to be solved

The utility model discloses a sampling device for flow cytometry detection, aiming at overcoming the defects in the prior art.

(II) technical scheme

In order to achieve the purpose, the utility model is realized by the following technical scheme: a sampling device for flow cytometry comprising:

the upper surface of the workbench is fixedly connected with a convex shaft, the upper surface of the convex shaft is fixedly connected with a connecting shaft, the upper surface of the connecting shaft is provided with a rectangular hole, the upper surface of the workbench is fixedly connected with a connecting seat, the upper surface of the workbench is fixedly connected with a connecting cylinder, a movable ejector rod is movably connected in the connecting cylinder, and the bottom of the movable ejector rod is fixedly connected with a spring;

the rotary table is rotationally connected to the connecting shaft, a plurality of grooves are formed in the upper surface of the rotary table, and a plurality of placing rings in contact with the movable ejector rods are formed in the lower surface of the rotary table;

the lower surface of the pressing sheet is fixedly connected with a rectangular plate, and the rectangular plate is connected in a rectangular hole in a sliding manner;

the inserted bar is movably connected in the connecting seat.

Preferably, the protruding shaft is arranged at a position close to the center of the workbench, the connecting cylinder is arranged at a position close to the front surface of the workbench, and the connecting seat is arranged at a position close to one side of the workbench.

Preferably, connecting axle and protruding axle integrated into one piece cast shaping, the rectangular hole runs through to the protruding epaxial in, first jack has all been seted up to the both sides in rectangular hole, and first jack all runs through to the excircle surface of protruding axle.

Preferably, one side of the rectangular plate is provided with a second jack corresponding to the first jack, and the second jack penetrates through the other side of the rectangular plate.

Preferably, place ring and recess and all set up in the position that is close to carousel excircle surface, place the quantity of ring and recess and set up to equal, a plurality of places the ring and is in under a plurality of recess respectively.

Preferably, the outer circle surface of the inserted bar is fixedly connected with a limiting ring, and the limiting ring is positioned between the convex shaft and the connecting seat.

The utility model discloses a sampling device for flow cytometry detection, which has the following beneficial effects:

1. this sampling device that flow cytometry used, through setting up the workstation, the carousel, preforming and inserted bar, the test tube that is equipped with different cell stoste is put in a plurality of recesses, the carousel rotates to be connected on the connecting axle, insert the rectangular plate in the rectangular hole, later insert in first jack and the second jack with the inserted bar, elasticity and the activity ejector pin through the spring and the cooperation of placing the ring, can carry out certain location to the position of every test tube, the cell stoste of test tube is conveniently sampled in every recess, the whole sample backs of test tube on the carousel are accomplished, remove the inserted bar and make and break away from with first jack and second jack, just can change the carousel that has put new test tube on the protruding axle, the device has the function that can be convenient for carry out the sample to the different cell stoste that are equipped with in a plurality of test tubes.

2. This sampling device that flow cytometry used, through setting up the spacing ring, when changing the carousel, the inserted bar removes to the direction of keeping away from the protruding axle, after the spacing ring contacts with the connecting seat, the inserted bar has broken away from with the second jack, but the inserted bar still is in the first jack that is close to the connecting seat, rotate when new carousel and connect on the connecting axle after, just can insert the inserted bar in the second jack again fast, can change the carousel sooner, the device has the function that can change the carousel sooner.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic structural diagram of a workbench according to the present invention;

FIG. 3 is a schematic structural diagram of the turntable of the present invention;

FIG. 4 is a schematic view of the structure of a wafer according to the present invention;

FIG. 5 is a schematic view of the structure of the insert rod of the present invention.

In the figure: 1. a work table; 101. a protruding shaft; 102. a connecting shaft; 103. a rectangular hole; 104. a first jack; 105. a connecting seat; 106. a connecting cylinder; 107. a movable ejector rod; 108. a spring; 2. a turntable; 201. placing a ring; 202. a groove; 3. tabletting; 301. a rectangular plate; 302. a second jack; 4. inserting a rod; 401. a limit ring.

Detailed Description

The embodiment of the utility model discloses a sampling device for flow cytometry detection, which comprises a workbench 1, a turntable 2, a pressing sheet 3 and an insertion rod 4:

according to fig. 1 and 2, the upper fixed surface of the workbench 1 is connected with a protruding shaft 101, the protruding shaft 101 is arranged at a position close to the center of the workbench 1, the upper fixed surface of the protruding shaft 101 is connected with a connecting shaft 102, a rectangular hole 103 is formed in the upper surface of the connecting shaft 102, the connecting shaft 102 and the protruding shaft 101 are integrally cast and formed, the rectangular hole 103 penetrates into the protruding shaft 101, first jacks 104 are formed in two sides of the rectangular hole 103, the first jacks 104 penetrate through the outer circle surface of the protruding shaft 101, and the positions of the first jacks 104 correspond to the connecting seat 105.

According to the illustration of fig. 2, the upper surface of the workbench 1 is fixedly connected with a connecting seat 105, the connecting seat 105 is arranged at a position close to one side of the workbench 1, the inserting rod 4 is movably connected in the connecting seat 105, and the inserting rod 4 can move left and right in the connecting seat 105.

According to the attached drawing of fig. 2, the upper surface of the working table 1 is fixedly connected with a connecting cylinder 106, the connecting cylinder 106 is arranged at a position close to the front surface of the working table 1, a movable ejector rod 107 is movably connected in the connecting cylinder 106, the bottom of the movable ejector rod 107 is fixedly connected with a spring 108, the top of the movable ejector rod 107 is hemispherical, the movable ejector rod 107 can move up and down in the spring 108, and the movable ejector rod 107 always tends to move up under the elastic action of the spring 108.

According to the attached

drawing

1, 2 and 3 show, carousel 2 rotates to be connected on connecting axle 102, the upper surface of carousel 2 is provided with a plurality of recess 202, the lower surface of carousel 2 is seted up a plurality of and is contacted with activity ejector pin 107 place ring 201, place ring 201 and recess 202 and all set up the position near 2 excircle surfaces of carousel, it all is even annular distribution about the centre of a circle of carousel 2 to place ring 201 and recess 202, the quantity of placing ring 201 and recess 202 sets up to equal, a plurality of places ring 201 and is in under a plurality of recess 202 respectively, the test tube that is equipped with different cell stoste needs to be put in recess 202.

As shown in fig. 1, 2 and 4, a rectangular plate 301 is fixedly connected to the lower surface of the pressing sheet 3, the rectangular plate 301 is slidably connected in the rectangular hole 103, a second insertion hole 302 corresponding to the first insertion hole 104 is formed in one side of the rectangular plate 301, the second insertion hole 302 penetrates through the other side of the rectangular plate 301, and when the insertion rod 4 is inserted into the first insertion hole 104 and the second insertion hole 302, the pressing sheet 3 is fixed, so that the rotary table 2 can only rotate on the connecting shaft 102 and cannot move upwards.

The device is through setting up workstation 1, carousel 2, preforming 3 and inserted bar 4, the test tube that is equipped with different cell stoste is put in a plurality of

recesses

202, 2 rotation of carousel are connected on connecting axle 102, insert rectangular plate 301 in rectangular hole 103, later insert first jack 104 and second jack 302 with inserted bar 4 in, elasticity and activity ejector pin 107 through spring 108 and the cooperation of placing ring 201, can carry out certain location to the position of every test tube, the cell stoste of test tube is conveniently sampled in every recess 202, the whole back of accomplishing of taking a sample of test tube on carousel 2, it makes and breaks away from with first jack 104 and second jack 302 to remove inserted bar 4, just can change carousel 2 that has put new test tube on protruding axle 101, the device has the function that can be convenient for carry out the sample to the different cell stostes that are equipped with in a plurality of test tubes.

According to fig. 5, a limiting ring 401 is fixedly connected to the outer circumferential surface of the insertion rod 4, the diameter of the limiting ring 401 is larger than that of the insertion rod 4, the limiting ring 401 is located between the protruding shaft 101 and the connecting seat 105, when the insertion rod 4 is completely separated from the second insertion hole 302, the limiting ring 401 abuts against one side of the connecting seat 105 close to the protruding shaft 101, and the insertion rod 4 is still located in the first insertion hole 104 close to the connecting seat 105.

The device is through setting up spacing ring 401, when changing carousel 2, inserted bar 4 is to keeping away from the direction removal of protruding axle 101, after spacing ring 401 and connecting seat 105 contact, inserted bar 4 has broken away from with second jack 302, but inserted bar 4 still is in being close to in the first jack 104 of connecting seat 105, after new carousel 2 rotates to be connected on connecting axle 102, just can be fast with inserted bar 4 reinsertion in the second jack 302, can change carousel 2 sooner, the device has the function that can change carousel 2 sooner.

The working principle is as follows: the device is when using, put a plurality of test tubes that are equipped with the cell stoste one by one in recess 202, later with the carousel 2 that has a plurality of test tubes rotate to be connected on connecting axle 102, make the lower surface of carousel 2 and the upper surface laminating of protruding axle 101, later with the slip grafting of rectangular plate 301 in rectangular hole 103, make the lower surface of preforming 3 and the upper surface laminating of carousel 2 mutually, second jack 302 just corresponds with first jack 104 this moment, the user removes the inserted bar 4 in the connecting seat 105 to the direction that is close to protruding axle 101, make inserted bar 4 pass second jack 302 and first jack 104, fix inserted bar 4 like this, carousel 2 only can rotate and can't reciprocate again on connecting axle 102.

The movable push rod 107 is movably connected in the connecting cylinder 106, the movable push rod 107 always tends to move upwards under the elastic action of the spring 108, after the rotary disc 2 is rotatably connected on the connecting shaft 102, when the placing ring 201 is not contacted with the movable push rod 107, the rotary disc 2 presses the movable push rod 107 to enable the movable push rod 107 to be completely contracted in the connecting cylinder 106, when the rotary disc 2 rotates to enable any placing ring 201 to correspond to the movable push rod 107, the movable push rod 107 pushes the placing ring 201 under the elasticity of the spring 108, so that resistance can be applied to the rotation of the rotary disc 2 to play a certain fixing effect on the rotary disc 2, a user can feel the resistance, after the movable push rod 107 pushes one placing ring 201, the user samples cell stock solution in the test tube in the groove 202 above the placing ring 201, and then immediately rotates the rotary disc 2 to enable the movable push rod 107 to push the next placing ring 201 anticlockwise again, can carry out certain location to the position of every test tube like this, convenience of customers carries out the sample of cell stoste to the test tube in next recess 202.

After the test tubes on the rotary table 2 are all subjected to cell stock solution sampling, the inserted bar 4 is separated from the first insertion hole 104 and the second insertion hole 302, the rotary table 2 is moved upwards, then the new rotary table 2 with the test tubes is rotationally connected onto the connecting shaft 102, and the cell stock solution sampling is carried out again after the steps are repeated.

At the excircle fixed surface of inserted bar 4 and be connected with spacing ring 401, the diameter of spacing ring 401 is greater than inserted bar 4, when changing carousel 2, only need inserted bar 4 and second jack 302 and one of them first jack 104 break away from completely can, the user stimulates inserted bar 4 to make inserted bar 4 keep away from protruding axle 101, just break away from the back completely with second jack 302 as inserted bar 4, spacing ring 401 will offset with one side that connecting seat 105 is close to protruding axle 101, inserted bar 4 still is in being close to in the first jack 104 of connecting seat 105 this moment, rotate the connection back on connecting axle 102 with new carousel 2 like this, can insert inserted bar 4 in second jack 302 again fast, thereby can accomplish the change to carousel 2 sooner, the device has the function that can change carousel 2 sooner.

The foregoing shows and describes the general principles and broad features of the present invention and advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the utility model as claimed. The scope of the utility model is defined by the appended claims and equivalents thereof.

Claims

1. A sampling device for flow cytometry, comprising:

the automatic lifting device comprises a workbench (1), wherein a convex shaft (101) is fixedly connected to the upper surface of the workbench (1), a connecting shaft (102) is fixedly connected to the upper surface of the convex shaft (101), a rectangular hole (103) is formed in the upper surface of the connecting shaft (102), a connecting seat (105) is fixedly connected to the upper surface of the workbench (1), a connecting cylinder (106) is fixedly connected to the upper surface of the workbench (1), a movable ejector rod (107) is movably connected into the connecting cylinder (106), and a spring (108) is fixedly connected to the bottom of the movable ejector rod (107);

the rotary table (2) is rotatably connected to the connecting shaft (102), a plurality of grooves (202) are formed in the upper surface of the rotary table (2), and a plurality of placing rings (201) which are in contact with the movable ejector rods (107) are formed in the lower surface of the rotary table (2);

the lower surface of the pressing sheet (3) is fixedly connected with a rectangular plate (301), and the rectangular plate (301) is connected in the rectangular hole (103) in a sliding mode;

the inserting rod (4), the inserting rod (4) is movably connected in the connecting seat (105).

2. A sampling device for flow cytometry detection as described in claim 1, wherein: protruding axle (101) set up in the position that is close to workstation (1) center, connecting cylinder (106) set up in the position that is close to workstation (1) front, connecting seat (105) set up in the position that is close to workstation (1) one side.

3. A sampling device for flow cytometry detection as described in claim 1, wherein: connecting axle (102) and protruding axle (101) integrated casting shaping, rectangular hole (103) run through to protruding axle (101) in, first jack (104) have all been seted up to the both sides of rectangular hole (103), first jack (104) all run through to the excircle surface of protruding axle (101).

4. A sampling device for flow cytometry detection as described in claim 3, wherein: and one side of the rectangular plate (301) is provided with a second jack (302) corresponding to the first jack (104), and the second jack (302) penetrates through the other side of the rectangular plate (301).

5. A sampling device for flow cytometry detection as described in claim 1, wherein: the placing ring (201) and the groove (202) are arranged at positions close to the outer circle surface of the turntable (2), the number of the placing ring (201) and the number of the groove (202) are set to be equal, and the placing ring (201) is respectively positioned under the grooves (202).

6. A sampling device for flow cytometry detection as described in claim 1, wherein: the outer circle surface of the inserted bar (4) is fixedly connected with a limiting ring (401), and the limiting ring (401) is located between the protruding shaft (101) and the connecting seat (105).