CN2158085Y - Fluorescent diagnostic meter for tumor - Google Patents

Abstract

The utility model relates to a fluorescent diagnostic meter for tumors, which belongs to a weak fluorescent high precision measuring instrument. The fluorescent diagnostic meter for tumors can rapidly, accurately and non-destructively diagnose tumor diseases. The design eliminates red sector radiation and uses diaphragms to regulate and inspire light intensity; a square sample pool with large upper caliber and small lower caliber, and a rotary type wave chopping slit are designed; the signal-to-noise ratio of the fluorescence signal collection is enhanced; the zero drift caused by the change of the dark current of a photomultiplier tube is eliminated. The utility model has very high measuring precision and accuracy.

Description

Fluorescent diagnostic meter for tumor

The design belongs to a kind of utmost point hypofluorescence high precision measuring instrument, and this instrument is mixed the diagnosing tumor expert system software, can be fast, accurately, diagnosing tumour with no damage.

Usually the fluorescence spectrophotometer that adopts is when carrying out the measurement of the high precision utmost point low light level at present, and accuracy rate and signal to noise ratio (S/N ratio) all are difficult to meet the demands.F-4000 spectral fluorometer as HIT's production, its structure is as excitation source by a continuous xenon lamp, by convex lens exciting light focusing is entered light beam split monochromator slit, and be radiated at and carry out beam split on the spheric grating, the light of being told is through exit slit, shine on the sample cell from the side by convex lens again, sample fluoresce is collected the entrance slit that enters the fluorescence monochromator through convex lens, spheric grating beam split through monochromator, the fluorescence of being told impinges upon on the photomultiplier by the monochromator exit slit, the electric signal of photomultiplier output forms fluorescence spectrum by amplifying circuit and computer interface by Computer Processing.Because the universal design (promptly can measure excitation spectrum and can measure emission spectrum again) of this class spectrometer, make it in the measurement of high precision utmost point hypofluorescence, be subjected to certain qualification, as xenon lamp is that full bands of a spectrum are luminous, general spectral fluorometer is in order to carry out exciting light scanning, with the luminous full illumination of full bands of a spectrum of lamp on the light spectro-grating, because the heat radiation that ruddiness produces causes the little distortion of grating, thereby the position that has influenced exciting light is accurate.In the design of F-4000 fluorescence spectrophotometer, the variation of excitation light intensity is to divide the way of optical slits size to realize with change light, but has just changed the live width of exciting light like this, thereby has changed the quality of exciting light, has influenced the measuring accuracy of fluorescence spectrum.

The purpose of this utility model provides a kind of instrument of high precision utmost point hypofluorescence spectral measurement.

To achieve these goals, the design adopts following technical scheme:

Anti-red convex lens of purple and saturating red anti-purple concave mirror are housed respectively in the front and back of exciter lamp, the collimation back is carried out intensity adjustments by an iris to exciting light, behind interference filter or grating beam splitting, make exciting light become vertical direction by two plane mirrors, and make the relative monochromator entrance slit of two luminous strong points of lamp become fore-and-aft direction, thereby strengthened launching efficiency.Exciting light is focused in the sample cell from the top by a plano-convex lens, forms a vertical fluorescence bright line and parallel with vertical slit in sample cell, and slit designs is rotary copped wave slit.Lamp house is eliminated the red sector radiation, regulates excitation light intensity with diaphragm.Change two strong spots of light source into fore-and-aft direction from above-below direction.Sample cell is designed to bore and descends the little square sample cell of bore greatly.

More than design has been to improve the signal to noise ratio (S/N ratio) of system, has eliminated because the photomultiplier dark current changes the zero drift that causes, and makes this instrument that great improvement arranged in the measurement of high precision utmost point hypofluorescence signal, has improved the precision and the accuracy of measuring.

Below in conjunction with drawings and Examples the design is described in further detail.

Fig. 1 is the design's a block diagram.

1-excites lamp house among the figure; The 2-sample chamber; 3-exciter lamp power supply; 4-cooling fan power supply; 5-fluorescence monochromator; The 6-high-voltage power supply; The 7-signal amplification circuit; The 8-stepper motor driver; The 9-computer interface; The 10-computing machine; The 11-printer.

Fig. 2 is lamp house and sample chamber synoptic diagram.

The saturating red anti-purple concave mirror of 12-among the figure; 13-three-dimensional regulation lampshade; Saturating red anti-purple concave mirror is a plano-concave lens, and the saturating red anti-purple membrane of concave surface plating, the luminescent center of the concave surface centre of sphere and lamp coincide and be contained in the rear of three-dimensional regulation lampshade; The 14-exciter lamp can be with xenon lamp or high-pressure sodium lamp, and it is excitation source that present embodiment adopts xenon lamp; The saturating purple anti-red plano-convex lens of 15-is installed in the place ahead of regulating lampshade, at the saturating purple anti-red film of flat one side plating; 16-interference filter horse can be installed the optical filter of 6 different wave lengths; 17-catoptron micropositioning stage; The 18-fine adjustment screw; The 19-plane mirror, this catoptron is contained on the micropositioning stage, by the direction of fine adjustment screw accommodation reflex mirror; The 20-adjustable diaphragm is regulated beam diameter by this diaphragm and is changed excitation light intensity; The 21-beam splitter is installed with incident ray angle at 45, and its transmitance is 90%; The 22-light intensity monitors photoelectric tube, accepts the light of being told by beam splitter; 23-exciting light focus lamp is plano-convex lens; 24-constant temperature sample chamber; The 25-well heater; The 26-thermal detector; The 27-sample cell; The 28-concave mirror; The 29-biconvex lens; The rotary copped wave slit of 30-; Plano-convex lens is contained on the upper cover plate of sample chamber, be that last bore descends the little square sample cell of bore greatly under it, plano-convex lens focuses on the sample from the top with exciting light, the heavy caliber of top can not block the exciting light from the top, and the bottom is that the few sample of small-bore feasible amount can keep certain height.Concave mirror is the sphere total reflective mirror, and reflectance coating is plated on the concave surface, and installation requirement is the centre of its centre of sphere at photoluminescence line.Well heater and thermal detector are housed on the sidewall of constant temperature sample chamber.Biconvex lens is contained in the middle of sample cell and the slit.

Fig. 3 is rotary copped wave slit synoptic diagram.

31-light emitting diode among the figure is installed in slit respective sample pond one side; The 32-photodiode is installed in the corresponding opposite side of slit; The 33-shadow shield; The 34-bearing.Be fixed on the shadow shield; The synchronous hole of 35-; 36-leads to optical slits; The 37-tumbler; The 38-servomotor; The 39-axle sleeve.The last lower shaft of tumbler is contained in the bearing respectively.Hole and slit are opened on tumbler synchronously, and slit is the middle long perforate of tumbler.Motor shaft links to each other with the lower shaft of tumbler with axle sleeve.

Open exciter lamp 14 before the measurement earlier, because the effect of catoptron 12 and collimating mirror 15 makes the direction outgoing of useful purple light towards catoptron 19, heat radiation is outgoing in the opposite direction.Exciting light behind the collimation becomes extremely narrow line spectrum behind spike interference filter.Catoptron 19 is transferred 90 ° light beam on surface level, but two strong spots in lamp source still are 2 points (two electrodes of lamp are above-below direction) up and down, catoptron 19 becomes 90 ° with the downward corner of light beam, and make two strong spots in lamp source become front and back position (with respect to slit), so just the light intensity of accepting with slit has increased by one times.Plano-convex lens 23 focuses on excitation beam in the sample cell 27, because the design of sample cell makes the upper surface of sample (serum) form concave surface bended, produced the concave mirror effect, this concave surface makes the light beam that is converged by plano-convex lens 23 become parallel fine rule, in sample cell, produced a vertical fluorescence fine rule, this fine rule is parallel with slit 30, converges through biconvex lens 29, enters the monochromator slit and penetrates on inside the spheric grating with certain relative aperture.Concave mirror 28 reflects the fluorescence of other direction, enters slit 30 through lens 29.Motor 38 drives tumblers 37 and rotates, and when forwarding a certain angle to, the logical optical slits 36 on the tumbler is over against sample cell, and fluorescence sees through slit and enters monochromator, and the light that sends of light emitting diode 31 enters photodiode 32 through synchronous hole 35 simultaneously, produces synchronizing signal.Slit is closed when tumbler 37 changes 90 ° again, and the hole is also closed synchronously, and fluorescence is produced copped wave, and after processing and amplifying, deliver to microcomputer 10 and handle the formation fluorescence spectrum as calculated by machine interface 9 through lock for the signal after the copped wave.

Claims (1)

1, a kind of fluorescence diagnosing tumor instrument, by lamp house, the sample chamber, monochromator and computing machine are formed, and it is characterized in that:

A. respectively there are saturating purple anti-red convex lens of one side and saturating red anti-purple concavees lens in the front and back of exciter lamp;

B. the plane mirror of lamp house is installed on the micropositioning stage;

C., one adjustable diaphragm is arranged before the beam splitter;

D. sample cell is that last bore descends the little Fang Chi of bore greatly;

E. the side at sample cell has a concave mirror, and reflectance coating is plated on the concave surface of this catoptron;

F., one biconvex lens is arranged in the middle of sample cell and the slit;

G. slit is the middle long perforate of tumbler, and slit counter sample pond one side has a light emitting diode, and corresponding opposite side has a photodiode;

H. the last lower shaft of tumbler is contained in respectively in two bearings on the plate washer, and its lower shaft links to each other with servomotor with axle sleeve.

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