CN215757374U - Digital PCR micro-fluidic chip temperature measurement module - Google Patents
Digital PCR micro-fluidic chip temperature measurement module Download PDFInfo
- Publication number
- CN215757374U CN215757374U CN202121755882.4U CN202121755882U CN215757374U CN 215757374 U CN215757374 U CN 215757374U CN 202121755882 U CN202121755882 U CN 202121755882U CN 215757374 U CN215757374 U CN 215757374U
- Authority
- CN
- China
- Prior art keywords
- temperature
- microfluidic chip
- micro
- measurement module
- fluidic chip
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The utility model provides a digital PCR micro-fluidic chip temperature measurement module, which comprises a micro-fluidic chip, wherein at least two sample adding holes are distributed on the micro-fluidic chip, the sample adding holes are communicated through a runner inner cavity arranged in the micro-fluidic chip, at least two grooves are formed in the micro-fluidic chip, and temperature sensors are arranged in the grooves. The utility model can simultaneously measure the real-time temperature of different point positions; the temperature value is recorded in real time, so that the temperature change rule is convenient to observe, and the temperature data is visually displayed in an image form; in addition, the utility model ensures that the temperature sensor is closely contacted with the measured point, and improves the stability and accuracy of the measured data.
Description
Technical Field
The utility model belongs to the technical field of temperature measurement of PCR chips, and particularly relates to a temperature measurement module of a PCR microfluidic chip.
Background
Microfluidics is a technique for the precise control and manipulation of microscale fluids, which possesses the characteristics of small volume (nanoliters, picoliters, femtoliters) and low energy consumption. People use the microfluidic technology to integrate basic operation units of sample preparation, reaction, separation, detection and the like in the processes of biological, chemical and medical analysis on a microfluidic chip taking a microfluidic pipeline as a basic structure, so that the advantages of rapid sample treatment and detection, small reagent and sample usage amount and the like are realized. Due to its great potential in the fields of biology, chemistry, medicine and the like, the method has been developed into a new research field crossing the disciplines of biology, chemistry, medicine, fluid, electronics, materials, machinery and the like.
Digital PCR, a nucleic acid quantification method based on a single-molecule PCR method for counting, is an absolute quantification method. The principle is that a large amount of diluted nucleic acid solution is dispersed into a micro-reactor or a micro-droplet of a chip by a micro-fluidic or micro-droplet method, and the number of nucleic acid templates in each reactor is less than or equal to 1. After PCR cycling, a reactor or microdroplet with a nucleic acid molecule template will give a fluorescent signal, and a reactor or microdroplet without a template will not. The nucleic acid concentration of the original solution was deduced from the relative proportion and the volume of the reactor.
The existing temperature measurement technology can be roughly divided into a contact type and an infrared type, the contact type achieves the purpose of measuring the temperature by contacting a heat conduction metal rod with a measured object, the mode can only measure the temperature of a single position, can only measure the temperature value at a certain moment, cannot continuously measure the temperature change within a period of time, and does not have the capability of data recording; the infrared temperature measurement can measure the temperature in one area, but the measurement precision is low, the error is large, and the infrared temperature measurement device does not have the data recording function generally.
Disclosure of Invention
In order to solve the defects of the prior art, the utility model provides a digital PCR micro-fluidic chip temperature measurement module.
The purpose of the utility model is realized by the following technical scheme:
the utility model provides a digital PCR micro-fluidic chip temperature measurement module, includes the micro-fluidic chip, it has two at least sample application holes to distribute on the micro-fluidic chip, through set up in between the sample application hole the inside runner inner chamber UNICOM of micro-fluidic chip, two at least recesses have been seted up on the micro-fluidic chip, be provided with temperature sensor in the recess.
Preferably, the microfluidic chip further comprises a movable pressing block arranged above the microfluidic chip.
Preferably, the groove extends from the end part of the microfluidic chip to the other end of the microfluidic chip to a temperature measuring point.
Preferably, the temperature sensor is correspondingly arranged in a groove at the end part of the inner cavity of the flow channel.
Preferably, the temperature sensor is also correspondingly arranged in a groove in the middle of the inner cavity of the flow channel.
Preferably, the bottom surface of the movable pressing block is abutted against the upper surface of the microfluidic chip, and the area of the bottom surface of the movable pressing block covers the temperature sensor.
Preferably, the bottom surface of the movable pressing block is provided with a heat insulation layer cushion.
Preferably, the temperature sensor is electrically connected with an external temperature reader.
The utility model has the beneficial effects that: real-time temperatures of different point locations can be measured simultaneously; the temperature sensors are not interfered with each other, so that the accuracy of data can be ensured; the temperature reading instrument records the temperature value in real time, so that the temperature change rule can be conveniently observed, and the temperature data can be visually presented in an image form; in addition, the design of the movable pressing block ensures that the temperature sensor is closely contacted with the measured point, and the stability of the measured data is improved.
Drawings
FIG. 1: the structure of the micro-fluidic chip of the utility model.
FIG. 2: the utility model is a schematic structural diagram of a movable pressing block.
FIG. 3: the utility model relates to the position relation between the inner cavity of a micro-fluidic chip and a temperature measuring point.
FIG. 4: the utility model relates to a peripheral temperature reader.
1-microfluidic chip, 11-sample adding hole, 12-groove, 14-temperature measuring point, 13-flow channel inner cavity, 2-movable pressing block, 21-heat insulation cushion layer and 3-liquid crystal display screen.
Detailed Description
The technical scheme of the utility model is specifically explained below by combining with the embodiment and fig. 1-4, and the utility model discloses a digital PCR micro-fluidic chip temperature measurement module, which comprises a micro-fluidic chip 1, wherein a plurality of sample adding holes 11 are convexly distributed on the micro-fluidic chip 1, the sample adding holes 11 comprise oil holes and sample holes, and the sample adding holes 11 are communicated through a flow channel inner cavity 13 arranged in the micro-fluidic chip 1, so that the sample adding holes are not polluted by the outside during reaction.
The micro-fluidic chip is provided with at least two grooves 12, the grooves 12 extend from the end part of the micro-fluidic chip 1 to the other end of the micro-fluidic chip 1 to a temperature measuring point, and the grooves 12 are mutually independent and are not communicated with each other.
Temperature sensors are arranged in the groove 12, specifically, the temperature sensors 14 are arranged at a temperature point 14 to be measured, and the temperature sensors are distributed at the end part of the flow channel inner cavity 13 and the middle part of the flow channel inner cavity. The microfluidic chip 1 is made of a heat insulating material, so that the temperatures between the grooves 12 do not affect each other, and the accuracy of measured data is ensured.
In addition, micro-fluidic chip 1 top sets up movable briquetting 2, the bottom surface of movable briquetting 2 with the upper surface butt of micro-fluidic chip 1, just the bottom surface of movable briquetting 2 sets up thermal-insulated layer pad 21, in this embodiment, thermal-insulated layer pad 21 material is bubble silica gel pad for prevent that micro-fluidic chip 1 heat transfer from running off. The bottom surface area of the movable pressing block 2 covers the temperature sensor and is used for pressing the temperature sensor tightly, so that the temperature sensor is in close contact with the temperature measuring point 14, and the stability of data is ensured.
In this embodiment, the application of sample hole be provided with 6, set up as required respectively in microfluidic chip's both ends, the application of sample hole at both ends is through four inside runner inner chamber 13 UNICOMs, for the temperature in each region of better survey runner inner chamber 13, this embodiment corresponds the tip and the middle part of runner inner chamber 13 respectively and sets up temperature sensor, because temperature sensor passes through the wire and is connected with outside temperature reading instrument, the event adopts microfluidic chip surface is seted up flutedly, the distal end of recess is the temperature point 14 that awaits measuring, arranges temperature sensor in the temperature point 14 that awaits measuring, and its wire can be arranged in the recess and extend microfluidic chip and be connected with outside temperature measuring reading instrument. The arrangement of the groove can provide a good accommodating position for the lead, and meanwhile, the pressing of the movable pressing block 2 on the surface of the microfluidic chip cannot be influenced. The tortuous shape of the groove 12 on the microfluidic chip is not limited, and the shortest path to the temperature measuring point and the full utilization of the space on the chip are preferred.
The upper part and the lower part of the microfluidic chip 1 are both provided with heating components, the chip of the microfluidic chip 1 is heated when necessary, the area of the heating components is generally 5 to 100 percent larger than the heating area required by the microfluidic chip 11 for effective heat conduction, the heating component at the upper part is removed when temperature measurement is carried out,
. The temperature measurement reading instrument is conventional and generally comprises a sensor value reading port, a liquid crystal display screen 3 and built-in temperature recording software, temperature values of different temperature measurement points are recorded in real time, the temperature change rule is convenient to observe, and obtained data are displayed visually in an image form.
In conclusion, the utility model can simultaneously measure the real-time temperatures of different point locations; the temperature sensors are not interfered with each other, so that the accuracy of data can be ensured; the temperature value is recorded in real time, so that the temperature change rule is convenient to observe, and the temperature data is visually displayed in an image form; in addition, the design of the movable pressing block ensures that the temperature sensor is closely contacted with the measured point, and the stability of the measured data is improved.
There are, of course, many other specific embodiments of the utility model and these are not to be considered as limiting. All technical solutions formed by using equivalent substitutions or equivalent transformations fall within the scope of the claimed invention.
Claims (8)
1. The temperature measurement module of the digital PCR microfluidic chip is characterized by comprising the microfluidic chip, wherein at least two sample adding holes are distributed on the microfluidic chip and communicated with each other through a flow channel inner cavity arranged in the microfluidic chip, at least two grooves are formed in the microfluidic chip, and temperature sensors are arranged in the grooves.
2. The digital PCR microfluidic chip temperature measurement module according to claim 1, wherein: the device also comprises a movable pressing block arranged above the microfluidic chip.
3. The digital PCR microfluidic chip temperature measurement module according to claim 1, wherein: the groove extends from the end part of the micro-fluidic chip to the other end of the micro-fluidic chip to a temperature measuring point.
4. The digital PCR microfluidic chip temperature measurement module according to claim 3, wherein: the temperature sensor is correspondingly arranged in the groove at the end part of the inner cavity of the flow channel.
5. The digital PCR microfluidic chip temperature measurement module according to claim 4, wherein: the temperature sensor is also correspondingly arranged in the groove in the middle of the inner cavity of the flow channel.
6. The digital PCR microfluidic chip temperature measurement module according to claim 2, wherein: the bottom surface of the movable pressing block is abutted to the upper surface of the microfluidic chip, and the area of the bottom surface of the movable pressing block covers the temperature sensor.
7. The digital PCR microfluidic chip temperature measurement module according to claim 2, wherein: the bottom surface of the movable pressing block is provided with a heat insulation layer cushion.
8. The digital PCR microfluidic chip temperature measurement module according to claim 1, wherein: the temperature sensor is electrically connected with a peripheral temperature reading instrument.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202121755882.4U CN215757374U (en) | 2021-07-30 | 2021-07-30 | Digital PCR micro-fluidic chip temperature measurement module |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202121755882.4U CN215757374U (en) | 2021-07-30 | 2021-07-30 | Digital PCR micro-fluidic chip temperature measurement module |
Publications (1)
Publication Number | Publication Date |
---|---|
CN215757374U true CN215757374U (en) | 2022-02-08 |
Family
ID=80108389
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202121755882.4U Active CN215757374U (en) | 2021-07-30 | 2021-07-30 | Digital PCR micro-fluidic chip temperature measurement module |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN215757374U (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2023159910A1 (en) * | 2022-02-23 | 2023-08-31 | 常州先趋医疗科技有限公司 | Temperature measurement apparatus and method in lamp |
-
2021
- 2021-07-30 CN CN202121755882.4U patent/CN215757374U/en active Active
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2023159910A1 (en) * | 2022-02-23 | 2023-08-31 | 常州先趋医疗科技有限公司 | Temperature measurement apparatus and method in lamp |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US20230101252A1 (en) | Apparatus and methods for performing electrochemical reactions | |
US11536710B2 (en) | Droplet microfluidic device and methods of sensing the result of an assay therein | |
JP3947536B2 (en) | Method and apparatus for measuring specimen in liquid | |
CN110205242A (en) | It is a kind of to fast implement the micro-fluidic chip component and its application that digital pcr reacts | |
EP2021103B1 (en) | Electrowetting droplet microactuator controlled via graphical user interface | |
US7822510B2 (en) | Systems, methods, and products for graphically illustrating and controlling a droplet actuator | |
CA2439924C (en) | Analysis device | |
CN215757374U (en) | Digital PCR micro-fluidic chip temperature measurement module | |
CN107923914A (en) | The method of drop microfluidic device and sensing measurement result therein | |
Jayamohan et al. | Advances in microfluidics and lab-on-a-chip technologies | |
CN205170857U (en) | A micro -fluidic chip , detecting system and device for DNA short -term test | |
CN107884456A (en) | Detect the sensor and method and the system of control sample preparation of fluid state | |
CN202415561U (en) | Quantitative PCR (Polymerase Chain Reaction) microfluidic control chip device | |
US9733174B2 (en) | Capillary microviscometer | |
CN107074526A (en) | The micro-fluid chip sensed for blood coagulation | |
Trauba et al. | Microfluidic extreme PCR:< 1 minute DNA amplification in a thin film disposable | |
CN111394219A (en) | Integrated digital PCR system | |
CN1117282C (en) | PCR microarray probe circulating detection type biological chip | |
CN210596085U (en) | Microfluidic chip assembly for rapidly realizing digital PCR reaction and integrated equipment thereof | |
JP7434266B2 (en) | How to measure hematocrit in a fluidic channel that includes a conductivity sensor | |
WO2013130995A1 (en) | Lateral flow analysis system, method and article | |
CN202814969U (en) | Disposable type consumable for matched handheld type rapid detection analysis device | |
CN218596406U (en) | Nucleic acid constant temperature amplification colloidal gold detection device | |
CN201034871Y (en) | Micro-miniature vapor bubble and spume eliminating device with platinum resistor temperature measurement | |
US20100181211A1 (en) | Electrochemical sensor and biosensor and method of electrochemical measurement |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
GR01 | Patent grant | ||
GR01 | Patent grant |