CN215743524U

CN215743524U - Microsphere fluorescent microfluidic chip

Info

Publication number: CN215743524U
Application number: CN202121933829.9U
Authority: CN
Inventors: 乔杰; 冯建梅
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-08-18
Filing date: 2021-08-18
Publication date: 2022-02-08
Anticipated expiration: 2031-08-18

Abstract

The utility model relates to the technical field of fluorescence microfluidics, and discloses a microsphere fluorescence microfluidic chip, which comprises: the spheroid shell, spheroid shell inner chamber fixedly connected with substrate, upper left end position fixedly connected with microballon of substrate, microballon top fixedly connected with sample import pipe, the fixed solution export of having seted up in microballon bottom, solution export bottom fixedly connected with solution runner pipe, solution runner pipe right side wall fixedly connected with solution shunt tubes, solution shunt tubes right side fixedly connected with fluorescent tube, fluorescent tube right side fixed connection is on the detection tube. According to the utility model, a detection sample enters the inner cavity of the microsphere through the sample inlet pipe and is fused with a detection reagent in the inner cavity of the microsphere, and the fused solvent is caused to flow into one or more solution shunt pipes by adjusting the micro valves according to the actually required detection types through external force shaking, so that the sample and the detection reagent can be fully fused, the subsequent detection accuracy is greatly improved, and the method is practical and suitable for wide popularization and use.

Description

Microsphere fluorescent microfluidic chip

Technical Field

The utility model relates to the technical field of fluorescent microfluidics, in particular to a microsphere fluorescent microfluidic chip.

Background

The chip applying the immunofluorescence detection technology at present has the immunochromatography and the fluorescence immune microfluidic technology. Compared with the prior art, the immunochromatography method has the advantages that the immunochromatography method is limited by the defects of the manufacturing process of a multilayer structure, and has the problems of large batch-to-batch difference and large batch-to-batch difference, the single-layer structure of the immune microfluidic chip technology can effectively reduce the influence factors of the test result of the chip, the control on the batch-to-batch difference and the batch-to-batch difference is enhanced, the immune microfluidic chip technology greatly reduces the sample consumption, saves the labor and time cost, can realize the advantages of automation, high-flux experiments and the like in a centimeter square space, and is widely concerned.

The existing immune microfluidic chip adopts single-channel liquid inlet reaction, and has the problem of signal crosstalk when a plurality of items are detected, so that the application range of the chip is limited. The existing multi-channel multi-target immunofluorescence chip has the defects that a sample to be detected and a detection reagent are fused unevenly, subsequent detection effects are influenced, impurities are prone to appearing in fusion liquid of the sample to be detected and the detection reagent, a solution flow pipe is prone to being blocked, and circulation of the solution is influenced.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a microsphere fluorescent microfluidic chip, which solves the problems that the subsequent detection effect is influenced due to the uneven fusion of a sample to be detected and a detection reagent, and the solution circulation is influenced due to the blockage of a solution circulation pipe caused by the easy occurrence of impurities in the fusion liquid of the sample to be detected and the detection reagent in the background technology.

In order to achieve the purpose, the utility model provides the following technical scheme: a microsphere fluorescent microfluidic chip comprising: the spheroid shell, the fixed substrate of having placed of spheroid shell inner chamber, the upper left end position fixedly connected with microballon of substrate, microballon top fixedly connected with sample import pipe, the fixed solution export of having seted up in microballon bottom, solution export bottom fixedly connected with solution runner pipe, solution runner pipe right side wall fixedly connected with solution shunt tubes, solution shunt tubes right side fixedly connected with fluorescent tube, fluorescent tube right side fixed connection is on the test tube.

In a preferred embodiment of the present invention, a fixed amount of the detection reagent is fixedly placed in the inner cavity of the microsphere.

By adopting the technical scheme, the detected sample can be fully fused with the detection reagent in the microsphere, and the fused reagent enters a detection area for detection, so that the comprehensiveness and integrity of detection data are improved.

As a preferred embodiment of the present invention, a filter screen is fixedly welded to the bottom of the solution outlet at the lower end of the microsphere.

By adopting the technical scheme, impurities in the fused liquid can be fully filtered, the accuracy of subsequent detection numerical values can be increased, and the phenomenon that the impurities block the pipeline to influence the working flow of the chip can be avoided.

In a preferred embodiment of the present invention, a micro valve is fixedly connected to a junction of the solution flowing tube and the solution dividing tube.

Through adopting above-mentioned technical scheme, can come control solution to flow in the quantity of solution shunt tubes through adjusting the microvalve according to the detection kind of actual demand, increase its work efficiency.

In a preferred embodiment of the present invention, the waste liquid flow tube is screwed to the right side of the detection tube, and the end of the waste liquid flow tube is fixedly connected to the waste liquid collection box.

Through adopting above-mentioned technical scheme, detect the waste liquid after accomplishing and flow into the waste liquid and collect the box in, prevent the waste liquid backward flow, reduce the influence of waste liquid backward flow to the measured data.

As a preferred embodiment of the utility model, the inner cavity wall of the microsphere is movably connected with an arc-shaped blocking plate.

By adopting the technical scheme, the arc-shaped blocking plate is operated, so that the sample and the detection reagent can be prevented from flowing into the detection pipeline when the sample and the detection reagent are not completely fused.

In a preferred embodiment of the present invention, the sample inlet tube is fixedly provided with a pinhole at a middle position.

By adopting the technical scheme, the detected object in the needle cylinder can uniformly and completely flow into the microsphere from the needle hole on the sample inlet pipe in a pressing mode, and after the sample and the detection reagent are fused, the air pressure in the needle cylinder is pressed, so that the air pressure flows into a subsequent pipeline from the needle hole on the sample inlet pipe, and the flow of the fusion agent is accelerated.

Compared with the prior art, the utility model has the following beneficial effects:

1. in the specific working process of the microsphere fluorescent microfluidic chip, a detection sample enters an inner cavity of a microsphere through a sample inlet pipe and contacts with a detection reagent of the microsphere, a microvalve is adjusted according to actually required detection types by the pressure pressed by a needle cylinder, so that the solvent flows into one or more solution shunt pipes, the solution flows and is fused in the shunt pipes, the fused solvent flows into the fluorescent pipe for fluorescent coating, the solvent coated with the fluorescent solvent then flows into the detection pipe for detection, and the solvent in contact with the detection flows into a waste liquid collection box through a waste liquid circulation pipe, so that the sample and the detection reagent can be fully fused, and the accuracy of subsequent detection is greatly improved.

2. In the specific working process of the microsphere fluorescent microfluidic chip, after a detection sample and a detection reagent are fully fused, the arc-shaped blocking plate is lifted, so that a solution flows into the solution outlet, and the solution flowing into the solution outlet is filtered by the filter screen and flows into the solution flow pipe, so that the impurities of the solution are filtered, the quality of the solution is improved, and the accuracy of subsequent detection data is improved.

Drawings

Other features, objects and advantages of the utility model will become more apparent upon reading of the detailed description of non-limiting embodiments with reference to the following drawings:

FIG. 1 is a schematic diagram of a cross-sectional structure of a microsphere fluorescent microfluidic chip according to the present invention;

FIG. 2 is a schematic diagram of a substrate structure of a microsphere fluorescent microfluidic chip according to the present invention;

FIG. 3 is a schematic diagram of a microsphere structure of a microsphere fluorescence microfluidic chip according to the present invention;

FIG. 4 is a schematic diagram of a side view of a sample inlet tube of a microsphere fluorescence microfluidic chip according to the present invention.

In the figure: 1. a spherical shell; 2. microspheres; 3. a sample inlet tube; 4. a solution circulation pipe; 5. a micro valve; 6. a solution shunt tube; 7. a fluorescent tube; 8. a detection tube; 9. a waste liquid circulation pipe; 10. a waste liquid collection box; 11. filtering with a screen; 12. a solution outlet; 13. an arc-shaped blocking plate; 14. a substrate; 15. a pinhole.

Detailed Description

In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the utility model easy to understand, the utility model is further described with the specific embodiments.

In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.

The first embodiment is as follows:

referring to fig. 1-4, the present invention provides a technical solution: a microsphere fluorescent microfluidic chip comprising: the detection device comprises a sphere shell 1, a substrate 14 is fixedly placed in an inner cavity of the sphere shell 1, a microsphere 2 is fixedly connected to the left upper end of the substrate 14, a sample inlet pipe 3 is fixedly connected to the top of the microsphere 2, a fixed amount of detection reagent is fixedly placed in an inner cavity of the microsphere 2, a detected sample can be fully fused with the detection reagent in the microsphere 2, the fused reagent enters a detection area to be detected, and comprehensiveness and integrity of detection data are improved.

Referring to fig. 1-4, a solution outlet 12 is fixedly formed in the bottom of the microsphere 2, a solution flow pipe 4 is fixedly connected to the bottom of the solution outlet 12, a solution flow dividing pipe 6 is fixedly connected to the right side wall of the solution flow pipe 4, a fluorescent tube 7 is fixedly connected to the right side of the solution flow dividing pipe 6, the fluorescent tube 7 is fixedly connected to the right side of the detection tube 8, so that the solution can smoothly flow into the fluorescent tube 7 for coating, and the solution after coating can make the detection data more accurate.

Referring to fig. 1-4, a filter screen 11 is fixedly welded at the bottom of a solution outlet 12 at the lower end of the microsphere 2, and an arc-shaped blocking plate 13 is movably connected to the inner cavity wall of the microsphere, so that impurities in the fused solution can be fully filtered, the accuracy of subsequent detection values can be improved, the phenomenon that the pipeline is blocked by the impurities to influence the work flow of a chip can be avoided, and the sample and a detection reagent can be prevented from flowing into the detection pipeline when the sample and the detection reagent are not completely fused by operating the arc-shaped blocking plate 13.

Referring to fig. 1-4, a pinhole 15 is fixedly formed in the middle of the sample inlet tube 3, so that a detected object in the syringe can uniformly and completely flow into the microspheres from the pinhole on the sample inlet tube by pressing, and after the sample and the detection reagent are fused, the air pressure in the syringe can flow into a subsequent pipeline from the pinhole on the sample inlet tube by pressing the air pressure in the syringe, so as to accelerate the flow of the fusion reagent.

The implementation principle of the microsphere fluorescent microfluidic chip in the first embodiment of the application is as follows: in the specific working process, a detection sample enters an inner cavity of a microsphere 2 through a sample inlet pipe 3 and contacts with a detection reagent in the microsphere 2, a contacted solvent is enabled to regulate a micro valve 5 according to the detection type required by the practice through the pressure pressed by a needle cylinder, the solvent flows into one or more solution shunt pipes 6 to enable the solution to circulate and fuse in the shunt pipes 6, the fused solvent flows into a fluorescent tube 7 to carry out fluorescent coating, the solvent coated with fluorescent light then flows into a detection tube 8 to carry out detection, the solvent in contact with the detection flows into a waste liquid collection box 10 through a waste liquid circulation pipe 9, the sample and the detection reagent can be enabled to be fully fused, and the accuracy of subsequent detection is greatly improved; in the concrete working process, after the detection sample and the detection reagent are fully fused, through opening arc-shaped blocking plate 13, the solution flows into solution outlet 12, the solution flowing into solution outlet 12 is filtered by filter screen 11 and flows into solution flow pipe 4, the impurities in the solution are filtered, the quality of the solution is improved, and the accuracy of subsequent detection data is improved.

Example two:

referring to fig. 1 to 4, a micro valve 5 is fixedly connected to a connection portion of the solution flowing pipe 4 and the solution dividing pipe 6, and the number of the solution flowing into the solution dividing pipe can be controlled by adjusting the micro valve according to the detection type of the actual requirement, so as to increase the working efficiency.

Referring to fig. 1-4, the right side thread of the detection tube 8 is screwed with the waste liquid circulation tube 9, the tail end of the waste liquid circulation tube 9 is fixedly connected to the waste liquid collection box 10, and the detected waste liquid flows into the waste liquid collection box to prevent the waste liquid from flowing back, so that the influence of the waste liquid flowing back on detection data is reduced, the waste liquid can be treated in a centralized manner, and the pollution to the environment is avoided.

It should be noted that the present invention is a microsphere fluorescence microfluidic chip, each of which is a general standard component or a component known to those skilled in the art, and the structure and principle of the chip can be known to those skilled in the art through a technical manual or through a routine experimental method.

While there have been shown and described what are at present considered the fundamental principles and essential features of the utility model and its advantages, it will be apparent to those skilled in the art that the utility model is not limited to the details of the foregoing exemplary embodiments, but is capable of other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the utility model being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims

1. A microsphere fluorescence micro-fluidic chip is characterized in that: the method comprises the following steps: spheroid shell (1), fixed substrate (14) having placed of spheroid shell (1) inner chamber, upper left end position fixedly connected with microballon (2) of substrate (14), microballon (2) top fixedly connected with sample inlet pipe (3), solution export (12) have been seted up to microballon (2) bottom is fixed, solution export (12) bottom fixedly connected with solution runner pipe (4), solution runner pipe (4) right side wall fixedly connected with solution shunt tubes (6), solution shunt tubes (6) right side fixedly connected with fluorescent tube (7), fluorescent tube (7) right side fixed connection is on detecting tube (8).

2. The microsphere fluorescent microfluidic chip of claim 1, wherein: and a fixed amount of detection reagent is fixedly placed in the inner cavity of the microsphere (2).

3. The microsphere fluorescent microfluidic chip of claim 1, wherein: a filter screen (11) is fixedly welded at the bottom of a solution outlet (12) at the lower end of the microsphere (2).

4. The microsphere fluorescent microfluidic chip of claim 1, wherein: the connecting part of the solution flowing pipe (4) and the solution shunt pipe (6) is fixedly connected with a micro valve (5).

5. The microsphere fluorescent microfluidic chip of claim 1, wherein: the right side screw thread of detection pipe (8) is twisted on and is had waste liquid runner pipe (9), and waste liquid runner pipe (9) end fixed connection is on waste liquid collection box (10).

6. The microsphere fluorescent microfluidic chip of claim 1, wherein: the inner cavity wall of the microsphere (2) is movably connected with an arc-shaped plugging plate (13).

7. The microsphere fluorescent microfluidic chip of claim 1, wherein: a pinhole (15) is fixedly arranged in the middle of the sample inlet pipe (3).