CN215727178U - Cell precipitation experimental device for research of forced spondylitis osteogenesis differentiation mechanism - Google Patents
Cell precipitation experimental device for research of forced spondylitis osteogenesis differentiation mechanism Download PDFInfo
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- CN215727178U CN215727178U CN202122053793.1U CN202122053793U CN215727178U CN 215727178 U CN215727178 U CN 215727178U CN 202122053793 U CN202122053793 U CN 202122053793U CN 215727178 U CN215727178 U CN 215727178U
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- 230000007246 mechanism Effects 0.000 title claims abstract description 38
- 238000001556 precipitation Methods 0.000 title claims abstract description 20
- 201000002661 Spondylitis Diseases 0.000 title claims abstract description 14
- 230000011164 ossification Effects 0.000 title claims abstract description 6
- 230000004069 differentiation Effects 0.000 title abstract description 6
- 238000004062 sedimentation Methods 0.000 claims abstract description 6
- 230000009818 osteogenic differentiation Effects 0.000 claims description 10
- 238000007789 sealing Methods 0.000 claims description 5
- 230000002146 bilateral effect Effects 0.000 claims description 2
- 206010002556 Ankylosing Spondylitis Diseases 0.000 claims 2
- 210000004027 cell Anatomy 0.000 description 20
- 238000000034 method Methods 0.000 description 5
- 238000003756 stirring Methods 0.000 description 5
- 239000000758 substrate Substances 0.000 description 4
- 210000001124 body fluid Anatomy 0.000 description 2
- 239000010839 body fluid Substances 0.000 description 2
- 210000000988 bone and bone Anatomy 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 210000002569 neuron Anatomy 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 210000003050 axon Anatomy 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 230000002380 cytological effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000010827 pathological analysis Methods 0.000 description 1
- 210000000578 peripheral nerve Anatomy 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 208000007442 rickets Diseases 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 208000020431 spinal cord injury Diseases 0.000 description 1
- 210000001032 spinal nerve Anatomy 0.000 description 1
- 230000000946 synaptic effect Effects 0.000 description 1
- 210000002435 tendon Anatomy 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 208000037816 tissue injury Diseases 0.000 description 1
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Abstract
The utility model discloses a cell precipitation experimental device for research of forced spondylitis osteogenesis differentiation mechanism, and particularly relates to the technical field of cell precipitation. According to the utility model, the sliding seat is driven to move inwards by rotating the second threaded rod, the test tube is clamped and fixed on the inner ring plate, so that the test tube can rotate along with the inner ring plate, then the first threaded rod is driven to rotate by the micro positive and negative motor, the movable plate, the inner ring plate and the outer ring plate are driven to move up and down by the first threaded rod, the inner ring plate and the outer ring plate are connected through the bearing, the inner ring plate can rotate by means of the matching of the positioning column and the threaded channel while moving up and down along with the movable plate, the test tube is driven to rotate by the inner ring plate while rotating, and the sedimentation of cells is accelerated by driving the test tube to rotate up and down in the integral structure.
Description
Technical Field
The utility model relates to the technical field of cell precipitation, in particular to a cell precipitation experimental device for research on an osteogenic differentiation mechanism of forced spondylitis.
Background
Spinal cord injury is one of the clinically common serious diseases, has presented the situation of high incidence, high disability rate, high consumption and low aging in the world, and becomes a great medical problem to be solved urgently in the medical field. How to rescue the damaged neurons, promote the axon regeneration of the damaged neurons and form correct synaptic connections with target tissues, and the recovery of spinal nerve functions, etc. are the key points and hot spots of the current research. The biological scaffold material product taking collagen as the matrix is already approved to be used for clinical treatment of tissue injury repair of blood coagulation, burn, bone, tendon, peripheral nerve and the like.
Centrifugation and sedimentation tubes are common in medical experiments, and are usually used for collecting as many cells as possible, and the requirement of intact cell structure is an important condition for the pathological diagnosis and cytological study of body fluid cells. The collection of cells from body fluids is carried out in common test tubes, and the cells precipitated at the bottom of the common test tube are dug out by a small spoon for smear no matter the gravity precipitation or the centrifugal precipitation method is used.
The existing cell precipitation device has the problems of inconvenient operation and use and poor centrifugal stirring effect, the stirring mode of the device stirs cells through a motor-driven stirring rod, and the cells are damaged by contact with the cells in the stirring process, so that a cell precipitation experimental device for forced rachitis osteogenesis differentiation mechanism research is needed.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the technical problem of providing a cell precipitation experimental device for research on an osteogenic differentiation mechanism of forced spondylitis.
In order to achieve the purpose, the utility model provides the following technical scheme: the cell sedimentation experimental device for research on the forced spondylitis osteogenesis differentiation mechanism comprises a base plate, wherein two fixed plates are fixedly arranged at the top of the base plate, the two fixed plates are arranged in bilateral symmetry around the central axis of the base plate, grooves are formed in the sides, close to the two fixed plates, of the two fixed plates, an adjusting mechanism and a limiting mechanism are respectively arranged in the grooves, the adjusting mechanism is located on one side of the limiting mechanism, two movable plates are arranged between the two fixed plates, an outer ring plate is fixedly arranged between the two movable plates, an inner ring plate is arranged in the outer ring plate, the outer wall of the inner ring plate is movably connected with the inner wall of the outer ring plate through a bearing, a test tube is arranged in the inner ring plate, a sealing plug is arranged in the top end of the test tube, a clamping mechanism is fixedly arranged on the rear side of the top end of the inner ring plate, a threaded channel is formed in the outer wall of the inner ring plate, and a positioning column is arranged in the bottom end of the threaded channel, the locating column rear side is equipped with the backup pad, backup pad bottom and base plate top fixed connection, backup pad top front side and locating column rear end fixed connection.
In a preferred embodiment, the adjusting mechanism includes a first threaded rod, the first threaded rod is located inside a groove on one of the fixing plates, the top end and the bottom end of the first threaded rod are respectively movably connected with the top and the bottom of an inner cavity of the groove through bearings, a movable plate located on one side of the outer ring plate close to the first threaded rod is sleeved outside the first threaded rod, and the movable plate is in threaded connection with the first threaded rod.
In a preferred embodiment, the movable plate and the groove are both provided with a rectangular cross section.
In a preferred embodiment, the limiting mechanism comprises a guide rod, the guide rod is located inside a groove on one of the fixed plates, the top end and the bottom end of the guide rod are respectively and fixedly connected with the top and the bottom of an inner cavity of the groove, and a movable plate located on one side of the outer ring plate close to the guide rod is sleeved outside the guide rod.
In a preferred embodiment, a miniature positive and negative motor is fixedly arranged at the top of the fixing plate outside the first threaded rod, and the output end of the miniature positive and negative motor penetrates through the fixing plate and is fixedly connected with the top end of the first threaded rod.
In a preferred embodiment, fixture includes the diaphragm, the diaphragm is located interior crown of roll top rear side, the fixed connecting plate that is equipped with in diaphragm front side, connecting plate and interior crown of roll top rear side fixed connection, the spacing groove has been seted up at the diaphragm top, the spacing inslot portion is equipped with the second threaded rod, second threaded rod both ends all through the bearing respectively with spacing groove both sides inner wall swing joint, the equal threaded connection in second threaded rod both ends outer wall has the slide.
In a preferred embodiment, the thread directions of two ends of the second threaded rod are opposite, the threads of two ends of the second threaded rod are symmetrically arranged about the central axis of the second threaded rod, and the cross sections of the sliding seat and the limiting groove are both rectangular.
In a preferred embodiment, the test tube top both sides all are equipped with the grip block, the grip block contacts with test tube one side, grip block rear end and slide top front side fixed connection.
The utility model has the technical effects and advantages that: according to the utility model, the sliding seat is driven to move inwards by rotating the second threaded rod, the test tube is clamped and fixed on the inner ring plate, so that the test tube can rotate along with the inner ring plate, then the micro positive and negative motor drives the first threaded rod to rotate, the first threaded rod simultaneously drives the movable plate, the inner ring plate and the outer ring plate to move up and down, and the outer ring plate and the inner ring plate are connected through the bearing, so that the inner ring plate can rotate by means of the matching of the positioning column and the threaded channel while moving up and down along with the movable plate, the inner ring plate also drives the test tube to rotate while rotating, and the whole structure accelerates the precipitation of cells by driving the test tube to rotate while moving up and down.
Drawings
Fig. 1 is a front view of the overall structure of the present invention.
Fig. 2 is a rear view of the overall structure of the present invention.
Fig. 3 is a cross-sectional view of a fixation plate of the present invention.
Fig. 4 is an exploded view of the positioning post and inner ring plate of the present invention.
FIG. 5 is a clamping view of the cross plate and the inner ring plate of the present invention.
FIG. 6 is a top view of the cross plate of the present invention.
The reference signs are: 1. a substrate; 2. a fixing plate; 3. a groove; 4. a movable plate; 5. an outer ring plate; 6. an inner ring plate; 7. a test tube; 8. a sealing plug; 9. a threaded channel; 10. a positioning column; 11. a support plate; 12. a first threaded rod; 13. a guide bar; 14. a micro positive and negative motor; 15. a transverse plate; 16. a connecting plate; 17. a limiting groove; 18. a second threaded rod; 19. a slide base; 20. and (4) clamping the plate.
Detailed Description
Referring to the attached drawings 1-6 of the specification, the cell precipitation experimental device for research on the osteogenic differentiation mechanism of the forced spondylitis of the embodiment comprises a substrate 1, two fixed plates 2 are fixedly arranged on the top of the substrate 1, the two fixed plates 2 are symmetrically arranged on the left and right sides of the central axis of the substrate 1, grooves 3 are respectively formed on the adjacent sides of the two fixed plates 2, an adjusting mechanism and a limiting mechanism are respectively arranged in the two grooves 3, the adjusting mechanism is positioned on one side of the limiting mechanism, two movable plates 4 are arranged between the two fixed plates 2, an outer ring plate 5 is fixedly arranged between the two movable plates 4, an inner ring plate 6 is arranged in the outer ring plate 5, the outer wall of the inner ring plate 6 is movably connected with the inner wall of the outer ring plate 5 through a bearing, a test tube 7 is arranged in the inner ring plate 6, a sealing plug 8 is arranged in the top of the test tube 7, and a clamping mechanism is fixedly arranged on the rear side of the top end of the inner ring plate 6, the outer wall of the inner ring plate 6 is provided with a thread channel 9, a positioning column 10 is arranged inside the bottom end of the thread channel 9, a supporting plate 11 is arranged on the rear side of the positioning column 10, the bottom end of the supporting plate 11 is fixedly connected with the top of the base plate 1, the front side of the top end of the supporting plate 11 is fixedly connected with the rear end of the positioning column 10, a sliding seat 19 is driven to move inwards by rotating a second threaded rod 18, the test tube 7 is clamped and fixed on the inner ring plate 6, the test tube 7 can rotate along with the inner ring plate 6, then a micro positive and negative motor 14 drives a first threaded rod 12 to rotate, the first threaded rod 12 drives the movable plate 4, the inner ring plate 6 and the outer ring plate 5 to move up and down simultaneously, the inner ring plate 6 is connected with the inner ring plate 6 through a bearing while moving up and down along with the movable plate 4, can rotate by means of the cooperation of the positioning column 10 and the thread channel 9, and the inner ring plate 6 also drives the test tube 7 to rotate while rotating, the whole structure accelerates the sedimentation of cells by driving the test tube 7 to move up and down and simultaneously rotate.
The adjusting mechanism comprises a first threaded rod 12, the first threaded rod 12 is positioned inside a groove 3 on one of the fixed plates 2, the top end and the bottom end of the first threaded rod 12 are respectively movably connected with the top and the bottom of an inner cavity of the groove 3 through bearings, a movable plate 4 positioned on one side of the outer ring plate 5 close to the first threaded rod 12 is sleeved outside the first threaded rod 12, the movable plate 4 is in threaded connection with the first threaded rod 12, the movable plate 4 limited by the groove 3 is driven to move up and down when the first threaded rod 12 rotates, the movable plate 4 simultaneously drives the inner ring plate 6 and the outer ring plate 5 to move up and down, the sections of the movable plate 4 and the groove 3 are both rectangular, the groove 3 and the movable plate 4 are rectangular, so that the movable plate 4 can perform vertical linear motion in the motion process, and a micro positive and negative motor 14 is fixedly arranged at the top of the fixed plate 2 positioned outside the first threaded rod 12, the output end of the micro positive and negative motor 14 penetrates through the fixing plate 2 and is fixedly connected with the top end of the first threaded rod 12, the micro positive and negative motor 14 drives the first threaded rod 12 to rotate, the first threaded rod 12 drives the movable plate 4 limited by the groove 3 to move up and down when rotating, the whole structure is operated by the micro positive and negative motor 14, the use is simple, the clamping mechanism comprises a transverse plate 15, the transverse plate 15 is positioned at the rear side of the top end of the inner annular plate 6, a connecting plate 16 is fixedly arranged at the front side of the transverse plate 15, the connecting plate 16 is fixedly connected with the rear side of the top end of the inner annular plate 6, a limiting groove 17 is formed in the top of the transverse plate 15, a second threaded rod 18 is arranged in the limiting groove 17, two ends of the second threaded rod 18 are respectively and movably connected with the inner walls at two sides of the limiting groove 17 through bearings, the outer walls at two ends of the second threaded rod 18 are both in threaded connection with a sliding seat 19, and the sliding seat 19 limited by the limiting groove 17 is driven to move inwards or outwards simultaneously when the second threaded rod 18 rotates, when the test tube is moved outwards, the slide seat 19 drives the clamping plate 20 to separate from the test tube 7, the test tube 7 is not clamped and fixed, when the test tube is moved inwards, the slide seat 19 drives the clamping plate 20 to clamp and fix the test tube 7, so that the test tube 7 can rotate along with the inner annular plate 6, the screw directions of the two ends of the second threaded rod 18 are opposite, the two ends of the second threaded rod 18 are symmetrically arranged about the central axis of the second threaded rod 18, the cross sections of the slide seat 19 and the limiting groove 17 are both rectangular, the second threaded rod 18 also drives the two slide seats 19 thereon to move inwards or outwards simultaneously when rotating, and drives the clamping plate 20 to clamp and fix or disassemble and separate the test tube 7 during moving, the clamping plate 20 is arranged on both sides of the top end of the test tube 7, the clamping plate 20 is in contact with one side of the test tube 7, the rear end of the clamping plate 20 is fixedly connected with the front side of the top end of the slide seat 19, and through the arrangement of the clamping plate 20, it is fixed to be convenient for carry out the centre gripping to test tube 7 to be fixed in the ring plate 6 with test tube 7 on, make test tube 7 rotate along with interior ring plate 6.
The implementation scenario is specifically as follows: the user places the material inside test tube 7, and seals test tube 7 through sealing plug 8, then through rotating second threaded rod 18, make second threaded rod 18 drive two slide 19 limited by spacing groove 17 and move inwards at the same time, fix test tube 7 centre gripping on interior crown plate 6, start miniature positive and negative motor 14 at last, drive first threaded rod 12 and rotate by miniature positive and negative motor 14, first threaded rod 12 drives when rotating and receives the spacing fly leaf 4 of recess 3 and reciprocates, also drive interior crown plate 6 and outer crown plate 5 and reciprocate when fly leaf 4 reciprocates, because connect through the bearing between outer crown plate 5 and the interior crown plate 6, the inner ring plate 6 can rotate by means of the matching of the positioning column 10 and the thread groove 9 while moving up and down with the movable plate 4, and inner ring plate 6 drives test tube 7 to rotate when rotating, and overall structure carries out the formula of going up and down through driving test tube 7 and rotates the sediment that accelerates the cell.
Referring to the attached drawing 3 of the specification, the cell sedimentation experimental apparatus for research on bone differentiation mechanism of forced spondylitis of this embodiment comprises a guide rod 13, the guide rod 13 is located inside the groove 3 of one of the fixed plates 2, the top end and the bottom end of the guide rod 13 are fixedly connected with the top and the bottom of the inner cavity of the groove 3 respectively, the movable plate 4 located on the outer ring plate 5 side close to the guide rod 13 is sleeved outside the guide rod 13, and the movable position of the movable plate 4 can be limited by the arrangement of the guide rod 13.
The implementation scenario is specifically as follows: when first threaded rod 12 drives interior crown plate 6 and outer crown plate 5 through fly leaf 4 and moves, guide bar 13 has also carried on spacingly to the removal of interior crown plate 6 and outer crown plate 5, avoids interior crown plate 6 and outer crown plate 5 to take place the skew at the in-process that removes to improve the stability of interior crown plate 6 and outer crown plate 5 at the removal in-process.
Claims (8)
1. Forced spondylitis osteogenesis is differential mechanism research and is used cell sedimentation experimental apparatus, includes base plate (1), its characterized in that: the test tube comprises a base plate (1), wherein two fixed plates (2) are fixedly arranged at the top of the base plate (1), the two fixed plates (2) are arranged in bilateral symmetry about the central axis of the base plate (1), grooves (3) are formed in one side, close to each other, of each fixed plate (2), an adjusting mechanism and a limiting mechanism are respectively arranged in each groove (3), the adjusting mechanism is positioned on one side of the limiting mechanism, two movable plates (4) are arranged between the two fixed plates (2), an outer ring plate (5) is fixedly arranged between the two movable plates (4), an inner ring plate (6) is arranged in each outer ring plate (5), the outer wall of each inner ring plate (6) is movably connected with the inner wall of each outer ring plate (5) through a bearing, a test tube (7) is arranged in each inner ring plate (6), a sealing plug (8) is arranged in the top end of each test tube (7), and a clamping mechanism is fixedly arranged on the rear side of the top end of each inner ring plate (6), screw thread channel (9) have been seted up to interior crown plate (6) outer wall, screw thread channel (9) bottom inside is equipped with reference column (10), reference column (10) rear side is equipped with backup pad (11), backup pad (11) bottom and base plate (1) top fixed connection, backup pad (11) top front side and reference column (10) rear end fixed connection.
2. The experimental device for cell precipitation in research on osteogenic differentiation mechanism of forced spondylitis of claim 1, wherein: the adjusting mechanism comprises a first threaded rod (12), the first threaded rod (12) is located inside a groove (3) in one of the fixing plates (2), the top end and the bottom end of the first threaded rod (12) are respectively movably connected with the top and the bottom of an inner cavity of the groove (3) through bearings, a movable plate (4) located on one side, close to the first threaded rod (12), of the outer annular plate (5) is sleeved outside the first threaded rod (12), and the movable plate (4) is in threaded connection with the first threaded rod (12).
3. The experimental device for cell precipitation in research on osteogenic differentiation mechanism of forced spondylitis of claim 1, wherein: the sections of the movable plate (4) and the groove (3) are both rectangular.
4. The experimental device for cell precipitation in research on osteogenic differentiation mechanism of forced spondylitis of claim 1, wherein: the limiting mechanism comprises a guide rod (13), the guide rod (13) is located inside a groove (3) in one of the fixing plates (2), the top end and the bottom end of the guide rod (13) are fixedly connected with the top and the bottom of an inner cavity of the groove (3) respectively, and a movable plate (4) located on one side, close to the guide rod (13), of the outer ring plate (5) is sleeved outside the guide rod (13).
5. The experimental device for cell precipitation in research on osteogenic differentiation mechanism of forced spondylitis of claim 2, wherein: and the top of the fixing plate (2) positioned outside the first threaded rod (12) is fixedly provided with a miniature positive and negative motor (14), and the output end of the miniature positive and negative motor (14) penetrates through the fixing plate (2) and is fixedly connected with the top end of the first threaded rod (12).
6. The experimental device for cell precipitation in research on osteogenic differentiation mechanism of forced spondylitis of claim 1, wherein: fixture includes diaphragm (15), diaphragm (15) are located interior crown plate (6) top rear side, diaphragm (15) front side is fixed and is equipped with connecting plate (16), connecting plate (16) and interior crown plate (6) top rear side fixed connection, spacing groove (17) have been seted up at diaphragm (15) top, inside second threaded rod (18) that is equipped with of spacing groove (17), second threaded rod (18) both ends all through the bearing respectively with spacing groove (17) both sides inner wall swing joint, the equal threaded connection in second threaded rod (18) both ends outer wall has slide (19).
7. The experimental device for cell precipitation in research on osteogenic differentiation mechanism for ankylosing spondylitis according to claim 6, wherein: the screw thread direction of two ends of the second threaded rod (18) is opposite, the threads of two ends of the second threaded rod (18) are symmetrically arranged about the central axis of the second threaded rod (18), and the cross sections of the sliding seat (19) and the limiting groove (17) are both set to be rectangular.
8. The experimental device for cell precipitation in research on osteogenic differentiation mechanism for ankylosing spondylitis according to claim 6, wherein: test tube (7) top both sides all are equipped with grip block (20), grip block (20) contact with test tube (7) one side, grip block (20) rear end and slide (19) top front side fixed connection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122053793.1U CN215727178U (en) | 2021-08-27 | 2021-08-27 | Cell precipitation experimental device for research of forced spondylitis osteogenesis differentiation mechanism |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122053793.1U CN215727178U (en) | 2021-08-27 | 2021-08-27 | Cell precipitation experimental device for research of forced spondylitis osteogenesis differentiation mechanism |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215727178U true CN215727178U (en) | 2022-02-01 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202122053793.1U Expired - Fee Related CN215727178U (en) | 2021-08-27 | 2021-08-27 | Cell precipitation experimental device for research of forced spondylitis osteogenesis differentiation mechanism |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215727178U (en) |
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2021
- 2021-08-27 CN CN202122053793.1U patent/CN215727178U/en not_active Expired - Fee Related
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Granted publication date: 20220201 |