CN215677761U

CN215677761U - Synthesize device that formula separation extracted soil nematode

Info

Publication number: CN215677761U
Application number: CN202121118646.1U
Authority: CN
Inventors: 黄菊; 陈浒; 肖聶佳; 魏强; 周园园; 殷秀梅; 刘文俊; 周文君
Original assignee: Guizhou Education University
Current assignee: Guizhou Education University
Priority date: 2021-05-24
Filing date: 2021-05-24
Publication date: 2022-01-28
Anticipated expiration: 2031-05-24

Abstract

The utility model discloses a device for comprehensively separating and extracting soil nematodes, belonging to the technical field of biological collection research devices; it comprises a vertical column; a top plate is arranged at the upper top of the upright post; a plurality of funnels are arranged on the top plate; the liquid outlet of the funnel is arranged at the lower part of the top plate, and a deformable rubber tube is also arranged at the position of the liquid outlet of the funnel; auxiliary support plates are respectively arranged in the middle of the upright post and at positions close to the top plate; the rubber tube sequentially penetrates through the two auxiliary supporting plates, and a water stop valve is arranged at the position where the two auxiliary supporting plates are connected with the rubber tube; the bottom of the upright post is also provided with a separating disc; and a mesh screen is arranged in the separating disc; the utility model effectively solves the problem that the prior nematode extracting and separating device which can integrate the advantages of the berman funnel method and the Kabo sieving method is lacked.

Description

Synthesize device that formula separation extracted soil nematode

Technical Field

The utility model relates to the technical field of biological collection research devices, in particular to a comprehensive device for separating and extracting soil nematodes.

Background

When the soil nematodes are researched, the soil nematodes are mainly separated and extracted by a Beeman funnel method and a Calbee sieving method at present. Both separation methods have respective advantages and disadvantages, such as that the method of Beeman funnel can separate active worm-type nematodes, but the separated substances have more soil residues and other substances; the Calbo sieving method can treat soil samples in large batches for extraction, but the isolate contains more necrotizing nematodes, and how to overcome the respective defects while integrating the advantages of the two extraction methods is the main problem faced at present.

SUMMERY OF THE UTILITY MODEL

The technical problem to be solved by the utility model is as follows: provides a device for comprehensively separating and extracting soil nematodes, which solves the problem that a nematode extracting and separating device which can integrate the advantages of a Bermann funnel method and a Calbee sieving method is not available at present.

In order to solve the problems, the utility model provides the following technical scheme:

a device for comprehensively separating and extracting soil nematodes; it comprises a vertical column; a top plate is arranged at the upper top of the upright post; a plurality of funnels are arranged on the top plate; the liquid outlet of the funnel is arranged at the lower part of the top plate, and a deformable rubber tube is also arranged at the position of the liquid outlet of the funnel; auxiliary support plates are respectively arranged in the middle of the upright post and at positions close to the top plate; the rubber tube sequentially penetrates through the two auxiliary supporting plates, and a water stop valve is arranged at the position where the two auxiliary supporting plates are connected with the rubber tube; the bottom of the upright post is also provided with a separating disc; and a mesh screen is provided in the separating discs.

Preferably, the top plate and the auxiliary support plate are both disc-shaped structures; the upright post is arranged in the middle of the two; the funnels are placed evenly with the center line of the top plate as the center of symmetry.

Preferably, the rubber tube is also provided with a pressure regulating branch tube communicated with the inner cavity of the rubber tube; a plug rod in a cantilever structure is outwards arranged at the side part of the upright post; the rod diameter of the plug rod is matched with the pipe diameter of the pressure regulating branch pipe.

Preferably, the water stop valve is of a manual mechanical valve structure, and the water stop valve is arranged on the upper sides of the two auxiliary supporting plates.

Preferably, a guide convex strip is arranged on the periphery of the top of the separating disc; the movable table is movably arranged on the separating disc through the guide convex strip; a pipe sleeve is also arranged on the movable table; the flushing pipe is detachably mounted on the movable table through the pipe sleeve.

Preferably, the middle part of the bottom in the separating disc is also provided with a mounting cylinder; the upright column is detachably connected with the separating disc through the mounting cylinder.

Preferably, a drainage pipe communicated with the inner cavity of the separating disc is further arranged on the side wall of the separating disc close to the bottom.

The utility model has the beneficial effects that:

the utility model designs a comprehensive device for separating nematodes from soil, which integrates the instruments needed by a Berman funnel method and a Calbee sieving method, takes an upright post as a main support of the whole device, further arranges a top plate for placing a funnel, arranges an auxiliary support plate for a rubber tube to pass through on the upright post, and realizes the control of the nematodes in the funnel and the amount of filtrate leaked downwards by using a water stop valve arranged on the auxiliary support plate; in addition, a separation disc is arranged at the bottom of the upright column, so that a large amount of nematodes, impurities and filtrate flowing out of the rubber hoses enter the mesh screen, and the separation disc is washed to obtain filtered and washed nematodes which can be poured into a specimen bottle for collection.

Drawings

FIG. 1 is a schematic structural view of the present invention in an embodiment;

FIG. 2 is a top view of FIG. 1;

description of reference numerals: 1. the device comprises a stand column, 2, a top plate, 3, a funnel, 4, a rubber tube, 5, an auxiliary support plate, 6, a water stop valve, 7, a separating disc, 8, a mesh screen, 9, a movable table, 10, a pipe sleeve, 11, an installation cylinder, 12, a plug rod, 13 and a pressure regulating branch pipe.

Detailed Description

The utility model will be further described with reference to the following drawings and specific embodiments:

example (b):

referring to fig. 1, the present embodiment provides a comprehensive device for separating and extracting soil nematodes; it comprises a vertical column 1; a top plate 2 is arranged at the upper top of the upright post 1; a plurality of funnels 3 are arranged on the top plate 2; a liquid outlet of the funnel 3 is arranged at the lower part of the top plate 2, and a deformable rubber tube 4 is also arranged at the position of the liquid outlet of the funnel 3; auxiliary support plates 5 are respectively arranged at the middle part of the upright post 1 and the positions close to the top plate 2; the rubber tube 4 sequentially penetrates through the two auxiliary supporting plates 5, and a water stop valve 6 is further arranged at the position where the two auxiliary supporting plates 5 are connected with the rubber tube 4; the bottom of the upright post 1 is also provided with a separating disc 7; and a mesh screen 8 is provided in the separating disc 7. The hose used in this example is a transparent tube made of plastic, and the mesh screen 8 uses a standard 400 mesh screen.

The top plate 2 and the auxiliary support plate 3 are both disc-shaped structures; the upright post 1 is arranged at the middle position of the upright post and the upright post; the funnels 3 are uniformly placed with the center line of the top plate 1 as the center of symmetry.

The rubber tube 4 is also provided with a pressure regulating branch pipe 13 communicated with the inner cavity of the rubber tube; a plug rod 12 in a cantilever structure is arranged outwards at the side part of the upright post 1; the rod diameter of the plug rod 12 is matched with the pipe diameter of the pressure regulating branch pipe 13. The purpose of arranging the pressure regulating branch pipe 13 is to ensure that the air pressure at two sides from the upper section water stop valve of the rubber pipe to the lower outlet position is constant, so that water in the rubber pipe can flow downwards; the plug rod 12 is used for sealing the pressure regulating branch pipe 13 when water in the rubber pipe is not drained;

the water stop valves 6 are of a manual mechanical valve structure, and the water stop valves 6 are arranged on the upper sides of the two auxiliary supporting plates 5. The water stop valve 6 in this embodiment is a rotary disc structure, and the manual control structure is convenient for an operator to operate the water stop valve.

Guiding convex strips are arranged on the periphery of the top of the separating disc 7; the movable table 9 is movably mounted on the separating tray 7 through the guide convex strip; a pipe sleeve 10 is also arranged on the movable table 9; the flushing pipe is detachably mounted on the movable table 9 via this pipe sleeve 10. The cooperation of the guiding convex strip and the movable table 9 can refer to fig. 1, wherein the guiding convex plate is an annular structure with a T-shaped section, a T-shaped groove with a size corresponding to the size is arranged at the bottom of the movable table 9, and the guiding convex strip and the movable table are movably arranged on the guiding convex strip through the cooperation of the guiding convex strip and the movable table; the movable table 9 capable of moving is provided with the flushing pipe, so that the flushing pipe can flush nematodes and other impurities on the net sieve in the separation disc 7 uniformly at different angles.

The middle part of the bottom in the separating disc 7 is also provided with a mounting cylinder 11; the column 1 is detachably connected to the separating discs 7 via the mounting tube 11. The mounting cylinder 11 and the separating discs 7 are of concentric thin-walled tube columnar structures, the mounting of the separating discs 7 and the stand columns can be completed by placing the stand columns 1 on the mounting cylinder 11, it needs to be noted that the middle parts of the mesh screens 8 arranged on the separating discs are wound on the outer side wall of the mounting cylinder 11, and meanwhile, sealing rings are further arranged at the connecting positions of the stand columns 1 on the mounting cylinder 11 so as to place water splashed into the mounting cylinder during washing.

The side wall of the separating disc 7 close to the bottom is also provided with a discharge pipe communicated with the inner cavity of the separating disc.

When the device is used, firstly, a batch of soil samples prepared in advance are respectively put into the gauze, the wire gauze used as a support is placed in the funnel, then, the soil wrapped by the gauze is respectively put into the funnel, and then, water is added into the funnel; simultaneously, opening a water stop valve on the auxiliary support plate; an operator needs to observe the condition of the mixed liquid seeped in the rubber tube at the moment, and closes the upper and lower water stop valves after the rubber tube is filled with the mixed liquid, and meanwhile, the plug rod is in butt joint with the pressure regulating branch pipe; after the device is kept stand for 48 hours, the water stop valves on the auxiliary support plates at the lower parts of the water stop valves are opened respectively, the pressure regulating branch pipes are separated from the plug rods, the pressure on the side of each pressure regulating branch pipe is balanced with the pressure on the bottom of the rubber pipe, and at the moment, the mixed liquid in the rubber pipe moves downwards to enter the screen of the separating disc. Then, a washing pipe and a water source which are arranged in the pipe sleeve 10 are communicated, so that washing water can enter the separation disc to wash the mixed liquid on the mesh screen, the washing pipe to be rotated can be matched with the movable table through the guide raised lines in the washing process, the mixed liquid can be washed in all directions until the nematodes are completely separated from other magazines, and in the process, the washing water overflows from a drainage pipe at the bottom of the separation disc; and finally, the vertical column and the separation disc are disassembled, and the nematodes are transferred to the specimen bottle for storage, so that the whole operation process is completed.

Claims

1. The utility model provides a device of soil nematode is drawed in comprehensive formula separation which characterized in that: it comprises a vertical column (1); a top plate (2) is arranged at the upper top of the upright post (1); a plurality of funnels (3) are arranged on the top plate (2); a liquid outlet of the funnel (3) is arranged at the lower part of the top plate (2), and a deformable rubber tube (4) is also arranged at the position of the liquid outlet of the funnel (3); auxiliary support plates (5) are respectively arranged at the middle part of the upright post (1) and the position close to the top plate (2); the rubber tube (4) sequentially penetrates through the two auxiliary support plates (5), and a water stop valve (6) is arranged at the position where the two auxiliary support plates (5) are connected with the rubber tube (4); the bottom of the upright post (1) is also provided with a separating disc (7); and a mesh screen (8) is arranged in the separating disc (7).

2. The device for comprehensively separating and extracting the soil nematodes according to claim 1, wherein: the top plate (2) and the auxiliary support plate are both disc-shaped structures; the upright post (1) is arranged in the middle of the upright post and the upright post; the funnels (3) are uniformly placed with the center line of the top plate as the center of symmetry.

3. The device for comprehensively separating and extracting the soil nematodes according to claim 1, wherein: the rubber tube (4) is also provided with a pressure regulating branch tube (13) communicated with the inner cavity of the rubber tube; a plug rod (12) in a cantilever structure is outwards arranged at the side part of the upright post (1); the rod diameter of the plug rod (12) is matched with the pipe diameter of the pressure regulating branch pipe (13).

4. The device for comprehensively separating and extracting the soil nematodes according to claim 1, wherein: the water stop valves (6) are of manual mechanical valve structures, and the water stop valves (6) are arranged on the upper sides of the two auxiliary supporting plates (5).

5. The device for comprehensively separating and extracting the soil nematodes according to claim 1, wherein: the periphery of the top of the separating disc (7) is also provided with a guide convex strip; the movable table (9) is movably arranged on the separating disc (7) through the guide convex strip; a pipe sleeve (10) is also arranged on the movable table (9); the flushing pipe is detachably mounted on the movable table (9) through the pipe sleeve (10).

6. The device for comprehensively separating and extracting the soil nematodes according to claim 1, wherein: the middle part of the bottom in the separating disc (7) is also provided with an installation cylinder (11); the column (1) is detachably connected to the separating disk (7) via the mounting cylinder (11).

7. The device for comprehensively separating and extracting the soil nematodes according to claim 1, wherein: the side wall of the separating disc (7) close to the bottom is also provided with a discharge pipe communicated with the inner cavity of the separating disc.