CN215667786U

CN215667786U - Protein renaturation device

Info

Publication number: CN215667786U
Application number: CN202122218941.0U
Authority: CN
Inventors: 单玉飞; 张开岳; 左胜涵
Original assignee: Zhenjiang Ruihua Biotechnology Co ltd
Current assignee: Zhenjiang Ruihua Biotechnology Co ltd
Priority date: 2021-09-14
Filing date: 2021-09-14
Publication date: 2022-01-28
Anticipated expiration: 2031-09-14

Abstract

The utility model provides a protein renaturation device which comprises a first container, wherein a motor is installed at the top of the first container, a liquid separation plate which is arranged in the first container and used for dispersing liquid is installed on an output shaft of the motor, a supporting plate is installed on the lower side of the inner wall of the first container, a second container is arranged at the top of the supporting plate, a liquid inlet pipe which sequentially penetrates through the bottom of the first container, the supporting plate and the bottom of the second container is installed at the center of the bottom of the liquid separation plate, a plurality of flow guide pipes which are arranged in the second container are symmetrically arranged at the bottom of the liquid separation plate, a water pump is installed on the liquid inlet pipe, a sealing bearing is installed at the joint of the liquid inlet pipe and the liquid separation plate, and a plurality of bases are arranged at the bottom of the first container.

Description

Protein renaturation device

Technical Field

The utility model relates to a protein renaturation device, belonging to the technical field of protein renaturation devices.

Background

Protein renaturation means that when the denaturation condition is not severe and the internal structure of the denatured protein is not greatly changed, the denaturation factor is removed, the protein can restore the natural conformation and the biological activity under proper conditions, and a protein renaturation device is needed when obtaining the renaturated protein.

In the prior art, when the protein renaturation device is used, liquid is generally directly introduced into a container provided with a protein dialysis bag to be renatured to reduce the concentration of dialysate, the impact force of the liquid introduced into the container is large, and the liquid is not uniformly dispersed, so that the progress of reducing the concentration of the dialysate is reduced, and the protein renaturation efficiency is low; and is inconvenient for stirring the solution, has poor using effect, and urgently needs a protein renaturation device to solve the problems.

SUMMERY OF THE UTILITY MODEL

Aiming at the defects in the prior art, the utility model aims to provide a protein renaturation device to solve the problems in the background technology.

In order to achieve the purpose, the utility model is realized by the following technical scheme: the utility model provides a protein renaturation device, includes first container, the motor is installed at first container top, install on the output shaft of motor and arrange the first container inside branch liquid board that is used for carrying out the dispersion to liquid in, the backup pad is installed to first container inner wall downside, the backup pad top is provided with the second container, divide liquid board bottom central point department of putting to install the feed liquor pipe that runs through first container bottom, backup pad and second container bottom in proper order, divide liquid board bottom symmetry to be provided with a plurality of honeycomb ducts of arranging the second container inside in, install the water pump on the feed liquor pipe, seal bearing is installed with branch liquid board junction to the feed liquor pipe, first container bottom is provided with a plurality of bases.

Further, a liquid outlet pipe is arranged on the side wall of the first container.

Further, first container lateral wall is installed and is used for carrying out the transparent observation window that observes to the inside solution of second container, first container lateral wall is installed and is used for the slope inlet pipe to second container transported substance material.

Furthermore, a throttle valve is installed on the liquid inlet pipe.

Furthermore, a sealing gasket sleeved on the liquid inlet pipe is installed at the joint of the second container and the supporting plate.

Further, the top of the second container is of an open structure.

Furthermore, the liquid inlet pipe is communicated with the liquid separation plate, and the flow guide pipe is communicated with the liquid separation plate.

Further, a sealing door is arranged on the side wall of the first container through a rotating hinge.

The utility model has the beneficial effects that: when the protein renaturation device is used:

1. the liquid entering the liquid separation plate is conveniently dispersed by using the plurality of guide pipes, the impact force of the liquid flowing into the second container filled with the protein dialysis bag to be renatured is reduced, so that the progress of reducing the concentration of the dialysate is accelerated, the renaturation efficiency of the protein is improved, the liquid flowing into the second container is uniformly dispersed by symmetrically arranging the plurality of guide pipes, the concentration of the dialysate is uniformly reduced by the liquid, and the renaturation efficiency of the protein is secondarily improved;

2. through when improving protein renaturation efficiency, use a plurality of honeycomb ducts to stir liquid, play the effect of being convenient for stir liquid.

Drawings

Other features, objects and advantages of the utility model will become more apparent upon reading of the detailed description of non-limiting embodiments with reference to the following drawings:

FIG. 1 is a perspective view of a protein renaturation device according to the present invention;

FIG. 2 is a front cross-sectional view of a protein renaturation device according to the present invention;

FIG. 3 is an enlarged view of A of FIG. 2 according to the present invention;

FIG. 4 is an enlarged view of B of FIG. 2 according to the present invention;

in the figure: 1-motor, 2-first container, 201-transparent observation window, 202-inclined feeding pipe, 3-liquid outlet pipe, 4-liquid inlet pipe, 41-throttle valve, 5-water pump, 6-base, 7-supporting plate, 8-second container, 9-flow guide pipe, 10-liquid separation plate, 11-sealing bearing and 12-sealing gasket.

Detailed Description

In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the utility model easy to understand, the utility model is further described with the specific embodiments.

Referring to fig. 1-4, the present invention provides a technical solution: a protein renaturation device comprises a first container 2, a motor 1 is installed at the top of the first container 2, a liquid separation plate 10 which is arranged inside the first container 2 and used for dispersing liquid is installed on an output shaft of the motor 1, a supporting plate 7 is installed on the lower side of the inner wall of the first container 2, a second container 8 is arranged at the top of the supporting plate 7, a liquid inlet pipe 4 which sequentially penetrates through the bottom of the first container 2, the supporting plate 7 and the bottom of the second container 8 is installed at the center of the bottom of the liquid separation plate 10, a plurality of flow guide pipes 9 which are arranged inside the second container 8 are symmetrically arranged at the bottom of the liquid separation plate 10, a water pump 5 is installed on the liquid inlet pipe 4, a sealing bearing 11 is installed at the joint of the liquid inlet pipe 4 and the liquid separation plate 10, a plurality of bases 6 are arranged at the bottom of the first container 2, the design facilitates the dispersion of the liquid entering the liquid separation plate 10 by using the plurality of flow guide pipes 9, the impact force of the liquid flowing into the inside of the second container 8 containing a protein dialysis bag to be renatured is reduced, thereby accelerate the progress that reduces the dislysate concentration, improve protein renaturation efficiency, be provided with a plurality of honeycomb ducts 9 through the symmetry and make the liquid homodisperse that flows into 8 insides of second container, make the even dislysate concentration that reduces of liquid, the secondary improves protein renaturation efficiency, and when improving protein renaturation efficiency, uses a plurality of honeycomb ducts 9 to stir liquid, plays the effect of being convenient for stir liquid.

2 lateral walls of first container are provided with drain pipe 3, this design is through using drain pipe 3, be convenient for discharge the inside liquid of

first container

2, 2 lateral walls of first container install be used for carrying out the transparent observation window 201 that observes to the inside solution of

second container

8, 2 lateral walls of first container install the slope inlet pipe 202 that is used for carrying the material to second container 8, this design is through using transparent observation window 201, be convenient for observe the inside solution of second container 8, be convenient for send the material to inside the second container 8 through using slope inlet pipe 202, the material is for having been equipped with and treating renaturation protein dialysis bag, install choke valve 41 on the feed liquor pipe 4, this design is convenient for adjust the liquid flow rate that gets into feed liquor pipe 4 inside through using choke valve 41, thereby reduce the impact force that liquid flows into and divides liquid board 10.

Second container

8 and 7 junctions of backup pad are installed the cover and are established sealed 12 of filling in the pad on the liquid pipe 4, this design is through using sealed 12 of filling in, the leakproofness of junction has been improved, 8 tops of second container are opening column structure, the rationality of this design has been improved, liquid pipe 4 is linked together with branch liquid board 10, honeycomb duct 9 is linked together with branch liquid board 10, this design is convenient for liquid and is flowed into branch liquid board 10 inside along liquid pipe 4, be convenient for liquid and flow out from honeycomb duct 9, be provided with sealing door through rotating the hinge on the 2 lateral walls of first container.

As an embodiment of the present invention: when the renatured protein needs to be obtained, a worker firstly sends a material filled with a dialysis bag for renatured protein into the second container 8 from the inclined feeding pipe 202, then the water pump 5 passes through the material, the external liquid is sent into the liquid distribution plate 10 along the feeding pipe, then the material flows out of the plurality of flow guide pipes 9 and enters the second container 8, meanwhile, the motor 1 works and rotates to drive the flow guide pipes 9 on the liquid distribution plate 10 to rotate to stir the liquid, the liquid is added into the second container 8 to continuously reduce the concentration of the liquid, the overflowed liquid enters the first container 2 from the second container 8, and finally the protein flows out of the liquid outlet pipe 3, so that the renatured protein can be obtained.

Through using a plurality of honeycomb ducts 9, be convenient for disperse the liquid that gets into the inside of minute liquid board 10, reduce the liquid and flow into the inside impact force of second container 8 that has been equipped with the protein dialysis bag of waiting to renaturate, thereby accelerate the progress that reduces the dislysate concentration, improve protein renaturation efficiency, through being provided with a plurality of honeycomb ducts 9 of symmetry and making the liquid that flows into the inside of second container 8 evenly disperse, make liquid evenly reduce the dislysate concentration, the secondary improves protein renaturation efficiency, and when improving protein renaturation efficiency, use a plurality of honeycomb ducts 9 to stir liquid, play the effect of being convenient for stir liquid, improve the practicality of the utility model.

While there have been shown and described what are at present considered the fundamental principles and essential features of the utility model and its advantages, it will be apparent to those skilled in the art that the utility model is not limited to the details of the foregoing exemplary embodiments, but is capable of other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the utility model being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims

1. A protein renaturation device characterized in that: comprises a first container (2), a motor (1) is arranged at the top of the first container (2), a liquid separation plate (10) which is arranged in the first container (2) and used for dispersing liquid is arranged on an output shaft of the motor (1), a supporting plate (7) is arranged on the lower side of the inner wall of the first container (2), a second container (8) is arranged on the top of the supporting plate (7), a liquid inlet pipe (4) which sequentially penetrates through the bottom of the first container (2), the supporting plate (7) and the bottom of the second container (8) is arranged at the center of the bottom of the liquid separating plate (10), a plurality of draft tubes (9) arranged in the second container (8) are symmetrically arranged at the bottom of the liquid separating plate (10), install water pump (5) on feed liquor pipe (4), sealed bearing (11) are installed to feed liquor pipe (4) and branch liquid board (10) junction, first container (2) bottom is provided with a plurality of bases (6).

2. The protein renaturation device according to the claim 1 characterized in that: the side wall of the first container (2) is provided with a liquid outlet pipe (3).

3. The protein renaturation device according to the claim 1 characterized in that: transparent observation window (201) that is used for observing the inside solution of second container (8) is installed to first container (2) lateral wall, first container (2) lateral wall is installed and is used for feeding inclined inlet pipe (202) of material to second container (8).

4. The protein renaturation device according to the claim 1 characterized in that: and a throttle valve (41) is arranged on the liquid inlet pipe (4).

5. The protein renaturation device according to the claim 1 characterized in that: and a sealing gasket (12) sleeved on the liquid inlet pipe (4) is arranged at the joint of the second container (8) and the supporting plate (7).

6. The protein renaturation device according to the claim 1 characterized in that: the top of the second container (8) is of an open structure.

7. The protein renaturation device according to the claim 1 characterized in that: the liquid inlet pipe (4) is communicated with the liquid separation plate (10), and the flow guide pipe (9) is communicated with the liquid separation plate (10).

8. The protein renaturation device according to the claim 1 characterized in that: and a sealing door is arranged on the side wall of the first container (2) through a rotating hinge.