CN215655768U - Centrifugal machine for nucleic acid amplification - Google Patents
Centrifugal machine for nucleic acid amplification Download PDFInfo
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- CN215655768U CN215655768U CN202122154407.8U CN202122154407U CN215655768U CN 215655768 U CN215655768 U CN 215655768U CN 202122154407 U CN202122154407 U CN 202122154407U CN 215655768 U CN215655768 U CN 215655768U
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- nucleic acid
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- acid amplification
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Abstract
The utility model relates to the technical field of biological detection, and discloses a centrifugal machine for nucleic acid amplification, which comprises a shell, and an incubation bin and a centrifugal bin which are arranged in the shell, wherein a centrifugal device is arranged in the centrifugal bin, the centrifugal device comprises a rotating shaft which is vertically and rotatably arranged in the centrifugal bin, and a rotor which is detachably arranged on the rotating shaft and synchronously rotates with the rotating shaft, at least one rotor is arranged, the rotor comprises a rotor body, and a first accommodating part and/or a second accommodating part, which are arranged on the rotor body and used for inserting a tubular container, and/or a plate-shaped container, at least one first accommodating part and at least one second accommodating part are respectively arranged, the centrifugal machine can realize low-speed throwing-off of small tubular containers, sheet containers and disc-shaped containers, and high-speed centrifugation of large tubular containers, and the applicability is improved.
Description
Technical Field
The utility model relates to the technical field of biological detection, in particular to a centrifugal machine for nucleic acid amplification.
Background
Nucleic acid detection needs to be carried out by extracting nucleic acid in a sample firstly and then carrying out isothermal amplification, a common laboratory puts a container filled with the sample and lysis solution into a centrifuge, realizes sample lysis and nucleic acid purification by adjusting the rotating speed of a centrifugal device, puts the purified nucleic acid into an isothermal amplification instrument for nucleic acid amplification, but the isothermal amplification container and the centrifuge are often placed separately, the vibration amplitude of the container transferred from the centrifuge to the isothermal amplification instrument is large on the way, the purified nucleic acid is possibly mixed with waste liquid to cause pollution, and therefore, a device which integrates the isothermal amplification instrument and the centrifuge is provided.
Chinese patent with application number CN201821303019.3 discloses a constant temperature blood type centrifuge, including the device body, install at the closing cap at device body top and in hatching storehouse and the centrifugation storehouse that the inside of device body set up respectively, the inside in centrifugation storehouse is provided with centrifuge, centrifuge includes centrifugal disc, and centrifugal disc's surface is provided with a plurality of test tube clamp area, centrifugal disc's bottom is provided with the work motor.
The centrifugal disc of the centrifuge is internally provided with a test tube clamp belt for accommodating tubular containers such as test tubes, centrifuge tubes and the like, but because the containers used in biological detection research are different, various plate-shaped containers are more appeared in recent years, including disc-shaped containers such as microfluidic disc chips and sheet-shaped containers such as microarray chips, so that the centrifugal disc is only provided with an accommodating groove for accommodating the tubular containers, and the requirements of centrifuging the containers with various shapes and specifications cannot be met.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a centrifugal machine for nucleic acid amplification, which can be used for centrifuging containers with various shapes and specifications and improving the applicability.
In order to achieve the purpose, the utility model provides the following technical scheme: the utility model provides a centrifuge for nucleic acid amplification, including the casing and in the inside hatching storehouse and the centrifugation storehouse that set up of casing, be provided with centrifugal device in the centrifugation storehouse, centrifugal device includes the pivot that sets up in the vertical rotation of centrifugation storehouse and can dismantle the rotor that sets up and pivot synchronous revolution in the pivot, the rotor is provided with at least one, the rotor includes rotor body and the confession tubular container male first holding portion and/or confession platelike container male second holding portion that sets up on rotor body, first holding portion and second holding portion are provided with at least one respectively.
By adopting the scheme, compared with the centrifugal disc in the prior art which is only provided with the containing groove for placing the tubular container, but because the containers used in the biological experiment research are different, various plate-shaped containers are more appeared in recent years, including disc-shaped containers such as microfluidic disc chips and sheet-shaped containers such as microarray chips, and the circular groove for placing the centrifugal tube on the rotor can not meet the requirement of centrifuging the containers with various shapes and specifications, the scheme adds the second containing part on the rotor body for placing the plate-shaped containers, and because the rotor body is provided with the first containing part for placing the tubular containers, the tubular containers and the plate-shaped containers can be placed in the centrifuge for centrifugation, the applicability is improved, the first containing part and the second containing part can be arranged on the same or different rotor bodies, and the same-batch centrifugation quantity of the same containers can be increased on different rotor bodies, the efficiency is improved, the replacement frequency can be reduced when the rotor bodies are arranged on the same rotor body, and the service life is prolonged.
Furthermore, the first holding portion comprises a plurality of circular grooves formed in the inner side wall of the rotor body, and the inner diameters of the circular grooves symmetrically formed along the axial direction of the rotating shaft are equal.
By adopting the scheme, the tubular container is inserted into the circular groove and rotates along with the rotor body for centrifugation, the inner diameter of the circular groove which is symmetrically arranged along the axial direction of the rotating shaft is equal, the balance weight on the rotor body after the tubular container is placed is ensured to be equal, the violent shaking of the centrifuge when the centrifuge rotates at a high speed due to the unbalance of the balance weight is avoided, and the safety during use is improved.
Furthermore, at least two circular grooves with different inner diameters are arranged on the inner side wall of the rotor body.
By adopting the scheme, the circular grooves with two or more different inner diameters are arranged, so that the rotor body can accommodate tubular containers with more specifications, the applicability of the rotor is improved, and the replacement frequency of the rotor is reduced.
Furthermore, the second accommodating part comprises accommodating grooves which are symmetrically arranged on the inner side wall of the rotor body along the axial direction of the rotating shaft and are used for inserting the plate-shaped containers.
By adopting the scheme, the plate-shaped container is inserted into the accommodating groove and rotates along with the rotor body for centrifugation, the accommodating groove is axially and symmetrically arranged along the rotating shaft to ensure that the balance weight on the rotor body after the plate-shaped container is placed is equal, severe shaking of the centrifuge when the centrifuge rotates at a high speed due to unbalance of the balance weight is avoided, and the safety during use is improved.
Furthermore, insertion grooves are symmetrically formed in the inner side wall of the rotor body along the axial direction of the rotating shaft, an installation block is detachably arranged in each insertion groove, and the accommodating grooves are formed in the installation blocks.
By adopting the scheme, the mounting block can be detachably arranged in the insertion groove, the plate-shaped container can be inserted into or taken from the rotor body only by plugging the mounting block, the plate-shaped container can be taken out of or placed in the centrifuge, the taking and the replacement of the plate-shaped container can be carried out outside the centrifuge, the time is saved, and the efficiency is improved.
Furthermore, the second accommodating portion comprises mounting plates symmetrically arranged on the outer wall of the end, far away from the rotating shaft, of the rotor body, baffles arranged in the direction far away from the rotor body at the end, far away from the rotating shaft, of each mounting plate, and an accommodating space formed between the two baffles for the plate-shaped container to be inserted in along the axial direction of the rotating shaft.
By adopting the scheme, the baffle plates which are symmetrically arranged form the accommodating space, the plate-shaped container is inserted into the accommodating space and is in guide fit with the baffle plates, the rotor body rotates to drive the mounting plate to rotate, and the baffle plates synchronously rotate along with the mounting plate to drive the plate-shaped container to rotate for centrifugation.
Furthermore, linkage structures for driving the plate-shaped container and the rotor body to synchronously rotate are respectively arranged between the two ends of the plate-shaped container and the baffle plates.
By adopting the scheme, because the surfaces of the baffle and the plate-shaped container are smooth, the baffle cannot drive the plate-shaped container to rotate when rotating, a linkage structure is additionally arranged between the baffle and the plate-shaped container to drive the plate-shaped container to rotate synchronously along with the baffle,
furthermore, the linkage structure comprises a guide groove arranged on the surface of the plate-shaped container close to the baffle and a convex block convexly arranged on the surface of the baffle close to the plate-shaped container and matched with the guide groove in a guide sliding way.
By adopting the scheme, the lug and the guide groove are clamped to limit the relative rotation between the baffle and the plate-shaped container, so that the plate-shaped container is ensured to rotate synchronously along with the baffle, and the lug and the guide groove are guided to slide and matched to avoid the plate-shaped container from being shifted when being inserted into the accommodating space.
Furthermore, one side of the baffle close to the plate-shaped container is in a plane shape or in an arc shape concentric with the rotating shaft.
Adopt above-mentioned scheme, set up to the planar more suitable slice container use, set up to the circular-arc more suitable discoid container use of concentric with the pivot.
Furthermore, a support plate is arranged on the inner side wall of the rotor body, and the upper end surface of the support plate is abutted against the bottom surface of the plate-shaped container when the plate-shaped container is inserted.
By adopting the scheme, the support plate can improve the stability of the plate-shaped container when being placed.
Compared with the prior art, the utility model has the following beneficial effects: the second containing part is additionally arranged on the rotor body to place the plate-shaped container, and the first containing part is arranged on the rotor body to place the tube-shaped container, so that the tube-shaped container and the plate-shaped container can be placed in the centrifuge for centrifugation, the applicability is improved, the first containing part and the second containing part can be arranged on the same or different rotor bodies, the same-batch centrifugation quantity of the same container can be increased on different rotor bodies, the efficiency is improved, the replacement frequency can be reduced on the same rotor body, and the service life is prolonged.
Drawings
The accompanying drawings, which are included to provide a further understanding of the utility model and are incorporated in and constitute a part of this specification, illustrate embodiments of the utility model and together with the description serve to explain the principles of the utility model and not to limit the utility model. In the drawings:
FIG. 1 is an isometric view of a centrifuge for nucleic acid amplification according to the present invention;
FIG. 2 is an exploded view of a centrifuge for nucleic acid amplification according to the present invention when the centrifuge is installed in a first rotor body;
FIG. 3 is an isometric view of a first rotor body in a centrifuge for nucleic acid amplification of the present invention;
FIG. 4 is an isometric view of a second rotor body in a centrifuge for nucleic acid amplification of the present invention.
In the figure: 1. a housing; 101. a centrifugal bin; 2. a rotating shaft; 301. a first rotor body; 302. a second rotor body; 4. a circular groove; 5. a containing groove; 6. inserting the groove; 7. mounting blocks; 8. mounting plates, 9 and baffle plates; 10. an accommodating space; 11. a bump; 12. and a support plate.
Detailed Description
The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.
Examples
A centrifugal machine for nucleic acid amplification comprises a shell 1 and a sealing cover which is arranged on the shell 1 in a sealing mode and can open or close the shell 1, wherein an incubation bin and a centrifugal bin 101 are arranged inside the shell 1, a nucleic acid isothermal amplification device is arranged in the incubation bin, and a centrifugal device is arranged in the centrifugal bin 101.
In this embodiment, rotor body is provided with two replacement uses, including first rotor body 301 and second rotor body 302, be provided with two insertion grooves 6 along 2 axial symmetries of pivot on the inside wall of first rotor body 301, plug respectively in two insertion grooves 6 and be provided with installation piece 7, supply platelike container male storage tank 5 has all been seted up to one side that two installation pieces 7 are close to pivot 2, platelike container when centrifugal, only need insert in the storage tank 5, insert installation piece 7 in insertion groove 6 again and can carry out centrifugation, after the centrifugation is accomplished, only need take installation piece 7 away, platelike container get and put, the change all can go on in the centrifuge outside, time saving, and the efficiency is improved.
A plurality of circular slot 4 has been seted up to second rotor body 302's inside wall, the internal diameter of circular slot 4 that sets up along 2 axial symmetries of pivot equals, there are 3 kinds of circular slots 4 that the internal diameter is different in this embodiment, the circular slot 4 that the internal diameter is the biggest is provided with 1 group along 2 axial symmetries of pivot, can place the tubular container that the capacity is 1.5 mL, the circular slot 4 that the internal diameter is the smallest is provided with 5 groups along 2 axial symmetries of pivot, can place the tubular container that the capacity is 25 mu L, circular slot 4 that the internal diameter is placed in the middle is provided with 2 groups along 2 axial symmetries of pivot, can place the tubular container that the capacity is 200 mu L, circular slot 4 that sets up multiple specification supplies the centrifuging tube of size difference to put into, reduce the quantity of rotor, be convenient for preserve.
The outer wall of one end of the second rotor body 302 far away from the rotating shaft 2 is symmetrically provided with mounting plates 8 outwards in a protruding manner, one end of each mounting plate 8 far away from the rotating shaft 2 is provided with a baffle plate 9 towards the direction far away from the second rotor body 302, a containing space 10 for the plate-shaped container to be guided and inserted along the axial direction of the rotating shaft 2 is formed between the two baffle plates 9, the second rotor body 302 can be used for centrifuging the tubular container and centrifuging the plate-shaped container, the containing space 10 is arranged above the round groove 4 arranged on the second rotor body 302, so that the centrifuging of the tubular container and the plate-shaped container can be carried out simultaneously, the centrifuging efficiency is improved, the cost is saved, the surface of the plate-shaped container close to the baffle plates 9 is provided with a guide groove, the surface of the baffle plates 9 close to the plate-shaped container is convexly provided with a convex block 11 which is in guide sliding fit with the guide groove, the convex block between the plate-shaped container and the baffle plates 9 to limit the relative rotation between the plate-shaped container and the baffle plates 9, thereby ensuring that the plate-shaped container synchronously rotates along with the second rotor body 302, the inner side wall of the second rotor body 302 is provided with support plates 12, the upper end surfaces of which are abutted against the bottom surface of the plate-shaped container when the plate-shaped container is inserted, so as to improve the stability of the plate-shaped container when the plate-shaped container is placed, and in the embodiment, 4 support plates 12 are arranged on the inner side wall of the second rotor body 302 below the plate-shaped container.
One side of the baffle 9 close to the plate-shaped container is in a plane shape or is arranged in an arc shape concentric with the rotating shaft 2, the baffle is arranged in a plane shape and is more suitable for the use of the plate-shaped container such as a microarray chip, the baffle is arranged in an arc shape concentric with the rotating shaft 2 and is more suitable for the use of a disc-shaped container such as a microfluidic disc chip, the baffle is arranged in an arc shape concentric with the rotating shaft 2 in the embodiment, so that the second rotor body 302 is more suitable for tubular containers and disc-shaped containers, and the centrifuge is suitable for containers with various shapes and specifications by combining the accommodating groove 5 arranged on the first rotor body 301.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the utility model. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. The utility model provides a centrifuge for nucleic acid amplification, includes casing (1) and in the inside hatching storehouse and centrifugal storehouse (101) that set up of casing (1), is provided with centrifugal device in centrifugal storehouse (101), and centrifugal device includes pivot (2) that vertical rotation set up in centrifugal storehouse (101) and can dismantle the setting on pivot (2) with pivot (2) synchronous rotating's rotor, its characterized in that: the rotor is provided with at least one, and the rotor includes rotor body and the first holding portion that supplies the tubular container to insert and/or the second holding portion that supplies the slabby container to insert that sets up on rotor body, and first holding portion and second holding portion are provided with at least one respectively.
2. The centrifuge for nucleic acid amplification according to claim 1, wherein: the first containing part comprises a plurality of circular grooves (4) formed in the inner side wall of the rotor body, and the inner diameters of the circular grooves (4) symmetrically arranged along the axial direction of the rotating shaft (2) are equal.
3. The centrifuge for nucleic acid amplification according to claim 2, wherein: the inner side wall of the rotor body is provided with at least two circular grooves (4) with different inner diameters.
4. The centrifuge for nucleic acid amplification according to claim 1, wherein: the second containing part comprises containing grooves (5) which are symmetrically arranged on the inner side wall of the rotor body along the axial direction of the rotating shaft (2) and are used for inserting the platy containers.
5. The centrifuge for nucleic acid amplification according to claim 4, wherein: inserting grooves (6) are axially and symmetrically arranged on the inner side wall of the rotor body along the rotating shaft (2), an installing block (7) is detachably arranged in each inserting groove (6), and the containing groove (5) is arranged on the installing block (7).
6. The centrifuge for nucleic acid amplification according to claim 1, wherein: the second accommodating part comprises mounting plates (8) which are symmetrically and outwards convexly arranged on the outer wall of one end, far away from the rotating shaft (2), of the rotor body, baffles (9) which are arranged in the direction far away from the rotor body at one end, far away from the rotating shaft (2), of each mounting plate (8), and accommodating spaces (10) which are formed between the two baffles (9) and used for the plate-shaped containers to be inserted in an axial direction along the rotating shaft (2).
7. The centrifuge for nucleic acid amplification according to claim 6, wherein: linkage structures for driving the plate-shaped container and the rotor body to synchronously rotate are respectively arranged between the two ends of the plate-shaped container and the baffle (9).
8. The centrifuge for nucleic acid amplification according to claim 7, wherein: the linkage structure comprises a guide groove arranged on the surface of the plate-shaped container close to the baffle (9) and a convex block (11) convexly arranged on the surface of the baffle (9) close to the plate-shaped container and matched with the guide groove in a guide sliding way.
9. The centrifuge for nucleic acid amplification according to claim 6, wherein: one side of the baffle (9) close to the plate-shaped container is arranged in a plane shape or an arc shape concentric with the rotating shaft (2).
10. The centrifuge for nucleic acid amplification according to claim 6, wherein: a support plate (12) is provided on the inner side wall of the rotor body, the upper end surface of which abuts against the bottom surface of the plate-like container when the plate-like container is inserted.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122154407.8U CN215655768U (en) | 2021-09-07 | 2021-09-07 | Centrifugal machine for nucleic acid amplification |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122154407.8U CN215655768U (en) | 2021-09-07 | 2021-09-07 | Centrifugal machine for nucleic acid amplification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215655768U true CN215655768U (en) | 2022-01-28 |
Family
ID=79960490
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202122154407.8U Active CN215655768U (en) | 2021-09-07 | 2021-09-07 | Centrifugal machine for nucleic acid amplification |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215655768U (en) |
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2021
- 2021-09-07 CN CN202122154407.8U patent/CN215655768U/en active Active
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