CN215586518U - Microfluid chip, microfluid chip mounting assembly and single cell library preparation system - Google Patents

Abstract

The utility model discloses a micro-fluidic chip, a micro-fluidic chip mounting assembly and a single-cell library preparation system, wherein the micro-fluidic chip comprises a main body and an identification structure, the main body is provided with a first side and a second side which are oppositely arranged, a plurality of accommodating grooves are formed on the first side, at least four of the accommodating grooves are a first raw material groove, a second raw material groove, a third raw material groove and a finished product groove, and a flow channel which is respectively communicated with the first raw material groove, the second raw material groove, the third raw material groove and the finished product groove is formed on the second side, so that raw materials can be accommodated in the finished product groove after flowing into the flow channel and being molded through the first raw material groove, the second raw material groove and the third raw material groove respectively; the identification structure corresponds at least one the holding tank sets up, through the identification structure makes things convenient for operating personnel to the difference the holding tank is distinguished, avoids the error, leads to processing failure, improves preparation efficiency.

Description

Microfluid chip, microfluid chip mounting assembly and single cell library preparation system

Technical Field

The utility model relates to the technical field of microfluid, in particular to a microfluid chip, a microfluid chip mounting assembly and a single cell library preparation system.

Background

In the existing microfluidic chip, different materials are generally wrapped by an oil film, and then a plurality of samples are generated, so that a plurality of accommodating grooves need to be designed to correspond to different raw materials and collect prepared samples.

SUMMERY OF THE UTILITY MODEL

The utility model mainly aims to provide a microfluid chip, a microfluid chip mounting assembly and a single cell library preparation system, and aims to solve the problem that the existing accommodating groove of the microfluid chip is difficult to distinguish.

To achieve the above object, the present invention provides a microfluidic chip comprising:

the main body is provided with a first side and a second side which are oppositely arranged, a plurality of accommodating grooves are formed in the first side, at least four of the accommodating grooves are a first raw material groove, a second raw material groove, a third raw material groove and a finished product groove, and runners at least communicated with the first raw material groove, the second raw material groove, the third raw material groove and the finished product groove are formed in the second side, so that raw materials can flow into the runners through the first raw material groove, the second raw material groove and the third raw material groove respectively, are molded and then are contained in the finished product groove; and the number of the first and second groups,

the mark structure corresponds to at least one of the accommodating grooves.

Optionally, the plurality of accommodating grooves penetrate from the first side to the second side, the second side is provided with at least three grooves, one end of each of the three grooves is communicated with the first raw material groove, the second raw material groove and the third raw material groove in a one-to-one correspondence manner, and the other ends of the three grooves extend to be communicated with the finished product groove after meeting;

the microfluid chip also comprises a cover film, and the cover film is covered on the second side to form the flow channel together with the grooves in an enclosing manner.

Optionally, the identification structure includes an identification symbol disposed on a peripheral side of at least one of the receiving grooves.

Optionally, a plurality of pipelines are formed on the first side in a protruding mode, and a plurality of accommodating grooves are formed in the inner cavities of the pipelines in a one-to-one correspondence mode.

Optionally, the first raw material tank and the finished product tank are respectively arranged on two sides of the main body in the length direction; wherein,

the second raw material groove and the third raw material groove are arranged between the first raw material groove and the finished product groove at intervals; and/or the presence of a gas in the gas,

the surfaces of one sides, close to each other, of the first raw material groove and the finished product groove are arranged in a convex arc surface shape.

Optionally, the pipeline forming the first raw material tank is provided with a first anti-slip part on one side far away from the finished product tank;

and a second anti-skid part is arranged on one side of the pipeline forming the finished product groove, which is far away from the first raw material groove.

Optionally, a plurality of the first anti-slip parts are arranged at intervals along the depth direction of the first raw material groove;

a plurality of second anti-skid parts are arranged at intervals along the depth direction of the finished product groove;

the first anti-slip parts and the second anti-slip parts are at least partially arranged differently to form the identification structure.

The present invention also provides a microfluidic chip mounting assembly comprising:

a carrier plate; and the number of the first and second groups,

the above microfluidic chip, said microfluidic chip comprising:

the main body is arranged on the carrier plate, the main body is provided with a first side and a second side which are oppositely arranged, a plurality of accommodating grooves are formed in the first side, at least four of the accommodating grooves are a first raw material groove, a second raw material groove, a third raw material groove and a finished product groove, and flow channels which are respectively communicated with the first raw material groove, the second raw material groove, the third raw material groove and the finished product groove are formed in the second side, so that raw materials can be contained in the finished product groove after flowing into the flow channels and being molded through the first raw material groove, the second raw material groove and the third raw material groove respectively; and the number of the first and second groups,

the mark structure corresponds to at least one of the accommodating grooves.

Optionally, the microfluidic chip mounting assembly further comprises a positioning portion disposed on the main body and a matching portion disposed on the carrier plate;

the positioning part comprises a first positioning column and a second positioning column which are convexly arranged on the second side;

the matching part comprises a first sliding groove and a second sliding groove which are arranged on the carrier plate, and the extending directions of the first sliding groove and the second sliding groove are arranged in a crossed manner;

the first positioning column is in sliding fit with the first sliding groove, the second positioning column is in sliding fit with the second sliding groove, and the positioning portion and/or the matching portion form the identification structure.

The utility model also provides a single cell library preparation system, which comprises the microfluidic chip mounting assembly, wherein the microfluidic chip mounting assembly comprises:

a carrier plate; and the number of the first and second groups,

the above microfluidic chip, said microfluidic chip comprising:

the main body is arranged on the carrier plate, the main body is provided with a first side and a second side which are oppositely arranged, a plurality of accommodating grooves are formed in the first side, at least four of the accommodating grooves are a first raw material groove, a second raw material groove, a third raw material groove and a finished product groove, and flow channels which are respectively communicated with the first raw material groove, the second raw material groove, the third raw material groove and the finished product groove are formed in the second side, so that raw materials can be contained in the finished product groove after flowing into the flow channels and being molded through the first raw material groove, the second raw material groove and the third raw material groove respectively; and the number of the first and second groups,

the mark structure corresponds to at least one of the accommodating grooves.

According to the technical scheme, a first raw material groove, a second raw material groove, a third raw material groove and a finished product groove in a plurality of accommodating grooves are distinguished through the identification structures, the microfluid is placed in processing equipment, the first raw material groove, the second raw material groove and the third raw material groove are output from respective flow channels under the action of air pressure, and are output into the finished product groove through the flow channels communicated with the finished product groove after reaction, so that a detection sample is obtained, the corresponding accommodating grooves are rapidly identified, errors and processing failures are avoided, and the preparation efficiency is improved.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the structures shown in the drawings without creative efforts.

FIG. 1 is a schematic structural diagram of an embodiment of a microfluidic chip according to the present invention;

FIG. 2 is a schematic front view of the microfluidic chip of FIG. 1;

FIG. 3 is a schematic bottom view of the microfluidic chip of FIG. 1;

FIG. 4 is a schematic diagram of a flow channel of the microfluidic chip of FIG. 1;

FIG. 5 is a schematic structural diagram of a carrier plate engaged with the first slider and the second slider in FIG. 2;

FIG. 6 is a schematic diagram of a plurality of microfluidic chips in FIG. 1 assembled side by side.

The reference numbers illustrate:

reference numerals	Name (R)	Reference numerals	Name (R)
				100	Microfluidic chip	14	Second positioning column
1	Main body	2	First raw material tank
				11	First side	21	First anti-slip part
111	Accommodating tank	3	Second raw material tank
				1111	Pipeline	4	Third raw material groove
1112	Inner cavity	5	Finished product groove
				12	Second side	51	Second anti-slip part
121	Flow passage	6	Support plate
				1211	Groove	61	First chute
1212	Cover film	62	Second chute
				13	First positioning column

The implementation, functional features and advantages of the objects of the present invention will be further explained with reference to the accompanying drawings.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

It should be noted that, if directional indication is involved in the embodiment of the present invention, the directional indication is only used for explaining the relative positional relationship, the motion situation, and the like between the components in a certain posture, and if the certain posture is changed, the directional indication is changed accordingly.

In addition, if there is a description of "first", "second", etc. in an embodiment of the present invention, the description of "first", "second", etc. is for descriptive purposes only and is not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In addition, technical solutions between various embodiments may be combined with each other, but must be realized by a person skilled in the art, and when the technical solutions are contradictory or cannot be realized, such a combination should not be considered to exist, and is not within the protection scope of the present invention.

In the existing microfluidic chip, different materials are generally wrapped by an oil film, and then a plurality of samples are generated, so that a plurality of accommodating grooves need to be designed to correspond to different raw materials and collect prepared samples.

In view of the above, the present invention provides a microfluidic chip, a microfluidic chip mounting assembly and a single-cell library preparation system, so as to solve the problem of difficulty in accommodating groove resolution of the existing microfluidic chip. Fig. 1 to 4 are views showing an embodiment of a microfluidic chip provided in the present invention, fig. 5 is a view showing an embodiment of a carrier for mounting the microfluidic chip in fig. 1 provided in the present invention, and fig. 6 is a view showing an assembly of a plurality of the microfluidic chips provided in the present invention.

Referring to fig. 1 and 4, the microfluidic chip 100 includes a main body 1 and a mark structure, wherein the main body 1 has a first side 11 and a second side 12 opposite to each other, the first side 11 is formed with a plurality of accommodating grooves 111, at least four of the accommodating grooves 111 are a first raw material groove 2, a second raw material groove 3, a third raw material groove 4 and a finished product groove 5, the second side 12 is formed with a flow channel 121 respectively communicating with at least the first raw material groove 2, the second raw material groove 3, the third raw material groove 4 and the finished product groove 5, so that raw materials respectively flow into the flow channel 121 through the first raw material groove 2, the second raw material groove 3 and the third raw material groove 4 and are accommodated in the finished product groove 5 after being molded; the identification structure is disposed corresponding to at least one of the accommodating grooves 111.

In the technical scheme of the utility model, a first raw material groove 2, a second raw material groove 3, a third raw material groove 4 and a finished product groove 5 in a plurality of accommodating grooves 111 are distinguished through the identification structures, the microfluid is placed in processing equipment, the first raw material groove 2, the second raw material groove 3 and the third raw material groove 4 are output from respective flow channels 121 under the action of air pressure, and are output into the finished product groove 5 through the flow channel 121 communicated with the finished product groove 5 after reaction to obtain a detection sample, so that the corresponding accommodating grooves 111 are rapidly identified, errors and processing failures are avoided, and the preparation efficiency is improved.

Specifically, referring to fig. 1 to 4, the plurality of accommodating grooves 111 penetrate from the first side 11 to the second side 12, the second side 12 is provided with at least three grooves 1211, one end of each of the three grooves 1211 is in one-to-one correspondence with the first raw material groove 2, the second raw material groove 3, and the third raw material groove 4, and the other ends of the three grooves 1211 extend to be communicated with the finished product groove 5 after meeting; the microfluidic chip 100 further includes a cover film 1212, wherein the cover film 1212 is disposed on the second side 12 to form the flow channel 121 together with each of the grooves 1211, so as to facilitate processing and assembling the microfluidic chip 100.

In order to distinguish the accommodating grooves 111, in an embodiment of the present invention, the identification structure includes an identification symbol disposed on a peripheral side of at least one of the accommodating grooves 111, and the identification symbol may be a number, a color, a character, or a special symbol, which is not limited thereto. The mark symbol is arranged, so that the structure is simple and the distinguishing is easy.

Referring to fig. 1, the accommodating groove 111 may be formed in various ways, and in one embodiment of the present invention, a plurality of pipes 1111 are protrudingly formed on the first side 11, a plurality of accommodating grooves 111 are formed by the pipes 1112 of the plurality of pipes 1111 in a one-to-one correspondence, and the plurality of pipes 1111, the raw materials in the holding tank 111 are forced to flow through the flow channel 121 to start the reaction synthesis under the action of the air pressure, so that the structure is simple, compared with another embodiment of the present invention, namely, a plurality of holes are punched on a bump to form the accommodating groove 111, and the pipeline 1111 is arranged, so that the structure is simpler and lighter, and a plurality of pipes 1111 are independently provided, the pipes 1111 may be further configured to be detachably mounted to the first side 11, so set up, if a certain pipeline 1111 goes wrong, but in time change avoids whole change, practices thrift the cost.

It is emphasized that the pipe 1111 is detachably mounted on the first side 11, and it is necessary to ensure the sealing property of the pipe 1111 connected to the main body 1, in an embodiment of the present invention, a sealing gasket is provided between the pipe 1111 and the first side 11.

Referring to fig. 1 to 2, in order to distinguish the plurality of receiving grooves 111, the plurality of receiving grooves 111 may be arranged at intervals along a fixed direction, and the receiving grooves 111 at the head end or the tail end are configured such that raw materials received by each receiving groove 111 can be determined according to an arrangement sequence of each receiving groove 111 preset in manufacturing of the microfluidic chip 100, specifically, in an embodiment of the present invention, the first raw material groove 2 and the finished product groove 5 are respectively disposed at two sides of the main body 1 in a length direction; the second raw material groove 3 and the third raw material groove 4 are arranged between the first raw material groove 2 and the finished product groove 5 at intervals, so that the microfluidic chip 100 can be picked and placed by clamping the outer wall of the pipeline of the first raw material groove 2 and the outer wall of the pipeline of the finished product groove 5 which are farthest away, and the operation is simple.

Referring to fig. 4, in order to facilitate the transportation or reception of each of the accommodating grooves 111 through the flow channel 121, a guiding region should be disposed at a connection portion of the accommodating groove 111 and the flow channel 121, that is, the width of the accommodating groove 111 should be gradually reduced from a direction of the accommodating groove 111 approaching the flow channel 121, for example, the accommodating groove 111 may be configured as a circle, or may be configured as a triangle, which is not limited thereto.

In order to save as much material as possible for each of the tubes 1111 itself, the wall thickness of each of the tubes 1111 should be as thin as possible to save cost while satisfying strength requirements, and therefore, each of the tubes 1111 should be formed similarly to the shape of the inner cavity of the corresponding receiving groove 111.

Referring to fig. 1, in order to conveniently clamp the microfluidic chip 100, one surface of the pipe 1111, which is shaped for clamping, may be a flat end surface so as to facilitate clamping, and therefore, the surface of one side of the first raw material tank 2 and the surface of one side of the finished product tank 5, which are close to each other, may be convex.

Further, for the convenience of clamping, the pipe 1111 forming the first raw material tank 2 is provided with a first non-slip portion 21 at a side away from the finished product tank 5; the pipeline 1111 forming the finished product tank 5 is provided with a second anti-slip part 51 at one side far away from the first raw material tank 2, so that the pipeline is not easy to fall off in the clamping process and is convenient to mount and dismount.

Referring to fig. 1, in order to easily distinguish the head ends or the tail ends of a plurality of accommodating grooves 111 arranged at intervals along the length direction of a main body 1, in the present invention, a plurality of first anti-slip parts 21 (not shown) are arranged at intervals along the depth direction of a first raw material groove 2; a plurality of second anti-slip parts 51 (not shown in the figure) are arranged at intervals along the depth direction of the finished product groove 5; the first anti-slip parts 21 and the second anti-slip parts 51 are at least partially arranged differently to form the identification structure, so that the first raw material tank 2 and the finished product tank 5 can be quickly distinguished.

It should be noted that the form of the first anti-slip part 21 and the second anti-slip part 51 is not limited, and it is within the protection scope of the present invention as long as it can be conveniently taken and placed, for example, it may be a plurality of protrusions or a plurality of grooves to increase the roughness of the corresponding position.

It should be noted that, in the present invention, the raw materials placed in the first raw material tank 2, the second raw material tank 3, and the third raw material tank 4 are not limited, and may be designed according to actual needs, and correspondingly, a device for separating a part of the raw materials may be added in the first raw material tank 2, the second raw material tank 3, and the third raw material tank 4, so as to synthesize a plurality of separate samples, for example, in an embodiment of the present invention, the first raw material tank 2, the second raw material tank 3, and the third raw material tank 4 respectively and correspondingly contain oil, cells, and magnetic beads, wherein the third raw material tank 4 for containing magnetic beads may further be provided with a filter column, which is well known by those skilled in the art and is not described herein.

The present invention further provides a microfluidic chip mounting assembly, which includes a carrier 6 and all technical features of the microfluidic chip 100, and therefore, the assembly also has technical effects brought by all the technical features, which are not described in detail herein, wherein the main body 1 is mounted on the carrier 6.

Specifically, referring to fig. 2, fig. 3 and fig. 5, the microfluidic chip mounting assembly further includes a positioning portion disposed on the main body 1 and a matching portion disposed on the carrier plate 6; the positioning part comprises a first positioning column 13 and a second positioning column 14 which are convexly arranged on the second side 12; the matching part comprises a first sliding groove 61 and a second sliding groove 62 which are arranged on the carrier plate 6, and the extending directions of the first sliding groove 61 and the second sliding groove 62 are arranged in a crossed manner; the first positioning column 13 is slidably engaged with the first sliding groove 61, the second positioning column 14 is slidably engaged with the second sliding groove 62, and the positioning portion and/or the engaging portion constitute the identification structure, so that when positioning and mounting are performed, the first positioning column 13 can be inserted into the first sliding groove 61, at this moment, the first sliding groove 61 limits the movement of the microfluidic chip 100 along the width direction of the first sliding groove 61, the relative position of the first positioning column 13 in the first sliding groove 61 is continuously adjusted, so that the second positioning column 14 can be inserted into the second sliding groove 62, at this moment, the second sliding groove 62 limits the movement of the microfluidic chip along the width direction of the second sliding groove 62, and the combination of the two different directions limits the movement, so that the microfluidic chip 100 is fixed in the horizontal direction, and the microfluidic chip 100 is prevented from shaking in the horizontal direction, the transportation of raw materials and the synthesis of samples in each accommodating groove 111 are influenced, and the difference of the positions of the first mounting column and the second mounting column on the main body 1 is also convenient for an operator to identify the corresponding accommodating grooves 111, and finally, a plurality of microfluidic chips are arranged to be assembled and mounted as shown in fig. 6.

Further, referring to fig. 2, in order to conveniently and quickly identify whether the microfluidic chip 100 is installed incorrectly, the depths of the first sliding groove 61 and the second sliding groove 62 are set to be different (not shown in the figure), the first positioning column 13 is disposed corresponding to the first sliding groove 61, the second positioning column 14 is disposed corresponding to the second sliding groove 62, when the microfluidic chip is normally installed, the first side 11 of the main body 1 should be in a horizontal state, and if the installation is reversed, the first side 11 of the main body 1 should be in an inclined state, at this moment, an operator can timely find a problem and reinstall the microfluidic chip 100, so that the error rate can be reduced.

Of course, the first positioning column 13 and the second positioning column 14 may also be disposed on the carrier 6, and correspondingly, the first sliding groove 61 and the second sliding groove 62 are correspondingly disposed on the second side 12 of the main body 1, and the mounting manner is similar, which is not repeated herein.

It should be noted that the number, position and shape of the first positioning posts 13 and the second positioning posts 14, and the number, position and shape of the first sliding grooves 61 and the second sliding grooves 62 are not limited, and the microfluidic chip 100 can be limited to move in the horizontal direction, which is within the protection scope of the present invention.

It should be emphasized that the horizontal direction mentioned above is not limited to a completely horizontal direction, and may be inclined as long as it can limit the air pressure to be added to the receiving groove 111 when the microfluidic chip 100 is mounted on the carrier plate 6, and the bayonet of the receiving groove 111 faces the air outlet of the air pressure generating device.

The utility model also provides a single cell library preparation system, which comprises all the technical characteristics of the microfluidic chip mounting assembly, so that the single cell library preparation system also has the technical effects brought by all the technical characteristics, and the details are not repeated.

The above description is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention, and all modifications and equivalents of the present invention, which are made by the contents of the present specification and the accompanying drawings, or directly/indirectly applied to other related technical fields, are included in the scope of the present invention.

Claims (10)

1. A microfluidic chip, comprising:

the main body is provided with a first side and a second side which are oppositely arranged, a plurality of accommodating grooves are formed in the first side, at least four of the accommodating grooves are a first raw material groove, a second raw material groove, a third raw material groove and a finished product groove, and runners at least communicated with the first raw material groove, the second raw material groove, the third raw material groove and the finished product groove are formed in the second side, so that raw materials can flow into the runners through the first raw material groove, the second raw material groove and the third raw material groove respectively, are molded and then are contained in the finished product groove; and the number of the first and second groups,

the mark structure corresponds to at least one of the accommodating grooves.

2. The microfluidic chip according to claim 1, wherein the plurality of holding grooves pass through from the first side to the second side, the second side has at least three grooves, one end of each of the three grooves is in one-to-one correspondence with the first material groove, the second material groove, and the third material groove, and the other ends of the three grooves extend to be in communication with the product groove after meeting;

the microfluid chip also comprises a cover film, and the cover film is covered on the second side to form the flow channel together with the grooves in an enclosing manner.

3. The microfluidic chip of claim 1, wherein said identification structure comprises an identification symbol disposed on a peripheral side of at least one of said receiving grooves.

4. The microfluidic chip of claim 1, wherein a plurality of channels are formed protruding from said first side, and a plurality of said receiving slots are formed in a one-to-one correspondence with a plurality of lumens of said channels.

5. The microfluidic chip according to claim 4, wherein said first stock tank and said finished tank are provided on both sides of said body in a longitudinal direction thereof; wherein,

the second raw material groove and the third raw material groove are arranged between the first raw material groove and the finished product groove at intervals; and/or the presence of a gas in the gas,

the surfaces of one sides, close to each other, of the first raw material groove and the finished product groove are arranged in a convex arc surface shape.

6. The microfluidic chip according to claim 5, wherein said conduit forming said first raw material tank is provided with a first non-slip portion on a side away from said finished product tank;

and a second anti-skid part is arranged on one side of the pipeline forming the finished product groove, which is far away from the first raw material groove.

7. The microfluidic chip according to claim 6, wherein a plurality of the first non-slip portions are provided at intervals in a depth direction of the first raw material tank;

a plurality of second anti-skid parts are arranged at intervals along the depth direction of the finished product groove;

the first anti-slip parts and the second anti-slip parts are at least partially arranged differently to form the identification structure.

8. A microfluidic chip mounting assembly, comprising:

a carrier plate; and the number of the first and second groups,

the microfluidic chip of any one of claims 1 to 7, said body being mounted on said carrier plate.

9. The microfluidic chip mounting assembly of claim 8, further comprising a positioning portion disposed on the main body and a mating portion disposed on the carrier plate;

the positioning part comprises a first positioning column and a second positioning column which are convexly arranged on the second side;

the matching part comprises a first sliding groove and a second sliding groove which are arranged on the carrier plate, and the extending directions of the first sliding groove and the second sliding groove are arranged in a crossed manner;

the first positioning column is in sliding fit with the first sliding groove, the second positioning column is in sliding fit with the second sliding groove, and the positioning portion and/or the matching portion form the identification structure.

10. A single cell library preparation system comprising the microfluidic chip mounting assembly of any one of claims 8 to 9.

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