CN215560375U

CN215560375U - Autologous adipose-derived stem cell in-vitro culture device

Info

Publication number: CN215560375U
Application number: CN202121528973.4U
Authority: CN
Inventors: 李霞; 韩萍; 左钧
Original assignee: Dongying Fengqi Biotechnology Development Co ltd
Current assignee: Dongying Fengqi Biotechnology Development Co ltd
Priority date: 2021-07-07
Filing date: 2021-07-07
Publication date: 2022-01-18
Anticipated expiration: 2031-07-07

Abstract

The utility model discloses an autologous adipose-derived stem cell in-vitro culture device, which comprises an incubator, wherein a cover door is hinged on one side of the incubator, two groups of side driving mechanisms which are symmetrically distributed are arranged on one side of the inner lower wall of the incubator, a middle driving mechanism is fixedly arranged at the lower ends of the opposite sides of the two groups of side driving mechanisms, a plurality of groups of clamping assemblies which are distributed at equal intervals are arranged on one side of each side driving mechanism, and a culture bottle body is movably clamped in each clamping assembly; according to the utility model, a plurality of groups of culture bottle bodies are clamped and fixed in a semi-wrapping manner by arranging a plurality of groups of clamping assemblies, so that a large amount of in-vitro culture is carried out on autologous adipose-derived stem cells, the stability of the culture bottle bodies is improved, the stable automatic rotation of the subsequent culture bottle bodies is facilitated, and the efficiency of the in-vitro culture of the autologous adipose-derived stem cells is further improved.

Description

Autologous adipose-derived stem cell in-vitro culture device

Technical Field

The utility model relates to the technical field of in-vitro cell culture, in particular to an in-vitro culture device for autologous adipose-derived stem cells.

Background

The adipose-derived stem cells are mesenchymal stem cells derived from adipose tissues, are wide in source and simple in extraction mode, can be extracted by directly performing liposuction on the adipose tissues, are high in extraction mode efficiency, and are higher in proliferation rate of in vitro culture, and the adipose-derived stem cells mainly restore the cell repair function of the tissues, promote the regeneration of cells, can effectively resist aging, and improve diseases such as sub-health and premature senility;

in the prior art, adipose-derived stem cells are cultured in vitro, usually by using a tissue block culture method, in the culture process, adipose tissues are cut into pieces, then the tissue blocks are uniformly arranged on the bottom wall of a culture bottle by using a suction pipe, then the culture bottle is required to be manually turned over to add culture solution, the culture bottle is horizontally placed in a culture device, and after 2 to 3 hours, the culture bottle is manually turned over to allow the culture solution to be immersed in the tissue blocks for continuous culture; when the culture device is used for carrying out in-vitro culture on the adipose-derived stem cells, the culture bottle needs to be turned manually, the operation is troublesome, and culture tissues of the adipose-derived stem cells are easily polluted by manually extending into the culture device for turning, so that a novel autologous adipose-derived stem cell in-vitro culture device is provided for solving the problems.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide an autologous adipose-derived stem cell in-vitro culture device to solve the problems in the background technology.

In order to achieve the purpose, the utility model provides the following technical scheme: an autologous adipose-derived stem cell in-vitro culture device comprises an incubator, wherein a cover door is hinged to one side of the incubator, two groups of side driving mechanisms which are symmetrically distributed are arranged on one side of the inner lower wall of the incubator, middle driving mechanisms are fixedly arranged at the lower ends of the opposite sides of the two groups of side driving mechanisms, a plurality of groups of clamping assemblies which are distributed equidistantly are arranged on one side of each side driving mechanism, and culture bottle bodies are movably clamped in the clamping assemblies;

the centre gripping subassembly includes the underframe, the spout has been seted up to top one side of underframe, the slip card is equipped with the drive block in the spout, one side middle part fixed mounting of drive block has the pull rod, the outside movable sleeve of pull rod is equipped with reset spring, the top fixed mounting of drive block has movable clamp plate, one side fixed mounting that the drive block was kept away from on the underframe top has fixed clamp plate, blake bottle body activity joint is moving clamp plate and is deciding between the clamp plate.

As a preferred technical scheme of the utility model, the movable clamping plate and the fixed clamping plate are both L-shaped structures and are symmetrically distributed.

As a preferable technical solution of the present invention, one side of the pull rod passes through the bottom frame and is fixedly mounted with the sleeve.

As a preferable technical scheme of the utility model, the upper part and the lower part of the driving block are respectively movably inserted with a guide rod, and the guide rods are fixedly arranged on the inner wall of the sliding chute.

As a preferred technical scheme of the utility model, the side driving mechanism comprises a mechanism outer frame, a plurality of groups of driving rods corresponding to the clamping assemblies are rotatably inserted into the mechanism outer frame, one side of each driving rod penetrates through the mechanism outer frame and is fixedly installed with one side of the corresponding bottom frame, a driving shaft is rotatably installed at the bottom of the mechanism outer frame, transmission chain wheels are fixedly sleeved on the outer sides of the driving rods, the outer sides of the plurality of groups of transmission chain wheels are connected with transmission chains in a meshed mode, and belt pulley transmission sets are fixedly sleeved on the outer walls of the driving shafts and the driving rod at the lowest side.

As a preferable technical scheme of the utility model, one side of the mechanism outer frame is fixedly provided with a stabilizing frame.

As a preferable technical scheme of the utility model, the middle driving mechanism comprises a middle frame, the middle frame is fixedly arranged at the lower ends of the opposite sides of the two groups of mechanism outer frames, a motor is fixedly arranged at one side of the middle frame, the output end of the motor extends into the middle frame and is fixedly provided with a driving chain wheel, and the outer side of the driving chain wheel and the outer sides of the two groups of driving shafts are connected with a chain transmission set in a meshing manner.

As a preferable technical scheme of the utility model, a protective frame matched with the motor for use is fixedly arranged in the middle of one side of the middle frame.

Compared with the prior art, the utility model has the beneficial effects that:

1. according to the utility model, multiple groups of culture bottle bodies are clamped and fixed in a semi-wrapping manner by arranging multiple groups of clamping assemblies, so that multiple amounts of autologous adipose-derived stem cells are cultured in vitro, the stability of the culture bottle bodies is improved, the subsequent stable automatic rotation of the culture bottle bodies is facilitated, and the efficiency of the autologous adipose-derived stem cell in vitro culture is further improved;

2. side actuating mechanism and well actuating mechanism are used in the cooperation, control and starter motor drive sprocket rotates, thereby the driven sprocket of drive both sides and the synchronous rotation of drive shaft that corresponds, the actuating lever of synchronous drive downside rotates, and then rotate in step through drive chain drive multiunit actuating lever, thereby multiunit centre gripping subassembly and the blake bottle body that synchronous drive corresponds carry out the automatic rotation, multiunit blake bottle body synchronous pivoted efficiency has been improved, and can effectively reduce the cultivation pollution to adipose stem cell culture tissue, and then the efficiency of autologous adipose stem cell external culture has been improved.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a cross-sectional view of the structure of the present invention;

FIG. 3 is a schematic view of the structural connection between the clamping assembly and the main body of the culture flask according to the present invention;

FIG. 4 is a schematic view of the structural connection between the side driving mechanism and the middle driving mechanism and the clamping assembly according to the present invention.

In the figure: 1. an incubator; 2. covering the door; 4. a side drive mechanism; 5. a middle driving mechanism; 6. a clamping assembly; 7. a culture bottle body; 8. a stabilizing frame; 61. a bottom frame; 611. a chute; 62. a drive block; 63. a pull rod; 631. a sleeve; 64. a guide bar; 65. a return spring; 66. fixing the clamping plate; 67. a movable clamping plate; 41. a mechanism outer frame; 42. a drive rod; 43. a drive shaft; 44. a drive sprocket; 441. a drive chain; 45. a pulley transmission set; 51. a middle frame; 52. a protective frame; 53. a motor; 531. a drive sprocket; 54. and a chain transmission set.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-4, the present invention provides a technical solution: an autologous adipose-derived stem cell in-vitro culture device comprises an incubator 1, a cover door 2 is mounted on one side of the incubator 1 through hinges, two groups of side driving mechanisms 4 which are symmetrically distributed are arranged on one side of the inner lower wall of the incubator 1, a middle driving mechanism 5 is fixedly mounted at the lower ends of the opposite sides of the two groups of side driving mechanisms 4, a plurality of groups of clamping assemblies 6 which are equidistantly distributed are arranged on one side of each side driving mechanism 4, a culture bottle body 7 is movably clamped in each clamping assembly 6, a plurality of groups of culture bottle bodies 7 are clamped and fixed through the plurality of groups of clamping assemblies 6, a large amount of autologous adipose-derived stem cells are cultured in vitro, and the culture efficiency of the autologous adipose-derived stem cells is improved;

the clamping assembly 6 comprises a bottom frame 61, a sliding groove 611 is formed in one side of the top end of the bottom frame 61, a driving block 62 is slidably clamped in the sliding groove 611, a pull rod 63 is fixedly installed in the middle of one side of the driving block 62, and a sleeve 631 is fixedly installed on one side of the pull rod 63 through the bottom frame 61; the outer side of the pull rod 63 is movably sleeved with a return spring 65, the return spring 65 is positioned between the driving block 62 and the inner wall of the sliding groove 611, the sleeve 631 is pulled manually, the driving rod 63 drives the driving block 62 to slide in the sliding groove 611 and extrude the return spring 65, the top end of the driving block 62 is fixedly provided with a movable clamping plate 67, one side of the top end of the bottom frame 61, which is far away from the driving block 62, is fixedly provided with a fixed clamping plate 66, the movable clamping plate 67 and the fixed clamping plate 66 are both L-shaped structures, the movable clamping plate 67 and the fixed clamping plate 66 are symmetrically distributed, and the distance between the movable clamping plate 67 and the fixed clamping plate 66 is changed by driving the driving block 62 to slide in the sliding groove 611; 7 activity joints of blake bottle body are moving between splint 67 and the fixed splint 66, in use, increase the distance between splint 67 and the fixed splint 66, place blake bottle body 7 between moving splint 67 and fixed splint 66, loosen sleeve 631, reset spring 65 resets, move the distance between splint 67 and the fixed splint 66 and reduce, move blake bottle body 7 activity joints between splint 67 and the fixed splint 66, and L type structure through moving splint 67 and fixed splint 66, it is fixed to carry out half-wrapped formula centre gripping to blake bottle body 7, thereby the stability of blake bottle body 7 has been improved, be convenient for follow-up blake bottle body 7 stable automatic rotation, prevent that manual rotation from causing the cultivation pollution to fat stem cell culture tissue, and then the efficiency of the external cultivation of autologous fat stem cell has been promoted.

The upper and lower parts of the driving block 62 are respectively movably inserted with a guide rod 64, the guide rods 64 are fixedly installed on the inner wall of the sliding groove 611, and the stability of the driving block 62 sliding in the sliding groove 611 is increased.

The side driving mechanism 4 comprises a mechanism outer frame 41, a plurality of groups of driving rods 42 corresponding to the clamping assemblies 6 are inserted in the mechanism outer frame 41 in a rotating manner, one side of each driving rod 42 penetrates through the mechanism outer frame 41 and is fixedly installed with one side of the corresponding bottom frame 61, a driving shaft 43 is installed at the bottom of the mechanism outer frame 41 in a rotating manner, the outer sides of the driving rods 42 are fixedly sleeved with a driving chain wheel 44, the outer sides of the plurality of groups of driving chain wheels 44 are connected with a driving chain 441 in a meshing manner, and the outer walls of the driving shaft 43 and the driving rod 42 at the lowest side are fixedly sleeved with a belt wheel transmission group 45, wherein the belt wheel transmission group 45 comprises two groups of belt wheels and a driving belt movably sleeved on the outer sides of the two groups of belt wheels, the two groups of belt wheels are respectively and fixedly sleeved on the outer walls of the driving shaft 43 and the driving rod 42 at the lowest side, the driving rod 42 at the lowest side is driven to rotate synchronously by the driving chain 43, thereby multiunit centre gripping subassembly 6 and the blake bottle body 7 that the synchronous drive corresponds carry out the automatic rotation, have improved the synchronous pivoted efficiency of multiunit blake bottle body 7, and can effectively reduce the cultivation pollution to adipose stem cell culture tissue, and then improved the efficiency of autologous adipose stem cell in vitro cultivation.

And one side of the mechanism outer frame 41 is fixedly provided with a stabilizing frame 8, so that the stability of the two groups of side driving mechanisms 4 placed in the incubator 1 is improved.

Well actuating mechanism 5 includes center 51, center 51 fixed mounting is at the opposite side lower extreme of two sets of mechanism frames 41, one side fixed mounting of center 51 has motor 53, the output of motor 53 extends into center 51 and fixed mounting has drive sprocket 531, the outside meshing of drive sprocket 531 and the outside of two sets of drive shafts 43 is connected with chain drive group 54, wherein, chain drive group 54 includes two sets of driven sprocket and the chain of meshing connection in the two sets of driven sprocket outsides, two sets of driven sprocket respectively fixed cup joints the drive shaft 43 outsides in two sets of side actuating mechanism 4, and drive sprocket 531 and this set of chain meshing connection, drive sprocket 531 and rotate through control and starter motor 53, thereby the driven sprocket and the synchronous rotation of two sets of drive shafts 43 of drive both sides.

A protective frame 52 matched with the motor 53 for use is fixedly arranged in the middle of one side of the middle frame 51 to protect the motor 53.

The working principle is as follows: the utility model relates to an autologous adipose-derived stem cell in-vitro culture device, when in use, a plurality of groups of culture bottle bodies 7 are respectively clamped and fixed in corresponding clamping components 6 in a half-wrapping manner, then, a plurality of groups of the clamped culture bottle bodies 7 are placed in the incubator 1 for culture, when the culture bottle bodies 7 need to be overturned subsequently, the motor 53 is controlled and started to drive the driving chain wheel 531 to rotate, thereby driving the driven sprockets at both sides and the corresponding driving shafts 43 to rotate synchronously, synchronously driving the driving rod 42 at the lowest side to rotate, and then the driving rods 42 are driven by the driving chain 441 to synchronously rotate, so as to synchronously drive the corresponding clamping assemblies 6 and the culture bottle bodies 7 to automatically rotate, thereby improving the efficiency of synchronously rotating the culture bottle bodies 7, and the culture pollution to the adipose-derived stem cell culture tissue can be effectively reduced, so that the efficiency of the in-vitro culture of the autologous adipose-derived stem cells is improved.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides an autologous adipose-derived stem cell in vitro culture device, includes incubator (1), its characterized in that: a cover door (2) is hinged on one side of the incubator (1), two groups of side driving mechanisms (4) which are symmetrically distributed are arranged on one side of the inner lower wall of the incubator (1), middle driving mechanisms (5) are fixedly arranged at the lower ends of the opposite sides of the two groups of side driving mechanisms (4), a plurality of groups of clamping assemblies (6) which are distributed equidistantly are arranged on one side of each side driving mechanism (4), and an incubator bottle body (7) is movably clamped in each clamping assembly (6);

centre gripping subassembly (6) are including underframe (61), spout (611) have been seted up to the top one side of underframe (61), slide card is equipped with drive block (62) in spout (611), one side middle part fixed mounting of drive block (62) has pull rod (63), the outside activity cover of pull rod (63) is equipped with reset spring (65), the top fixed mounting of drive block (62) has movable splint (67), one side fixed mounting that drive block (62) were kept away from on underframe (61) top has decides splint (66), blake bottle body (7) activity joint is moving splint (67) and deciding between splint (66).

2. The in vitro culture device of autologous adipose-derived stem cells according to claim 1, wherein: the movable clamping plate (67) and the fixed clamping plate (66) are both L-shaped structures, and the movable clamping plate (67) and the fixed clamping plate (66) are symmetrically distributed.

3. The in vitro culture device of autologous adipose-derived stem cells according to claim 1, wherein: one side of the pull rod (63) penetrates through the bottom frame (61) and is fixedly provided with a sleeve (631).

4. The in vitro culture device of autologous adipose-derived stem cells according to claim 1, wherein: the upper portion and the lower portion of the driving block (62) are respectively movably inserted with a guide rod (64), and the guide rods (64) are fixedly installed on the inner wall of the sliding groove (611).

5. The in vitro culture device of autologous adipose-derived stem cells according to claim 1, wherein: side actuating mechanism (4) are including mechanism frame (41), rotate in mechanism frame (41) and insert and be equipped with multiunit actuating lever (42) that correspond with centre gripping subassembly (6), mechanism frame (41) and underframe (61) one side fixed mounting that corresponds are passed to one side of actuating lever (42), drive shaft (43) are installed in the bottom rotation of mechanism frame (41), the outside of actuating lever (42) is all fixed the cover and is equipped with drive sprocket (44), multiunit the outside meshing of drive sprocket (44) is connected with drive chain (441), drive shaft (43) and the fixed cover of actuating lever (42) outer wall of downside are equipped with belt pulley transmission group (45).

6. The in vitro culture device of autologous adipose-derived stem cells according to claim 5, wherein: and one side of the mechanism outer frame (41) is fixedly provided with a stabilizing frame (8).

7. The in vitro culture device of autologous adipose-derived stem cells according to claim 5, wherein: the middle driving mechanism (5) comprises a middle frame (51), the middle frame (51) is fixedly installed at the lower ends of the two opposite sides of the two groups of mechanism outer frames (41), a motor (53) is fixedly installed at one side of the middle frame (51), the output end of the motor (53) extends into the middle frame (51) and is fixedly installed with a driving chain wheel (531), and the outer side of the driving chain wheel (531) and the outer side of the two groups of driving shafts (43) are connected with a chain transmission group (54) in a meshed mode.

8. The in vitro culture device of autologous adipose-derived stem cells according to claim 7, wherein: and a protective frame (52) matched with the motor (53) for use is fixedly arranged in the middle of one side of the middle frame (51).