CN215517228U - Protein purification sampling bag - Google Patents

Abstract

The utility model relates to the field of protein purification, in particular to a protein purification sample loading bag which comprises a sample loading bag, a sample loading pipe, a filter connector, a connecting assembly and a sealing cover opening, wherein the bottom of the sample loading bag is provided with a sample outlet pipe and a sample inlet pipe, the filter connector is fixedly arranged on the sample inlet pipe, the connecting assembly is fixedly arranged on the sample outlet pipe, the top of the sample loading pipe is connected with the bottom of the connecting assembly, and the sealing cover opening is fixedly arranged at the bottom of the sample loading pipe.

Description

Protein purification sampling bag

Technical Field

The utility model relates to the field of protein purification, and in particular relates to a protein purification sample loading bag.

Background

The separation and purification of proteins is widely used in biochemical research applications and is an important operation technology. A typical eukaryotic cell may contain thousands of different proteins, some very abundant, some containing only a few copies. To study a protein, the protein must first be purified from other proteins and non-protein molecules

The sample loading is generally placed in a sample loading bottle during protein purification, the sample loading is connected through a pipe, the sample loading needs to be filtered before sample loading, the sample loading speed is controlled by a peristaltic pump, the process is complex, and the joint of the pipe and a bottle opening is easy to pollute.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a protein purification sample loading bag.

In order to achieve the purpose, the utility model adopts the following technical scheme:

the utility model provides a protein purification goes up appearance bag, including going up the appearance bag, going up appearance pipe, filtering connector, coupling assembling and sealed flap, the bottom of going up the appearance bag is provided with out appearance pipe and advances appearance pipe, filtering connector fixed mounting is on advancing appearance pipe, coupling assembling fixed mounting is on going out appearance pipe, the top of going up the appearance pipe is connected with coupling assembling's bottom, sealed flap fixed mounting is in the bottom of last appearance pipe, the top of going up the appearance bag is provided with plastics and hangs the rope.

Further, the filter connector comprises a sealing cover, a filter pipe and a filter membrane, a first bag connecting pipe is arranged at the top of the filter pipe, the first bag connecting pipe is inserted into the sample injection pipe, the outer edge of the first bag connecting pipe is in sealing connection with the inner edge of the sample injection pipe, the filter membrane is fixedly installed at the top of the inner edge of the filter pipe, external threads are formed in the bottom of the outer edge of the filter pipe, internal threads connected with the external threads are formed in the inner edge of the sealing cover, and the sealing cover is connected with the filter pipe in a meshed mode.

Further, the connecting assembly comprises a second bag connecting pipe and a first connecting pipe, the top of the second bag connecting pipe is inserted into the sample outlet pipe, the outer edge of the second bag connecting pipe is connected with the inner edge of the sample outlet pipe in a sealing mode, the outer edge of the bottom of the second bag connecting pipe is provided with an outer thread, the inner edge of the top of the first connecting pipe is provided with an inner thread connected with the second bag connecting pipe through the outer thread, the first connecting pipe is connected with the second bag connecting pipe in a meshing mode, the bottom of the first connecting pipe is provided with a first sealing pipe, the first sealing pipe is inserted into the sample outlet pipe, and the outer edge of the first sealing pipe is provided with a plurality of first arc-shaped clamping rings clamped with the top of the sample outlet pipe in a sealing mode.

Further, the sealing cover opening comprises a rubber pad, a second connecting pipe and an aluminum cover, a second sealing pipe is arranged at the top of the second connecting pipe, the second sealing pipe is inserted into the sample injection pipe, a plurality of second arc-shaped clamping rings clamped with the bottom of the sample injection pipe in a sealing mode are arranged on the outer edge of the second sealing pipe, a third arc-shaped clamping ring is arranged on the outer edge of the second connecting pipe, the rubber pad is inserted into the inner edge of the second connecting pipe, the aluminum cover is fixedly arranged on the outer edge of the second connecting pipe, and a circular notch for inserting the needle pipe is formed in the bottom of the aluminum cover.

Further, the circular breach of the bottom of aluminium lid is provided with sealed sticker, and sealed sticker bonds with the aluminium lid.

Further, the size of the filtering membrane is 0.22 um.

The utility model has the beneficial effects that: through increase sample filtration membrane on sample placement equipment, simplify the step that the sample treatment shifted, reduce product pollution's risk, and hang the placing of rope convenience sample loading in the sample purification process through plastics, be favorable to increasing the utilization in chromatography cabinet space, the device is simple, and is with low costs for glass equipment, but disposable reduces the pollution.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required to be used in the embodiments of the present invention will be briefly described below.

FIG. 1 is a schematic perspective view of the present invention;

FIG. 2 is an exploded perspective view of the filter connector;

FIG. 3 is an exploded perspective view of the connector assembly;

FIG. 4 is an exploded perspective view of the closure;

FIG. 5 is a schematic perspective view of a loading tube;

in the figure:

1. a sample loading bag; 1a, hanging a rope by plastic; 1b, a sample inlet pipe; 1c, a sample outlet pipe;

2. a sample loading pipe;

3. a filter connector; 3a, sealing covers; 3b, a filter pipe; 3b1, a first bag receiving pipe; 3c, a filtration membrane;

5. a connecting assembly; 5a, a second bag connecting pipe; 5b, a first connecting pipe; 5b1, a first sealed tube; 5b2, a first arc-shaped snap ring;

6. sealing the cover opening; 6a, a rubber pad; 6b, a second connecting pipe; 6b1, second sealed tube; 6b2, a second arc-shaped snap ring; 6b3, a third arc-shaped snap ring; 6c, an aluminum cover; 6d, sealing the paster.

Detailed Description

The technical scheme of the utility model is further explained by the specific implementation mode in combination with the attached drawings.

Wherein the showings are for the purpose of illustration only and are shown by way of illustration only and not in actual form, and are not to be construed as limiting the present patent; to better illustrate the embodiments of the present invention, some components of the drawings may be omitted, enlarged or reduced, and do not represent the size of an actual product.

Referring to fig. 1 to 5, a protein purification sample loading bag comprises a sample loading bag 1, a sample loading tube 2, a filter connector 3, a connecting assembly 5 and a sealing cover opening 6, wherein a sample outlet tube 1c and a sample inlet tube 1b are arranged at the bottom of the sample loading bag 1, the filter connector 3 is fixedly installed on the sample inlet tube 1b, the connecting assembly 5 is fixedly installed on the sample outlet tube 1c, the top of the sample loading tube 2 is connected with the bottom of the connecting assembly 5, the sealing cover opening 6 is fixedly installed at the bottom of the sample loading tube 2, a flow restrictor for controlling the flow of the sample loading tube 2 is fixedly installed on the sample loading tube 2, a plastic hanging rope 1a is arranged at the top of the sample loading bag 1, the flow restrictor is used for opening, closing and controlling the flow, and the effect is similar to that of a micro peristaltic pump, preferably a micro peristaltic pump.

The filtering connector 3 comprises a sealing cover 3a, a filtering pipe 3b and a filtering film 3c, a first bag connecting pipe 3b1 is arranged at the top of the filtering pipe 3b, the first bag connecting pipe 3b1 is inserted into the sampling pipe 1b, the outer edge of the first bag connecting pipe 3b1 is hermetically connected with the inner edge of the sampling pipe 1b, the filtering film 3c is fixedly installed at the top of the inner edge of the filtering pipe 3b, an external thread is arranged at the bottom of the outer edge of the filtering pipe 3b, an internal thread connected with the external thread is arranged on the inner edge of the sealing cover 3a, and the sealing cover 3a is meshed with the filtering pipe 3 b. When needing to add albumen in the bag of getting a sample 1, at first unscrew sealed lid 3a through the workman, add equipment connection with albumen with filter tube 3b again for albumen is added equipment and is added albumen in the bag of getting a sample 1 through filter tube 3b, and filters albumen through filtration membrane 3c, and through increasing filtration membrane, simple and convenient sample treatment shifts the step, reduces the risk of product pollution.

The connecting assembly 5 comprises a second bag connecting pipe 5a and a first connecting pipe 5b, the top of the second bag connecting pipe 5a is inserted into the sample outlet pipe 1c, the outer edge of the second bag connecting pipe 5a is connected with the inner edge of the sample outlet pipe 1c in a sealing mode, the outer edge of the bottom of the second bag connecting pipe 5a is provided with an external thread, the inner edge of the top of the first connecting pipe 5b is provided with an internal thread connected with the external thread of the second bag connecting pipe 5a, the first connecting pipe 5b is connected with the second bag connecting pipe 5a in a meshing mode, the bottom of the first connecting pipe 5b is provided with a first sealing pipe 5b1, the first sealing pipe 5b1 is inserted into the sample outlet pipe 2, and the outer edge of the first sealing pipe 5b1 is provided with a plurality of first arc-shaped clamping rings 5b2 which are tightly sealed and clamped with the top of the sample outlet pipe 2. Establish on first sealed tube 5b1 through the top connecting pipe card with sample tube 2, rethread first arc snap ring 5b2 struts the connecting pipe, and then accomplishes first connecting pipe 5b and sample tube 2's sealing connection for on the albumen that flows out from sample tube 1c flows into second bag pipe 5a along sample tube 1c, enters into sample tube 2 again through first connecting pipe 5 b.

Sealing cover mouth 6 includes rubber pad 6a, second connecting pipe 6b and aluminium lid 6c, the top of second connecting pipe 6b is provided with second sealed tube 6b1, second sealed tube 6b1 inserts and establishes in last appearance pipe 2, and be provided with on the outer fringe of second sealed tube 6b1 with the sealed chucking a plurality of second arc snap ring 6b2 in last appearance pipe 2 bottom, be provided with third arc snap ring 6b3 on the outer fringe of second connecting pipe 6b, rubber pad 6a inserts and establishes on the inner edge of second connecting pipe 6b, aluminium lid 6c is fixed to be set up on the outer fringe of second connecting pipe 6b, the circular breach that supplies the needle tubing male is seted up to the bottom of aluminium lid 6 c. When the albumen in the sample loading bag 1 needs to be loaded, a worker inserts the needle tube into the second sealing tube 6b1 through the aluminum cap 6c and the rubber pad 6a so that the albumen flows out along the needle tube, and also removes the aluminum cap 6c and the rubber pad 6a and connects the second connecting tube 6b with the purifying machine.

The circular breach of the bottom of aluminium lid 6c is provided with seal sticker 6d, and seal sticker 6d bonds with aluminium lid 6 c. Seal up the circular breach of aluminium lid 6c through seal sticker 6d, avoid gluing a large amount of dust and bacterium on the rubber pad 6 a.

The filtration membrane 3b has a size of 0.22 um.

The working principle is as follows: when protein needs to be added into the sample loading bag 1, firstly, a worker unscrews the sealing cover 3a, and then the filtering pipe 3b is connected with protein adding equipment, so that the protein adding equipment adds the protein into the sample loading bag 1 through the filtering pipe 3b, the protein is filtered through the filtering film 3c, and by adding the filtering film, the step of sample treatment and transfer is simplified, and the risk of product pollution is reduced;

when needs go up the appearance, dismantle aluminium lid 6c and rubber pad 6a down through the workman, be connected second connecting pipe 6b and purification machine, go up the albumen in the appearance bag 1 and will flow out along going out appearance pipe 1c, go out the albumen that appearance pipe 1c flows out and flow into on second bag pipe 5a along going out appearance pipe 1c, enter into in the appearance pipe 2 through first connecting pipe 5b again, the albumen that enters into in the appearance pipe 2 will pass through second sealed tube 6b1 and second connecting pipe 6b, enter into in the purification machine.

Claims (6)

1. The utility model provides a bag of appearance in protein purification, a serial communication port, including bag of appearance (1), go up appearance pipe (2), filter connector (3), coupling assembling (5) and sealed gaikou (6), the bottom of going up appearance bag (1) is provided with out appearance pipe (1 c) and advances appearance pipe (1 b), filter connector (3) fixed mounting is on advancing appearance pipe (1 b), coupling assembling (5) fixed mounting is on going out appearance pipe (1 c), the top of going up appearance pipe (2) is connected with the bottom of coupling assembling (5), sealed gaikou (6) fixed mounting is in the bottom of last appearance pipe (2), the top of bag of going up appearance (1) is provided with plastics string rope (1 a).

2. The protein purification sample loading bag according to claim 1, wherein the filter connector (3) comprises a sealing cover (3 a), a filter tube (3 b) and a filter membrane (3 c), a first bag receiving tube (3 b 1) is arranged at the top of the filter tube (3 b), the first bag receiving tube (3 b 1) is inserted into the sample feeding tube (1 b), the outer edge of the first bag receiving tube (3 b 1) is hermetically connected with the inner edge of the sample feeding tube (1 b), the filter membrane (3 c) is fixedly installed at the top of the inner edge of the filter tube (3 b), an external thread is arranged at the bottom of the outer edge of the filter tube (3 b), an internal thread connected with the external thread is arranged on the inner edge of the sealing cover (3 a), and the sealing cover (3 a) is connected with the filter tube (3 b) in a meshing manner.

3. A protein purification loading bag according to claim 2, wherein the connecting assembly (5) comprises a second bag connecting tube (5 a) and a first connecting tube (5 b), the top of the second bag connecting tube (5 a) is inserted into the sample outlet tube (1 c), the outer edge of the second bag connecting pipe (5 a) is hermetically connected with the inner edge of the sample outlet pipe (1 c), the outer edge of the bottom of the second bag connecting pipe (5 a) is provided with an external thread, the inner edge of the top of the first connecting pipe (5 b) is provided with an internal thread connected with the external thread of the second bag connecting pipe (5 a), the first connecting pipe (5 b) is meshed with the second bag connecting pipe (5 a), the bottom of the first connecting pipe (5 b) is provided with a first sealing pipe (5 b 1), the first sealing pipe (5 b 1) is inserted into the sample outlet pipe (2), and the outer edge of the first sealing tube (5 b 1) is provided with a plurality of first arc-shaped clamping rings (5 b 2) which are tightly clamped with the top of the sample loading tube (2).

4. The protein purification sample loading bag according to claim 3, wherein the sealing cover opening (6) comprises a rubber pad (6 a), a second connecting pipe (6 b) and an aluminum cover (6 c), a second sealing pipe (6 b 1) is arranged at the top of the second connecting pipe (6 b), the second sealing pipe (6 b 1) is inserted into the sample loading pipe (2), a plurality of second arc-shaped clamping rings (6 b 2) which are tightly sealed and clamped with the bottom of the sample loading pipe (2) are arranged on the outer edge of the second sealing pipe (6 b 1), a third arc-shaped clamping ring (6 b 3) is arranged on the outer edge of the second connecting pipe (6 b), the rubber pad (6 a) is inserted into the inner edge of the second connecting pipe (6 b), the aluminum cover (6 c) is fixedly arranged on the outer edge of the second connecting pipe (6 b), and a circular notch for inserting is formed in the bottom of the aluminum cover (6 c).

5. The protein purification sample loading bag according to claim 4, wherein the round notch at the bottom of the aluminum cover (6 c) is provided with a seal sticker (6 d), and the seal sticker (6 d) is adhered with the aluminum cover (6 c).

6. A protein purification loading bag according to claim 5, wherein the filtration membrane (3 c) has a size of 0.22 um.

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