CN215440398U

CN215440398U - Draw separator for biological cell fast

Info

Publication number: CN215440398U
Application number: CN202121344858.1U
Authority: CN
Inventors: 温建勋; 徐亚楠; 牛燕; 王忆霄; 白雪
Original assignee: Inner Mongolia Medical University
Current assignee: Inner Mongolia Medical University
Priority date: 2021-06-17
Filing date: 2021-06-17
Publication date: 2022-01-07
Anticipated expiration: 2031-06-17

Abstract

The utility model discloses a separation device for rapidly extracting biological cells, which comprises a base and a separation bin; the separation bin comprises a bin body and a bin cover; the middle part of the peripheral wall of the bin body is fixedly connected with the base through a bracket; one side of the bin body is rotationally connected with the bin cover through a rotating shaft, and the other side of the bin body is in buckling connection with the bin cover through a buckling component; the bin cover is provided with at least two spraying ports, and a sprayer is fixedly arranged below the spraying ports; the sprayer is communicated with the water tank through a water pump; the middle part of the bin cover is also provided with a rotating separation component; the bin cover is also provided with a liquid inlet; a filter screen is arranged in the middle of the bin body; the filter screen divides the bin body into a separation area and a discharge area. The extraction rate of the device is obviously improved compared with the traditional process, the optimal extraction rate can be obtained, the extraction time is greatly shortened compared with the traditional method, the extraction solution has less impurities, the effective components are easy to separate and purify, the operation cost of the extraction process is low, the comprehensive economic benefit is obvious, the operation is simple and easy, and the equipment maintenance is convenient.

Description

Draw separator for biological cell fast

Technical Field

The utility model relates to the technical field of cell separation, in particular to a separation device for rapidly extracting biological cells.

Background

Biotechnology means that people use modern life science as a basis, combine with the scientific principles of other basic sciences, adopt advanced scientific and technological means, reform organisms or process biological raw materials according to the design in advance, produce the required products or achieve a certain purpose for human beings; the biotechnology is a technology for processing material raw materials by using microorganisms, animals and plants to provide products to serve society, and mainly comprises fermentation technology and modern biotechnology, so that the biotechnology is a new and comprehensive discipline, and the modern biotechnology is a multi-discipline technology such as genetic engineering, molecular biology, biochemistry, genetics, cell biology, embryology, immunology, organic chemistry, inorganic chemistry, physical chemistry, physics, informatics, computer science and the like, can be used for researching the rules of life activities, providing products to serve society and the like, in the biotechnology, the operation steps of biological cell separation are often carried out to facilitate diversified observation of cells, a biological cell separation device is generally used in the biological cell separation process, but the existing biological cell separation device cannot better carry out the cell separation in a test tube in the operation process The method has the advantages of high efficiency and high speed, and the traditional cell separation method usually adopts a centrifugal method, so that the extraction time is long and the working efficiency is low.

Therefore, it is necessary to design a separation apparatus for rapidly extracting biological cells, which can optimize the separation and extraction rate of cells and shorten the extraction time.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a separation device for rapidly extracting biological cells, which is used for solving the problems in the prior art, and can realize the optimization of the separation and extraction rate of the cells and the shortening of the extraction time.

In order to achieve the purpose, the utility model provides the following scheme: the utility model provides a separation device for rapidly extracting biological cells, which comprises: a base and a separation bin;

the separation bin comprises a bin body and a bin cover; the middle part of the peripheral wall of the bin body is fixedly connected with the base through a bracket; one side of the bin body is rotationally connected with the bin cover through a rotating shaft, and the other side of the bin body is in buckling connection with the bin cover through a buckling component; the bin cover is provided with at least two spraying ports, and a sprayer is fixedly arranged below the spraying ports; the sprayer is communicated with the water tank through a water pump; the middle part of the bin cover is also provided with a rotating separation component; the bin cover is also provided with a liquid inlet;

a filter screen is arranged in the middle of the bin body; the filter screen divides the bin body into a separation area and a discharge area.

The rotating separation assembly comprises a driving motor, a rotating rod and an ultrasonic generator; the driving motor is arranged in the middle of the top surface of the bin cover; the output of driving motor with the one end fixed connection of dwang, the dwang vertical set up in the disengagement zone, just the dwang other end with supersonic generator fixed connection.

The rotating shaft is provided with a first blade and a second blade which are matched with each other; the first blade and the second blade rotate relatively through a rotating shaft; the first blades are fixedly arranged on the side wall of the bin cover, and the second blades are fixedly arranged on the side wall of the top of the bin body.

The top surface of the bin cover is also fixedly provided with a lifting handle.

The bottom of the discharging area is of a funnel-shaped structure, and the center of the bottom surface of the discharging area is provided with a discharging hole; the discharge port is communicated with a transfer disk through a communicating pipe; the unloading disc is respectively communicated with the waste liquid tank, the nuclear cell liquid tank and the separation liquid tank through pipelines.

The waste liquid tank, the nuclear cell liquid tank and the separating liquid tank are all arranged on the top surface of the base.

Electromagnetic valves are arranged on the communication pipelines of the waste liquid tank, the nuclear cell liquid tank and the separation liquid tank; the base is also provided with a control main board; each electromagnetic valve is electrically connected with the control main board.

The buckle assembly comprises a buckle turnover plate and a buckle seat; one end of the buckle turnover plate is fixedly arranged on the side wall of the bin cover, and the other end of the buckle turnover plate is clamped in the buckle seat; the buckle seat is fixedly arranged on the side wall of the top of the bin body; the buckle turnover plate and the buckle seat respectively correspond to the first blade and the second blade in position.

The utility model discloses the following technical effects: the extraction rate of the device is obviously improved compared with the traditional process, the optimal extraction rate can be obtained, the extraction time is greatly shortened compared with the traditional method, the extraction solution has less impurities, the effective components are easy to separate and purify, the operation cost of the extraction process is low, the comprehensive economic benefit is obvious, the operation is simple and easy, and the equipment maintenance is convenient.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without inventive exercise.

FIG. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a schematic view of the structure of the lid of the present invention;

the device comprises a base, a bin body, a bin cover, a support, a rotating shaft, a sprayer, a water tank, a filter screen, a communicating pipe, a transfer plate, a waste liquid tank, a nuclear cell liquid tank, a separation liquid tank, a driving motor, a rotating rod, a supersonic generator, a liquid inlet, a first blade, a second blade, a lifting handle, a clamping turnover plate and a clamping seat, wherein the base is 1-the base, the bin body is 2-the bin body, the bin cover is 3-the bin cover, the support is 4-the rotating shaft is 5-the rotating shaft, the spraying device is 6-the water tank, the filter screen is 8-the communicating pipe, the transfer plate is 10-the waste liquid tank, the nuclear cell liquid tank is 12-the nuclear cell liquid tank, the separation liquid tank is 13-the driving motor, the rotating rod is 15-the ultrasonic generator, the liquid inlet is 17-the first blade, the second blade is 19-the lifting handle, the clamping turnover plate is 21-the clamping turnover plate, and the clamping seat is 22-the clamping seat.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.

The utility model provides a separation device for rapidly extracting biological cells, which comprises: a base 1 and a separation bin;

the separation bin comprises a bin body 2 and a bin cover 3; the middle part of the circumferential wall of the bin body 2 is fixedly connected with the base 1 through a bracket 4; one side of the bin body 2 is rotationally connected with the bin cover 3 through a rotating shaft 5, and the other side of the bin body is connected with the bin cover in a buckling mode through a buckling component; the bin cover 3 is provided with at least two spraying ports, and a sprayer 6 is fixedly arranged below the spraying ports; the sprayer 6 is communicated with the water tank 7 through a water pump; the middle part of the bin cover 3 is also provided with a rotating separation component; the bin cover 3 is also provided with a liquid inlet 17;

a filter screen 8 is arranged in the middle of the bin body 2; the filter screen 8 divides the bin body 2 into a separation area and a discharge area.

In one embodiment of the present invention, the shower ports are arranged symmetrically with respect to the center of the lid 3; the lifting handle 20 is obliquely arranged relative to the rotating shaft 5; the solution to be separated is added through the inlet 17.

In one embodiment of the utility model, the dissolving water is added into the bin body 2 through the spray opening, so that subsequent extraction is facilitated, the ultrasonic generator in the rotary separation assembly utilizes the cavitation of ultrasonic waves to increase the movement frequency and speed of substance molecules, so that the penetrating power of the solution is increased, the dissolution speed of the extracted components is improved, in addition, the diffusion of the extracted components can be accelerated by the secondary effect of the ultrasonic waves, such as thermal effect, mechanical effect and the like, and the extracted components are fully mixed with the solution, so that the ultrasonic separation assembly is also beneficial to extraction, and the wall breaking or deformation of biological cell tissues is promoted by the unique physical properties of the ultrasonic waves.

The rotating separation component comprises a driving motor 14, a rotating rod 15 and an ultrasonic generator 16; the driving motor 14 is arranged in the middle of the top surface of the bin cover 3; the output end of the driving motor 14 is fixedly connected with one end of a rotating rod 15, the rotating rod 15 is vertically arranged in the separation area, and the other end of the rotating rod 15 is fixedly connected with an ultrasonic generator 16.

Furthermore, two ultrasonic generators 16 can be symmetrically arranged on the outer wall of the bin body 2 and are matched with the internal rotating separation component for use.

In the embodiment, the driving motor 14 can drive the ultrasonic generator 16 to rotate, so that the cell wall can be better crushed, and the effective components can be dissolved; and the power of the ultrasonic generator 16 can be adjusted to realize multi-effect separation.

The rotating shaft 5 is provided with a first blade 18 and a second blade 19 which are matched with each other; the first blade 18 and the second blade 19 rotate relatively through the rotating shaft 5; the first blade 18 is fixedly arranged on the side wall of the bin cover 3, and the second blade 19 is fixedly arranged on the side wall of the top of the bin body 2.

The top surface of the bin cover 3 is also fixedly provided with a lifting handle 20.

The bottom of the discharging area is of a funnel-shaped structure, and the center of the bottom surface of the discharging area is provided with a discharging hole; the discharge port is communicated with a transfer disk 10 through a communicating pipe 9; the transfer tray 10 is respectively communicated with a waste liquid tank 11, a nuclear cell liquid tank 12 and a separation liquid tank 13 through pipelines.

The waste liquid tank 11, the nuclear cell liquid tank 12 and the separating liquid tank 13 are all arranged on the top surface of the base 1.

Electromagnetic valves are arranged on the communication pipelines of the waste liquid tank 11, the nuclear cell liquid tank 12 and the separation liquid tank 13; the base 1 is also provided with a control main board; each electromagnetic valve is electrically connected with the control mainboard.

In one embodiment of the utility model, the electromagnetic valve is used for controlling the on-off of the communication pipeline; the transfer storage disk 10 and the filter screen 8 are both of a multilayer structure, and different dialysis membranes are arranged between adjacent layers; the waste liquid, the cell nucleuses and the separation liquid are separated in layers and enter the corresponding waste liquid tank 11, the cell nucleuses liquid tank 12 and the separation liquid tank 13.

Preferably, the nuclear cell liquid tank 12 is disposed just below the transfer tray 10, and the waste liquid tank 11 and the separation liquid tank 13 are disposed below both sides of the transfer tray 10.

The buckle assembly comprises a buckle turnover plate 21 and a buckle seat 22; one end of the buckle turnover plate 21 is fixedly arranged on the side wall of the bin cover 3, and the other end is clamped in the buckle seat 22; the buckle seat 22 is fixedly arranged on the side wall of the top of the bin body 2; the catch flap 21 and the catch seat 22 correspond in position to the first blade 18 and the second blade 19, respectively.

In one embodiment of the present invention, the latch assembly and the rotating shaft 5 are separately disposed at two opposite ends, the bin cover 3 is sealed or separated from the bin body 2 by the latch assembly, and when the latch between the latch turnover plate 21 and the latch base 22 is released, the bin cover 3 is rotated around the rotating shaft 5 by lifting the handle 20 upwards, and the bin cover 3 is opened.

The device can promote the cell tissue wall breaking or deformation based on the unique physical characteristics of ultrasonic waves, the extraction rate is obviously improved compared with the traditional process, the optimal extraction rate can be obtained, the extraction time is greatly shortened compared with the traditional method, the ultrasonic extraction is not limited by the polarity and the molecular weight of components, the extraction solution has less impurities, the effective components are easy to separate and purify, the operation cost of the extraction process is low, the comprehensive economic benefit is obvious, the operation is simple and easy, and the equipment maintenance is convenient.

In the description of the present invention, it is to be understood that the terms "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, are merely for convenience of description of the present invention, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.

The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.

Claims

1. A separation device for rapidly extracting biological cells is characterized by comprising: a base (1) and a separation bin;

the separation bin comprises a bin body (2) and a bin cover (3); the middle part of the peripheral wall of the bin body (2) is fixedly connected with the base (1) through a bracket (4); one side of the bin body (2) is rotatably connected with the bin cover (3) through a rotating shaft (5), and the other side of the bin body is connected with the bin cover (3) in a buckling manner through a buckling component; at least two spraying ports are formed in the bin cover (3), and a sprayer (6) is fixedly mounted below the spraying ports; the sprayer (6) is communicated with the water tank (7) through a water pump; the middle part of the bin cover (3) is also provided with a rotating separation component; the bin cover (3) is also provided with a liquid inlet (17);

a filter screen (8) is arranged in the middle of the bin body (2); the filter screen (8) divides the bin body (2) into a separation area and a discharge area.

2. The separation device for rapidly extracting biological cells as claimed in claim 1, wherein: the rotating separation component comprises a driving motor (14), a rotating rod (15) and an ultrasonic generator (16); the driving motor (14) is arranged in the middle of the top surface of the bin cover (3); the output of driving motor (14) with the one end fixed connection of dwang (15), dwang (15) vertical set up in storehouse body (2), just dwang (15) other end with supersonic generator (16) fixed connection.

3. The separation device for rapidly extracting biological cells as claimed in claim 1, wherein: the rotating shaft (5) is provided with a first blade (18) and a second blade (19) which are matched with each other; the first blade (18) and the second blade (19) rotate relatively through the rotating shaft (5); the first blade (18) is fixedly arranged on the side wall of the bin cover (3), and the second blade (19) is fixedly arranged on the side wall of the top of the bin body (2).

4. The separation device for rapidly extracting biological cells as claimed in claim 1, wherein: the top surface of the bin cover (3) is also fixedly provided with a lifting handle (20).

5. The separation device for rapidly extracting biological cells as claimed in claim 1, wherein: the bottom of the discharging area is of a funnel-shaped structure, and the center of the bottom surface of the discharging area is provided with a discharging hole; the discharge hole is communicated with a transfer disc (10) through a communicating pipe (9); the transfer disc (10) is respectively communicated with the waste liquid tank (11), the nuclear cell liquid tank (12) and the separating liquid tank (13) through pipelines.

6. The separation device for rapidly extracting biological cells as claimed in claim 5, wherein: the waste liquid tank (11), the nuclear cell liquid tank (12) and the separating liquid tank (13) are all arranged on the top surface of the base (1).

7. The separation device for rapidly extracting biological cells as claimed in claim 5, wherein: electromagnetic valves are arranged on the communication pipelines of the waste liquid tank (11), the nuclear cell liquid tank (12) and the separation liquid tank (13); the base (1) is also provided with a control main board; each electromagnetic valve is electrically connected with the control main board.

8. The separation device for rapidly extracting biological cells as claimed in claim 3, wherein: the buckle assembly comprises a buckle turnover plate (21) and a buckle seat (22); one end of the buckle turnover plate (21) is fixedly arranged on the side wall of the bin cover (3), and the other end of the buckle turnover plate is clamped in the buckle seat (22); the buckle seat (22) is fixedly arranged on the side wall of the top of the bin body (2); the buckle turnover plate (21) and the buckle seat (22) correspond to the first blade (18) and the second blade (19) respectively in position.