CN215404170U

CN215404170U - High-efficiency DNA extraction plate

Info

Publication number: CN215404170U
Application number: CN202121307073.7U
Authority: CN
Inventors: 冯子宁; 何方豪; 吴桂香; 庄意君
Original assignee: Guangdong Boao Medical Laboratory Co ltd
Current assignee: Guangdong Boao Medical Laboratory Co ltd
Priority date: 2021-06-11
Filing date: 2021-06-11
Publication date: 2022-01-04
Anticipated expiration: 2031-06-11

Abstract

The utility model relates to the field of medical consumables, in particular to a high-efficiency DNA extraction plate, which comprises a body and a plurality of DNA holes arrayed on the body; the DNA hole sequentially comprises an upper end orifice, a transition orifice, a bottom hole body and a hole bottom from top to bottom, the cross section of the upper end orifice is square, the size of the upper end orifice is equal to that of the transition orifice, and the inner diameters of the transition orifice, the bottom hole body and the hole bottom are sequentially reduced to enable the whole DNA hole to be square in upper part and frustum-shaped in bottom. The utility model has simple structure, convenient use, effective wall hanging prevention and high recovery efficiency.

Description

High-efficiency DNA extraction plate

Technical Field

The utility model relates to the technical field of medical consumables, in particular to a high-efficiency DNA extraction plate.

Background

DNA is a carrier of genetic information, is the most important biological information molecule, and is a main object of molecular biology research, and it is a very important prerequisite to obtain high molecular weight and high purity genomic DNA for performing tests such as sequencing, hybridization, gene expression, library construction, etc., and therefore, extraction of genomic DNA is also one of the most important and basic operations in molecular biology testing technology.

In the process of extracting DNA, it is usually necessary to adopt an extraction plate to shake and extract DNA, the extraction plate in the prior art usually has two structures, one is cuboid (fig. 2), and the second is circular arc (fig. 1), and there are the following disadvantages: on the one hand, the bottom of the extraction plate is arc-shaped, DNA is easily hung on the arc-shaped corners, a pipetting gun is not easy to absorb DNA at the bottom of a hole, the recovery rate is about 60-80%, on the second hand, the whole hole of the extraction plate is cuboid, wall hanging is easy to occur, liquid is easily attached to the hole wall, and the DNA extraction efficiency is further low.

SUMMERY OF THE UTILITY MODEL

In order to solve the problems, the utility model provides the high-efficiency DNA extraction plate which has the advantages of simple structure, convenient use, effective wall hanging prevention and high recovery efficiency.

The technical scheme adopted by the utility model is as follows: the high-efficiency DNA extraction plate comprises a body and a plurality of DNA holes arrayed on the body; the DNA hole sequentially comprises an upper end orifice, a transition orifice, a bottom hole body and a hole bottom from top to bottom, the cross section of the upper end orifice is square, the size of the upper end orifice is equal to that of the transition orifice, and the inner diameters of the transition orifice, the bottom hole body and the hole bottom are sequentially reduced to enable the whole DNA hole to be square in upper part and frustum-shaped in bottom.

The technical scheme is further improved in that the inner diameter of the orifice at the upper end is 9mm, the inner diameter of the bottom of the hole is 6mm, the depth of the hole body at the bottom is 10-15mm, and the integral depth of the DNA hole is 20-25 mm.

The technical proposal is further improved in that the depth of the bottom hole body is 12mm, and the integral depth of the DNA hole is 22 mm.

The technical proposal is further improved in that the inner surface of the hole bottom is of a plane structure.

The technical proposal is further improved in that the extraction plate is of an integrally formed structure made of DNA low-adsorption materials.

The technical proposal is further improved in that the size of the upper end orifice of the DNA hole can be various specifications.

The utility model has the beneficial effects that:

1. according to the extraction plate, the DNA hole is of a two-section structure, the upper part is square, the bottom is frustum-shaped, compared with the integrated cuboid structure in the prior art, liquid sequentially passes through the upper-end orifice, the transition orifice and the bottom hole body and finally reaches the bottom of the hole, the inner diameters of the transition orifice, the bottom hole body and the bottom of the hole are sequentially reduced, the liquid slides downwards along the hole wall after being vibrated and finally reaches the bottom of the hole, the phenomenon of DNA wall hanging is effectively avoided, the DNA recovery efficiency is high, the structure is simple, and the use is convenient.

2. The internal diameter in upper end drill way is 9mm, the internal diameter at bottom of the hole is 6mm, make the whole slope form that is of bottom hole body, the liquid gliding of being convenient for, prevent the wall built-up phenomenon, and the internal diameter difference of the two is about 3mm, if the poor undersize of internal diameter, the degree of inclination is little, the liquid downward landing of being not convenient for, if the internal diameter difference is too big, the bottom of the hole is less, the DNA at bottom of the hole is not convenient for the pipetting gun to absorb, be unfavorable for the recovery of DNA, the experiment proves, when the two internal diameter difference is 3mm, the DNA rate of recovery is the highest this moment, can reach more than 95%.

3. The depth of the bottom hole body is 10-15mm, preferably 12mm, about 5mm higher than the prior art, the whole depth of the DNA hole is 20-25mm, preferably 22mm, the height range can effectively prevent DNA solution from splashing outside in the oscillation and uniform mixing process, prevent reagent pollution in different DNA holes, and can ensure that water vapor of DNA eluent in the heating and concentration process can not flow back to the bottom of the hole after being condensed above the hole wall, thereby further improving the DNA recovery efficiency. Meanwhile, the whole depth of the DNA hole is smaller than that of the prior art, so that the operation of a liquid-transfering gun is convenient, and the use is convenient.

4. The internal surface of hole bottom is planar structure, and for prior art's convex structure, the planar structure planarization is good, and the condition of hanging on curved corner can not appear in DNA, and the liquid-transfering gun absorbs the DNA at hole bottom easily, has further improved recovery efficiency.

5. Draw the board and be DNA low adsorption material integrated into one piece structure, when DNA and DNA hole contact, the adsorption efficiency of DNA hole to DNA is lower, the recovery of the DNA of further being convenient for.

6. The size of the upper end orifice of the DNA hole can be various specifications, and the DNA hole can be suitable for the use requirements of different occasions and has wide application range.

Drawings

FIG. 1 is a schematic view of a prior art extraction plate;

FIG. 2 is a schematic view of the structure of an extraction plate according to the prior art;

fig. 3 is a schematic structural view of an extraction plate of the present invention.

Detailed Description

The utility model will be further described with reference to the accompanying drawings.

Fig. 3 is a schematic view of the structure of the extraction plate of the present invention.

The high efficiency DNA extraction plate 100 comprises a body 110, a baffle plate positioned at the edge of the body 110, and a plurality of DNA holes 120 arrayed on the body 110; the DNA hole 120 sequentially comprises an upper end orifice 121, a transition orifice 122, a bottom hole body 123 and a hole bottom 124 from top to bottom, the cross section of the upper end orifice 121 is square, the size of the upper end orifice 121 is equal to that of the transition orifice 122, and the inner diameters of the transition orifice 122, the bottom hole body 123 and the hole bottom 124 are sequentially reduced, so that the whole DNA hole 120 is square at the upper part and frustum-shaped at the bottom.

According to the extraction plate 100, the DNA hole 120 is of a two-section structure, the upper portion is square, the bottom portion is frustum-shaped, compared with an integrated cuboid structure in the prior art, liquid sequentially passes through the upper end orifice 121, the transition orifice 122 and the bottom hole body 123 and finally reaches the hole bottom 124, the inner diameters of the transition orifice 122, the bottom hole body 123 and the hole bottom 124 are sequentially reduced, the liquid slides downwards along the hole wall after being vibrated and finally reaches the hole bottom 124, the phenomenon of DNA wall hanging is effectively avoided, the DNA recovery efficiency is high, the structure is simple, and the use is convenient.

The internal diameter of upper end drill way 121 is 9mm, the internal diameter of hole bottom 124 is 6mm, make bottom hole body 123 wholly be the slope form, the liquid gliding of being convenient for, prevent the wall built-up phenomenon, and the internal diameter difference of the two is about 3mm, if the poor undersize of internal diameter, the tilting degree is little, be not convenient for liquid landing downwards, if the internal diameter difference is too big, hole bottom 124 is less, the liquid-transfering gun of being not convenient for absorbs the DNA of hole bottom 124, be unfavorable for the recovery of DNA, the experiment proves, when the two internal diameter difference is 3mm, the DNA rate of recovery is highest this moment, can reach more than 95%.

The depth of the bottom hole body 123 is 10-15mm, preferably 12mm, the prior art is usually about 7-8mm, about 5mm higher than the prior art, the overall depth of the DNA hole 120 is 20-25mm, preferably 22mm, the height range can effectively prevent DNA solution from splashing in the oscillation and uniform mixing process, prevent reagent pollution in different DNA holes 120, and can ensure that water vapor of DNA eluent in the heating and concentration process cannot flow back to the hole bottom 124 after being condensed above the hole wall, thereby further improving the DNA recovery efficiency. Meanwhile, the whole depth of the DNA hole 120 is smaller than that of the prior art, so that the operation of a liquid-transfering gun is convenient, and the use is convenient.

The internal surface of hole bottom 124 is planar structure, and for the convex structure of prior art, planar structure planarization is good, and the condition of hanging on curved corner can not appear in DNA, and the pipetting gun absorbs the DNA of hole bottom 124 easily, has further improved recovery efficiency.

The extraction plate 100 is an integrally formed structure made of a DNA low-adsorption material, and when DNA contacts the DNA holes 120, the DNA holes 120 have a low adsorption rate on the DNA, thereby further facilitating the recovery of the DNA.

The size of the upper end orifice 121 of the DNA hole 120 can be various specifications, and the DNA hole can be suitable for the use requirements of different occasions and has wide application range.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims

1. High-efficient DNA draws board, its characterized in that: comprises a body and a plurality of DNA holes arrayed on the body; the DNA hole sequentially comprises an upper end orifice, a transition orifice, a bottom hole body and a hole bottom from top to bottom, the cross section of the upper end orifice is square, the size of the upper end orifice is equal to that of the transition orifice, and the inner diameters of the transition orifice, the bottom hole body and the hole bottom are sequentially reduced to enable the whole DNA hole to be square in upper part and frustum-shaped in bottom.

2. The high efficiency DNA extraction plate of claim 1, wherein: the inner diameter of the upper end orifice is 9mm, the inner diameter of the bottom of the hole is 6mm, the depth of the bottom hole body is 10-15mm, and the integral depth of the DNA hole is 20-25 mm.

3. The high efficiency DNA extraction plate of claim 2, wherein: the depth of the bottom hole body is 12mm, and the integral depth of the DNA hole is 22 mm.

4. The high efficiency DNA extraction plate of claim 3, wherein: the inner surface of the bottom of the hole is of a plane structure.

5. The high efficiency DNA extraction plate as set forth in claim 4, wherein: the extraction plate is of an integrally formed structure made of DNA low-adsorption materials.

6. The high efficiency DNA extraction plate of claim 5, wherein: the size of the DNA pore can be of various sizes.