CN215380583U - Plant tissue culture device - Google Patents

Plant tissue culture device Download PDF

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Publication number
CN215380583U
CN215380583U CN202121204344.6U CN202121204344U CN215380583U CN 215380583 U CN215380583 U CN 215380583U CN 202121204344 U CN202121204344 U CN 202121204344U CN 215380583 U CN215380583 U CN 215380583U
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China
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liquid inlet
culture
nutrient solution
plant tissue
tissue culture
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CN202121204344.6U
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Chinese (zh)
Inventor
宋敏丽
田鹏宇
魏爱丽
燕平梅
郝雪峰
李艳晖
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Taiyuan Normal University
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Taiyuan Normal University
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Abstract

A plant tissue culture device comprises a liquid inlet and discharge system, sliding mechanisms and a mounting frame, wherein the mounting frame is provided with a plurality of layers of culture platforms, culture dishes are arranged on mounting holes arranged on the upper surfaces of the culture platforms, the sliding mechanisms are fixed on the lower surfaces of the culture platforms, the liquid inlet and discharge system is mounted on the sliding mechanisms, the sliding mechanisms are used for driving the liquid inlet and discharge system to move, four sliding mechanisms are arranged in parallel, every two sliding mechanisms are arranged in parallel, the two sliding mechanisms arranged in parallel are symmetrical to the two other sliding mechanisms arranged in parallel, the liquid inlet and discharge system comprises two liquid inlet and discharge assemblies, one of the liquid inlet and discharge assemblies used for extracting nutrient solution in the culture dishes is mounted between the two outer hydraulic telescopic rods, the other liquid inlet and discharge assembly used for injecting nutrient solution into the culture dishes is mounted between the two inner hydraulic telescopic rods, the nutrient solution is convenient to replace, the manpower is reduced, and no interference exists between the liquid inlet and discharge assemblies, the culture dish is more stably installed, the sterile environment can be kept, and the tissue culture condition can be conveniently observed.

Description

Plant tissue culture device
Technical Field
The utility model relates to the technical field of plant tissue culture equipment, in particular to a plant tissue culture device.
Background
In the prior art, the tissue culture of plants is carried out in a culture dish or a culture bottle, and in the culture process, nutrient solution needs to be replaced, most of the prior art adopts manual pouring out of the nutrient solution in the culture dish or the culture bottle, and then new nutrient solution is added, so that tissues in the Jia-ang cultivation are easy to pour out together, and the tissues are easy to be polluted by external bacteria; or the plant tissue is directly taken out of the culture dish and transferred to a new culture environment, so that the damage of the culture tissue is easily caused; moreover, the time consumed by manual operation is longer, and the labor is wasted, so that a device capable of automatically replacing the nutrient solution is needed.
SUMMERY OF THE UTILITY MODEL
To the not enough of above-mentioned relevant prior art, this application provides a plant tissue culture device, can change the nutrient solution automatically, has greatly reduced personnel's work, sets up transparent fly leaf, conveniently observes plant tissue culture's the condition, and hydraulic telescoping rod interferes when avoiding into flowing back system and removing, has stronger practicality.
In order to achieve the above object, the present invention employs the following techniques:
a plant tissue culture device comprises a liquid inlet and outlet system, a sliding mechanism and a mounting rack;
the mounting bracket is equipped with multilayer culture platform, culture platform upper surface interval align to grid mounting hole, and the mounting hole is used for installing the culture dish, and culture platform still is equipped with transparent material's fly leaf along length direction, adopts hinged joint between fly leaf and the mounting bracket, is equipped with the tube hole on the fly leaf and is used for passing the auxiliary pipe, and the upper surface of the culture platform of upper story does not set up ann mounting hole, and the lower surface of the culture platform of lower story does not set up into flowing back system and slide mechanism.
Further, the liquid inlet and drainage system comprises two liquid inlet and drainage assemblies, wherein one liquid inlet and drainage assembly is used for extracting nutrient solution in the culture dish, and the other liquid inlet and drainage assembly is used for injecting the nutrient solution into the culture dish; the liquid inlet and outlet assembly comprises a main pipe and an auxiliary pipe, wherein the auxiliary pipe is arranged at one end of the main pipe, liquid nozzles which are uniformly arranged at intervals are arranged in the main pipe along the axis direction, and the liquid nozzles are used for absorbing nutrient solution in a culture dish or injecting the nutrient solution into the culture dish. The main pipe is fixed on the connecting rod, the connecting rod is provided with fixing holes with uniform intervals along the length direction, and the fixing holes are used for installing liquid nozzles.
Further, slide mechanism is fixed in and cultivates the platform lower surface, advance flowing back system mount on slide mechanism, slide mechanism is used for the drive to advance flowing back system and removes, slide mechanism includes the lead screw, the connecting block, the lead screw is fixed in the spreader, the spreader is fixed at cultivates the platform lower surface, spreader one end is equipped with the motor, the motor is used for driving the lead screw rotatory, the motor is fixed in on the support of spreader one end, the connecting block cover is located on the lead screw, the connecting block lower extreme is connected with hydraulic telescoping rod, the hydraulic telescoping rod lower extreme is flat setting, the flat one side of hydraulic telescoping rod is equipped with the through-hole that runs through.
Furthermore, the number of the sliding mechanisms is four, every two sliding mechanisms are arranged side by side, the two sliding mechanisms arranged side by side are symmetrical to the other two sliding mechanisms arranged side by side, one of the liquid inlet and outlet assemblies for extracting nutrient solution in the culture dish is arranged between the two outer hydraulic telescopic rods, and the other liquid inlet and outlet assembly for injecting nutrient solution into the culture dish is arranged between the two inner hydraulic telescopic rods.
Furthermore, the connecting rod is two, and wherein one connecting rod both ends wear to locate respectively in two hydraulic telescoping rod's of inboard through-hole, and wherein another connecting rod both ends wear to locate respectively in two hydraulic telescoping rod's of outside through-hole.
Furthermore, a gasket is arranged in the mounting hole, and the gasket and the auxiliary pipe are made of rubber materials.
The utility model has the beneficial effects that: the liquid inlet and outlet system is driven to move by adopting a sliding mechanism, so that the nutrient solution can be conveniently replaced for each culture dish; the arrangement of the hydraulic telescopic rod prevents interference between the liquid inlet and outlet assemblies; a rubber gasket is arranged between the culture dish and the mounting hole, so that the culture dish is more stably mounted; the installation is transparent fly leaf, can keep sterile environment, also conveniently ann observes the tissue culture situation simultaneously, is convenient for take out the culture dish.
Drawings
The drawings described herein are for illustrative purposes only of selected embodiments and not all possible implementations, and are not intended to limit the scope of the present disclosure.
Fig. 1 is a perspective view of the overall structure of an embodiment of the present application.
Fig. 2 is a schematic view of the mounting position of the hinge.
Fig. 3 is a schematic view of the assembly of the sliding mechanism and the liquid inlet and outlet system.
Fig. 4 is an enlarged schematic view of a portion a of fig. 1.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the following detailed description of the embodiments of the present invention is provided with reference to the accompanying drawings, but the described embodiments of the present invention are a part of the embodiments of the present invention, not all of the embodiments of the present invention.
Examples
As shown in fig. 1 to 4, the present example provides a plant tissue culture apparatus including: the liquid inlet and outlet system 100, the sliding mechanism 200 and the mounting frame 300;
mounting bracket 300 is equipped with multilayer culture platform 306, culture platform 306 upper surface interval align to grid mounting hole 303, mounting hole 303 is used for installing the culture dish, still be equipped with packing ring 304 in the mounting hole 303, packing ring 304 and auxiliary pipe 102 are the rubber material, culture platform 306 still is equipped with the fly leaf 301 of transparent material along length direction, adopt hinge 305 to be connected between fly leaf 301 and the mounting bracket 300, be equipped with tube hole 302 on the fly leaf 301, tube hole 302 is used for passing auxiliary pipe 102, the upper surface of the upper strata culture platform 306 does not set up ann mounting hole 303, the lower surface of the lower floor culture platform 306 does not set up into drainage system 100 and slide mechanism 200.
Specifically, slide mechanism 200 is fixed in and cultivates platform 306 lower surface for the drive advances drainage system 100 and removes, slide mechanism 200 includes lead screw 202, connecting block 205, lead screw 202 is fixed in among the spreader 201, spreader 201 is fixed in and cultivates platform 306 lower surface, spreader 201 one end is equipped with motor 203, motor 203 is used for driving lead screw 202 rotatory, motor 203 is fixed in on the support 204 of spreader 201 one end, connecting block 205 overlaps locates on lead screw 202, connecting block 205 lower extreme is connected with hydraulic telescoping rod 206, hydraulic telescoping rod 206 lower extreme is flat setting, the flat one side of hydraulic telescoping rod 206 is equipped with the through-hole 207 that runs through.
Specifically, the liquid feeding and discharging system 100 is installed on the sliding mechanism 200, the liquid feeding and discharging system 100 includes two liquid feeding and discharging assemblies, the liquid feeding and discharging assemblies include a main pipe 101 and an auxiliary pipe 102, the auxiliary pipe 102 is installed at one end of the main pipe 101, liquid nozzles 104 are uniformly arranged at intervals along the axial direction of the main pipe 101, the liquid nozzles 104 are used for sucking nutrient solution in a culture dish or injecting nutrient solution into the culture dish, the main pipe 101 is fixed on a connecting rod 105, the connecting rod 105 is provided with fixing holes 106 uniformly arranged at intervals along the length direction, the fixing holes 106 are used for installing the liquid nozzles 104, one of the liquid feeding and discharging assemblies for extracting nutrient solution in the culture dish is installed between two outer hydraulic telescopic rods 206, and the other liquid feeding and discharging assembly for injecting nutrient solution into the culture dish is installed between two inner hydraulic telescopic rods 206.
Specifically, there are four sliding mechanisms 200, every two sliding mechanisms 200 are arranged side by side, two sliding mechanisms 200 arranged side by side are symmetrical to the other two sliding mechanisms 200 arranged side by side, there are two connecting rods 105, two ends of one connecting rod 105 respectively penetrate through the through holes 207 of the two inner hydraulic telescopic rods 206, and two ends of the other connecting rod 105 respectively penetrate through the through holes 207 of the two outer hydraulic telescopic rods 206.
Specific embodiments are described below: firstly, two ends of two connecting rods 105 respectively penetrate through holes 207 at the lower ends of hydraulic telescopic rods 206, then a main pipe 101 is fixed on a connecting plate 207, a liquid nozzle 104 penetrates through a fixing hole 106 on the connecting plate 105, the liquid nozzle 104 is used for contacting nutrient solution in a culture dish, the nutrient solution is sucked out or injected into the culture dish, then one end of an auxiliary pipe 102 penetrates through a pipe hole 302 of a movable plate 306, one end of the auxiliary pipe 102 is fixed at one end of the main pipe 101, after the installation is finished, the two outer hydraulic telescopic rods 206 are connected with a liquid inlet and discharge assembly, the outer liquid inlet and discharge assembly is used for sucking out the nutrient solution in the culture dish, the two inner hydraulic telescopic rods 206 are connected with a liquid inlet and discharge assembly, and the inner liquid inlet and discharge assembly is used for injecting new nutrient solution; starting the motor 203 connected with the outer lead screw 202, driving the two outer lead screws 202 to rotate, driving the liquid inlet and outlet assembly for pumping out the nutrient solution to move to the upper part of the culture dish, then starting the hydraulic telescopic rod 206 at the outer side to enable the liquid inlet and outlet assembly to descend until the liquid nozzle contacts the bottom of the culture dish, starting the water pumping device connected with the outer part of the device, sucking out the nutrient solution from the culture dish and storing the nutrient solution in a waste liquid tank, then starting the hydraulic telescopic rod 206 at the outer side to lift the liquid inlet and outlet assembly for injecting the nutrient solution, then continuing to start the motor 203 connected with the outer lead screw 202, repeating the previous operation to pump out the original nutrient solution in each culture dish, starting the motor 203 connected with the inner lead screw 202 after the nutrient solution is pumped out for the first time, enabling the liquid inlet and outlet assembly for injecting the nutrient solution to move to the upper part of the culture dish, and starting the hydraulic telescopic rod 206 connected with the inner lead screw 202, the liquid nozzle 104 connected with the device is lowered for a preset distance, the injection mechanism outside the device is started, new nutrient solution is conveyed to the main pipe 101 through the auxiliary pipe 102 and is injected into the culture dish through the liquid nozzle 104, then the hydraulic telescopic rod 206 at the inner side is started to lift the liquid inlet and outlet assembly injected with the nutrient solution, and after the operation is repeated, the new nutrient solution is injected into each culture dish.
In the embodiment, the liquid inlet and outlet system is driven to move by the sliding mechanism, so that the culture dish can be replaced with the nutrient solution in batches, and meanwhile, the labor is saved; the hydraulic telescopic rod is arranged to enable the liquid inlet and outlet assemblies to be capable of lifting, so that interference among the liquid inlet and outlet assemblies is avoided, and the liquid inlet and outlet assemblies are prevented from splashing outside the culture dish when nutrient solution is injected; a rubber gasket is arranged between the culture dish and the mounting hole, so that the culture dish is more stably mounted; the installation is transparent fly leaf, can keep aseptic environment, also makes things convenient for ann to observe the tissue culture situation, also is convenient for take out the culture dish simultaneously.
The above is only a preferred embodiment of the present invention and is not intended to limit the present invention, and it is apparent that those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.

Claims (10)

1. A plant tissue culture device, comprising: the liquid inlet and outlet system (100), the sliding mechanism (200) and the mounting frame (300);
the mounting rack (300) is provided with a plurality of layers of culture platforms (306), mounting holes (303) are uniformly arranged on the upper surface of each culture platform (306) at intervals, and the mounting holes (303) are used for mounting culture dishes;
the sliding mechanism (200) is fixed on the lower surface of the culture platform (306), the liquid inlet and outlet system (100) is arranged on the sliding mechanism (200), and the sliding mechanism (200) is used for driving the liquid inlet and outlet system (100) to move;
the liquid feeding and discharging system (100) comprises two liquid feeding and discharging assemblies, wherein one liquid feeding and discharging assembly is used for extracting nutrient solution in the culture dish, and the other liquid feeding and discharging assembly is used for injecting the nutrient solution into the culture dish;
the liquid inlet and outlet assembly comprises a main pipe (101) and an auxiliary pipe (102), wherein the auxiliary pipe (102) is arranged at one end of the main pipe (101), liquid nozzles (104) which are uniformly arranged at intervals are arranged on the main pipe (101) along the axis direction, and the liquid nozzles (104) are used for sucking nutrient solution in a culture dish or injecting the nutrient solution into the culture dish.
2. The plant tissue culture device according to claim 1, wherein the main pipe (101) is fixed on a connecting rod (105), the connecting rod (105) is provided with fixing holes (106) at even intervals along the length direction, and the fixing holes (106) are used for installing the liquid nozzles (104).
3. The plant tissue culture device according to claim 2, wherein the sliding mechanism (200) comprises a screw rod (202) and a connecting block (205), the screw rod (202) is fixed in a cross column (201), the cross column (201) is fixed on the lower surface of the culture platform (306), one end of the cross column (201) is provided with a motor (203), the motor (203) is used for driving the screw rod (202) to rotate, the motor (203) is fixed on a bracket (204) at one end of the cross column (201), the connecting block (205) is sleeved on the screw rod (202), the lower end of the connecting block (205) is connected with a hydraulic telescopic rod (206), the lower end of the hydraulic telescopic rod (206) is flat, and a through hole (207) is formed in the flat surface of the hydraulic telescopic rod (206).
4. The plant tissue culture device according to claim 1, wherein the number of the sliding mechanisms (200) is four, and each two of the sliding mechanisms (200) are arranged side by side, and two of the sliding mechanisms (200) arranged side by side are symmetrical to the other two of the sliding mechanisms (200) arranged side by side.
5. The plant tissue culture device according to claim 3, wherein the number of the connecting rods (105) is two, two ends of one connecting rod (105) are respectively inserted into the through holes (207) of the two inner hydraulic telescopic rods (206), and two ends of the other connecting rod (105) are respectively inserted into the through holes (207) of the two outer hydraulic telescopic rods (206).
6. The plant tissue culture device according to claim 1, wherein the culture platform (306) is further provided with a movable plate (301) along the length direction, the movable plate (301) is connected with the mounting frame (300) through a hinge (305), and the movable plate (301) is provided with a pipe hole (302), and the pipe hole (302) is used for penetrating through the secondary pipe (102).
7. The plant tissue culture device according to claim 3, wherein one of the liquid inlet and outlet assemblies for extracting nutrient solution from the culture dish is installed between the two outer hydraulic telescopic rods (206), and the other liquid inlet and outlet assembly for injecting nutrient solution into the culture dish is installed between the two inner hydraulic telescopic rods (206).
8. The plant tissue culture device according to claim 1, wherein the mounting hole (303) is not formed on the upper surface of the uppermost layer of the culture platform (306), and the liquid inlet and outlet system (100) and the sliding mechanism (200) are not formed on the lower surface of the lowermost layer of the culture platform (306).
9. The plant tissue culture device according to claim 1, wherein a gasket (304) is further arranged in the mounting hole (303), and the gasket (304) and the secondary pipe (102) are both made of rubber.
10. The plant tissue culture device according to claim 6, wherein the movable plate (301) is made of transparent material.
CN202121204344.6U 2021-06-01 2021-06-01 Plant tissue culture device Active CN215380583U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202121204344.6U CN215380583U (en) 2021-06-01 2021-06-01 Plant tissue culture device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202121204344.6U CN215380583U (en) 2021-06-01 2021-06-01 Plant tissue culture device

Publications (1)

Publication Number Publication Date
CN215380583U true CN215380583U (en) 2022-01-04

Family

ID=79678188

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202121204344.6U Active CN215380583U (en) 2021-06-01 2021-06-01 Plant tissue culture device

Country Status (1)

Country Link
CN (1) CN215380583U (en)

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