CN215313155U - Centrifugal separation device for extracting particles - Google Patents

Abstract

The utility model discloses a centrifugal separation device for plasmid extraction, relates to the field of plasmid extraction, and aims to solve the problems that the existing centrifugal separation device for plasmid extraction in the prior art is single in internal structure, inconvenient to take out internal solution and complicated in process. The utility model discloses a device for fixing a display screen, including device casing, electronic jar, limiting plate, connecting axle, axle sleeve, device casing, fixed slot, cover, limiting plate, connecting axle and device casing, the rotation barrel is installed to the top of device casing, the inside of device casing is provided with the fixed slot, the cover is installed to the upper end of rotating the barrel, the lower terminal surface of cover is provided with the limiting plate, electronic jar is all installed to the both sides lower extreme of limiting plate, electronic jar both ends respectively with device casing and limiting plate fixed connection, the internal surface of limiting plate is provided with the activity and detains, just the limiting plate is detained through the activity and is rotated barrel fixed connection, and the inside of fixed slot is provided with the connecting block, the connecting axle is installed to the lower extreme of connecting block, the connecting axle passes through the axle sleeve and is rotated with the device casing and is connected, one side of rotating the barrel is provided with the display screen.

Description

Centrifugal separation device for extracting particles

Technical Field

The utility model relates to the technical field of plasmid extraction, in particular to a centrifugal separation device for plasmid extraction.

Background

The plasmid extraction method is to remove RNA, separate plasmid from bacterial genome DNA, remove protein and other impurities, to obtain relatively pure plasmid, the method for extracting plasmid DNA is various, the extraction yield can be divided into micro extraction, medium extraction and large extraction, the extraction from a using instrument can be divided into general extraction and kit method, the specific operation method can be divided into alkaline lysis method, boiling method, toothpick method and the like, various methods have advantages and disadvantages, suitable extraction methods can be adopted according to different experimental purposes, plasmids are mostly double-stranded and circular DNA molecules, and are auxiliary genetic units which are independent of bacterial chromosome and can be copied and inherited, plasmids are the main DNA carriers when molecular biology experimental operation is carried out, and genetic engineering improvement species and the like are carried out.

Closed-loop plasmid DNA chains cannot be separated from each other because the phosphodiester frameworks of the closed-loop plasmid DNA chains have a topological structure wound with each other, when the temperature is reduced, the bases of the closed-loop DNA are positioned at each position to form a supercoiled molecule, and the plasmid DNA can be recovered from supernatant fluid by centrifugally removing denatured chromosomal DNA and protein; therefore, the market urgently needs to develop a centrifugal separation device for extracting the particles to help people to solve the existing problems.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a centrifugal separation device for plasmid extraction, which solves the problems that the existing centrifugal separation device for plasmid extraction in the background art has a single internal structure, is inconvenient to take out internal solution and has complicated processes.

In order to achieve the purpose, the utility model provides the following technical scheme: the utility model provides a plasmid is taken out and is carried and use centrifugal separation device, includes the device casing, the rotation barrel is installed to the top of device casing, the inside of device casing is provided with the fixed slot, the cover is installed to the upper end of rotating the barrel, the lower terminal surface of cover is provided with the limiting plate, electronic jar is all installed to the both sides lower extreme of limiting plate, electronic jar both ends respectively with device casing and limiting plate fixed connection, the internal surface of limiting plate is provided with the activity and detains, just the limiting plate is detained through the activity and is rotated barrel fixed connection, and the inside of fixed slot is provided with the connecting block, the connecting axle is installed to the lower extreme of connecting block, the connecting axle passes through the axle sleeve and is connected with the rotation of device casing, one side of rotating the barrel is provided with the display screen.

Preferably, the limiting plate and the barrel cover are movably connected through a hinge, a handle is installed at the upper end of the barrel cover, and the handle is fixedly connected with the barrel cover.

Preferably, the lower extreme of rotating the barrel installs unable adjustment base, unable adjustment base passes through fixed screw with the rotation barrel and is connected, unable adjustment base card is gone into the connecting block outside and is passed through cross piece block with the connecting block.

Preferably, the servo motor is installed to one side of rotating the barrel, the belt pulley is all installed to servo motor's lower extreme and the lower extreme of connecting block, the belt pulley all through the flange respectively with servo motor and connecting block fixed connection, two install the hold-in range between the belt pulley, two the belt pulley passes through synchronous belt drive and connects.

Preferably, the rotation barrel includes an inner ring cylinder, the inner ring cylinder is located the positive center of the rotation barrel, just be provided with the breach between the inner ring cylinder and the rotation barrel, the inside of inner ring cylinder is provided with draws the box, it is provided with four, four to draw and be provided with the slipmat between the box.

Preferably, the lower ends of the four extraction boxes are respectively provided with a connecting seat, a clamping slot hole is formed in each connecting seat, the upper end of the fixed base is provided with a buckle, and the connecting seats are fixedly connected with the fixed base through the buckles.

Compared with the prior art, the utility model has the beneficial effects that:

1. the utility model discloses a through the setting of rotating the barrel, when placing and detaching solution and draw the box, it fixes with the rotation barrel earlier to detain the activity, can extend electronic jar to can make the rotation barrel rise, not only conveniently maintain the maintenance to the rotation barrel surface, also conveniently operate the solution in the rotation barrel, thereby convenient to use, easy operation detains the cross piece with the below through the activity, be convenient for assemble and dismantle, the practicality is strong, the convenience is good, the process is simple and convenient.

2. The utility model discloses a through drawing the setting of box, the square box that draws is convenient for fix, and can improve through four slipmats that draw between the box and draw the stability between the box to can improve centrifugal separation's stability, be convenient for improve work quality, and through the outside draw-in groove hole of connecting seat, conveniently pass through the buckle with the connecting seat and fix inside unable adjustment base, thereby can conveniently carry out dismouting and fixed, simple structure, convenient to use.

Drawings

FIG. 1 is a front view of the present invention;

FIG. 2 is a cross-sectional view of the present invention;

FIG. 3 is a top view of the rotary cylinder of the present invention;

fig. 4 is an overall front view of the extraction cassette of the present invention.

In the figure: 1. a device housing; 2. rotating the cylinder; 3. a cylinder cover; 4. a handle; 5. an electric cylinder; 6. a fixed base; 7. a display screen; 8. connecting blocks; 9. a connecting shaft; 10. a belt pulley; 11. a synchronous belt; 12. a servo motor; 13. a limiting plate; 14. a notch; 15. a movable buckle; 16. an inner ring cylinder; 17. an extraction box; 18. a non-slip mat; 19. a connecting seat; 20. a slot clamping hole.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.

Referring to fig. 1-4, an embodiment of the present invention is shown: a centrifugal separation device for extracting particles comprises a device shell 1, a rotary cylinder body 2 is installed above the device shell 1, a fixed groove is arranged in the device shell 1, a cylinder cover 3 is installed at the upper end of the rotary cylinder body 2, a limiting plate 13 is arranged on the lower end face of the cylinder cover 3, electric cylinders 5 are installed at the lower ends of two sides of the limiting plate 13, two ends of each electric cylinder 5 are respectively and fixedly connected with the device shell 1 and the limiting plate 13, a movable buckle 15 is arranged on the inner surface of the limiting plate 13, the limiting plate 13 is fixedly connected with the rotary cylinder body 2 through the movable buckle 15, a connecting block 8 is arranged in the fixed groove, a connecting shaft 9 is installed at the lower end of the connecting block 8, the connecting shaft 9 is rotatably connected with the device shell 1 through a shaft sleeve, a display screen 7 is arranged at one side of the rotary cylinder body 2, the electric cylinders 5 can be extended, so that the rotary cylinder body 2 can be lifted, and the maintenance and overhaul of the outer surface of the rotary cylinder body 2 are convenient, the solution in the rotary cylinder body 2 is also convenient to operate, so that the use is convenient, and the operation is simple.

Further, limiting plate 13 passes through hinge swing joint with cover 3, and handle 4 is installed to the upper end of cover 3, handle 4 and cover 3 fixed connection.

Further, the fixing base 6 is installed to the lower extreme that rotates barrel 2, and fixing base 6 passes through fixed screw with rotation barrel 2 to be connected, and 6 card of fixing base goes into connecting block 8 outsidely and passes through cross block with connecting block 8, detains 15 and the cross block of below through the activity, is convenient for assemble and dismantle, and the practicality is strong, and the convenience is good, and the process is simple and convenient.

Further, servo motor 12 is installed to one side of rotating barrel 2, belt pulley 10 is all installed to servo motor 12's lower extreme and connecting block 8's lower extreme, belt pulley 10 all through the flange respectively with servo motor 12 and connecting block 8 fixed connection, install hold-in range 11 between two belt pulleys 10, two belt pulleys 10 pass through hold-in range 11 transmission and connect, servo motor 12 rotates and drives belt pulley 10 and rotate, can make through belt pulley 10 and rotate barrel 2 and rotate.

Further, the rotating cylinder 2 comprises an inner ring 16, the inner ring 16 is located in the center of the rotating cylinder 2, a gap 14 is formed between the inner ring 16 and the rotating cylinder 2, extraction boxes 17 are arranged inside the inner ring 16, four extraction boxes 17 are arranged, anti-slip pads 18 are arranged among the four extraction boxes 17, and the stability among the extraction boxes 17 can be improved through the anti-slip pads 18 among the four extraction boxes 17, so that the stability of centrifugal separation can be improved.

Further, connecting seat 19 is all installed to four lower extremes that draw box 17, and the inside of connecting seat 19 is provided with card slot hole 20, and unable adjustment base 6's upper end is provided with the buckle, and connecting seat 19 passes through buckle and unable adjustment base 6 fixed connection, through the outside draw-in groove hole 20 of connecting seat 19, conveniently fixes connecting seat 19 inside unable adjustment base 6 through the buckle to can conveniently carry out dismouting and fixed, simple structure, convenient to use.

The working principle is as follows: when in use, the solution is placed inside the extraction box 17, the four extraction boxes 17 pass through the clamping groove holes 20 outside the connecting seat 19, the connecting seat 19 is conveniently fixed inside the fixed base 6 through the buckles, so that the disassembly and the fixation can be conveniently carried out, the structure is simple, the use is convenient, the square extraction box 17 is convenient to fix, the stability between the extraction boxes 17 can be improved through the anti-skid pads 18 among the four extraction boxes 17, the stability of centrifugal separation can be improved, the working quality can be improved, the servo motor 12 rotates to drive the belt pulley 10 to rotate, the rotary cylinder 2 can rotate through the belt pulley 10, the centrifugal separation work is carried out, when the solution extraction boxes 17 are placed and disassembled, the movable buckle 15 and the rotary cylinder 2 are firstly fixed, the electric cylinder 5 can be extended, so that the rotary cylinder 2 can be lifted, the maintenance and overhaul of the outer surface of the rotary cylinder 2 are convenient, the solution in the rotary cylinder body 2 is also convenient to operate, so that the use is convenient, the operation is simple, the assembly and the disassembly are convenient through the movable buckle 15 and the cross block below, and the practicability is strong.

It will be evident to those skilled in the art that the utility model is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the utility model being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims (6)

1. A centrifugal separation device for extracting particles, comprising a device housing (1), characterized in that: a rotary cylinder (2) is arranged above the device shell (1), a fixed groove is arranged in the device shell (1), the upper end of the rotary cylinder body (2) is provided with a cylinder cover (3), the lower end surface of the cylinder cover (3) is provided with a limit plate (13), the lower ends of two sides of the limiting plate (13) are respectively provided with an electric cylinder (5), two ends of the electric cylinder (5) are respectively fixedly connected with the device shell (1) and the limiting plate (13), the inner surface of the limiting plate (13) is provided with a movable buckle (15), the limiting plate (13) is fixedly connected with the rotary cylinder body (2) through a movable buckle (15), a connecting block (8) is arranged in the fixing groove, a connecting shaft (9) is arranged at the lower end of the connecting block (8), the connecting shaft (9) is rotatably connected with the device shell (1) through a shaft sleeve, and a display screen (7) is arranged on one side of the rotating cylinder body (2).

2. A centrifugal separator for extracting a plasmid according to claim 1, wherein: limiting plate (13) and cover (3) pass through hinge swing joint, handle (4) are installed to the upper end of cover (3), handle (4) and cover (3) fixed connection.

3. A centrifugal separator for extracting a plasmid according to claim 1, wherein: fixing base (6) are installed to the lower extreme of rotating barrel (2), fixing base (6) pass through fixed screw with rotation barrel (2) and are connected, fixing base (6) card is gone into connecting block (8) outside and is passed through cross piece block with connecting block (8).

4. A centrifugal separator for extracting a plasmid according to claim 1, wherein: rotate one side of barrel (2) and install servo motor (12), belt pulley (10) are all installed to the lower extreme of servo motor (12) and the lower extreme of connecting block (8), belt pulley (10) all through the flange respectively with servo motor (12) and connecting block (8) fixed connection, two install hold-in range (11) between belt pulley (10), two belt pulley (10) are connected through hold-in range (11) transmission.

5. A centrifugal separator for extracting a plasmid according to claim 1, wherein: rotate barrel (2) including interior ring section of thick bamboo (16), interior ring section of thick bamboo (16) are located the positive center of rotating barrel (2), just be provided with breach (14) between interior ring section of thick bamboo (16) and rotation barrel (2), the inside of interior ring section of thick bamboo (16) is provided with draws box (17), it is provided with four, four to draw box (17) it is provided with slipmat (18) to draw between box (17).

6. The centrifugal separator for extracting a plasmid according to claim 5, wherein: four connecting seats (19) are installed at the lower ends of the extracting boxes (17), clamping groove holes (20) are formed in the connecting seats (19), buckles are arranged at the upper ends of the fixing bases (6), and the connecting seats (19) are fixedly connected with the fixing bases (6) through the buckles.

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