CN215302619U - Low-temperature storage device for stem cell culture - Google Patents
Low-temperature storage device for stem cell culture Download PDFInfo
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- CN215302619U CN215302619U CN202121354593.3U CN202121354593U CN215302619U CN 215302619 U CN215302619 U CN 215302619U CN 202121354593 U CN202121354593 U CN 202121354593U CN 215302619 U CN215302619 U CN 215302619U
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- Prior art keywords
- wall
- liquid nitrogen
- vacuum box
- nitrogen cylinder
- stem cell
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- 210000000130 stem cell Anatomy 0.000 title claims abstract description 26
- 238000004113 cell culture Methods 0.000 title claims abstract description 17
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 94
- 239000007788 liquid Substances 0.000 claims abstract description 47
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 47
- 238000007789 sealing Methods 0.000 claims abstract description 20
- 230000007246 mechanism Effects 0.000 claims abstract description 13
- 238000013016 damping Methods 0.000 claims abstract description 6
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 11
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 11
- 241001330002 Bambuseae Species 0.000 claims description 11
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 11
- 239000011425 bamboo Substances 0.000 claims description 11
- 238000005138 cryopreservation Methods 0.000 claims description 9
- 230000000694 effects Effects 0.000 abstract description 8
- 230000000149 penetrating effect Effects 0.000 abstract description 4
- 238000004321 preservation Methods 0.000 abstract description 2
- 238000010521 absorption reaction Methods 0.000 abstract 1
- 230000035939 shock Effects 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 6
- 230000006872 improvement Effects 0.000 description 5
- 230000009471 action Effects 0.000 description 2
- 229920001821 foam rubber Polymers 0.000 description 2
- 108010022894 Euchromatin Proteins 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- GQWNECFJGBQMBO-UHFFFAOYSA-N Molindone hydrochloride Chemical compound Cl.O=C1C=2C(CC)=C(C)NC=2CCC1CN1CCOCC1 GQWNECFJGBQMBO-UHFFFAOYSA-N 0.000 description 1
- 108010017842 Telomerase Proteins 0.000 description 1
- 210000003855 cell nucleus Anatomy 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 210000000632 euchromatin Anatomy 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a low-temperature preservation device for stem cell culture, which comprises a vacuum box, wherein a sponge pad is arranged on the inner wall of the bottom of the vacuum box, the sponge cushion is provided with a liquid nitrogen cylinder, two opposite sides between the upper wall and the lower wall in the liquid nitrogen cylinder are respectively and rotatably connected with a screw rod, the screw rod is provided with moving blocks in a matching and penetrating way, a moving plate is connected between the two moving blocks, the upper wall of the moving plate is uniformly provided with a plurality of placing grooves, a pipeline is communicated with the outer wall of the top of the liquid nitrogen cylinder right above the placing groove, the other end of the pipeline penetrates through the upper wall of the vacuum box and is provided with a first sealing cover, an air inlet pipe is communicated with one side of the outer wall of the top of the liquid nitrogen cylinder, the other end of the air inlet pipe penetrates through the upper wall of the vacuum box and is provided with a sealing cover II, the liquid nitrogen cylinder is provided with a driving mechanism for driving the screw rod to rotate, and the vacuum box is provided with a damping mechanism for slowing down the vibration of the liquid nitrogen cylinder. Compared with the prior art, the utility model has the advantages that: the stem cell test tube is more convenient to store and take, and the shock absorption effect is good.
Description
Technical Field
The utility model relates to the technical field of stem cell culture, in particular to a low-temperature storage device for stem cell culture.
Background
The stem cell is the original cell, it is a kind of primitive undifferentiated cell with multipotentiality and self-replicating ability, it is the primitive cell forming every tissue organ of the mammal, the stem cell has common in shape, usually in the form of round or ellipse, the cell volume is small, the nucleus is relatively large, the cell nucleus is most euchromatin, have higher telomerase activity, in the stem cell culture process, preserve the stem cell through the cryopreservation device.
In many low-temperature storage devices for stem cell culture, test tubes containing stem cells are stored in liquid nitrogen, the existing low-temperature storage devices are inconvenient for storing and taking out the test tubes, and the devices do not have a shock-proof effect when in use and are easy to damage a liquid nitrogen cylinder.
SUMMERY OF THE UTILITY MODEL
In view of the above-mentioned deficiencies of the prior art, the present invention provides a cryopreservation device for stem cell culture.
In order to achieve the purpose of the utility model, the utility model adopts the technical scheme that: a low-temperature preservation device for stem cell culture comprises a vacuum box, wherein a sponge pad is arranged on the inner wall of the bottom of the vacuum box, the sponge cushion is provided with a liquid nitrogen cylinder, two opposite sides between the upper wall and the lower wall in the liquid nitrogen cylinder are respectively and rotatably connected with a screw rod, the screw rod is provided with moving blocks in a matching and penetrating way, a moving plate is connected between the two moving blocks, the upper wall of the moving plate is uniformly provided with a plurality of placing grooves, a pipeline is communicated with the outer wall of the top of the liquid nitrogen cylinder right above the placing groove, the other end of the pipeline penetrates through the upper wall of the vacuum box and is provided with a first sealing cover, an air inlet pipe is communicated with one side of the outer wall of the top of the liquid nitrogen cylinder, the other end of the air inlet pipe penetrates through the upper wall of the vacuum box and is provided with a sealing cover II, the liquid nitrogen cylinder is provided with a driving mechanism for driving the screw rod to rotate, and the vacuum box is provided with a damping mechanism for slowing down the vibration of the liquid nitrogen cylinder.
As an improvement: and universal wheels are respectively arranged at four corners of the outer wall of the bottom of the vacuum box.
As an improvement: and the top of the side wall of one side of the vacuum box is provided with an air outlet, and a third sealing cover is arranged on the air outlet.
As an improvement: the driving mechanism comprises a motor arranged at the center of the inner wall of the bottom of the liquid nitrogen cylinder, a first cylindrical straight gear is connected to the end of the motor shaft, two second cylindrical straight gears are meshed with two opposite sides of the first cylindrical straight gear respectively and are fixedly sleeved on screw rods on two sides respectively.
As an improvement: the damping mechanism comprises slide bars arranged on two opposite sides between the upper wall and the lower wall inside the vacuum box, the slide bars penetrate through the slide blocks in a sliding mode, springs are respectively connected between the upper wall of each slide block and the inner wall of the top of the vacuum box and between the lower wall of each slide block and the inner wall of the bottom of the vacuum box, the slide bars penetrate through the inner rings of the springs, and connecting columns are connected between the slide blocks and the outer side wall of the liquid nitrogen cylinder.
Compared with the prior art, the utility model has the advantages that: open sealed lid one, move up through actuating mechanism drive movable block, it shifts up to drive the movable plate, make movable plate upper wall and the contact of liquid nitrogen section of thick bamboo top inner wall, put into the stem cell test tube through the pipeline, and make stem cell test tube bottom insert the standing groove in, the drive movable block moves down the drive movable plate, make the test tube all be located a liquid nitrogen section of thick bamboo, the test tube access is comparatively convenient, effect and damper's effect through the foam-rubber cushion, can effectively slow down a liquid nitrogen section of thick bamboo sense of shaking, prevent a liquid nitrogen section of thick bamboo damage, add liquid nitrogen in toward a liquid nitrogen section of thick bamboo through the intake pipe, take out the liquid nitrogen attitude with the vacuum box through the gas outlet, can store stem cell low temperature by a vacuum section of thick bamboo, the vacuum box carries out vacuum thermal-insulated, it is better to preserve the effect.
Drawings
FIG. 1 is a schematic front view of a cryopreservation apparatus for stem cell culture according to the present invention;
FIG. 2 is an enlarged view of a portion a of a cryopreservation apparatus for stem cell culture according to the present invention;
reference symbol comparison table:
the device comprises a universal wheel 1, a vacuum box 2, a liquid nitrogen cylinder 3, a straight gear 4, a straight gear 5, a screw mandrel 6, a straight gear 6, a sponge cushion 7, a motor 8, a movable plate 9, a placement groove 10, an air inlet pipe 11, a sealing cover 12, a sealing cover 13, a pipeline 14, a sealing cover I, an air outlet 15, a sealing cover 16, a sliding block 17, a connecting column 18, a sliding rod 19, a spring 20 and a moving block 21.
Detailed Description
The following further describes embodiments of the present invention with reference to the accompanying drawings.
As shown in fig. 1 and 2, a low-temperature storage device for stem cell culture comprises a vacuum box 2, a sponge mat 7 is arranged on the inner wall of the bottom of the vacuum box 2, a liquid nitrogen cylinder 3 is arranged on the sponge mat 7, lead screws 5 are respectively and rotatably connected between the upper wall and the lower wall of the liquid nitrogen cylinder 3, the lead screws 5 are provided with moving blocks 21 in a penetrating manner, a moving plate 9 is arranged between the two moving blocks 21 in a penetrating manner, a plurality of placing grooves 10 are uniformly arranged on the upper wall of the moving plate 9, the outer wall of the top of the liquid nitrogen cylinder 3 right above the placing grooves 10 is communicated with a pipeline 13, the other end of the pipeline 13 penetrates through the upper wall of the vacuum box 2 and is provided with a first sealing cover 14, an air inlet pipe 11 is communicated with one side of the outer wall of the top of the liquid nitrogen cylinder 3, the other end of the air inlet pipe 11 penetrates through the upper wall of the vacuum box 2 and is provided with a second sealing cover 12, and a driving mechanism for driving the lead screws 5 to rotate is arranged on the liquid nitrogen cylinder 3, and the vacuum box 2 is provided with a damping mechanism for slowing down the vibration of the liquid nitrogen cylinder 3.
The four corners of the outer wall of the bottom of the vacuum box 2 are respectively provided with a universal wheel 1.
An air outlet 15 is formed in the top of the side wall of one side of the vacuum box 2, and a third sealing cover 16 is arranged on the air outlet 15.
The driving mechanism comprises a motor 8 arranged at the center of the inner wall of the bottom of the liquid nitrogen cylinder 3, a first cylindrical straight gear 6 is connected to the shaft end of the motor 8, two cylindrical straight gears 4 are respectively meshed with two opposite sides of the first cylindrical straight gear 6 and are respectively fixedly sleeved on screw rods 5 on two sides.
Damping device is including setting up the slide bar 19 of relative both sides between 2 inside upper walls of vacuum box and lower wall, slide bar 19 slides and passes and be equipped with slider 17, be connected respectively between slider 17 upper wall and the 2 top inner walls of vacuum box and between slider 17 lower wall and the 2 bottom inner walls of vacuum box and be equipped with spring 20, slide bar 19 passes spring 20 inner circle, it is equipped with spliced pole 18 to be connected between slider 17 and the 3 lateral walls of liquid nitrogen section of thick bamboo.
When the device is implemented specifically, the device can be moved to a specified position through the action of the universal wheels 1; opening a first sealing cover 14, starting a motor 8 to rotate forwardly to drive a first cylindrical spur gear 6 to rotate forwardly, driving a second cylindrical spur gear 4 to rotate due to the fact that the first cylindrical spur gear 6 is meshed with the second cylindrical spur gear 4, further driving a lead screw 5 to rotate, enabling a moving block 21 to move upwards under the action of internal threaded force of the moving block 21, driving a moving plate 9 to move upwards, stopping the motor 8 when the upper wall of the moving plate 9 is contacted with the inner wall of the top of a liquid nitrogen cylinder 3, putting a dry cell test tube into a pipeline 13, enabling the bottom of the dry cell test tube to be inserted into a placing groove 10, starting the motor 8 to rotate reversely, driving the first cylindrical spur gear 6 to rotate reversely, driving the moving block 21 to move downwards, driving the moving plate 9 to move downwards, enabling the whole test tube to be located in the liquid nitrogen cylinder 3, and closing the first sealing cover 14; opening the second sealing cover 12, introducing liquid nitrogen through the air inlet pipe 11, and then closing the second sealing cover 12, wherein the liquid nitrogen can play a role in storing stem cells at a low temperature; the third sealing cover 16 is opened, the vacuum box 2 is pumped to a vacuum state through the air outlet 15, and the vacuum box 2 can play a role in heat insulation; when the device receives external force reason and takes place the vibration, through the effect of foam-rubber cushion 7, can play first layer bradyseism effect, when liquid nitrogen section of thick bamboo 3 vibrates from top to bottom, can drive slider 17 and slide from top to bottom along slide bar 19 for the spring 20 of upper and lower both sides is flexible, thereby can effectively slow down the sense of vibration of liquid nitrogen section of thick bamboo 3, prevents that liquid nitrogen section of thick bamboo 3 from damaging.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the present invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (5)
1. A cryopreservation device for stem cell culture comprises a vacuum box (2), and is characterized in that: a spongy cushion (7) is arranged on the inner wall of the bottom of the vacuum box (2), a liquid nitrogen cylinder (3) is arranged on the spongy cushion (7), two opposite sides between the upper wall and the lower wall in the liquid nitrogen cylinder (3) are respectively and rotatably connected with a lead screw (5), the lead screw (5) is matched and penetrates through a moving block (21), a moving plate (9) is connected and arranged between the two moving blocks (21), a plurality of placing grooves (10) are uniformly formed in the upper wall of the moving plate (9), a pipeline (13) is communicated with the outer wall of the top of the liquid nitrogen cylinder (3) right above the placing grooves (10), the other end of the pipeline (13) penetrates through the upper wall of the vacuum box (2) and is provided with a first sealing cover (14), an air inlet pipe (11) is communicated with one side of the outer wall of the top of the liquid nitrogen cylinder (3), and the other end of the air inlet pipe (11) penetrates through the upper wall of the vacuum box (2) and is provided with a second sealing cover (12), the liquid nitrogen cylinder (3) is provided with a driving mechanism for driving the screw rod (5) to rotate, and the vacuum box (2) is provided with a damping mechanism for reducing the vibration of the liquid nitrogen cylinder (3).
2. The cryopreservation apparatus for stem cell culture according to claim 1, wherein: the four corners of the outer wall of the bottom of the vacuum box (2) are respectively provided with a universal wheel (1).
3. The cryopreservation apparatus for stem cell culture according to claim 1, wherein: an air outlet (15) is formed in the top of the side wall of one side of the vacuum box (2), and a sealing cover III (16) is arranged on the air outlet (15).
4. The cryopreservation apparatus for stem cell culture according to claim 1, wherein: the driving mechanism comprises a motor (8) arranged at the center of the inner wall of the bottom of the liquid nitrogen cylinder (3), a first cylindrical straight gear (6) is connected to the shaft end of the motor (8), two cylindrical straight gears (4) are respectively meshed with two opposite sides of the first cylindrical straight gear (6) and are respectively fixedly sleeved on screw rods (5) on two sides.
5. The cryopreservation apparatus for stem cell culture according to claim 1, wherein: damper is including setting up slide bar (19) of relative both sides between inside upper wall of vacuum box (2) and lower wall, slide bar (19) slide and pass and be equipped with slider (17), be connected respectively between slider (17) upper wall and vacuum box (2) top inner wall and between slider (17) lower wall and vacuum box (2) bottom inner wall and be equipped with spring (20), spring (20) inner circle is passed in slide bar (19), it is equipped with spliced pole (18) to connect between slider (17) and liquid nitrogen section of thick bamboo (3) lateral wall.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121354593.3U CN215302619U (en) | 2021-06-18 | 2021-06-18 | Low-temperature storage device for stem cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121354593.3U CN215302619U (en) | 2021-06-18 | 2021-06-18 | Low-temperature storage device for stem cell culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215302619U true CN215302619U (en) | 2021-12-28 |
Family
ID=79559022
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202121354593.3U Expired - Fee Related CN215302619U (en) | 2021-06-18 | 2021-06-18 | Low-temperature storage device for stem cell culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215302619U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115140435A (en) * | 2022-06-25 | 2022-10-04 | 广东省人民医院 | Device for preserving umbilical cord blood stem cells |
-
2021
- 2021-06-18 CN CN202121354593.3U patent/CN215302619U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115140435A (en) * | 2022-06-25 | 2022-10-04 | 广东省人民医院 | Device for preserving umbilical cord blood stem cells |
| CN115140435B (en) * | 2022-06-25 | 2023-09-12 | 广东省人民医院 | Device for preserving umbilical cord blood stem cells |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20211228 |