CN215288832U

CN215288832U - Culture medium filling device for stem cell culture

Info

Publication number: CN215288832U
Application number: CN202120939403.8U
Authority: CN
Inventors: 段莉
Original assignee: Shenzhen Second Peoples Hospital
Current assignee: Shenzhen Second Peoples Hospital
Priority date: 2021-05-06
Filing date: 2021-05-06
Publication date: 2021-12-24
Anticipated expiration: 2031-05-06

Abstract

The utility model relates to a cell culture technical field specifically is a culture medium filling device of stem cell culture, including the control box, the control box is bottom open-ended sector plate casing form, is provided with riser and curb plate in the control box, and one side of riser is provided with the curb plate, and the curb plate is fixed on the inner wall of control box, and the top position of riser is provided with the center pin, and the center pin runs through the through-hole of seting up on the riser, and the end fixing of center pin is on the inner wall of control box, is provided with the pressure column between riser and the curb plate, the utility model discloses the structure design has realized the effect of quick even interpolation nutrient solution, realizes stabilizing efficient culture medium filling work, and staff manual control rubber spherical shell can accomplish power pressurization work, and the automatic expansion of filler work afterwards.

Description

Culture medium filling device for stem cell culture

Technical Field

The utility model relates to a cell culture technical field specifically is a culture medium filling device of stem cell culture.

Background

The stem cell culture process in the prior art relates to culture medium filling process, and by regularly adding nutrient solution into a culture dish, the growth of cells in the culture dish is ensured, the actual demand cannot be well met by a common test tube nutrient solution dripping mode, the condition of uneven nutrient solution dripping exists, and the stem cell culture work is influenced.

If the culture medium filling device can be invented, the uniform and efficient nutrient solution adding work can be realized, the problem can be solved, and the culture medium filling device for stem cell culture is provided for the purpose.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a culture medium filling device of stem cell culture to solve the problem that proposes among the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme: a culture medium filling device for stem cell culture comprises a control box, wherein the control box is in a fan-shaped plate shell shape with an opening at the bottom, a vertical plate and a side plate are arranged in the control box, one side of the vertical plate is provided with the side plate, the side plate is fixed on the inner wall of the control box, the top position of the vertical plate is provided with a central shaft, the central shaft penetrates through a through hole formed in the vertical plate, the end part of the central shaft is fixed on the inner wall of the control box, a pressure column is arranged between the vertical plate and the side plate, a square cavity and a thin tube cavity are formed in the vertical plate, the lower end of the square cavity is communicated with a plurality of thin tube cavities which are uniformly distributed, the lower end of the thin tube cavity extends to the outside of the vertical plate, one end of the pressure column extends into the square cavity, a corrugated tube is sleeved on the end part of the pressure column, one side of the control box is fixedly connected with a control plate, one end of the control plate is fixedly connected with a hose, and one end of the hose extends into the control box, the end of the hose extends into the square cavity, the other end of the control panel is fixedly connected with the intercepting block, the control panel is in a square plate shape, a cylindrical groove is formed in a plate body, two control valves are arranged in the cylindrical groove of the control panel, one side of the control panel is provided with a rubber spherical shell, a straight pipe is fixedly connected between the rubber spherical shell and the control panel, one end of the straight pipe extends into the cylindrical groove of the control panel, one side of the central shaft is fixedly connected with the intercepting block, one end of the intercepting block is in contact with a spring piece, and the intercepting block and the spring piece are arranged in the vertical plate.

Preferably, the control valve comprises a sealing cylinder, a sealing ball, a spring and a partition plate, the sealing cylinder and the partition plate are fixed on the inner wall of the cylindrical groove on the control plate, and the sealing ball and the spring are arranged between the sealing cylinder and the partition plate.

Preferably, one end of the spring is contacted with a partition plate, the other end of the spring is contacted with a sealing ball, the partition plate is in a circular plate shape, a plurality of uniformly distributed through holes are annularly arranged on the plate body, and the straight pipe is arranged between the two control valves.

Preferably, the intercepting block and the spring leaf are arranged in an arc-shaped groove formed in the inner wall of the through hole of the vertical plate, the spring leaf is in a wave plate shape, the vertical plate is in a square plate shape, and a plurality of thin tube cavities distributed in parallel are formed in the bottom of the vertical plate.

Preferably, the pressure column is cylindrical, one end of the column body is in a T-shaped disc shape, the column body of the pressure column penetrates through a through hole formed in the vertical plate, and the corrugated pipe is arranged in the vertical plate.

Preferably, the bellows cover is on the cylinder of pressure column, the disk body fixed connection on one end of bellows and the pressure column, the inner chamber wall fixed connection in the other end and the square chamber of bellows.

Compared with the prior art, the beneficial effects of the utility model are that:

1. the utility model realizes the effect of quickly and uniformly adding nutrient solution, realizes the stable and efficient culture medium filling work, can complete the power pressurization work by manually controlling the rubber spherical shell by workers, and then automatically expands the filling work;

2. the utility model discloses a manual rubber spherical shell of pinching repeatedly of staff provides power for the fluid in the control panel, and then during the material pours into square chamber gradually into, and the stable function is powerful, and part structural design is novel.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic diagram of a pressure column.

In the figure: the device comprises a control box 1, a vertical plate 2, a side plate 3, a central shaft 4, a pressure column 5, a square cavity 6, a thin tube cavity 7, a corrugated tube 8, a control plate 9, a hose 10, a control valve 11, a rubber spherical shell 12, a straight tube 13, a blocking block 14, a spring piece 15, a sealing cylinder 16, a sealing ball 17, a spring 18 and a partition plate 19.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the technical scheme in the utility model, all other embodiments that ordinary skilled person in the art obtained under the prerequisite of not making the creative work all belong to the scope of the utility model protection.

Referring to fig. 1 to 2, the present invention provides a technical solution: a culture medium filling device for stem cell culture comprises a control box 1, wherein the control box 1 is in a fan-shaped plate shell shape with an opening at the bottom, a vertical plate 2 and a side plate 3 are arranged in the control box 1, one side of the vertical plate 2 is provided with the side plate 3, the side plate 3 is fixed on the inner wall of the control box 1, a central shaft 4 is arranged at the top position of the vertical plate 2, the central shaft 4 penetrates through a through hole formed in the vertical plate 2, the end part of the central shaft 4 is fixed on the inner wall of the control box 1, a pressure column 5 is arranged between the vertical plate 2 and the side plate 3, a square cavity 6 and a tubule cavity 7 are formed in the vertical plate 2, the lower end of the square cavity 6 is communicated with a plurality of evenly distributed tubule cavities 7, the lower end of the tubule cavity 7 extends to the outside of the vertical plate 2, one end of the pressure column 5 extends into the square cavity 6, the end part of the pressure column 5 is sleeved with a corrugated pipe 8, one side of the control board 9 is fixedly connected with a hose 10 at one end of the control board 9, one end of a hose 10 extends into the control box 1, the end of the hose 10 extends into the square cavity 6, the other end of the control panel 9 is fixedly connected with an interception block 14, the control panel 9 is in a square plate shape, a cylindrical groove is formed in a plate body, two control valves 11 are arranged in the cylindrical groove of the control panel 9, one side of the control panel 9 is provided with a rubber spherical shell 12, a straight pipe 13 is fixedly connected between the rubber spherical shell 12 and the control panel 9, one end of the straight pipe 13 extends into the cylindrical groove of the control panel 9, one side of the central shaft 4 is fixedly connected with the interception block 14, one end of the interception block 14 is contacted with a spring leaf 15, the interception block 14 and the spring leaf 15 are arranged in the vertical plate 2, the position relation of each part is understood by referring to figure 1, the lower end of the control panel 9 is fixedly connected with the hose 10, the upper end of the control panel 9 is connected with an external connection pipe, and the upper end of the external connection pipe is communicated with a storage tank in the prior art, the control box 1 is fixed below the support frame, and a storage box is fixed above the support frame.

The control valve 11 comprises a sealing cylinder 16, a sealing ball 17, a spring 18 and a partition plate 19, wherein the sealing cylinder 16 and the partition plate 19 are fixed on the inner wall of a cylindrical groove on the control plate 9, and the sealing ball 17 and the spring 18 are arranged between the sealing cylinder 16 and the partition plate 19.

One end of the spring 18 is contacted with a partition plate 19, the other end of the spring 18 is contacted with a sealing ball 17, the partition plate 19 is in a circular plate shape, a plurality of uniformly distributed through holes are annularly arranged on the plate body, and the straight pipe 13 is arranged between the two control valves 11.

The intercepting block 14 and the spring piece 15 are arranged in an arc-shaped groove formed in the inner wall of the through hole of the vertical plate 2, the spring piece 15 is in a wave plate shape, the vertical plate 2 is in a square plate shape, and the bottom of the vertical plate 2 is provided with a plurality of thin tube cavities 7 which are distributed in parallel.

The pressure column 5 is cylindrical, one end of the column body is in a T-shaped disc shape, the column body of the pressure column 5 penetrates through a through hole formed in the vertical plate 2, and the corrugated pipe 8 is arranged in the vertical plate 2.

Bellows 8 cover is on the cylinder of pressure column 5, the disk body fixed connection on one end of bellows 8 and the pressure column 5, the inner chamber wall fixed connection of the other end and the square chamber 6 of bellows 8.

The working principle is as follows: the working personnel manually and repeatedly pinch the rubber spherical shell 12, the rubber spherical shell 12 deforms and contracts, nutrient solution between the two control valves 11 is squeezed into the control panel 9, the rubber spherical shell 12 is loosened, the rubber spherical shell 12 elastically recovers to be full, suction is generated in the process, the nutrient solution in an external connecting pipe is sucked between the two control valves 11, the circulation is carried out, the nutrient solution is gradually injected into the square cavity 6, the control valves 11 achieve the effect of one-way flow guide, namely, fluid only can jack the sealing ball 17 through the control valves 11, and further passes through a gap between the sealing ball 17 and the sealing cylinder 16, so that the square cavity 6 is gradually filled with the nutrient solution, the liquid pressure in the square cavity 6 acts on the pressure column 5, the pressure column 5 moves to extend out of the vertical plate 2, one end of the pressure column 5 is propped against the side plate 3, the side plate 3 is fixed, and the vertical plate 2 rotates under the adverse effect, stop afterwards to rubber spherical shell 12 and exert pressure, no longer pour into the nutrient solution into in the square chamber 6 promptly, under the rebound influence of spring leaf 15, reverse rotation takes place for riser 2, riser 2 swings in control box 1 promptly, and riser 2 swing in-process, the nutrient solution in the square chamber 6 outflows gradually through a plurality of thin tube chambeies 7, gathers into the liquid drop low-fall in the bottom of riser 2, place the culture dish in the below of control box 1, nutrient solution evenly spills to fall in the culture dish like this.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A culture medium filling device for stem cell culture comprises a control box (1), and is characterized in that: the control box (1) is in a fan-shaped plate shell shape with an opening at the bottom, a vertical plate (2) and a side plate (3) are arranged in the control box (1), the side plate (3) is arranged on one side of the vertical plate (2), the side plate (3) is fixed on the inner wall of the control box (1), a central shaft (4) is arranged at the top of the vertical plate (2), the central shaft (4) penetrates through a through hole formed in the vertical plate (2), the end part of the central shaft (4) is fixed on the inner wall of the control box (1), a pressure column (5) is arranged between the vertical plate (2) and the side plate (3), a square cavity (6) and a thin cavity (7) are arranged in the vertical plate (2), the lower end of the square cavity (6) is communicated with a plurality of thin tube cavities (7) which are uniformly distributed, the lower end of each thin tube cavity (7) extends to the outside of the vertical plate (2), one end of the pressure column (5) extends into the square cavity (6), the corrugated pipe (8) is sleeved at the end part of the pressure column (5), a control panel (9) is fixedly connected to one side of the control box (1), one end of the control panel (9) is fixedly connected with a hose (10), one end of the hose (10) extends into the control box (1), the end part of the hose (10) extends into the square cavity (6), the other end of the control panel (9) is fixedly connected with a blocking block (14), the control panel (9) is in a square plate shape, a cylindrical groove is formed in a plate body, two control valves (11) are arranged in the cylindrical groove of the control panel (9), a rubber spherical shell (12) is arranged at one side of the control panel (9), a straight pipe (13) is fixedly connected between the rubber spherical shell (12) and the control panel (9), one end of the straight pipe (13) extends into the cylindrical groove of the control panel (9), the blocking block (14) is fixedly connected to one side of the central shaft (4), one end of the interception block (14) is contacted with a spring leaf (15), and the interception block (14) and the spring leaf (15) are arranged in the vertical plate (2).

2. The culture medium filling device for stem cell culture according to claim 1, wherein: the control valve (11) comprises a sealing cylinder (16), a sealing ball (17), a spring (18) and a partition plate (19), the sealing cylinder (16) and the partition plate (19) are fixed on the inner wall of a cylindrical groove in the control plate (9), and the sealing ball (17) and the spring (18) are arranged between the sealing cylinder (16) and the partition plate (19).

3. A medium filling device for stem cell culture according to claim 2, wherein: one end contact of spring (18) has baffle (19), and the other end contact of spring (18) has ball sealer (17), baffle (19) shape is equipped with a plurality of evenly distributed's through-hole for the plectane and plate body are fitted with a contraceptive ring, straight tube (13) set up between two control valve (11).

4. The culture medium filling device for stem cell culture according to claim 1, wherein: the intercepting block (14) and the spring pieces (15) are arranged in arc-shaped grooves formed in the inner wall of the through hole of the vertical plate (2), the spring pieces (15) are wavy plates, the vertical plate (2) is square plate-shaped, and a plurality of thin tube cavities (7) distributed in parallel are formed in the bottom of the vertical plate (2).

5. The culture medium filling device for stem cell culture according to claim 1, wherein: the pressure column (5) is cylindrical, one end of the column body is in a T-shaped disc shape, the column body of the pressure column (5) penetrates through a through hole formed in the vertical plate (2), and the corrugated pipe (8) is arranged in the vertical plate (2).

6. A medium filling device for stem cell culture according to claim 5, characterized in that: bellows (8) cover is on the cylinder of pressure column (5), the disk body fixed connection on one end of bellows (8) and pressure column (5), the inner chamber wall fixed connection of the other end and the square chamber (6) of bellows (8).