CN215288767U

CN215288767U - A vibrate device for exosome draws

Info

Publication number: CN215288767U
Application number: CN202120692299.7U
Authority: CN
Inventors: 徐晓; 段莉; 徐丽梅
Original assignee: Shenzhen Second Peoples Hospital
Current assignee: Shenzhen Second Peoples Hospital
Priority date: 2021-04-06
Filing date: 2021-04-06
Publication date: 2021-12-24
Anticipated expiration: 2031-04-06

Abstract

The utility model discloses an oscillation device for exosome draws, including the base, install a piston section of thick bamboo in the middle of the upper end of base, the piston section of thick bamboo internalization is pegged graft and is had the piston rod, and the upper end threaded connection of piston rod has the screw bolt, and the cover has the go-between on the screw bolt, and the lateral wall vertical welding of go-between has first U type groove, and first U type inslot is connected with the connecting block, and the one end of connecting block is connected with the bracing piece, and anchor clamps are installed perpendicularly to the one end of bracing piece, and the anchor clamps internal fixation has reagent pipe, and beneficial effect is: the utility model provides a vibrating device for exosome draws is first U type groove of six groups of fixedly connected with on the lateral wall of go-between, and the first U type groove of every group all is connected with the bracing piece through the connecting block, just so once only can draw the vibration to six groups of reagent intraductal exosomes, has increased the exosome and has carried out extraction efficiency.

Description

A vibrate device for exosome draws

Technical Field

The utility model relates to an oscillating device field specifically is an oscillating device that is used for exosome to draw.

Background

Exosomes refer to small membrane vesicles (30-150nm) containing complex RNAs and proteins, which today refer specifically to discoidal vesicles with diameters between 40-100 nm. In 1983, exosomes were first found in sheep reticulocytes, which were named "exosomes" by Johnstone in 1987. Many cells secrete exosomes under both normal and pathological conditions. It is mainly from the multivesicular body formed by the invagination of intracellular lysosome particles, and is released into extracellular matrix after the fusion of the outer membrane of the multivesicular body and cell membrane.

All cultured cell types secrete exosomes and exosomes are naturally present in body fluids, including blood, saliva, urine, cerebrospinal fluid and milk. The precise molecular mechanisms involved in their secretion and uptake and their composition, "cargo" and corresponding functions have just begun to be studied. Exosomes are currently regarded as specifically secreted membrane vesicles, involved in intercellular communication, and of increasing interest in exosome research, whether for studying their function or for understanding how they are used in the development of minimally invasive diagnostics

At present the exosome often need vibrate so that better extrasomal of drawing at the in-process that draws, but at the process of vibrating, carry out manual vibration by the experimenter usually, so not only inefficiency, the easy tired ache of arm is unfavorable for raising the efficiency moreover.

Therefore, the utility model provides an oscillating device for exosome draws is used for solving above-mentioned problem.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide an oscillating device for exosome draws to solve the problem that proposes in the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme: an oscillating device for exosome extraction comprises a base, wherein a piston cylinder is installed in the middle of the upper end of the base, a piston rod is movably inserted in the piston cylinder, the upper end of the piston rod is in threaded connection with a threaded bolt, a connecting ring is sleeved on the threaded bolt, a first U-shaped groove is vertically welded on the side wall of the connecting ring, a connecting block is movably connected in the first U-shaped groove through a cylindrical pin, one end of the connecting block is movably connected with a supporting rod through the cylindrical pin, a second U-shaped groove is vertically welded on the lower surface of the supporting rod, a supporting rod is movably connected in the second U-shaped groove through the cylindrical pin and is vertically welded on the upper surface of the base, a clamp is vertically installed at one end of the supporting rod, and a reagent tube is fixed in the clamp;

the lower end of the piston rod is movably connected with a connecting rod, the lower end of the connecting rod is movably installed on a cam rack, one side of the cam rack is fixedly installed on a motor rotating shaft, one side of the cam rack is installed on a support, and the support is vertically installed on the bottom surface of the base.

Preferably, the motor rotating shaft is installed in the motor, the motor is fixedly installed on the supporting block, and the supporting block is fixedly installed on the bottom surface of the base.

Preferably, the threaded bolt is connected with a limiting ring, the radius of the limiting ring is the same as that of the connecting ring, the limiting ring can limit the connecting ring, a threaded hole is formed in the upper end of the piston rod, the piston rod and the threaded bolt are in threaded connection, when different reagent tubes are fixed by the clamp, or the vibration amplitude of the reagent tubes needs to be changed, the height of the connecting ring can be adjusted through the threaded bolt, the piston rod drives the threaded bolt to reciprocate up and down, the vibration amplitude of the connecting ring can be changed, the rotating amplitude of the supporting rod is changed, the vibration amplitude of the exosome can be changed according to different conditions, and therefore the exosome can be better extracted.

Preferably, be fixed with first U type groove on the lateral wall of go-between, first U type groove is total six groups, and first U type groove is circular even equidistance array on the lateral wall of go-between, and first U type groove of every group all is connected with the bracing piece through the connecting block, just so once only can draw six groups of reagent intraductal exosomes and vibrate, has increased the exosome and has carried out extraction efficiency.

Compared with the prior art, the beneficial effects of the utility model are that:

the utility model provides a vibrating device for exosome draws is seted up threaded hole in the upper end of piston rod, piston rod and screw bolt pass through threaded connection, when anchor clamps fix different reagent pipes, or when needing to change the vibration range of reagent pipe, just can adjust the height of go-between through the screw bolt, so the piston rod can change the vibration range of go-between when driving the screw bolt and carry out reciprocating motion from top to bottom, and then changed the range that the bracing piece rotated, can change the vibration range of exosome's of drawing according to the condition of difference like this, so that better exosome is drawn;

the utility model provides a vibrating device for exosome draws is first U type groove of six groups of fixedly connected with on the lateral wall of go-between, and the first U type groove of every group all is connected with the bracing piece through the connecting block, just so once only can draw the vibration to six groups of reagent intraductal exosomes, has increased the exosome and has carried out extraction efficiency.

Drawings

FIG. 1 is a front view of the structure of the present invention;

FIG. 2 is a perspective view of the present invention;

fig. 3 is a detailed view of a part of the structure of the present invention.

In the figure: the device comprises a base 1, a motor rotating shaft 2, a cam frame 3, a support 4, a connecting rod 5, a piston cylinder 6, a piston rod 7, a threaded bolt 8, a connecting ring 9, a limiting ring 10, a first U-shaped groove 11, a connecting block 12, a supporting rod 13, a second U-shaped groove 14, a supporting rod 15, a clamp 16 and a reagent tube 17.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below. The embodiments of the present invention, all other embodiments obtained by a person skilled in the art without creative work, all belong to the protection scope of the present invention.

Referring to fig. 1 to fig. 3, the present invention provides a technical solution: a vibrating device for exosome extraction comprises a base 1, a piston cylinder 6 is installed in the middle of the upper end of the base 1, a piston rod 7 is movably inserted in the piston cylinder 6, a threaded bolt 8 is connected to the upper end of the piston rod 7 in a threaded manner, a connecting ring 9 is sleeved on the threaded bolt 8, a first U-shaped groove 11 is vertically welded on the side wall of the connecting ring 9, a connecting block 12 is movably connected in the first U-shaped groove 11 through a cylindrical pin, a supporting rod 13 is movably connected at one end of the connecting block 12 through the cylindrical pin, a second U-shaped groove 14 is vertically welded on the lower surface of the supporting rod 13, a supporting rod 15 is movably connected in the second U-shaped groove 14 through the cylindrical pin, the supporting rod 15 is vertically welded on the upper surface of the base 1, a clamp 16 is vertically installed at one end of the supporting rod 13, and a reagent tube 17 is fixed in the clamp 16;

the lower end of the piston rod 7 is movably connected with a connecting rod 5, the lower end of the connecting rod 5 is movably installed on the cam rack 3, one side of the cam rack 3 is fixedly installed on the motor rotating shaft 2, one side of the cam rack 3 is installed on the support 4, and the support 4 is vertically installed on the bottom surface of the base 1.

The motor shaft 2 is arranged in the motor, the motor is fixedly arranged on the supporting block, and the supporting block is fixedly arranged on the bottom surface of the base 1.

It has spacing ring 10 to connect on the screw bolt 8, the radius of spacing ring 10 is the same with the radius of go-between 9, spacing ring 10 can be spacing to go-between 9, threaded hole has been seted up to the upper end of piston rod 7, piston rod 7 and screw bolt 8 pass through threaded connection, when the fixed different reagent pipe 17 of anchor clamps 16, perhaps when needing to change the vibration range of reagent pipe 17, just can adjust the height of go-between 9 through screw bolt 8, when piston rod 7 drives screw bolt 8 and carries out up-and-down reciprocating motion like this, just can change the vibration range of go-between 9, and then changed bracing piece 13 pivoted range, can change the drawing vibration range of exosome like this according to the condition of difference, so that better draw exosome.

Be fixed with first U type groove 11 on the lateral wall of go-between 9, first U type groove 11 is total six groups, and first U type groove 11 is circular even equidistance array on the lateral wall of go-between 9, and first U type groove 11 of every group all is connected with bracing piece 13 through connecting block 12, just so once only can draw the vibration to the exosome in six groups of reagent pipes 17, has increased the exosome and has carried out extraction efficiency.

The working principle is as follows: when vibrating the exosome, at first fix the reagent pipe 17 that holds the exosome on anchor clamps 16, then at the starter motor, motor shaft 2 drives cam carrier 3 and rotates, and cam carrier 3's rotation drives piston rod 7 through connecting rod 5 and carries out up-and-down reciprocating motion, and piston rod 7 then can drive bracing piece 13 through threaded bolt 8 and shake from top to bottom, just so can realize the exosome in reagent pipe 17 and vibrate, realizes the extraction of exosome.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. An oscillation device for exosome extraction, comprising a base (1), characterized in that: a piston cylinder (6) is installed in the middle of the upper end of the base (1), a piston rod (7) is movably inserted into the piston cylinder (6), a threaded bolt (8) is connected to the upper end of the piston rod (7) in a threaded manner, a connecting ring (9) is sleeved on the threaded bolt (8), a first U-shaped groove (11) is vertically welded on the side wall of the connecting ring (9), a connecting block (12) is movably connected into the first U-shaped groove (11) through a cylindrical pin, a supporting rod (13) is movably connected to one end of the connecting block (12) through the cylindrical pin, a second U-shaped groove (14) is vertically welded to the lower surface of the supporting rod (13), a supporting rod (15) is movably connected into the second U-shaped groove (14) through the cylindrical pin, the supporting rod (15) is vertically welded to the upper surface of the base (1), a clamp (16) is vertically installed at one end of the supporting rod (13), and a reagent tube (17) is fixed into the clamp (16);

the lower end of the piston rod (7) is movably connected with a connecting rod (5), the lower end of the connecting rod (5) is movably installed on the cam rack (3), one side of the cam rack (3) is fixedly installed on the motor rotating shaft (2), one side of the cam rack (3) is installed on the support (4), and the support (4) is vertically installed on the bottom surface of the base (1).

2. An oscillating device for exosome extraction according to claim 1, characterized in that: the motor rotating shaft (2) is installed in the motor, the motor is fixedly installed on the supporting block, and the supporting block is fixedly installed on the bottom surface of the base (1).

3. An oscillating device for exosome extraction according to claim 1, characterized in that: the threaded bolt (8) is connected with a limiting ring (10), the radius of the limiting ring (10) is the same as that of the connecting ring (9), the upper end of the piston rod (7) is provided with a threaded hole, and the piston rod (7) is in threaded connection with the threaded bolt (8).

4. An oscillating device for exosome extraction according to claim 1, characterized in that: be fixed with first U type groove (11) on the lateral wall of go-between (9), first U type groove (11) have six groups altogether, and first U type groove (11) are circular even equidistance array on the lateral wall of go-between (9), and first U type groove (11) of every group all are connected with bracing piece (13) through connecting block (12).