CN215288722U

CN215288722U - Hybridization furnace for molecular hybridization

Info

Publication number: CN215288722U
Application number: CN202023092197.6U
Authority: CN
Inventors: 旷乐; 李晨; 王晖
Original assignee: Wuhan Laiken Boao Technology Co ltd
Current assignee: Wuhan Laiken Boao Technology Co ltd
Priority date: 2020-12-21
Filing date: 2020-12-21
Publication date: 2021-12-24
Anticipated expiration: 2030-12-21

Abstract

The utility model discloses a hybridization stove that molecular hybridization used, the furnace body (1) of hybridization stove is long 42cm, wide 28cm, high 28cm, furnace body (1) front portion has furnace gate (3) of taking the handle, and the right side is equipped with shift knob (6), accuse temperature panel (7) and speed governing panel (8), and there is furnace chamber (2) inside, and there is shaking table (9) bottom, and there is ventilation fence (5) at the top, and there is temperature regulating device on the right side. The shaking table (9) is provided with a detachable tray (4). The furnace chamber (2) is made of alloy, ceramic and other materials; the furnace door (3) is made of organic glass, alloy and other materials; the tray (4) is made of alloy and other materials. The utility model discloses a hybridization oven is succinct easy-to-use, the function is various, and the range of application is wide, both can satisfy the needs of nucleic acid molecule hybridization experiment, can regard as uses such as constant temperature shaking table and oven again.

Description

Hybridization furnace for molecular hybridization

Technical Field

The utility model relates to a hybridization stove, concretely relates to hybridization stove that molecular hybridization used belongs to molecular biology technical field.

Background

Nucleic acid molecular hybridization is a process of forming stable heteroduplex DNA molecules from complementary nucleotide sequences by base pairing, and includes various modes such as Southern hybridization, Northern hybridization, dot hybridization, etc., and is a detection means commonly used in the field of molecular biology, and is an important research method for identifying the molecular characteristics of various biological samples.

Nucleic acid molecule hybridization needs to be carried out through processes of membrane transfer, probe preparation, prehybridization, hybridization, membrane washing and the like. Wherein, the processes of pre-hybridization, hybridization and membrane washing need to be carried out in a hybridization furnace. This process is long, typically several hours to tens of hours, during which time the hybridization membrane needs to react with a variety of different hybridization solutions in a closed isothermal environment. The common molecular hybridization equipment on the market comprises a molecular hybridization furnace, a hybridization bag, a water bath shaker and the like. The molecular hybridization furnace has large volume, and the hybridization tube used in cooperation is a glass product, is easy to damage and has high price; the hybridization bag is generally a plastic sealing bag which is easy to damage, the temperature rising speed of the water bath shaking table is slow, and the water in the instrument needs to be replaced frequently.

To the above problem, the utility model provides a novel hybridization oven, its characteristics are as follows:

the volume is moderate, the temperature can be quickly raised to the set temperature (30-65 ℃) and the temperature in the furnace chamber (2) is kept constant, so that the temperature in the furnace is stable in the molecular hybridization process; can accommodate four hybridization boxes with the length of 16-21cm, the width of 11-14cm and the height of 5-7cm, and can simultaneously perform hybridization experiments of four standard nylon membranes (15cm multiplied by 10 cm); the hybridization box used in a matching way is easy to obtain, firm and durable; the shaking table (9) can keep a constant rotating speed (0-200rpm) to ensure that the hybridization membrane can continuously react with the homogeneous hybridization solution in the hybridization box.

SUMMERY OF THE UTILITY MODEL

The utility model discloses can provide a hybridization stove that molecular hybridization used.

The utility model is used for hybridization stove of molecular hybridization, furnace body (1) of hybridization stove is long 42cm, wide 28cm, high 28cm, and furnace body (1) front portion has furnace gate (3) of taking the handle, and the right side is equipped with shift knob (6), accuse temperature panel (7) and speed governing panel (8), and inside has furnace chamber (2), bottom has shaking table (9), and there is ventilation fence (5) at the top. The right part of the furnace body (1) is provided with a temperature control device.

The furnace chamber (2) of the utility model has the length of 35cm, the width of 25cm and the height of 18cm, and is made of materials such as alloy, ceramics and the like; the furnace door (3) is 37cm long, 20cm wide and 2cm thick and is made of organic glass, alloy and other materials; the number of the ventilation fences (5) is 2, the length is 10-20cm, and the width is 1-3 cm.

The shaking table (9) is provided with a detachable tray (4), the tray (4) is 30cm long, 22cm wide and 1cm thick and is made of alloy materials.

The utility model has the main advantages that:

1. the volume is moderate. The oven chamber (2) can accommodate 1-4 hybridization boxes with the length of 16-21cm, the width of 11-14cm and the height of 5-7cm, and can simultaneously perform hybridization experiments on 1-4 standard nylon membranes (15cm multiplied by 10 cm).

2. The function is various. The hybridization furnace has accurate temperature control, and the variable temperature range is 30-65 ℃, so the hybridization furnace can be used as an oven and a dark incubator; the rotating speed of the shaking table (9) is stable, and the variable rotating speed range is 0-200rpm, so that the shaking table can be used as a constant-temperature shaking table to meet various experimental requirements.

3. The matched hybridization box is easy to obtain, low in manufacturing cost, firm and durable.

4. The tray (4) can be taken out, thereby providing convenience for experiment operation and being convenient for cleaning.

5. The solution is convenient to replace in the hybridization process. The hybridization film needs to react with various solutions (including pre-hybridization solution, detergent, sealing solution and the like) in sequence in the hybridization process, and the front opening design of the hybridization furnace, the detachable tray (4) and the portable matched hybridization box provide great convenience for replacing the hybridization solution in the experiment.

6. No carrier such as water is added into the furnace, and the trouble of replacing water is avoided.

Drawings

Other features, objects and advantages of the present application will become more apparent upon reading of the following detailed description of non-limiting embodiments thereof, made with reference to the accompanying drawings in which:

FIG. 1 is a schematic perspective view of the outside of a hybridization oven.

FIG. 2 is a schematic perspective view of the interior of the hybridization furnace.

Description of reference numerals:

1: a furnace body; 2: a furnace chamber; 3: a furnace door; 4: a tray; 5: a ventilation fence; 6: a switch button; 7: a temperature control panel; 8: a speed regulation panel; 9: shaking table.

Detailed Description

The present invention will be further described with reference to the accompanying drawings, but the present invention is not limited to the following embodiments.

As shown in the figures 1-2, the utility model discloses a hybridization oven for molecular hybridization, furnace body (1) length 42cm, wide 28cm, high 28cm, furnace body (1) front portion has furnace gate (3) of taking the handle, and the right side is equipped with shift knob (6), accuse temperature panel (7) and speed governing panel (8), and inside has furnace chamber (2), and there is shaking table (9) bottom, and the top has ventilation fence (5). The right part of the furnace body (1) is provided with a temperature control device.

The utility model discloses a set up on shaking table (9) and to dismantle tray (4), tray (4) length 30cm, wide 22cm, thick 1cm are made by alloy material.

The specific principle is as follows:

after the preliminary preparation work of nucleic acid molecule hybridization such as electrophoresis, membrane conversion and the like is finished, an experimenter puts a nylon membrane (with the standard size of 15cm multiplied by 10cm) containing a nucleic acid sample to be detected into a hybridization box filled with a proper amount of pre-hybridization liquid, then opens a furnace door (3), puts the hybridization box into a furnace chamber (2) of the hybridization furnace, puts the hybridization box on a tray (4), and closes the furnace door (3). The oven chamber (2) can accommodate four hybridization boxes at most and simultaneously carry out hybridization experiments of four standard hybridization membranes. Next, the switch button (6) of the hybridization oven is turned on, and the experimenter sets the hybridization temperature (for example, set the hybridization temperature at 42 ℃) through the temperature control panel (7) and sets the rotation speed (for example, 50rpm) of the shaker (9) through the speed control panel (8). After prehybridization for a certain time, opening the furnace door (3), taking out the hybridization box (if a plurality of hybridization boxes are available, the hybridization box can also be taken out together with the tray (4)), pouring out the prehybridization liquid in the box, adding the hybridization liquid, putting the hybridization box (or the hybridization box together with the tray (4)) back to the furnace chamber (2), closing the furnace door (3), and resetting the hybridization temperature and the rotating speed of the shaking table (9) according to the experimental needs. After hybridization for a certain time, the washing solution, the blocking solution and the like required by the experiment can be replaced according to the process until the whole hybridization process is completed. The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, and although the present invention has been disclosed in the above description with reference to the preferred embodiments, it is not intended to limit the present invention, and any person skilled in the art can make modifications or changes to equivalent embodiments without departing from the scope of the present invention.

Claims

1. A hybridization furnace for molecular hybridization is characterized in that a furnace body (1) of the hybridization furnace is 42cm long, 28cm wide and 28cm high; the front part of the furnace body (1) is provided with a furnace door (3) with a handle, and the furnace door (3) is 37cm long, 20cm wide and 2cm thick and is made of organic glass, alloy and other materials; a switch button (6), a temperature control panel (7) and a speed regulation panel (8) are arranged on the right side; a furnace chamber (2) is arranged inside, and the length of the furnace chamber (2) is 35cm, the width is 25cm, and the height is 18 cm; the bottom of the shaking table is provided with a shaking table (9), the shaking table (9) is provided with a detachable tray (4), and the tray (4) is 30cm long, 22cm wide and 1cm thick and is made of alloy and other materials; the top of the ventilation fence (5) is provided with 2 ventilation fences, and each ventilation fence (5) is 10-20cm long and 1-3cm wide.