CN215262745U

CN215262745U - Electrophoresis enrichment device for detecting blood trace neural protein

Info

Publication number: CN215262745U
Application number: CN202121308676.9U
Authority: CN
Inventors: 邓杰
Original assignee: Aidi Biotechnology Beijing Co ltd
Current assignee: Aidi Biotechnology Beijing Co ltd
Priority date: 2021-06-11
Filing date: 2021-06-11
Publication date: 2021-12-21
Anticipated expiration: 2031-06-11

Abstract

The utility model discloses an electrophoresis enrichment device for detecting blood micro-quantity nerve protein, which comprises an electrophoresis box, wherein the top end of the electrophoresis box is fixedly connected with a cover plate, the front left side of the top end of the cover plate is fixedly connected with a positive terminal, the front right side of the top end of the cover plate is fixedly connected with a negative terminal, the left side and the right side of the electrophoresis box are respectively and fixedly connected with a current conducting plate corresponding to the positive terminal and the negative terminal, the middle part of the inner wall of the bottom end of the electrophoresis box is fixedly connected with a gel groove, the front part and the rear middle part of the top end of the gel groove are respectively and fixedly connected with a first electric telescopic rod, the driving ends of the first electric telescopic rods are respectively and fixedly connected with the two ends of an electrophoresis comb, in the utility model, the gel can be fixed, the gel is prevented from shaking caused by bubbles generated during electrophoresis, the effect of enriching the protein by the electrophoresis separation is optimized, and the insertion depth of the gel is easily controlled by the insertion holes of the electrophoresis comb, is worthy of being widely popularized.

Description

Electrophoresis enrichment device for detecting blood trace neural protein

Technical Field

The utility model relates to an electrophoresis separation enrichment protein technical field especially relates to an electrophoresis enrichment device that detects blood trace neural protein.

Background

Electrophoresis is a short-term electrophoresis phenomenon, which refers to a phenomenon that charged particles move towards an electrode with an electric property opposite to that of the charged particles under the action of an electric field, and a technology for separating the charged particles by utilizing different moving speeds in the electric field is called an electrophoresis technology, the electrophoresis technology can be used for analyzing, separating and preparing biomolecules such as amino acids, peptides, proteins, nucleotides and the like, and the basic principle of separating mixed proteins by an electrophoresis method is a method for separating a protein mixture according to different charges of the proteins, namely different acid-base properties.

At present, the device is powered on when the gel electrophoresis method is used for separating and enriching the protein, gas is generated in the power-on process, the generated gas easily makes the gel vibrate, the protein separation condition is possibly not obvious, the enrichment effect is poor, and the electrophoresis comb before electrophoresis is inserted into the gel, the depth of the gel is not well regulated and controlled, and the gel can be possibly penetrated to influence the electrophoresis effect.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the defects existing in the prior art and providing an electrophoresis enrichment device for detecting blood trace neural protein.

In order to achieve the above purpose, the utility model adopts the following technical scheme: an electrophoresis enrichment device for detecting blood micro-nerve protein comprises an electrophoresis box, wherein a cover plate is fixedly connected to the top end of the electrophoresis box, pillars are fixedly connected to the left side and the right side of the rear side of the top end of the cover plate, a positive terminal is fixedly connected to the left side of the front side of the top end of the cover plate, a negative terminal is fixedly connected to the right side of the front side of the top end of the cover plate, a current conducting plate is fixedly connected to the lower portions of the inner walls of the left side and the right side of the electrophoresis box, corresponding to the positive terminal and the negative terminal, a gel groove is fixedly connected to the middle portion of the inner wall of the bottom end of the electrophoresis box, a first electric telescopic rod is fixedly connected to the front portion and the middle portion of the rear portion of the top end of the gel groove, driving ends of the first electric telescopic rods are respectively and fixedly connected to two ends of an electrophoresis comb, a second electric telescopic rod is fixedly connected to the middle portion of the bottom end of the cover plate, and a pressing plate is fixedly connected to the driving end of the second electric telescopic rod, the equal fixedly connected with buffer in clamp plate bottom left and right sides, the equal fixedly connected with preforming in buffer bottom.

As a further description of the above technical solution:

the top ends of the support columns are respectively and fixedly connected to two ends of a power rod, and the middle of the bottom end of the power rod is fixedly connected with a direct-current stabilized power supply.

As a further description of the above technical solution:

the direct current stabilized voltage power supply is electrically connected with the positive terminal and the negative terminal.

As a further description of the above technical solution:

the top end of the first electric telescopic rod is provided with a card comb groove.

As a further description of the above technical solution:

the bottom of the electrophoresis comb is fixedly connected with a plurality of comb teeth.

As a further description of the above technical solution:

the positive terminal, the negative terminal and the current conducting plate are electrically connected.

As a further description of the above technical solution:

the utility model discloses a current conducting plate, including apron top, the equal fixedly connected with of current conducting plate outside top has the electric current, the apron top middle part is provided with a plurality of ventholes, the equal fixedly connected with of current conducting plate outside top protects the piece.

As a further description of the above technical solution:

the bottom in the gel tank is provided with a constant temperature pad.

The utility model discloses following beneficial effect has:

1. the utility model discloses in, at first drive the clamp plate through the electronic telescopic link of second and push down, the clamp plate drives the preforming through the buffer and pushes down and carry out the pressfitting to gel fixed, because the gel material is softer, directly can cause the damage to gel to its pressfitting, so when the preforming contacts gel, slowly adjust second electric telescopic link's speed of pushing down, according to the hardness of different gels, observe the deformation degree of buffer, when the buffer has certain elastic potential energy, enough eliminate the influence that produces the tremor and lead to the fact electrophoresis effect to the bubble to the pressure of gel.

2. The utility model discloses in, drive the electrophoresis comb up-and-down motion through electric telescopic handle and can stably adjust the degree of depth that the electrophoresis comb inserted the gel, avoided the electrophoresis comb to insert the degree of depth influence electrophoresis effect of gel, and prevented that the electrophoresis comb from penetrating through the problem that gel made the unable realization of electrophoresis enrichment protein, the electrophoresis comb of different models is conveniently changed to the card comb groove on the first electric telescopic handle simultaneously, is worth wideling popularize.

Drawings

Fig. 1 is a perspective view of an electrophoresis enrichment device for detecting blood microneuroprotein according to the present invention;

FIG. 2 is a front view of an electrophoresis enrichment device for detecting blood microneuroprotein of the present invention;

fig. 3 is a schematic view of a part of the structure of the electrophoresis enrichment device for detecting blood microneuroprotein of the present invention.

Illustration of the drawings:

1. an electrophoresis cassette; 2. a positive terminal; 3. a cover plate; 4. a pillar; 5. an air outlet; 6. a power supply rod; 7. a DC stabilized power supply; 8. a conductive plate; 9. a gel tank; 10. a constant temperature pad; 11. a first electric telescopic rod; 12. an electrophoresis comb; 13. comb teeth; 14. a card slot; 15. a negative terminal; 16. a buffer; 17. tabletting; 18. pressing a plate; 19. a second electric telescopic rod; 20. an insulating protective sheet.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention; the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance, and furthermore, unless otherwise explicitly stated or limited, the terms "mounted," "connected," and "connected" are to be construed broadly and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Referring to fig. 1-3, the present invention provides an embodiment: an electrophoresis enrichment device for detecting blood micro-nerve protein comprises an electrophoresis box 1, wherein the top end of the electrophoresis box 1 is fixedly connected with a cover plate 3, the left side and the right side of the rear end of the cover plate 3 are fixedly connected with supporting columns 4, the left side and the left side of the front end of the cover plate 3 are fixedly connected with a positive terminal 2, the right side of the front end of the cover plate 3 is fixedly connected with a negative terminal 15, the lower parts of the positive terminal 2 and the negative terminal 15 corresponding to the inner wall of the left side and the right side of the electrophoresis box 1 are fixedly connected with a current conducting plate 8, the middle part of the inner wall of the bottom end of the electrophoresis box 1 is fixedly connected with a gel groove 9, the front part and the middle part of the top end of the gel groove 9 are fixedly connected with a first electric telescopic rod 11, the driving ends of the first electric telescopic rod 11 are respectively and fixedly connected with the two ends of an electrophoresis comb 12, the middle part of the bottom end of the cover plate 3 is fixedly connected with a second electric telescopic rod 19, the driving end of the second electric telescopic rod 19 is fixedly connected with a pressing plate 18, the left side and the right side of the bottom end of the pressing plate 18 are fixedly connected with buffers 16, and the bottom ends of the buffers 16 are fixedly connected with pressing plates 17.

4 tops of pillar difference fixed connection are at the both ends of

power pole

6, 6 bottom middle part fixedly connected with direct current constant voltage power supply 7 of power pole, direct current constant voltage power supply 7 and positive terminal 2, negative terminal 15 is electric connection, 11 tops of first electric telescopic handle all are provided with card comb groove 14, a plurality of broach 13 of electrophoresis comb 12 bottom fixedly connected with, positive terminal 2, be electric connection between negative terminal 15 and the current conducting

plate

8, 3 top middle parts of apron are provided with a plurality of ventholes 5, the equal fixedly connected with insulation protection piece 20 in current conducting plate 8 outside top, gel groove 9 inslot bottom is provided with constant temperature pad 10.

The working principle is as follows: firstly, the pressing plate 18 is driven to press downwards by the second electric telescopic rod 19, the pressing plate 18 drives the pressing plate 17 to press downwards through the buffer 16 and press and fix the gel, the gel material is soft and can be damaged by pressing directly, therefore, when the pressing plate 17 contacts the gel, the pressing speed of the second electric telescopic rod 19 is slowly adjusted, the deformation degree of the buffer 16 is observed according to the hardness of different gels, when the buffer 16 has certain elastic potential energy, the pressure on the gel is enough to eliminate the influence on the electrophoresis effect caused by vibration generated on bubbles, the depth of the electrophoresis comb 12 inserted into the gel can be stably adjusted by driving the electrophoresis comb 12 to move up and down through the first electric telescopic rod 11, the electrophoresis effect is prevented from being influenced by the depth of the electrophoresis comb 12 inserted into the gel, and the problem that the electrophoresis enrichment protein cannot be realized by the electrophoresis comb 12 penetrating the gel is prevented, meanwhile, the comb clamping groove 14 on the first electric telescopic rod 11 is convenient for replacing the electrophoresis combs 12 of different models.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and variations can be made in the embodiments or in part of the technical features of the embodiments without departing from the spirit and the scope of the invention.

Claims

1. The utility model provides an electrophoresis enrichment device of detection blood trace neural protein, includes electrophoresis box (1), its characterized in that: the electrophoresis apparatus comprises an electrophoresis box (1), a cover plate (3) fixedly connected to the top end of the electrophoresis box (1), pillars (4) fixedly connected to the left side and the right side of the rear end of the cover plate (3), a positive terminal (2) fixedly connected to the left side of the front end of the cover plate (3), a negative terminal (15) fixedly connected to the right side of the front end of the cover plate (3), a current conducting plate (8) fixedly connected to the lower portion of the electrophoresis box (1) corresponding to the positive terminal (2) and the negative terminal (15) and corresponding to the inner wall of the left side and the right side of the electrophoresis box (1), a gel tank (9) fixedly connected to the middle portion of the inner wall of the bottom end of the electrophoresis box (1), a first electric telescopic rod (11) fixedly connected to the front portion and the middle portion of the rear portion of the gel tank (9), driving ends of the first electric telescopic rod (11) respectively fixedly connected to the two ends of an electrophoresis comb (12), and a second electric telescopic rod (19) fixedly connected to the middle portion of the bottom end of the cover plate (3), the driving end of the second electric telescopic rod (19) is fixedly connected with a pressing plate (18), the left side and the right side of the bottom end of the pressing plate (18) are fixedly connected with buffers (16), and the bottom end of each buffer (16) is fixedly connected with a pressing sheet (17).

2. The electrophoresis enrichment device for detecting the blood microneuroprotein as claimed in claim 1, wherein: the top ends of the supporting columns (4) are respectively and fixedly connected to the two ends of a power rod (6), and the middle of the bottom end of the power rod (6) is fixedly connected with a direct-current stabilized power supply (7).

3. The electrophoresis enrichment device for detecting the blood microneuroprotein as claimed in claim 2, wherein: the direct-current stabilized power supply (7) is electrically connected with the positive terminal (2) and the negative terminal (15).

4. The electrophoresis enrichment device for detecting the blood microneuroprotein as claimed in claim 1, wherein: the top ends of the first electric telescopic rods (11) are provided with comb clamping grooves (14).

5. The electrophoresis enrichment device for detecting the blood microneuroprotein as claimed in claim 1, wherein: the bottom of the electrophoresis comb (12) is fixedly connected with a plurality of comb teeth (13).

6. The electrophoresis enrichment device for detecting the blood microneuroprotein as claimed in claim 1, wherein: the positive terminal (2), the negative terminal (15) and the conductive plate (8) are electrically connected.

7. The electrophoresis enrichment device for detecting the blood microneuroprotein as claimed in claim 1, wherein: the cover plate is characterized in that a plurality of air outlet holes (5) are formed in the middle of the top end of the cover plate (3), and insulating protection pieces (20) are fixedly connected to the upper portion of the outer side of the conductive plate (8).

8. The electrophoresis enrichment device for detecting the blood microneuroprotein as claimed in claim 1, wherein: the bottom in the gel tank (9) is provided with a constant temperature pad (10).