CN215218663U

CN215218663U - Electrochemical test paper with hematocrit compensation and detection system thereof

Info

Publication number: CN215218663U
Application number: CN202120016164.9U
Authority: CN
Inventors: 胡杰; 牛武; 张海浪; 陆建坤; 傅莹光
Original assignee: Hangzhou Sejoy Electronics & Instruments Co ltd
Current assignee: Zhejiang Shijia Biomedical Co.,Ltd.
Priority date: 2021-01-04
Filing date: 2021-01-04
Publication date: 2021-12-17
Anticipated expiration: 2031-01-04

Abstract

The utility model relates to a check out test set of analyte among the detection blood sample discloses electrochemical test paper and detecting system with hematocrit compensation, include by lower supreme laminating in proper order substrate layer (1), electrode layer (2), enzyme layer (3), siphon guide slot (4) and upper cover plate (5). The substrate layer (1) is made of any one of polyethylene terephthalate, polyvinyl chloride and polypropylene. The electrode layer (2) is a gold electrode layer or a silver carbon electrode layer, and the electrode layer (2) comprises an HCT electrode (21), a counter electrode (22) and a working electrode (23). The upper cover plate (5) is provided with a vent hole. The detection system can correct the hematocrit level of 0-70%, and can calibrate the measured value of the analyte through the relation curve of the measured HCT value and the analyte concentration, and finally display the hematocrit value and the measured value of the analyte in the tester at the same time.

Description

Electrochemical test paper with hematocrit compensation and detection system thereof

Technical Field

The utility model relates to a check out test set of analyte among the detection blood sample especially relates to the electrochemistry test paper and detecting system with hematocrit compensation.

Background

At present, electrochemical sensors are widely used in point-of-care testing (POCT), and have been gradually popularized by professional medical institutions to common home users. One can also conveniently monitor the amount of an analyte (e.g., glucose, uric acid, cholesterol, triglycerides, lactic acid, beta-hydroxybutyrate, etc.) in a blood sample. The accuracy and reliability of the detection result of the electrochemical sensor are also receiving more and more attention.

The electrochemical sensor operates on the principle of converting a proportional concentration of an analyte to be detected into an electrical signal for detection. In the electrode reaction process of the electrochemical sensor, reactants firstly diffuse to the surface of an electrode to react, and the transfer speed of the reactants depends on the effective diffusion coefficient of a solution. The effective diffusion coefficient in blood is mainly influenced by hematocrit, the higher the hematocrit value, the smaller the effective diffusion coefficient, and vice versa. The range of normal hematocrit is about 35% to 55%, while the range of hematocrit for organ failure patients, anemia, blood loss patients, or newborn patients is about 20% to 70%, and such a wide range of hematocrit causes a large error in the electrochemical sensor. Therefore, the development of an electrochemical sensor with a hematocrit correction function can be adapted to the wider demand of people.

For example, the test paper with hematocrit-compensated high-precision uric acid electrochemical test paper with application number 201811122672.4 has single function and can only display uric acid measured values on a detector.

SUMMERY OF THE UTILITY MODEL

The utility model discloses to the shortcoming of electrochemical test paper function singleness, detector among the prior art, provide electrochemical test paper and detecting system with hematocrit compensation.

In order to solve the technical problem, the utility model discloses a following technical scheme can solve:

the electrochemical test paper with the hematocrit compensation comprises a substrate layer, an electrode layer, an enzyme layer, a siphon guide groove and an upper cover plate which are sequentially attached from bottom to top.

Preferably, the substrate layer is made of any one of polyethylene terephthalate, polyvinyl chloride and polypropylene.

Preferably, the electrode layer is a gold electrode layer or a silver carbon electrode layer, and the electrode layer includes an HCT electrode, a counter electrode, and a working electrode.

Preferably, the upper cover plate is provided with a vent hole.

A detection system with electrochemical test paper comprises a micro-control unit and a display screen; the electrode layer comprises an HCT electrode, a counter electrode and a working electrode, the micro control unit is used for applying a single-frequency voltage AC signal without direct current bias between the detection HCT electrode and the counter electrode and calculating a corrected HCT value, and the micro control unit is connected with the display screen and displays the corrected HCT value on the display screen.

Preferably, the micro control unit is used for applying a DC signal of a constant voltage between the counter electrode and the working electrode and calculating an initial analyte concentration value in the blood sample on the electrochemical test strip, and the micro control unit calculates the analyte concentration value by using a pre-input HCT value and an initial analyte concentration value calibration curve and displays the analyte concentration value on the display screen.

The utility model discloses owing to adopted above technical scheme, have apparent technological effect:

1. can correct 0-70% of the hematocrit level while displaying the hematocrit measurement;

2. hematocrit and analyte concentration values may be displayed simultaneously in the test meter.

Drawings

Fig. 1 is a schematic structural diagram of the present invention.

Fig. 2 is a schematic structural view of the electrode layer in fig. 1.

The names of the parts indicated by the numerical references in the above figures are as follows: wherein, 1-substrate layer, 2-electrode layer, 3-enzyme layer, 4-siphon guide groove, 5-upper cover plate, 21-HCT electrode, 22-counter electrode, 23-working electrode.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings 1-2 and examples.

Example 1

The electrochemical test paper with the hematocrit compensation comprises a substrate layer 1, an electrode layer 2, an enzyme layer 3, a siphon guide groove 4 and an upper cover plate 5 which are sequentially attached from bottom to top.

The substrate layer 1 is a substrate layer made of polyethylene terephthalate.

The electrode layer 2 is a silver-carbon electrode layer, the upper layer of the electrode layer 2 adopts a silver layer, and the lower layer adopts a carbon layer. The electrode layer 2 includes an HCT electrode 21, a counter electrode 22, and a working electrode 23.

The upper cover plate 5 is provided with a vent hole.

According to the working principle, blood is sucked into the electrochemical test paper through the siphon guide groove 4, a direct current voltage DC is simultaneously applied to the HCT electrode 21, the counter electrode 22 and the working electrode 23, and the micro control unit respectively collects currents generated on the HCT electrode 21 and the working electrode 23 within a certain time period and serves as initial data for calculating the concentration of the analyte. Then, a single-frequency voltage AC without direct current bias is applied to the HCT electrode 21 and the counter electrode 22, and the micro control unit collects original parameters within a certain time and converts the original parameters into impedance values. The obtained impedance value is calculated through an impedance and hematocrit curve pre-input into the micro-control unit to obtain a hematocrit value, and a concentration value of the analyte is calculated through a correction curve pre-input into the detector by utilizing the HCT value and the DC current value of the analyte which are obtained through calculation.

Example 2

Example 2 has substantially the same characteristics as example 1, except that the electrode layer 2 is a gold electrode layer and the substrate layer 1 is a substrate layer made of polyvinyl chloride.

Example 3

Example 3 has substantially the same characteristics as example 1, except that the substrate layer 1 is a substrate layer made of polypropylene.

Example 3

A detection system with the electrochemical test paper of embodiment 1 or 2, comprising a tester including a micro control unit and a display screen, the tester further having a connector into which the electrochemical test paper is inserted to be connected to the tester; the electrode layer 2 comprises an HCT electrode 21, a counter electrode 22 and a working electrode 23, the micro control unit is used for applying a single-frequency voltage AC signal without direct current bias with a certain amplitude between the HCT electrode 21 and the counter electrode 22, the micro control unit collects original signals and calculates impedance, a corrected HCT value is obtained through calculation of an HCT value pre-input by the micro control unit and an impedance equation, and the micro control unit is connected with the display screen and displays the corrected HCT value on the display screen.

The micro control unit is used for applying a DC signal with constant voltage between the counter electrode and the working electrode 23, collecting an original signal and calculating an analyte concentration value in a blood sample on the electrochemical test paper, and the micro control unit calculates the analyte concentration value through an equation by using a pre-input corrected HCT value and an initial analyte concentration value and displays the concentration value on a display screen.

Claims

1. Electrochemical test paper with hematocrit compensation is characterized in that: comprises a substrate layer (1), an electrode layer (2), an enzyme layer (3), a siphon guide groove (4) and an upper cover plate (5) which are sequentially attached from bottom to top.

2. The electrochemical test strip with hematocrit compensation of claim 1, wherein: the substrate layer (1) is made of any one of polyethylene terephthalate, polyvinyl chloride and polypropylene.

3. The electrochemical test strip with hematocrit compensation of claim 1, wherein: the electrode layer (2) is a gold electrode layer or a silver carbon electrode layer, and the electrode layer (2) comprises an HCT electrode (21), a counter electrode (22) and a working electrode (23).

4. The electrochemical test strip with hematocrit compensation of claim 1, wherein: the upper cover plate (5) is provided with a vent hole.

5. A test system with an electrochemical test strip according to any one of claims 1 to 4, comprising a micro-control unit and a display screen; the method is characterized in that: the electrode layer (2) comprises an HCT electrode (21), a counter electrode (22) and a working electrode (23), the micro control unit is used for applying a single-frequency voltage AC signal without direct current bias between the detection HCT electrode (21) and the counter electrode (22) and calculating a corrected HCT value, and the micro control unit is connected with the display screen and displays the corrected HCT value on the display screen.

6. The detection system of claim 5, wherein: the micro control unit is used for applying a DC signal with constant voltage between the counter electrode and the working electrode (23) and calculating an initial analyte concentration value in a blood sample on the electrochemical test paper, and the micro control unit calculates the analyte concentration value by using a pre-input HCT value and an initial analyte concentration value calibration curve and displays the analyte concentration value on a display screen.