CN215191824U - Colorectal cast-off cell collecting device - Google Patents

Abstract

The utility model provides a colorectal cast-off cell collecting device, which comprises a sampling tube cover, wherein the sampling tube cover defines a first end part, a second end part and a sealed cavity, the first end part and the second end part are respectively arranged at two sides of the sealed cavity, and a sample preserving fluid is arranged in the sealed cavity; the first end part is provided with a first film, the second end part is provided with a second film and a hollow column, and the hollow column is arranged outside the sealed cavity; the first film, the second film and the hollow column are positioned such that: at least a partial region of a region defined by the central through hole of the hollow column has projections on both the first film and the second film in the extending direction of the central through hole.

Description

Colorectal cast-off cell collecting device

Technical Field

The utility model relates to the field of medical equipment, concretely relates to colorectal cast-off cell collection system.

Background

Colorectal cancer (CRC) is a common neoplastic disease of the digestive system and suitable early CRC screening methods are currently lacking. The colorectal endoscope is considered to be an accurate diagnosis mode, but the invasiveness and the requirement of professional operation of the colorectal endoscope cause low patient compliance in clinical practical application.

In recent years, it has become a relatively simple and noninvasive approach to extract exfoliated intestinal epithelial cells or human-derived DNA from feces. However, the presence of large amounts of microorganisms in human feces can interfere with the recovery of cells and DNA, making the collection of exfoliated epithelial cells and human DNA in this manner very limited or difficult.

SUMMERY OF THE UTILITY MODEL

In view of the above technical problems, the present invention provides a device for collecting exfoliated colorectal cells, which includes a sampling tube cap defining a first end, a second end and a sealed cavity, wherein the first end and the second end are respectively located at two sides of the sealed cavity, and a sample preserving fluid is disposed in the sealed cavity; the first end part is provided with a first film, the second end part is provided with a second film and a hollow column, and the hollow column is arranged outside the sealed cavity; the first film, the second film and the hollow column are matched to meet the following requirements: at least a partial region of a region defined by the central through hole of the hollow column has projections on both the first film and the second film in the extending direction of the central through hole.

Further, the collecting device for the colorectal exfoliated cells further comprises a puncturing assembly, wherein the puncturing assembly is matched with the first film, the second film and the hollow column, so that the puncturing assembly is suitable for puncturing the first film and the second film and penetrating into the hollow column.

Further, the tip of the puncture assembly adopts a metal needle tip.

Further, the colorectal exfoliated cell collecting device further comprises a protective sleeve for sleeving the puncture assembly.

Further, a groove is formed in the side wall of the puncture assembly.

Further, a guide structure is disposed within the sealed cavity, the guide structure being adapted to guide the spike assembly to pierce the second membrane along the region defined thereby and into the hollow post.

Further, the guiding structure has a hollow structure and is provided with through holes and/or through slots penetrating the side walls.

Further, the first end portion is provided with an insertion hole through which the first film is exposed.

Further, the puncture assembly comprises an end portion, and the first end portion is further provided with a groove at the insertion hole for matching with the end portion, so that the end portion can be finally arranged in the groove after the puncture assembly is punctured by the insertion hole.

Further, the colorectal exfoliated cell collecting device further comprises a sampling swab, wherein the rod part of the sampling swab is suitable for being inserted into the central through hole of the hollow column, so that the sampling swab is fixedly connected to the sampling tube cover.

Further, the rod part of the sampling swab is of a hollow structure.

Further, the first film is a sealing film provided at the first end portion.

Further, the second film is a sealing film arranged at the second end portion, or the second film and the main body of the sampling tube cover are integrally formed, and the second film is limited by material thickness.

Further, the colorectal exfoliated cell collecting device comprises a sampling tube, and the sampling tube cover and the sampling tube are detachably connected to one end of the second end part.

The colorectal cast-off cell collecting device of the utility model has the advantages that:

1. by pre-installing the sample preservation solution in the tube cap, the sampling head is prevented from contacting the preservation solution before the sample is collected, and the sample can be kept dry.

2. After the sampling, can conveniently pour into the sample through puncture assembly with sample preservation liquid and protect the sample of gathering in the sampling pipe to play the closure effect, prevent that liquid from spilling, guarantee better save the effect.

3. When the puncture assembly is injecting the sample preservation liquid into the sampling pipe, push away the sampling swab to the sampling pipe bottom simultaneously, make the whole submergions of sampling head in the sample preservation liquid to can play better save the effect.

4. The device has simple integral structure and simple and convenient operation when in use.

The conception, the specific structure and the technical effects of the present invention will be further described with reference to the accompanying drawings, so as to fully understand the objects, the features and the effects of the present invention.

Drawings

FIG. 1 is a schematic structural view of a sampling tube and a sampling tube cap of a colorectal exfoliated cell collecting device according to an embodiment of the present invention;

FIG. 2 is an exploded view of the sample tube cover of the embodiment of FIG. 1;

FIG. 3 is a schematic cross-sectional view of the sample tube cover of the embodiment of FIG. 1 in cross-section along its axis;

FIG. 4 is a schematic structural view of a cap body in the sampling tube cap of FIG. 2;

FIG. 5 is a schematic structural view of another perspective of the tube cap body in the sampling tube cap of FIG. 2;

FIG. 6 is a schematic view of the structure of the cover body in the cover of the sampling tube in FIG. 2;

FIG. 7 is a schematic structural view of a puncturing member of the device for collecting exfoliated colorectal cells according to one embodiment of the present invention;

FIG. 8 is a schematic view of the piercing assembly in the piercing member of FIG. 7;

FIG. 9 is a schematic view of the embodiment of FIG. 1 with the sampling tube cap attached to the sampling swab;

fig. 10 is a state reference diagram of the colorectal exfoliated cell collection apparatus of one embodiment of the present invention during use, including the insertion of the spike assembly into the cover of the sampling tube and the ejection of the sampling swab and its dropping into the sampling tube;

fig. 11 is a schematic cross-sectional view of the sampling tube cap of the colorectal exfoliated cell collecting device of an embodiment of the present invention, showing the sampling swab ejected from the hollow column by the piercing assembly, along the axial section of the sampling tube cap, and showing a partially enlarged view of the position of the sampling tube cap.

Description of reference numerals:

100-a sample tube cap, 110-a tube cap body,

111-hollow column, 112-central through hole,

113-a membrane, 114-a guide post,

115-through groove, 120-sealing film,

130-a cover body, 131-an insertion hole,

132-a recess, 140-a sealed cavity,

200-sampling tube, 300-puncturing part,

310-a puncture assembly, 311-an end portion,

312-the shaft, 313-the tip,

314-a recess, 320-a protective sleeve,

400-a sampling swab, 410-a sampling rod,

420 — sampling head.

Detailed Description

In the description of the embodiments of the present invention, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the invention. The drawings are schematic diagrams or conceptual diagrams, and the relationship between the thickness and the width of each part, the proportional relationship between the parts and the like are not completely consistent with actual values.

Fig. 1-9 illustrate a colorectal exfoliated cell collection device including a sampling tube cap 100, a sampling tube 200, a piercing member 300, and a sampling swab 400, according to one embodiment of the present invention. The sampling tube cap 100 is detachably connected to the sampling tube 200, such as by clipping, plugging, screwing, etc.

As shown in fig. 2 to 6, the sampling tube cap 100 includes a cap body 110, a sealing film 120, and a cap body 130. The sealing film 120 is disposed at one end of the cap body 110 and closes the end, so that the cap body 110 and the sealing film 120 define a sealed chamber 140, and the sealing film 120 may be a metal film, a plastic film, or a composite film for sealing a beverage bottle or a medicine. The sealed cavity 140 is filled with a sample preservation solution capable of protecting exfoliated cells or human DNA in the sample from degradation, and in the product processing process, the sample preservation solution is filled into the tube cap main body 110, and then one end of the tube cap main body 110 is sealed through the sealing film 120, so that the sample preservation solution is arranged in the sealed cavity 140.

On the other side of the sealed cavity 140 with respect to the sealing film 120, a hollow column 111 is provided outside the sealed cavity 140, and a sidewall of the sealed cavity 140 at the central through hole 112 of the hollow column 111 is set to a thickness below a preset value to be thinner than that of the sidewall on the same side, thereby being defined as a thin film 113.

In other embodiments, the film 113 is also in the form of a sealing film, in this embodiment, the film 113 is an opening, the opening is communicated with the central through hole 112, a step is arranged at the opening or in the central through hole 112, and the sealing film is arranged at the step, so that the tube cover body 110 and the sealing films at the two ends of the tube cover body jointly define the sealed cavity 140.

As for the arrangement of the shape and position of the sealing film 120, the film 113 and the hollow column 111, it is necessary to satisfy in principle: at least a part of the area defined by the central through hole 112 of the hollow column 111 has a projection on both the sealing film 120 and the film 113 in the extending direction of the central through hole 112, or a certain area defined by the central through hole 112 has a projection on both the sealing film 120 and the film 113 in the extending direction thereof. With respect to the present embodiment, referring to fig. 3, the central through hole defines an area a, a projection of which on the film 113 in the axial direction of the central through hole 112 (i.e., the extending direction thereof) is an area b, and a projection of which on the sealing film 120 is an area c. In the present embodiment, the range of the region b coincides with the range of the film 113, and the range of the region c is smaller than the range of the sealing film 120, so that the entire region (i.e., the region a) defined by the central through hole 112 has projections, i.e., the region b and the region c, on both the film 113 and the sealing film 120 in the axial direction of the central through hole 112.

The above arrangement is intended to allow the linearly extending member to pass through the sealing film 120, the film 113 and the hollow post 111 simultaneously. In this embodiment, a puncturing member 300 is provided, and the puncturing member 300 includes a puncturing assembly 310 and a protective sheath 320, as shown in fig. 7. As shown in FIG. 8, the spike assembly 310 includes a tip 311, a shaft 312, and a tip 313, and can be used to pierce the seal 120 and the membrane 113 and then penetrate the hollow post 111 (i.e., to enter the spike assembly 310 into the central bore 112 of the hollow post 111). In this embodiment, the piercing member 310 is integrally formed, and the sharp end 313 is adapted to cooperate with the sealing membrane 120 and the film 113 to pierce the sealing membrane 120 and the film 113. In other embodiments, the tip 313 is a metal tip that is embedded in and secured to the end of the shaft 312. The protective sheath 320 is a hollow cannula for disposing the shaft 312 and tip 313 of the spike assembly 310 within the cannula thereof, thereby preventing contamination of the shaft 312 and tip 313, and preventing damage to external objects or the contact due to exposure of the tip 313 when not in use.

As shown in fig. 2 and 3, the cover 130 covers the sealing film 120 and is fixedly connected to the tube cap body 110, for example, by clipping, inserting, screwing, or adhering. As shown in fig. 6, the cover 130 is provided with an insertion hole 131, and the sealing film 120 is exposed only in the area defined by the insertion hole 131, so that the insertion hole 131 defines the position where the puncture assembly 310 pierces the sealing film 120, facilitating its alignment with the film 113 and the central through hole 112 after piercing the sealing film 120. The cover 130 is further provided with a groove 132, the through hole 131 is opened at the bottom of the groove 132, the shape and contour of the groove 132 are matched with the end 311 of the puncturing assembly 310, when the puncturing assembly 310 is punctured by the insertion hole 131, the end 311 can be finally arranged in the groove 132, the bottom of the groove 132 defines the final depth that the puncturing assembly 310 can enter, in the embodiment, the height of the end 311 is substantially the same as the depth of the groove 132, so that when the bottom of the end 311 contacts the bottom of the groove 132, the top of the end 311 is substantially flush with the upper surface of the cover 130. In other embodiments, only the insertion hole is provided in the cover 130, where the upper surface of the cover 130 defines the final depth that the spike assembly 310 can enter.

As shown in fig. 3 and 4, a guide post 114 is further disposed in the sealing cavity 140, and in this embodiment, the guide post 114 is a hollow cylinder structure and is disposed coaxially with the central through hole 112. The side wall of the hollow cylinder of the guide column 114 is provided with a through groove 115 penetrating through the side wall, so that no matter where the liquid level of the sample preservation liquid in the sealed cavity is, the sample preservation liquid can flow into the hollow area of the guide column 114. In other embodiments, the through slots 115 are replaced with through holes through the guide post sidewalls so that the above-described effects can also be achieved. The penetration member 310 penetrates the sealing film 120 through the insertion hole 131, and then penetrates the hollow region of the guide post 114 to guide it to align with the film 113 and the central through hole 112, thereby further improving the operation accuracy.

As shown in fig. 9, the sampling swab 400 includes a sampling rod 410 and a sampling head 420, and the central through-hole 112 is sized to match the outer diameter of the sampling rod 410 such that an end of the sampling rod 410 can be inserted into the central through-hole 112 and the sampling swab 400 can be fixedly attached to the cap body 110. The sampling head 420 may be a flexible material such as nylon flocking, cotton, etc. to facilitate taking a sample; the sampling head 420 may also take the form of a sampling spoon, sampling wand, or the like, to allow for the collection of samples both of the anus/perianal area and also for the direct collection of fecal samples after defecation.

When the colorectal exfoliated cell collecting apparatus of this embodiment performs sampling, a user holds the sampling tube 200, unscrews the sampling tube cap 100, holds the sampling tube cap 100, and scrapes the large intestine mucosal cell layer from the anus/perianal region by the sampling swab 400 fixed to the sampling tube cap 100, specifically, the sampling head 420 at one end of the sampling swab 400. After the sampling is completed, the sampling swab 400 is placed back into the sampling tube 200 and the sampling tube cap 100 is tightened. The puncturing member 300 is taken out, the protecting sleeve 320 on the puncturing assembly 310 is removed, the tip 313 of the puncturing assembly 310 is aligned downwards to the insertion hole 131, the end 311 of the puncturing assembly 310 is pushed slowly, the tip 313 penetrates the sealing film 120 and the thin film 113 in sequence and enters the central through hole 112 of the hollow column 111, and after the sampling rod 410 arranged in the central through hole 112 is abutted, the sampling rod 410 is further pushed away from the hollow column 111, so that the sampling swab 400 falls into the bottom of the sampling tube 200 integrally, and refer to fig. 10 and 11. In the process, after the tip 313 pierces the film 113, the sample preservation solution in the sealed cavity 140 flows out from the broken position of the film 113 into the central through hole 112, and as the sampling swab 400 is pushed out of the central through hole 112 and falls into the sampling tube 200, the sample preservation solution also falls into the sampling tube 200 along the central through hole 112, and the liquid level of the sample preservation solution in the sampling tube 200 can submerge the sampling head 420 of the sampling swab 400 falling into the bottom of the sampling tube 200, so as to protect the cells or the human source DNA in the sample from being degraded. Pushing on the end 311 is continued until the end 311 abuts against the bottom of the groove 132, thereby effectively closing the insertion hole 131, which is more advantageous for sample preservation, and thus sample collection.

After the tip 313 of the puncturing member 310 punctures the film 113, in order to make the sample preservation solution flow out of the sealed cavity 140 more smoothly, in the embodiment, four grooves 314 are provided on the shaft 312 of the puncturing member 310, and the grooves 314 extend to the tip 313 along the length direction of the shaft 312, as shown in fig. 8.

In other embodiments, the sampling shaft 410 of the sampling swab 400 is a hollow shaft, in which the piercing member 310, in the extreme position after being inserted into the sampling tube cap 100, does not touch the sampling shaft 410 in the central through hole 112 after the tip 313 pierces the film 113, and the sample preservation fluid flowing from the sealed cavity 140 into the central through hole 112 flows along the hollow area of the sampling shaft 410 to the sampling head 420, permeates the sampling head 420, flows into the bottom of the sampling tube 200, and submerges the sampling head 420. To this end, the length of the sampling swab 400 and/or the length of the sampling tube 200 need to be adapted to enable the sampling head 420 to be adjacent to the bottom of the sampling tube 200 so that the exiting sample preservation fluid is sufficient to submerge it. Furthermore, the secure attachment of the sampling rod 410 to the sampling tube cap 100 can be in a more secure manner, such as by adhesive bonding, since the two do not need to be separated during use.

The foregoing has described in detail preferred embodiments of the present invention. It should be understood that numerous modifications and variations can be devised by those skilled in the art in light of the teachings of the present invention without undue experimentation. Therefore, the technical solutions that can be obtained by a person skilled in the art through logic analysis, reasoning or limited experiments based on the prior art according to the concepts of the present invention should be within the scope of protection defined by the claims.

Claims (14)

1. A colorectal exfoliated cell collection device comprises a sampling tube cover, wherein the sampling tube cover defines a first end part, a second end part and a sealed cavity, the first end part and the second end part are respectively positioned at two sides of the sealed cavity, and a sample preserving fluid is arranged in the sealed cavity; the first end part is provided with a first film, the second end part is provided with a second film and a hollow column, and the hollow column is arranged outside the sealed cavity; the first film, the second film and the hollow column are matched to meet the following requirements: at least a partial region of a region defined by the central through hole of the hollow column has projections on both the first film and the second film in the extending direction of the central through hole.

2. The colorectal exfoliated cell harvesting apparatus of claim 1, further including a piercing assembly cooperating with the first membrane, the second membrane and the hollow column so as to be adapted to pierce the first membrane and the second membrane and into the hollow column using the piercing assembly.

3. The colorectal exfoliated cell harvesting apparatus of claim 2, wherein the tip of the piercing assembly is a metal needle tip.

4. The colorectal exfoliated cell harvesting apparatus of claim 2, further including a protective sheath for sheathing the spike assembly.

5. The colorectal exfoliated cell harvesting apparatus of claim 2, wherein the piercing assembly has a recess in a side wall thereof.

6. The colorectal exfoliated cell harvesting device of claim 2, wherein a guide structure is disposed within the sealed cavity, the guide structure being adapted to guide the piercing assembly to pierce the second membrane along the region defined thereby and penetrate the hollow post.

7. A colorectal exfoliated cell harvesting device according to claim 6, wherein the guide structure has a hollow structure and is provided with through holes and/or through slots passing through the side wall.

8. The colorectal exfoliated cell harvesting device of claim 2, wherein the first end portion is provided with an insertion hole through which the first membrane is exposed.

9. The colorectal exfoliated cell harvesting apparatus of claim 8, wherein the piercing assembly includes an end portion, the first end portion further provided with a recess at the insertion hole for mating with the end portion, such that the end portion may be finally disposed within the recess after the piercing assembly is pierced by the insertion hole.

10. The colorectal exfoliated cell collecting device of claim 1, further comprising a sampling swab, the stem portion of which is adapted to be inserted into the central through hole of the hollow column, so that the sampling swab is fixedly attached to the sampling tube cover.

11. The colorectal exfoliated cell collection apparatus of claim 10, wherein the shaft of the sampling swab is hollow.

12. The colorectal exfoliated cell collection device of claim 1, wherein the first membrane is a sealing membrane disposed at the first end portion.

13. The colorectal exfoliated cell harvesting device of claim 1, wherein the second membrane is a sealing membrane disposed at the second end, or the second membrane is integrally formed with the body of the sampling tube cap, the second membrane being defined by a material thickness.

14. The colorectal exfoliated cell collection apparatus of claim 1, further including a sampling tube, the sampling tube cap and the sampling tube being removably attached to the end at which the second end is located.

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