CN215180245U - Cell antigen quantitative determination kit - Google Patents

Cell antigen quantitative determination kit Download PDF

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Publication number
CN215180245U
CN215180245U CN202121477550.4U CN202121477550U CN215180245U CN 215180245 U CN215180245 U CN 215180245U CN 202121477550 U CN202121477550 U CN 202121477550U CN 215180245 U CN215180245 U CN 215180245U
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CN
China
Prior art keywords
mounting groove
kit
reagent bottle
kit body
cell antigen
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Expired - Fee Related
Application number
CN202121477550.4U
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Chinese (zh)
Inventor
张燕
陈旭昕
孟激光
韩志海
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6th Medical Center of PLA General Hospital
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6th Medical Center of PLA General Hospital
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Priority to CN202121477550.4U priority Critical patent/CN215180245U/en
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Publication of CN215180245U publication Critical patent/CN215180245U/en
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Abstract

The utility model belongs to the technical field of cell antigen detection equipment, and discloses a cell antigen quantitative detection kit, which comprises a kit body, wherein one side of the top of the kit body is hinged with a cover plate, and the cover plate is connected with the kit body through a buckle to realize closing; a buffer plate is fixedly arranged in the inner cavity of the kit body, and mounting grooves for accommodating a buffer solution reagent bottle, an EP tube, an antigen detection reagent bottle, a magnetic bead standard reagent bottle and a flow tube are formed in the buffer plate; the top of buffer board is fixed with the limiting plate, is equipped with the spacing hole corresponding with the mounting groove on the limiting plate, and this internal buffer board that lies in of kit and limiting plate have the cavity between, is equipped with temperature sensor in the cavity, and the last face of limiting plate is equipped with temperature monitor, controller and siren, and temperature sensor, temperature monitor and siren are connected with the controller electricity respectively. The utility model discloses convenient to carry can regulate and control this internal reasonable temperature that is in of kit, conveniently carries out quantitative determination to cell antigen.

Description

Cell antigen quantitative determination kit
Technical Field
The utility model belongs to the technical field of cell antigen check out test set, concretely relates to cell antigen quantitative determination kit.
Background
Malignant epithelial cell tumor belongs to one kind of cancer, is the most serious disease endangering human health at present, the tumor tissue is composed of parenchyma and stroma, the tumor parenchyma is tumor cells, is the main component of tumor, and has tissue source specificity. The tumor cells often generate new or over-expressed cell antigen substances in the processes of tumor occurrence and development, and the detection of the expression level of tumor specific antigens and tumor related antigens has important significance in the aspects of scientific research, auxiliary diagnosis, prognosis judgment and the like, and especially can play a great role in tumor immunotherapy, such as ADC drugs, CAR-T and the like.
If the antigen expression level of tumor cells needs to be quantitatively analyzed and detected in the prior art, the operation is complex and inconvenient, the detection speed is slow, the efficiency is low, multiple medicaments need to be used, multiple groups of detection results are obtained, multiple reagent containers need to be used for subpackaging, and the carrying and the storage are inconvenient.
SUMMERY OF THE UTILITY MODEL
In order to solve the problem of inconvenient operation of tumor cell antigen detection in the prior art, the utility model provides a cell antigen quantitative determination kit.
The utility model discloses the technical scheme who adopts does: a cell antigen quantitative detection kit comprises a kit body, wherein a cover plate is hinged to one side of the top of the kit body, and the cover plate is connected with the kit body through a buckle to realize closing; a buffer plate is fixedly arranged in the inner cavity of the kit body, and a first mounting groove for accommodating a buffer solution reagent bottle, a second mounting groove for accommodating an EP (EP) tube, a third mounting groove for accommodating an antigen detection reagent bottle, a fourth mounting groove for mounting a magnetic bead standard reagent bottle and a fifth mounting groove for mounting a flow tube are arranged on the buffer plate; a limiting plate is fixed above the buffer plate, and limiting holes matched with the buffer solution reagent bottle, the EP tube, the antigen detection reagent bottle, the magnetic bead standard reagent bottle and the flow tube are formed in the limiting plate; a cavity is arranged between the buffer plate and the limiting plate in the kit body; be equipped with temperature sensor in the cavity, the last face of limiting plate is equipped with temperature monitor, controller and siren, temperature sensor, temperature monitor and siren are connected with the controller electricity respectively.
Optionally, a cold insulation bag placing cavity and a sealing cover used for sealing the cold insulation bag placing cavity are formed in one side of the kit body, and a groove is further formed in the buffer plate and enables the cold insulation bag placing cavity to be communicated with the cavity through the groove.
Optionally, the cold-keeping bag placing cavities are provided with two groups and distributed on the left side and the right side of the bottom of the inner cavity of the kit body.
Optionally, one end of the sealing cover is hinged to the side wall of the kit body, and the other end of the sealing cover is connected through a buckle to seal the cold-insulation bag placing cavity.
Optionally, the top of the kit body is further provided with a step wall, the first mounting groove, the second mounting groove, the third mounting groove, the fourth mounting groove and the fifth mounting groove are all arranged below the step wall, and when the cover plate is closed, the upper part of the step wall is attached to the cover plate; the height of the step wall is matched with the thicknesses of a buffer solution reagent bottle, an EP (EP) tube, an antigen detection reagent bottle, a magnetic bead standard reagent bottle and a bottle cap of a flow tube.
Optionally, a sealing groove is formed in the circumferential direction of the top step of the step wall, and a sealing ring matched with the sealing groove is arranged on the cover plate.
As optional, the first mounting groove, the second mounting groove, the third mounting groove, the fourth mounting groove, and the fifth mounting groove are disposed in a staggered manner with the limiting hole located above the first mounting groove, the second mounting groove, the third mounting groove, the fourth mounting groove, and the fifth mounting groove, so that the buffer solution reagent bottle, the EP tube, the antigen detection reagent bottle, the magnetic bead standard reagent bottle, and the flow tube are respectively installed in the first mounting groove, the second mounting groove, the third mounting groove, the fourth mounting groove, and the fifth mounting groove in an inclined manner.
Optionally, a clamping end is arranged on one side of the cover plate, a first handle is arranged on the side face of the kit body, and a buckling end matched with the clamping end is arranged at the top of the first handle.
Optionally, a second handle is further arranged on the reagent box body and located on the opposite side of the first handle.
The utility model has the advantages that:
the utility model discloses with buffer solution reagent bottle, EP pipe, antigen detection reagent bottle, magnetic bead standard article reagent bottle and STREAMING pipe unified deposit in the kit body, the buffer board can provide absorbing effect for it when placing above-mentioned reagent bottle and test tube, and spacing hole on the limiting plate then forms the restraint to reagent bottle and test tube, avoids its emergence to rock, makes things convenient for taking of its inside reagent. And a cavity is formed between the buffer plate and the limiting plate in the reagent box, so that the storage temperature of the reagent bottle can be well controlled, and the reagent can be stored conveniently. The utility model has the advantages of convenient to carry, easy operation, and then make things convenient for the detection of tumour cell antigen.
The advantages of the invention are not limited to the description, but rather are described in greater detail in the detailed description for better understanding.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
Fig. 1 is a schematic structural diagram of the present invention;
FIG. 2 is a schematic structural view of a middle buffer plate according to the present invention;
in the figure: 1-a kit body, 2-a cover plate, 3-a limiting plate, 31-a first limiting hole, 32-a second limiting hole, 33-a third limiting hole, 34-a fourth limiting hole, 35-a fifth limiting hole, 36-a sixth limiting hole, 4-a cold-insulation bag placing cavity, 5-a sealing cover, 6-a buffer plate, 61-a first mounting groove, 62-a second mounting groove, 63-a third mounting groove, 64-a fourth mounting groove, 65-a fifth mounting groove, 66-a sixth mounting groove, 67-a slot, 11-a step wall, 12-a sealing groove, 13-a buckling end, 21-a clamping end and 22-a sealing ring.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention, as generally described and illustrated in the figures herein, may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
It should be noted that, in the present invention, the embodiments and features of the embodiments may be combined with each other without conflict.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.
In the description of the embodiments of the present invention, it should be noted that the indication of the position or the positional relationship is based on the position or the positional relationship shown in the drawings, or the position or the positional relationship which is usually placed when the product of the present invention is used, or the position or the positional relationship which is conventionally understood by those skilled in the art, and is only for the convenience of describing the present invention and simplifying the description, but it is not to indicate or suggest that the indicated device or element must have a specific position, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used merely to distinguish one description from another, and are not to be construed as indicating or implying relative importance.
In the description of the embodiments of the present invention, it should be further noted that unless otherwise explicitly stated or limited, the terms "disposed" and "connected" should be interpreted broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; may be directly connected or indirectly connected through an intermediate. For those skilled in the art, the specific meanings of the above terms in the present invention can be understood according to the specific situations and the drawings of the specification, and the technical solutions in the embodiments of the present invention are clearly and completely described. The components of embodiments of the present invention, as generally described and illustrated in the figures herein, may be arranged and designed in a wide variety of different configurations.
The invention will be further explained with reference to the drawings and the specific embodiments.
As shown in fig. 1-2, the utility model provides a cell antigen quantitative detection kit, which comprises a cuboid kit body 1, wherein a cover plate 2 is hinged on one side of the top of the kit body 1, and the cover plate 2 is connected with the kit body 1 through a buckle to realize closing; the middle part of the inner cavity of the kit body 1 is provided with a buffer plate 6, the periphery of the buffer plate 6 is fixed on the side wall of the inner cavity of the kit body 1, the buffer plate 6 is provided with a first mounting groove 61 for accommodating a buffer solution reagent bottle, a second mounting groove 62 for accommodating an EP (EP) tube, a third mounting groove 63 for accommodating an antigen detection reagent bottle, a fourth mounting groove 64 for accommodating a magnetic bead standard reagent bottle and a fifth mounting groove 65 for accommodating a flow tube; a limiting plate 3 is further fixed above the buffer plate 6, and a first limiting hole 31, a second limiting hole 32, a third limiting hole 33, a fourth limiting hole 34 and a fifth limiting hole 35 which are matched with a buffer solution reagent bottle, an EP tube, an antigen detection reagent bottle, a magnetic bead standard sample reagent bottle and a flow tube are arranged on the limiting plate 3; the reagent bottle and the test tube are directly inserted into the corresponding limiting holes in the installation process, and the bottoms of the reagent bottle and the test tube are installed in the corresponding installation grooves; a cavity is arranged between the buffer plate 6 and the limiting plate 3 in the kit body 1. The sizes of the first installation groove 61, the second installation groove 62, the third installation groove 63, the fourth installation groove 64 and the fifth installation groove 65 are correspondingly configured according to the dosage of the reagents required in the buffer solution reagent bottle, the EP tube, the antigen detection reagent bottle, the magnetic bead standard reagent bottle and the flow tube, and the size of the limiting hole is correspondingly adjusted. Be equipped with temperature sensor in the cavity, the last face of limiting plate is equipped with temperature monitor, controller and siren, temperature sensor, temperature monitor and siren are connected with the controller electricity respectively. The temperature of the reagent in the reagent bottle and the reagent in the test tube needs to be controlled to be 2-8 ℃, so that the alarm is triggered to give an alarm to remind a user of controlling the temperature of the cavity in the reagent kit body 1 when the temperature monitored by the temperature sensor exceeds the range of 2-8 ℃ by the controller; and the display can show the temperature of the cavity of the kit body 1 in real time, so that the regulation and control of the temperature of the cavity of the kit body 1 are conveniently controlled, and the current use temperature can be displayed.
In addition, in order to avoid the situation that a part of the reagents are required to be used more greatly and no corresponding storage position exists on the reagent box body 1, in another embodiment, the buffer plate 6 and the limiting plate 3 are respectively provided with a corresponding sixth mounting groove 66 and a corresponding sixth limiting hole 36 for placing a spare reagent bottle or test tube; according to actual needs, two groups of first mounting grooves 61 are arranged, and four groups of second mounting grooves 62, third mounting grooves 63, fourth mounting grooves 64, fifth mounting grooves 65 and sixth mounting grooves 66 are arranged side by side; the first mounting groove 61, the second mounting groove 62 and the third mounting groove 63 are sequentially arranged on one side of the buffer plate 6 in parallel, and the fourth mounting groove 64, the fifth mounting groove 65 and the sixth mounting groove 66 are sequentially arranged on the other side of the buffer plate 6 in parallel; first mounting groove and fourth mounting groove 64, second mounting groove 62 and fifth mounting groove 65 and third mounting groove 63 and fourth mounting groove 64 all locate same water flat line on, like this, the rational arrangement of reagent bottle and test tube makes overall structure compacter, also very does benefit to the detection operation of follow-up tumour cell antigen simultaneously.
In the use of this kit, the buffer solution reagent bottle that will prepare to accomplish, the EP pipe, antigen detection reagent bottle, this kit body 1 corresponds is put into respectively to magnetic bead standard substance reagent bottle and STREAMING pipe first mounting groove 61, second mounting groove 62, third mounting groove 63, fourth mounting groove 64, fifth mounting groove 65 and sixth mounting groove 66, when carrying out tumour cell antigen detection, directly take this kit go on take of corresponding reagent can, to tumour cell antigen quantitative determination, the operation of whole process can be simplified greatly in the use of this kit.
In order to reduce the temperature of the cavity in the reagent kit body 1, in another embodiment, a cold-keeping bag placing cavity 4 and a sealing cover 5 for sealing the cold-keeping bag placing cavity 4 are opened on one side of the reagent kit body 1, and a slot 67 is further formed on the buffer plate 6 and the cold-keeping bag placing cavity 4 is communicated with the cavity through the slot 67. The cold insulation bag or other independent temperature control objects are placed in the cold insulation bag placing cavity 4, cold air enters the cavity through the open groove 67, and the temperature of the cavity of the kit body 1 can be controlled within the range of 2-8 ℃ according to the display of the temperature display.
In order to adjust the temperature of the cavity of the reagent kit body 1 more quickly, in another embodiment, two groups of the cold-keeping bag placing cavities 4 are arranged and distributed at the left and right sides of the bottom of the inner cavity of the reagent kit body 1.
In order to further guarantee the temperature of the cavity of the kit body 1, a packaging box is arranged outside the kit body 1 and used for better sealing the kit body 1.
In order to facilitate the opening and closing of the sealing cover 5, in another embodiment, one end of the sealing cover 5 is hinged on the side wall of the reagent box body 1, and the other end of the sealing cover 5 is connected by a snap to close the cold-keeping bag placing cavity 4.
In order to facilitate the storage of reagent bottles, in another embodiment, the top of the reagent kit body 1 is further provided with a step wall 11, the first installation groove 61, the second installation groove 62, the third installation groove 63, the fourth installation groove 64 and the fifth installation groove 65 are all arranged below the step wall 11, and when the cover plate 2 is closed, the upper part of the step wall 11 is attached to the cover plate 2; the height of the step wall 11 is matched with the thicknesses of a buffer solution reagent bottle, an EP tube, an antigen detection reagent bottle, a magnetic bead standard reagent bottle and a bottle cap of a flow tube. Therefore, when the buffer solution reagent bottle, the EP tube, the antigen detection reagent bottle, the magnetic bead standard reagent bottle and the flow tube are stored, the bottle covers are closed, and the reagent in the bottle is prevented from being polluted.
In order to prevent the reagent bottles from being damaged by vibration or impact during storage and transportation, in another embodiment, the inner side walls of the first, second, third, fourth, fifth and sixth mounting grooves 61, 62, 63, 64, 65 and 66 are further provided with buffer side walls, and preferably, the buffer plate 6 is made of foam.
In order to achieve better sealing performance of the reagent kit body 1, in another embodiment, a sealing groove 12 is formed on the top step of the step wall 11 in the circumferential direction, and a sealing ring 22 adapted to the sealing groove 12 is formed on the cover plate 2.
In order to increase the storage stability of the reagent bottles, in another embodiment, the first mounting groove 61, the second mounting groove 62, the third mounting groove 63, the fourth mounting groove 64, and the fifth mounting groove 65 are disposed in a staggered manner with respect to the limiting hole located above the first mounting groove, so that the buffer reagent bottle, the EP tube, the antigen detection reagent bottle, the magnetic bead standard reagent bottle, and the flow tube are obliquely mounted in the first mounting groove 61, the second mounting groove 62, the third mounting groove 63, the fourth mounting groove 64, and the fifth mounting groove 65, respectively; simultaneously, the incline direction of above-mentioned reagent bottle and test tube is unanimous, makes reagent bottle or test tube slope put corresponding the mounting groove like this, and the stability of reagent bottle and test tube can obtain fine reinforcing.
In order to facilitate taking the reagent kit body 1, in another embodiment, a clamping end 21 is disposed on one side of the cover plate 2, a first handle is disposed on a side surface of the reagent kit body 1, and a fastening end 12 matched with the clamping end 21 is disposed at the top of the first handle.
Since the liquid in the reagent bottle can cause pollution due to the inclined long-term basic bottle cap, in another embodiment, a second handle is further arranged on the reagent box body 1 at the opposite side of the first handle.
In order to make the reagent bottle be well identified, corresponding labels are pasted outside the buffer solution reagent bottle, the EP tube, the antigen detection reagent bottle, the magnetic bead standard reagent bottle and the flow tube.
The kit specifically comprises the following steps in the using process of quantitative detection of cell antigens:
(1) washing the tumor cells to be detected and the magnetic bead standard substance by using 1 mLBuffer;
(2) centrifuging at 1000rpm for 3min, and adding 100 μ L Buffer for resuspension;
(3) adding required antigen detection reagents into different samples to be detected and corresponding standard products thereof according to test requirements;
(4) incubating at 4 deg.C for 30 min;
(5) adding 500 mu LBuffer, centrifuging at 1000rpm for 3 min;
(6)300 u LBuffer heavy suspension;
(7) and (5) performing flow detection, and calculating to obtain a quantitative result.
The magnetic bead standard substance is stored in the magnetic bead standard substance reagent bottle, the Buffer is stored in the Buffer solution reagent bottle, the antigen detection reagent can be CD20/BCMA/CD123/Mesothelin/HER2/CD33 and the corresponding standard substance thereof, in the embodiment, CD33/BCMA/CD123/HER2 is selected and stored in the antigen detection reagent bottle.
The utility model discloses can carry out quantitative determination to cell antigen, have and carry, store safe convenient, easy and simple to handle, can use a sample multinomial detection, advantage such as result demonstration is rapid.
The present invention is not limited to the above-mentioned optional embodiments, and any other products in various forms can be obtained by anyone under the teaching of the present invention, and any changes in the shape or structure thereof, all the technical solutions falling within the scope of the present invention, are within the protection scope of the present invention.

Claims (9)

1. A cell antigen quantitative detection kit is characterized in that: the kit comprises a kit body, wherein a cover plate is hinged to one side of the top of the kit body, and the cover plate is connected with the kit body through a buckle to realize closing; a buffer plate is fixedly arranged in the inner cavity of the kit body, and a first mounting groove for accommodating a buffer solution reagent bottle, a second mounting groove for accommodating an EP (EP) tube, a third mounting groove for accommodating an antigen detection reagent bottle, a fourth mounting groove for accommodating a magnetic bead standard reagent bottle and a fifth mounting groove for accommodating a flow tube are formed in the buffer plate; a limiting plate is fixed above the buffer plate, limiting holes matched with the buffer solution reagent bottle, the EP tube, the antigen detection reagent bottle, the magnetic bead standard reagent bottle and the flow tube are formed in the limiting plate, and a cavity is formed between the buffer plate and the limiting plate in the kit body; be equipped with temperature sensor in the cavity, the last face of limiting plate is equipped with temperature monitor, controller and siren, temperature sensor, temperature monitor and siren are connected with the controller electricity respectively.
2. The kit for quantitatively detecting a cell antigen according to claim 1, wherein: the kit body one side is seted up the cold insulation bag and is placed the chamber and be used for sealed this cold insulation bag and place the sealed lid in chamber, still be equipped with the fluting on the buffer board and make the cold insulation bag place chamber and cavity intercommunication through the fluting.
3. The kit for quantitatively detecting a cell antigen according to claim 2, wherein: the cold insulation bag placing cavities are provided with two groups and distributed on the left side and the right side of the bottom of the inner cavity of the kit body.
4. The kit for quantitatively detecting a cell antigen according to claim 2, wherein: one end of the sealing cover is hinged to the side wall of the kit body, and the other end of the sealing cover is connected through a buckle to seal the cold-insulation bag placing cavity.
5. The kit for quantitatively detecting a cell antigen according to claim 1, wherein: the kit body is characterized in that the top of the kit body is also provided with a step wall, the first mounting groove, the second mounting groove, the third mounting groove, the fourth mounting groove and the fifth mounting groove are all arranged below the step wall, and when the cover plate is closed, the upper part of the step wall is attached to the cover plate; the height of the step wall is matched with the thicknesses of a buffer solution reagent bottle, an EP (EP) tube, an antigen detection reagent bottle, a magnetic bead standard reagent bottle and a bottle cap of a flow tube.
6. The kit for quantitatively detecting a cell antigen according to claim 5, wherein: the top step of step wall is equipped with the seal groove on its circumference, be equipped with on the apron with seal ring of seal groove looks adaptation.
7. The kit for quantitatively detecting a cell antigen according to claim 1, wherein: first mounting groove, second mounting groove, third mounting groove, fourth mounting groove, fifth mounting groove and the spacing hole dislocation set who is located its top for buffer solution reagent bottle, EP pipe, antigen detect reagent bottle, magnetic bead standard reagent bottle and STREAMING pipe slope respectively and install in first mounting groove, second mounting groove, third mounting groove, fourth mounting groove and fifth mounting groove.
8. The kit for quantitatively detecting a cell antigen according to claim 1, wherein: a clamping end is arranged on one side of the cover plate, a first handle is arranged on the side face of the kit body, and a buckling end matched with the clamping end is arranged at the top of the first handle.
9. The kit for quantitatively detecting a cell antigen according to claim 8, wherein: a second handle is further arranged on the kit body and is positioned on the opposite side of the first handle.
CN202121477550.4U 2021-06-30 2021-06-30 Cell antigen quantitative determination kit Expired - Fee Related CN215180245U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202121477550.4U CN215180245U (en) 2021-06-30 2021-06-30 Cell antigen quantitative determination kit

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202121477550.4U CN215180245U (en) 2021-06-30 2021-06-30 Cell antigen quantitative determination kit

Publications (1)

Publication Number Publication Date
CN215180245U true CN215180245U (en) 2021-12-14

Family

ID=79381545

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202121477550.4U Expired - Fee Related CN215180245U (en) 2021-06-30 2021-06-30 Cell antigen quantitative determination kit

Country Status (1)

Country Link
CN (1) CN215180245U (en)

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CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20211214

CF01 Termination of patent right due to non-payment of annual fee