CN215162535U - Cell sorting device and cell sorting and concentrating system - Google Patents

Abstract

The utility model relates to a biomedical instrument technical field especially relates to concentrated system is selected separately to cell sorting unit and cell, cell sorting unit includes the stock solution bucket, the straw subassembly and presses the subassembly, the straw subassembly includes straw and cock body, the stock solution bucket is equipped with first through-hole, the cock body sets up in first through-hole department, the second through-hole of axial extension of stock solution bucket is followed to the cock body structure, the straw passes in the second through-hole gets into the stock solution bucket, the cock body is including flexible section, the one end and the stock solution bucket of flexible section are connected, the other end and straw fixed connection, it is flexible to press the axial direction that the subassembly can drive flexible section along the stock solution bucket, remove with the drive straw. Through the cooperation of pressing subassembly and straw subassembly, can realize totally closed full automatic cell and select separately or concentrate, replace prior art's manual selection or concentrate, improve and select separately or concentrated efficiency and precision, avoid selecting separately or the external pollution to cell solution of concentration in-process simultaneously.

Description

Cell sorting device and cell sorting and concentrating system

Technical Field

The utility model relates to a biomedical instrument technical field especially relates to cell sorting unit and cell sorting concentration system.

Background

In the field of cell medicine, it is often necessary to inoculate cells collected from a living body to a patient, either directly or after in vitro culture. In order to use cells collected or cultured from a living body for therapy, it is necessary to separate the cells from blood, that is, to sort PBMCs (peripheral blood mononuclear cells) as cells, and to remove impurities and a culture medium and concentrate them to a cell concentration suitable for therapy. For the purpose of sorting and concentrating the cellular PBMCs, it is generally performed using centrifugation.

When the conventional equipment in the prior art is used for sorting and concentrating the PBMC, the PBMC is generally sorted and concentrated during centrifugation, so that the damage to cells is easily caused, the achievable concentration volume is also small, generally about 300-.

SUMMERY OF THE UTILITY MODEL

The utility model provides a concentrated system is selected separately to cell sorting unit and cell for manual operation sorting process causes the pollution very easily among the solution prior art, and the defect that sorting efficiency is low moreover realizes through the cooperation of pressing down subassembly and straw subassembly, has replaced prior art's manual sorting, improves sorting efficiency and precision, avoids the effect of the external pollution to the cell solution of sorting process simultaneously.

The utility model provides a cell sorting unit, including stock solution bucket, straw subassembly and pressing assembly, the straw subassembly includes straw and cock body, the stock solution bucket is equipped with first through-hole, the cock body set up in first through-hole department, the cock body structure is followed the axially extended second through-hole of stock solution bucket, the straw passes the second through-hole and gets into in the stock solution bucket, the cock body is including flexible section, the one end of flexible section with the stock solution bucket is connected, the other end with straw fixed connection, pressing assembly can drive flexible section is followed the axial direction of stock solution bucket is flexible, with the drive the straw removes.

According to the utility model provides a pair of cell sorting unit, the cock body still includes the canned paragraph, the canned paragraph with the coaxial setting of flexible section.

According to the utility model provides a pair of cell sorting unit, flexible section includes extensible member and first end plate, the one end of extensible member with stock solution bucket butt, the other end with first end plate is connected, the straw passes first end plate and with first end plate fixed connection.

According to the utility model provides a pair of cell sorting unit, the extensible member cover is located the outside of canned paragraph, the canned paragraph with first end plate is connected.

According to the utility model provides a pair of cell sorting unit, the canned paragraph with be provided with the sealing ring between the first through-hole.

According to the utility model provides a pair of cell sorting unit, flexible section includes annular second end plate, the inboard of second end plate with the canned paragraph is connected, a terminal surface of second end plate with the extensible member is connected, another terminal surface with the stock solution bucket butt.

According to the utility model provides a pair of cell sorting unit, the straw subassembly still including be used for with press the third end plate of subassembly contact, the third end plate set up in on the cock body, the cock body with have between the third end plate and be used for supplying the clearance that the straw passed.

According to the utility model provides a pair of cell sorting unit still includes supporting seat and driving piece, the supporting seat level sets up, the stock solution bucket with the supporting seat rotates through the pivot to be connected, the pivot level set up and with the axial verticality of stock solution bucket, the driving piece with supporting connection, with the drive the supporting seat around with the axial vertically rotation axial rotation of pivot.

According to the utility model provides a pair of cell sorting unit, the supporting seat is equipped with leads to the liquid pipe, the straw with lead to the liquid pipe intercommunication.

According to the utility model provides a pair of cell sorting unit, lead to the liquid pipe with the coaxial setting of supporting seat, the adapter sleeve and pipeline intercommunication are passed through to the upper end that leads to the liquid pipe, be equipped with in the adapter sleeve be used for with pipe connection's bearing, the pipeline with set up the sealing washer between the inner wall of adapter sleeve.

According to the utility model provides a pair of cell sorting unit, the stock solution bucket is keeping away from the upper portion of a rotatory axial lateral barrel wall of supporting seat is equipped with the third through-hole, the third through-hole with the inside intercommunication of stock solution bucket, third through-hole department is equipped with the ventilated membrane.

According to the utility model provides a pair of cell sorting unit, the supporting seat structure from the edge to the sunken recess of rotatory axial of supporting seat, the stock solution bucket set up in the recess.

The utility model discloses still provide concentrated system is selected separately to cell, including controlling means, supply the liquid device and as above cell sorting unit, supply the liquid device with the driving piece all with controlling means connects, supply the liquid device to include support and liquid way passageway, the support is used for placing the liquid bag, liquid way passageway with stock solution bucket intercommunication.

The utility model provides a cell sorting unit, the vertical placing of stock solution bucket, its inside cell solution that treats sorting of centrifugation layering that is equipped with, first through-hole is seted up at the top of stock solution bucket, the cock body is installed to first through-hole department, the inside intercommunication of cock body structure second through-hole and stock solution bucket, the cock body is located the outside part of stock solution bucket and is flexible section, flexible section is compressible when receiving pressure, the time back extension reconversion that disappears of pressure, the straw is earlier inserted by the outside and is passed the second through-hole portion in the flexible section, pass the second through-hole portion that is located the inside stopper body of stock solution bucket again, it is inside to get into the stock solution bucket at last, the straw is fixed with flexible section simultaneously. The subassembly is pressed down and is removed the section of stretching out and drawing back of pressing down, and the section of stretching out and drawing back compression drives the straw simultaneously and follows the axial downstream of stock solution bucket, presses down the subassembly and presses the section of stretching out and drawing back to different degrees, can make the end of straw reach the cell solution of different layers in the stock solution bucket in, through the vertical compression distance of the section of stretching out and drawing back of control to the displacement of control straw carries out PBMC and selects separately. After PBMC selects separately, press the subassembly rebound and leave flexible section gradually, the axial upward movement along the stock solution bucket is driven to the straw in the time of flexible section extension resumes, and the straw resumes initial uncompressed state under the effect of flexible section. Complete cell PBMC selects separately the process once with this completion to can be repeated many times carry out PBMC and select separately, through the cooperation of pressing subassembly and straw subassembly, replaced prior art's manual separation, improve and select separately efficiency and precision, avoid the external pollution to the cell solution of the in-process of selecting separately simultaneously.

In addition to the technical problems addressed by the present invention, the technical features of the constituent technical solutions, and the advantages brought by the technical features of these technical solutions, which have been described above, other technical features of the present invention and the advantages brought by these technical features will be further described with reference to the accompanying drawings, or can be learned by practice of the present invention.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

FIG. 1 is a schematic longitudinal sectional view of a resting state of a cell sorting device according to the present invention;

FIG. 2 is a schematic structural view of a sealing ring of the cell sorting device according to the present invention;

FIG. 3 is a schematic structural view of a resting state of the cell sorting device provided by the present invention;

FIG. 4 is a schematic longitudinal sectional view of a cell sorting device according to the present invention in a centrifugal state;

FIG. 5 is a schematic structural view of the centrifugal state of the cell sorting device according to the present invention;

FIG. 6 is a schematic structural view of a straw assembly of the cell sorting device according to the present invention;

FIG. 7 is a second schematic structural view of a straw assembly of the cell sorting device according to the present invention;

FIG. 8 is a third schematic structural view of a straw assembly of the cell sorting device according to the present invention;

fig. 9 is a schematic structural diagram of a connecting sleeve of the cell sorting device provided by the present invention;

FIG. 10 is a schematic structural diagram of a cell sorting and concentrating system provided by the present invention;

reference numerals:

100: a cell sorting device;

200: a supporting seat; 210: a liquid pipe is communicated; 220: a groove; 230: connecting sleeves; 240: a bearing; 250: a seal ring;

300: a liquid storage barrel; 310: a first through hole; 330: a third through hole; 340: a gas permeable membrane; 360: a first liquid layer; 370: a second liquid layer; 380: a third liquid layer;

400: a pressing assembly; 410: pressing a plate; 420: a pressure lever;

500: a straw assembly; 510: a straw; 520: a plug body; 530: a seal ring; 540: a third end plate; 550: a connecting rod; 521: a second through hole; 522: a telescopic section; 523: a fixed section; 5221: a telescoping member; 5222: a first end plate; 5224: a second end plate;

600: a pipeline; 700: a control device; 800: a support; 900: a liquid path channel.

Detailed Description

The following describes embodiments of the present invention in further detail with reference to the accompanying drawings and examples. The following examples are intended to illustrate the invention, but are not intended to limit the scope of the invention.

In the description of the embodiments of the present invention, it should be noted that the terms "central", "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of describing the embodiments of the present invention and simplifying the description, but do not indicate or imply that the designated device or element must have a specific orientation,

Constructed and operated in a particular orientation and therefore should not be construed as limiting the embodiments of the invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the embodiments of the present invention, it should be noted that, unless explicitly stated or limited otherwise, the terms "connected" and "connected" should be interpreted broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; may be directly connected or indirectly connected through an intermediate. The specific meaning of the above terms in the embodiments of the present invention can be understood in specific cases by those skilled in the art.

In embodiments of the invention, unless expressly stated or limited otherwise, the first feature may be directly on or directly under the second feature or indirectly via intermediate members. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of an embodiment of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.

As shown in fig. 1, fig. 3, fig. 4 and fig. 5, the embodiment of the present invention provides a cell sorting apparatus, including a liquid storage barrel 300, a straw assembly 500 and a pressing assembly 400, the straw assembly 500 includes a straw 510 and a plug body 520, the liquid storage barrel 300 is provided with a first through hole 310, the plug body 520 is disposed at the first through hole 310, the plug body 520 is configured with a second through hole 521 extending along the axial direction of the liquid storage barrel 300, the straw 510 passes through the second through hole 521 and enters the liquid storage barrel 300, the plug body 520 includes a telescopic section 522, one end of the telescopic section 522 is connected with the liquid storage barrel 300, the other end is fixedly connected with the straw 510, the pressing assembly 400 can drive the telescopic section 522 to be telescopic along the axial direction of the liquid storage barrel 300, so as to drive the straw 510 to move.

The utility model discloses cell sorting unit, the vertical placing of stock solution bucket 300, its inside cell solution that treats sorting of having the centrifugation layering of being equipped with, first through-hole 310 is seted up at the top of stock solution bucket 300, first through-hole 310 department installation cock body 520, cock body 520 constructs second through-hole 521 and stock solution bucket 300's inside intercommunication, cock body 520 has flexible section 522, and flexible section 522 is compressible or extension when receiving pressure, and the pressure disappears and stretches or contracts the reconversion. The stiff end of flexible section 522 is fixed on stock solution bucket 300, straw 510 passes second through-hole 521 and is fixed with the expansion end of flexible section 522, press the expansion end removal of subassembly 400 drive flexible section 522, make the whole compression of flexible section 522 or extension, thereby drive straw 510 along the axial downstream of stock solution bucket 300, press the subassembly 400 with flexible section 522 press to different degrees, can make the terminal of straw 510 arrive in the cell solution of stock solution bucket 300 different layers, through the vertical flexible distance of control flexible section 522, thereby control the displacement distance of straw 510, PBMC is selected separately. After the PBMC sorting is completed, the driving force of the pressing assembly 400 on the telescopic section 522 gradually disappears, the telescopic section 522 extends or contracts to recover and simultaneously drives the straw 510 to move upwards along the axial direction of the liquid storage barrel 300, and the straw 510 recovers to the initial position under the action of the telescopic section 522. Complete cell PBMC of once is selected separately with this process to can be repeated many times carry out PBMC and select separately, through the cooperation of pressing subassembly 400 and straw subassembly 500, replaced prior art's manual selection, improve and select separately efficiency and precision, avoid the external pollution to the cell solution of the in-process of selecting separately simultaneously.

In one embodiment, the telescopic member 522 may be located outside the barrel 300, i.e. the straw 510 passes through the second through hole 521 portion in the telescopic member 522, then passes through the second through hole 521 portion in the plug 520 located inside the barrel 300, and finally enters into the barrel 300, and the straw 510 is fixed to the telescopic member 522. The pressing assembly 400 moves downwards to press the telescopic section 522, the telescopic section 522 is compressed to simultaneously drive the straw 510 to move downwards along the axial direction of the liquid storage barrel 300, after PBMC sorting is completed, the pressing assembly 400 moves upwards to gradually leave the telescopic section 522, and the telescopic section 522 extends and recovers to simultaneously drive the straw 510 to move upwards along the axial direction of the liquid storage barrel 300.

In another embodiment, the telescopic segment 522 may also be located outside the barrel 300, i.e. the straw 510 first passes through the second through hole 521 portion of the plug body 520 located outside the barrel 300, then passes through the second through hole 521 portion of the telescopic segment 522, and finally enters into the barrel 300, and the straw 510 is fixed to the telescopic segment 522. The pressing assembly 400 moves downwards to press the telescopic section 522, the telescopic section 522 extends to simultaneously drive the straw 510 to move downwards along the axial direction of the liquid storage barrel 300, after PBMC sorting is completed, the pressing assembly 400 moves upwards to gradually leave the telescopic section 522, and the telescopic section 522 contracts and recovers to simultaneously drive the straw 510 to move upwards along the axial direction of the liquid storage barrel 300.

According to the utility model provides an embodiment, cock body 520 still includes canned paragraph 523, canned paragraph 523 and the coaxial setting of flexible section 522. In this embodiment, the axial direction of the whole plug body 520 is the same as the axial direction of the liquid storage barrel 300, and the telescopic section 522 and the fixed section 523 may form the plug body 520 in a sectional manner or form the plug body 520 in a nested manner. In the sectional type, when the telescopic segment 522 is located outside the liquid storage barrel 300, the plug body 520 sequentially comprises the telescopic segment 522 and the fixed segment 523 from outside to inside along the axial direction of the liquid storage barrel 300, and the pressing assembly 400 directly presses the telescopic segment 522 to drive the straw 510 to move; when the flexible section 522 is located the liquid storage barrel 300, the cock body 520 is the flexible section 522 and the fixed section 523 from inside to outside in proper order along the axial of the liquid storage barrel 300, and the pressing component 400 directly presses the fixed section 523 to drive the suction pipe 510 to move, thereby driving the flexible section 522 to stretch. Under the nested condition, the telescopic section 522 can be sleeved on the outer side of the part of the fixed section 523 located outside the liquid storage barrel 300, or can be sleeved on the outer side of the part of the fixed section 523 located inside the liquid storage barrel 300, and the pressing component 400 directly presses the fixed section 523 to drive the suction pipe 510 to move, so as to drive the telescopic section 522 to stretch and retract.

The telescopic segment 522 and the fixed segment 523 are connected into a whole, and the second through hole 521 jointly constructed in the telescopic segment and the fixed segment can be an equal-diameter through hole or a variable-diameter through hole, so that the straw 510 can smoothly pass through the through hole. The fixing segment 523 can stabilize the suction tube 510, ensure the sagging of the suction tube 510, and guide the movement of the suction tube 510.

According to an embodiment of the present invention, the extension section 522 includes an extension piece 5221 and a first end plate 5222, one end of the extension piece 5221 abuts against the liquid storage barrel 300, the other end is connected with the first end plate 5222, and the straw 510 passes through the first end plate 5222 and is fixedly connected with the first end plate 5222. In this embodiment, the retractable member 5221 may be a fully-enclosed retractable tubular structure, such as a bellows, a plastic tube, a spring, etc., or a fully-enclosed retractable connecting rod structure. When the telescopic section 522 is located outside the liquid storage barrel 300, the first end plate 5222 is fixed to the upper end of the telescopic piece 5221, the lower end of the telescopic piece 5221 abuts against the liquid storage barrel 300, and when the telescopic section 522 is located inside the liquid storage barrel 300, the first end plate 5222 is fixed to the lower end of the telescopic piece 5221, and the upper end of the telescopic piece is fixedly connected with the liquid storage barrel 300. The first end plate 5222 is provided with a mounting hole, the hollow portion of the tubular structure or the link structure and the through hole inside the fixed section 523 together form a second through hole 521 of the plug 520, and the suction pipe 510 is fixed at the mounting hole.

In this embodiment, the telescopic section 522 is located outside the liquid storage barrel 300, the pressing assembly 400 moves downward to press the first end plate 5222, the first end plate 5222 moves downward to drive the straw 510 to move downward, and the tubular structure or the connecting rod structure is compressed; the pressing assembly 400 is moved upward away from the first end plate 5222, and the tubular structure or the linkage structure is extended and restored, so that the first end plate 5222 is moved upward to move the suction pipe 510 back to the original position. In other embodiments, the telescopic section 522 is located inside the liquid storage barrel 300, the pressing assembly 400 moves down to press the fixing section 523, and the suction pipe 510 moves down to drive the first end plate 5222 to move down, so as to extend the tubular structure or the connecting rod structure; the pressing member 400 is moved upward away from the fixing portion 523, and the tubular structure or the link structure is retracted and restored, so that the first end plate 5222 is moved upward to move the suction pipe 510 back to the original position.

According to the utility model provides an embodiment, the canned paragraph 523 outside is located to extensible member 5221 cover, and canned paragraph 523 is connected with first terminal plate 5222. In this embodiment, the plug body 520 is formed by nesting the fixed section 523 and the telescopic section 522, the telescopic member 5221 is a spring, the spring is sleeved outside the fixed section 523, one end of the fixed section 523 is fixed to the first end plate 5222, in this embodiment, the telescopic section 522 is located outside the liquid storage barrel 300, the lower end of the spring is directly abutted to the top of the liquid storage barrel 300, when the pressing assembly 400 presses the first end plate 5222, the spring is compressed, and the first end plate 5222, the fixed section 523 and the suction pipe 510 are integrated and move downward synchronously; when the pressing assembly 400 moves upward and gradually moves away from the first end plate 5222, the spring is elastically expanded, the restoring force acts on the first end plate 5222, and the first end plate 5222, the fixing section 523 and the suction pipe 510 move upward synchronously. In other embodiments, the telescopic section 522 is located inside the liquid storage barrel 300, the upper end of the spring is directly abutted against the top of the liquid storage barrel 300, when the pressing assembly 400 presses the fixing section 523 downward, the spring stretches, and the first end plate 5222, the fixing section 523 and the suction pipe 510 are integrated and move downward synchronously; when the pressing member 400 moves upward and away from the fixed section 523, the spring is rebounded and contracted, the restoring force acts on the first end plate 5222, and the first end plate 5222, the fixed section 523 and the suction pipe 510 move upward synchronously.

As shown in fig. 2, according to an embodiment of the present invention, a sealing ring 530 is disposed between the fixing segment 523 and the first through hole 310. Under the condition that the fixed section 523 and the telescopic section 522 are in nested combination synchronous motion, good dynamic sealing performance between the first through hole 310 and the fixed section 523 needs to be ensured, so that the sealing ring 530 is arranged between the hole wall of the first through hole 310 and the outer wall of the fixed section 523 to realize sealing, and when the suction pipe 510 and the plug body 520 vertically move downwards, the gas and the liquid in the container are ensured to be in a completely sealed state and are not influenced by the external environment. In other embodiments, the sealing structure between the fixing segment 523 and the first through hole 310 is not limited to the sealing ring 530.

According to the utility model provides an embodiment, flexible section 522 includes annular second end plate 5224, and second end plate 5224's inboard is connected with canned paragraph 523, and an terminal surface and the extensible member 5221 of second end plate 5224 are connected, and another terminal surface and stock solution bucket 300 butt. As shown in fig. 6, the plug body 520 is formed by a plurality of segments of the telescopic segment 522 and the fixed segment 523, the telescopic member 5221 is a bellows, which is a fully sealed flexible structure and can be compressed or extended, and can also be restored to an uncompressed or non-extended state.

In this embodiment, the bellows is located the outside of liquid storage barrel 300, the fixed first end plate 5222 in upper end of bellows, the lower extreme of bellows sets up second end plate 5224, second end plate 5224 is the annular, the bottom surface and the liquid storage barrel 300 contact of second end plate 5224, the top surface is connected with the bellows, its inboard is connected with canned paragraph 523, canned paragraph 523 and second end plate 5224 can be integrated into one piece structure, second end plate 5224 extends first through-hole 310 to the outside turn-ups of liquid storage barrel 300 as canned paragraph 523 promptly. The mutual matching of the first end plate 5222, the expansion piece 5221, the second end plate 5224 and the fixing section 523 can ensure that the expansion piece 5221 expands and contracts to drive the straw 510 to move, and simultaneously, a good sealing effect between the plug body 520 and the first through hole 310 is ensured. When the pressing assembly 400 presses the first end plate 5222, the bellows is compressed, the fixed segment 523 is stationary, and the first end plate 5222 and the suction tube 510 are synchronously moved downward; as pressing assembly 400 moves upward and away from first end plate 5222, the bellows resiliently expands, and a restoring force acts on first end plate 5222, which first end plate 5222 and straw 510 move upward in unison.

In another embodiment, the corrugated tube is located inside the liquid storage barrel 300, the lower end of the corrugated tube fixes the first end plate 5222, the upper end of the corrugated tube is provided with the second end plate 5224, the second end plate 5224 is annular, the top surface of the second end plate 5224 contacts the liquid storage barrel 300, the bottom surface of the second end plate 5224 is connected with the corrugated tube, the inner side of the corrugated tube is connected with the fixing section 523, the fixing section 523 and the second end plate 5224 may be an integrally formed structure, that is, the second end plate 5224 is a flange of the fixing section 523 extending into the outside of the liquid storage barrel 300 to the first through hole 310. The mutual matching of the first end plate 5222, the expansion piece 5221, the second end plate 5224 and the fixing section 523 can ensure that the expansion piece 5221 expands and contracts to drive the straw 510 to move, and simultaneously, a good sealing effect between the plug body 520 and the first through hole 310 is ensured. When the pressing assembly 400 presses down the suction tube 510, the bellows is extended, the fixed segment 523 is stationary, and the first end plate 5222 and the suction tube 510 are simultaneously moved downward; as the pressing assembly 400 moves up and away from the straw 510, the bellows resiliently contracts, and a restoring force acts on the first end plate 5222, and the first end plate 5222 and the straw 510 move up in synchronization.

As shown in fig. 7 and 8, in one embodiment, the extension 5221 can also be a connecting rod, the upper end of which is fixed with a first end plate 5222, the lower end of which is provided with a second end plate 5224, the bottom surface of the second end plate 5224 is in contact with the liquid storage barrel 300, the top surface is connected with the beam na, and the inner side is connected with the fixing section 523. When the pressing assembly 400 presses the first end plate 5222 downward, the connecting rod is folded and compressed, the fixed section 523 is stationary, and the first end plate 5222 and the suction pipe 510 synchronously move downward; as the pressing assembly 400 moves upward and away from the first end plate 5222, the link is stretched and a restoring force acts on the first end plate 5222, and the first end plate 5222 and the suction pipe 510 move upward in synchronization. The liquid storage barrel 300 is internally sequentially divided into a first liquid layer 360, a second liquid layer 370 and a third liquid layer 380 from top to bottom, and along with the continuous downward movement of the pressing component 400, the pipe opening of the suction pipe 510 can sequentially reach the inside of the first liquid layer 360, the second liquid layer 370 and the third liquid layer 380, so that the three liquid layers can be respectively sorted. In other embodiments, the liquid stratification from top to bottom in the reservoir 300 can be three or more layers or two layers.

According to an embodiment of the present invention, the straw assembly 500 further comprises a third end plate 540 for contacting the pressing assembly 400, the third end plate 540 is disposed on the plug body 520, and a gap for the straw 510 to pass through is formed between the plug body 520 and the third end plate 540. In this embodiment, the third end plate 540 is further disposed above the plug body 520, when the telescopic section 522 is located outside the liquid storage barrel 300, the third end plate 540 is disposed coaxially with the first end plate 5222 and is connected to the first end plate 5222 through the connecting rod 550, a certain gap is formed between the first end plate 5222 and the second end plate 5224 by the length of the connecting rod 550, the suction pipe 510 enters the mounting hole of the first end plate 5222 through the gap, and the pressing component 400 contacts the third end plate 540 and presses the telescopic section 522, so that the pressing component 400 is effectively prevented from contacting the suction pipe 510, and the suction pipe 510 is squeezed to affect the subsequent sorting work. When the telescopic segment 522 is located inside the liquid storage barrel 300, the third end plate 540 is coaxially arranged with the fixed segment 523 and is connected through the connecting rod 550, a certain gap is formed between the upper end surface of the fixed segment 523 and the second end plate 5224 due to the length of the connecting rod 550, the suction pipe 510 enters the second through hole 521 of the fixed segment 523 through the gap, the pressing component 400 contacts the third end plate 540 and presses, so that the telescopic segment 522 is pressed, the pressing component 400 can be effectively prevented from contacting the suction pipe 510, and the suction pipe 510 is squeezed to influence the subsequent sorting work.

In one embodiment, the pressing assembly 400 may employ a pressing plate 410, a pressing rod 420, and a driver, wherein the pressing plate 410 is horizontally disposed, the driver is connected with the pressing plate 410 through the pressing rod 420, and the driver drives the pressing plate 410 to move up and down through the pressing rod 420 to act on the third end plate 540. The driver can adopt an air cylinder and other elements.

According to the utility model provides an embodiment, the utility model discloses cell sorting unit still includes supporting seat 200 and driving piece, and the supporting seat 200 level sets up, and stock solution bucket 300 rotates through the pivot with supporting seat 200 to be connected, and the pivot level sets up and just is connected with stock solution bucket 300's axial verticality, and the driving piece is connected with supporting seat 200 to drive supporting seat 200 around the rotation of axial verticality with the pivot.

The utility model discloses cell sorting unit still can realize the effect of centrifugal layering to cell solution, sets up corresponding mounting hole on supporting seat 200 and the stock solution bucket 300, and the mounting hole of supporting seat 200 is inserted to pivot one end, and the mounting hole of stock solution bucket 300 is inserted to the other end to this rotates stock solution bucket 300 and supporting seat 200 to be connected. The supporting seat 200 is horizontally arranged, and the liquid storage barrel 300 is vertically placed under the action of gravity when the supporting seat 200 is in a static state, namely the axial direction of the liquid storage barrel 300 is vertical to the horizontal direction of the supporting seat 200, and the axial direction of the rotating shaft is parallel to the horizontal direction of the supporting seat 200; the driving piece drives the supporting seat 200 to rotate in the horizontal direction, in the rotating process, the liquid storage barrel 300 rotates to the horizontal state gradually from the vertical state due to the inertia effect, the cell solution in the liquid storage barrel 300 starts to be centrifugally layered under the centrifugal force effect, the required cells are on the outermost layer, the impurities/culture medium are on the inner layer, namely, the cell solution is layered along the axial direction of the liquid storage barrel 300, and the cells are at the barrel bottom position of the liquid storage barrel 300; after the centrifugation is completed, the rotating speed of the driving piece driving the supporting seat 200 is slowly reduced, the liquid storage barrel 300 is also slowly rotated to the vertical state from the horizontal state to reset, the layering of the cell solution can still be kept along the axial layering of the liquid storage barrel 300, the required cells are on the lower layer, and the impurities/culture media are on the upper layer, namely, the cells are at the bottom of the liquid storage barrel 300. After the liquid storage barrel 300 is turned to the vertical state, the cells and the impurities/culture medium are kept layered, and the impurities/culture medium or the cells are discharged out of the liquid storage barrel 300 to be concentrated.

The utility model discloses a cell sorting unit passes through driving piece drive supporting seat 200 and rotates, stock solution bucket 300 is changeed to the horizontality by vertical state, cell solution to in the stock solution bucket 300 carries out centrifugal layering, can guarantee effective accurate centrifugal layering, angular acceleration through control driving piece motion, ensure that stock solution bucket 300 changes the in-process to vertical state by the horizontality, the cell is in the same place with impurity/culture medium unmixedly, the cell is in the barrel head position of stock solution bucket 300 all the time, turn to vertical state until stock solution bucket 300, cell solution is layering static in stock solution bucket 300, cell solution is concentrated in stock solution bucket 300 again, realize the concentration of cell solution under quiescent condition, need not to carry out the concentrated separation of cell under centrifugal state, reduce the cell damage, simple and convenient concentrated operation when simplifying the device structure. The liquid storage barrel 300 can be compatible with cell concentration of various volumes, and the volume of concentrated cell solution can reach more than 2L. The utility model discloses a totally closed full automatization's operation, compare in prior art can concentrated volume increase more than 3 times, the survival rate after the cell concentration has improved more than 10%.

In this embodiment, the driving member may be a motor, an output shaft of the motor is vertically connected to the supporting base 200, and the connection position is a rotation center of the supporting base 200. The motor can also be connected with a speed regulator of an adaptive model and the like to match the centrifugal requirement. In other embodiments, the driving member may also adopt other device structures capable of driving the supporting seat 200 to rotate, and is not limited by this embodiment.

In this embodiment, the top of stock solution bucket 300 sets up first through-hole 310, and inside straw 510 passed first through-hole 310 by the outside and inserted stock solution bucket 300, can be to the inside cell solution that pours into of stock solution bucket 300 into through straw 510, also can cultivate the suction with the inside cell of stock solution bucket 300 or impurity/, but also unified control of straw 510 of each stock solution bucket 300 single control, the concentrated operation of being convenient for. Guarantee the sealed back between the first through-hole 310 of liquid storage barrel 300 itself and liquid storage barrel 300 and straw 510, can realize the centrifugal layering and select separately or the concentration of the cell solution in totally closed environment, the cell solution is in liquid storage barrel 300 at centrifugal layering and select separately or concentrated in-process, avoids receiving external environment's pollution.

In order to ensure that the rotation of the liquid storage barrel 300 is not affected, the straw 510 should be made of a deformable material, such as a soft plastic pipe, a rubber pipe and the like, which can adapt to the rotation process of the liquid storage barrel 300, and when the liquid storage barrel 300 is changed between a horizontal state and a vertical state, the straw 510 can be deformed and bent to adapt to the position state of the liquid storage barrel 300.

According to the utility model provides an embodiment, supporting seat 200 is equipped with liquid pipe 210, straw 510 and liquid pipe 210 intercommunication. In this embodiment, the liquid flowing tube 210 is formed on the upper surface of the supporting base 200 and is integrally formed with the supporting base 200, that is, the axial direction of the liquid flowing tube 210 is perpendicular to the horizontal direction of the supporting base 200, the liquid flowing tube 210 is vertically upward in the static state of the supporting base 200, the part of the suction tube 510 located outside the liquid storage barrel 300 is communicated with the lower part of the liquid flowing tube 210, and when the driving member drives the supporting base 200 to rotate, the liquid flowing tube 210 and the suction tube 510 rotate synchronously. The cell solution can be injected through the upper end of the liquid passing tube 210, and the cells or impurities/culture substrates can be discharged.

As shown in fig. 9, according to the present invention, the liquid flowing tube 210 is coaxially disposed with the supporting seat 200, the upper end of the liquid flowing tube 210 is communicated with the pipeline 600 through the connecting sleeve 230, the bearing 240 for connecting with the pipeline 600 is disposed in the connecting sleeve 230, and the sealing ring 250 is disposed between the pipeline 600 and the inner wall of the connecting sleeve 230. In this embodiment, only one liquid flowing tube 210 is disposed on the support base 200, the axial direction of the liquid flowing tube 210 is the same as the rotation axial direction of the support base 200 driven by the driving member, and each suction tube 510 is communicated with the lower end of the liquid flowing tube 210. In other embodiments, a plurality of liquid passing tubes 210 can be arranged on the support base 200 in cooperation with the suction tubes 510, each liquid passing tube 210 corresponds to one suction tube 510, and the number of the liquid passing tubes 210 is selected according to actual needs.

In this embodiment, the lower end of the liquid flowing pipe 210 is integrally formed with the supporting base 200, the upper end is sleeved with one end of the connecting sleeve 230, the other end of the connecting sleeve 230 is provided with the bearing 240, the bearing 240 is connected with the pipeline 600 of the liquid supply device, the liquid of the liquid supply device is sent into the connecting sleeve 230 through the pipeline 600, and then enters the liquid flowing pipe 210 through the connecting sleeve 230, when the driving member drives the supporting base 200 to rotate, the liquid flowing pipe 210 rotates along the axial direction thereof to drive the connecting sleeve 230 to rotate, and the connecting sleeve 230 is rotatably connected with the pipeline 600 of the liquid supply device through the bearing 240, so that the pipeline 600 of the liquid supply device keeps still, thereby ensuring the liquid passage to be smooth and ensuring that the external liquid supply device does not influence the centrifugal operation process. The sealing ring 250 is arranged between the part of the pipeline 600 entering the connecting sleeve 230 and the connecting sleeve 230 to form a dynamic sealing structure at the position where the pipeline 600 is communicated with the liquid through pipe 210, so that the delivered liquid is ensured not to flow back and not to overflow the connecting sleeve 230. In other embodiments, the connection between the pipeline 600 and the liquid through pipe 210 may adopt different types of dynamic sealing structures, so that the dynamic sealing can be well ensured. The number and relative positions of the sealing rings 250 and the bearings 240 can be selected according to the actual structural requirements, and are not limited by the embodiment.

According to the utility model provides an embodiment, the upper portion of liquid storage bucket 300 on a lateral barrel wall of the rotatory axial who keeps away from supporting seat 200 is equipped with third through hole 330, and third through hole 330 communicates with the inside of liquid storage bucket 300, and third through hole 330 department is equipped with ventilated membrane 340. In this embodiment, in the centrifugation process, can produce the negative pressure in the liquid storage barrel 300, can't guarantee unblocked liquid circulation, consequently set up third through-hole 330 on liquid storage barrel 300 to set up ventilated membrane 340 and seal third through-hole 330, ventilated membrane 340 communicates with each other with external atmosphere, and the pressure value of guaranteeing the liquid storage barrel 300 inside and outside is the same, avoids appearing great pressure differential and leads to can't advance a kind and draw out the appearance. When the centrifugation is rotatory, stock solution bucket 300 rotates, and its one side bucket wall of keeping away from the rotatory axial of supporting seat 200 can be the state up, and the solution in third through-hole 330 position stock solution bucket 300 can not contact ventilated membrane 340, and under quiescent condition, the solution in stock solution bucket 300 can not reach third through-hole 330 contact ventilated membrane 340 yet, guarantees that ventilated membrane 340 can not meet water inefficacy.

According to an embodiment of the present invention, the supporting seat 200 is configured with a groove 220 axially recessed from the edge toward the rotation axis of the supporting seat 200, and the liquid storage barrel 300 is disposed in the groove 220. In this embodiment, the supporting seat 200 is sunken to form the recess 220 in the middle part of the supporting seat 200 in the edge, and in the liquid storage barrel 300 embedding recess 220, the relative both sides wall of recess 220 set up the mounting hole, correspond two pivots of connecting liquid storage barrel 300 of installation. The setting of recess 220 is guaranteeing that supporting seat 200 and liquid storage barrel 300 synchronous revolution carry out the concentrated basis of centrifugation on, can also reduce the space occupation volume of device, compact device structure.

In one embodiment, the plurality of liquid storage barrels 300 are provided, and the plurality of liquid storage barrels 300 are uniformly distributed around the rotation axis of the support base 200. In this embodiment, the number of the liquid storage barrels 300 is four, four grooves 220 and four suction pipes 510 are correspondingly arranged, and the four liquid storage barrels 300 are uniformly distributed around one liquid through pipe 210. When the driving member drives the supporting base 200 to rotate, the stress of the device can be ensured to be balanced, and the centrifugal force applied to each liquid storage barrel 300 is equal and balanced. In other embodiments, the number of the liquid storage barrels 300 can be selected according to actual needs, and is not limited by this embodiment.

As shown in fig. 10, the cell sorting and concentrating system provided by the embodiment of the present invention includes a control device 700, a liquid supply device and the cell sorting device 100 of the above embodiment, the liquid supply device and the driving member are all connected with the control device 700, the liquid supply device includes a support 800 and a liquid path channel 900, the support 800 is used for placing a liquid bag, and the liquid path channel 900 is communicated with the liquid storage barrel 300.

In the cell sorting and concentrating system of the embodiment of the present invention, the control device 700 has a software operation interface, which can perform man-machine interaction, control the start, stop and operation of the device, and is connected with the driving member of the cell sorting device 100, so as to control the rotation speed and acceleration of the driving member, and the start, stop and operation time of the driving member; the bracket 800 can be hung with a liquid bag filled with blood, buffer solution, separation solution and the like; the fluid passage 900 transfers the blood, the buffer solution, the separation solution, and other fluids placed on the rack 800 to the reservoir 300 in steps. After the operation procedure debugging in controlling means 700 is accomplished, through to controlling means 700 input parameter, liquid such as blood of automatic control support 800 flows into the stock solution bucket 300 of cell sorting unit 100 through liquid path passageway 900, then the rotation of control driving piece, thereby control the switching of stock solution bucket 300 between level and vertical state, make cell solution carry out the centrifugation layering under stock solution bucket 300 horizontal state, carry out static sorting or concentration under stock solution bucket 300 vertical state, can realize totally closed full automatic cell sorting or concentration, the manual sorting or concentration of having replaced prior art, avoid receiving external environment's influence, improve sorting or concentrated efficiency and precision.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention in its corresponding aspects.

Claims (13)

1. A cell sorting device characterized in that: including stock solution bucket, straw subassembly and pressing component, the straw subassembly includes straw and cock body, the stock solution bucket is equipped with first through-hole, the cock body set up in first through-hole department, the cock body structure is followed the second through-hole of the axial extension of stock solution bucket, the straw passes the second through-hole and gets into in the stock solution bucket, the cock body is including flexible section, the one end of flexible section with the stock solution bucket is connected, the other end with straw fixed connection, pressing component can drive flexible section is followed the axial direction of stock solution bucket is flexible, with the drive the straw removes.

2. The cell sorting device according to claim 1, wherein: the plug body still includes the canned paragraph, the canned paragraph with flexible section coaxial setting.

3. The cell sorting apparatus according to claim 2, wherein: the flexible section includes extensible member and first end plate, the one end of extensible member with the stock solution bucket butt, the other end with first end plate is connected, the straw passes first end plate and with first end plate fixed connection.

4. The cell sorting apparatus according to claim 3, wherein: the telescopic piece is sleeved on the outer side of the fixed section, and the fixed section is connected with the first end plate.

5. The cell sorting apparatus according to claim 4, wherein: and a sealing ring is arranged between the fixed section and the first through hole.

6. The cell sorting apparatus according to claim 3, wherein: the flexible section includes annular second end plate, the inboard of second end plate with the canned paragraph is connected, a terminal surface of second end plate with the extensible member is connected, another terminal surface with the stock solution bucket butt.

7. The cell sorting device according to claim 1, wherein: the suction pipe assembly further comprises a third end plate which is used for being in contact with the pressing assembly, the third end plate is arranged on the plug body, and a gap for the suction pipe to pass through is formed between the plug body and the third end plate.

8. The cell sorting device according to any one of claims 1 to 7, wherein: still include supporting seat and driving piece, the supporting seat level sets up, the stock solution bucket with the supporting seat rotates through the pivot to be connected, the pivot level set up and with the axial verticality of stock solution bucket, the driving piece with the supporting seat is connected, in order to drive the supporting seat around with the axial verticality's of pivot rotatory axial rotation.

9. The cell sorting apparatus according to claim 8, wherein: the supporting seat is provided with a liquid through pipe, and the suction pipe is communicated with the liquid through pipe.

10. The cell sorting apparatus according to claim 9, wherein: the liquid passing pipe is coaxially arranged with the supporting seat, the upper end of the liquid passing pipe is communicated with the pipeline through a connecting sleeve, a bearing connected with the pipeline is arranged in the connecting sleeve, and a sealing ring is arranged between the pipeline and the inner wall of the connecting sleeve.

11. The cell sorting apparatus according to claim 8, wherein: the upper portion of the liquid storage barrel, far away from one side barrel wall of the supporting seat in the rotating axial direction, is provided with a third through hole, the third through hole is communicated with the inside of the liquid storage barrel, and a breathable film is arranged at the position of the third through hole.

12. The cell sorting apparatus according to claim 8, wherein: the supporting seat is provided with a groove which is recessed from the edge to the rotating shaft of the supporting seat, and the liquid storage barrel is arranged in the groove.

13. A cell sorting concentration system, characterized in that: the cell sorting device comprises a control device, a liquid supply device and the cell sorting device as claimed in any one of claims 8 to 12, wherein the liquid supply device and the driving piece are connected with the control device, the liquid supply device comprises a support and a liquid path channel, the support is used for placing a liquid bag, and the liquid path channel is communicated with the liquid storage barrel.

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