CN215103319U

CN215103319U - Zebra fish embryo culture system

Info

Publication number: CN215103319U
Application number: CN202120612652.6U
Authority: CN
Inventors: 许强华; 祝凌月
Original assignee: Shanghai Ocean University
Current assignee: Shanghai Ocean University
Priority date: 2021-03-25
Filing date: 2021-03-25
Publication date: 2021-12-10
Anticipated expiration: 2031-03-25

Abstract

The utility model provides a zebra fish embryo cultivation system, which comprises a bottom plate with a rectangular structure, a support bracket and a cultivation device, wherein the support bracket is arranged at the upper end of the bottom plate, the cultivation device is arranged at the upper end of the support bracket, the cultivation device comprises a plurality of cultivation bins, an annular frame and a locking unit, the cultivation bins are uniformly arranged outside the annular frame in a surrounding way, and the locking unit is connected with the cultivation bins through the annular frame; the utility model discloses a breeding device is inside to be provided with a plurality of cultivation storehouses, simulates different environmental conditions through factors such as temperature, pressure in the different cultivation storehouses that change, and then can obtain multiunit data according to a plurality of cultivation storehouses, makes breeding device's experiment effectual, experiment accurate, is favorable to the follow-up use of experimental data; the utility model can accurately adjust the cultivating bin through the integral regulation and control of the locking unit, prevent the cultivating bin from falling off, and simultaneously facilitate the replacement and adjustment of the cultivating bin; the stable environment inside the cultivation bin is guaranteed through end cover sealing, and the accuracy of an experiment is guaranteed.

Description

Zebra fish embryo culture system

Technical Field

The utility model belongs to the technical field of zebra fish breeds, concretely relates to zebra fish embryo culture system.

Background

The zebra fish is elongated and slightly fusiform, has a small and slightly pointed head and a short tail, is full of a plurality of deep blue longitudinal stripes similar to zebra, and is organized compared with silvery white or golden yellow longitudinal stripes arranged alternately. The zebrafish and the human gene have high similarity of 87%, and the superiority of the zebrafish as a model organism is remarkable, which means that the experimental results are mostly suitable for the human body. The zebra fish can be used for monitoring the water quality environment.

At present, the existing zebra fish embryos generally have the following defects in the cultivation process: when the existing zebra fish embryos are subjected to experimental cultivation, the experiments cannot be performed by adopting multiple groups of data, so that the experimental effect is poor, the efficiency is low, and the experimental accuracy is influenced.

SUMMERY OF THE UTILITY MODEL

To the deficiency among the prior art, the utility model aims at providing a zebra fish embryo cultivation system.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

the utility model provides a zebra fish embryo cultivation system, is including the bottom plate, support bracket and the breeding device that are the rectangle structure, and the bottom plate lower extreme evenly is provided with the slipmat, and support bracket is installed to the bottom plate upper end, and breeding device is installed to support bracket upper end.

Wherein, breeding device includes a plurality of storehouses of breeding, annular frame and locking unit, breeds the storehouse and evenly encircles and install in the annular frame outside, and the locking unit is connected with breeding the storehouse through annular frame.

The locking unit includes the rotating turret, the dwang, the regulating plate, check lock lever and locking frame, and the dwang is installed through the bearing in annular frame upper end, and the rotating turret is installed to the dwang upper end, and the regulating plate is installed to the dwang lower extreme, and the regulating plate is located inside the annular frame, evenly is provided with the check lock lever on the regulating plate, and on the stop pin activity ran through the annular frame, the stop pin inboard was provided with the draw-in groove with check lock lever complex, and the stop pin outside is connected with the cultivation storehouse.

Further, breeding device still includes the fixed plate, the baffle, end cover and joint unit, the fixed plate is installed in the support bracket upper end, the baffle is evenly installed to the fixed plate upper end, be connected with the annular frame between the baffle inboard, annular frame fixed mounting is at fixed plate upper surface center, be provided with the dead slot between the adjacent baffle, be provided with the cultivation storehouse in the dead slot, annular frame middle part is hollow structure, annular frame mid-mounting has the locking unit, the end cover one end that can open and close is passed through the round pin hub connection and is put up the upper end at the annular, the end cover lid is breeding the storehouse upper end, the end cover outside is connected with cultivation storehouse outside through the joint unit.

Further, the joint unit includes axis of rotation, joint board and joint post, and the axis of rotation is installed to the end cover lateral wall, installs the joint board through the bearing in the axis of rotation, and the joint board lower extreme is the bayonet socket structure, cultivates the storehouse outside install with joint board complex joint post.

Further, evenly be provided with the recess between fixed plate and the cultivation storehouse, recess internally mounted has the hot plate, and the hot plate is inside evenly to be provided with the heating ring, and the heating ring is octagonal structure.

Furthermore, the cross section of the end cover is of a U-shaped structure, rubber sealing strips are uniformly arranged on the lower end face of the end cover, and a valve is arranged at the upper end of the end cover.

Further, cultivate the storehouse cross-section and be the trapezium structure, cultivate the storehouse middle part and be provided with the stock chest, cultivate the storehouse and make by transparent glass material, transparent glass material cultivate the storehouse can be convenient for the experimenter and observe.

Due to the adoption of the scheme, the beneficial effects of the utility model are that:

first, the utility model discloses an inside a plurality of storehouses of cultivating that are provided with of breeding device can change different environmental conditions of simulating factors such as temperature, pressure in cultivating the storehouse, and then can obtain multiunit data according to a plurality of storehouses of cultivating for breeding device's experiment is effectual, the experiment is accurate, does benefit to the follow-up use of experimental data.

Secondly, the cultivation device of the utility model can accurately adjust the cultivation bin through the integral regulation and control of the locking unit, thereby locking and fixing, preventing the cultivation bin from falling off, being beneficial to the cultivation bin to cultivate zebra fish embryos, being convenient for the replacement and adjustment of the cultivation bin and being beneficial to the use; can seal up cultivating the storehouse upper end through the end cover, guarantee to cultivate the inside stable environment that is in of storehouse, avoid taking place the phenomenon of gas leakage, guarantee the accuracy of experiment.

Drawings

Fig. 1 is a schematic structural diagram of the zebra fish embryo cultivation system of the present invention.

Fig. 2 is a schematic diagram of the cross-sectional structure of the zebra fish embryo cultivation system of the present invention.

Fig. 3 is a schematic structural diagram of the middle partition plate, the cultivation bin, the annular frame, the locking unit and the clamping column of the cultivation system of the present invention.

Reference numerals: 1-bottom plate, 2-support bracket, 3-breeding device, 31-fixed plate, 311-heating plate, 32-baffle, 33-breeding bin, 34-ring frame, 35-locking unit, 351-rotating frame, 352-rotating rod, 353-adjusting plate, 354-locking rod, 355-locking pin, 36-end cover, 37-clamping unit, 371-rotating shaft, 372-clamping plate and 373-clamping column.

Detailed Description

The utility model provides a zebra fish embryo culture system.

As shown in figures 1 to 3, the zebra fish embryo cultivation system of the utility model comprises a bottom plate 1 with a rectangular structure, a support bracket 2 and a cultivation device 3, wherein the lower end of the bottom plate 1 is evenly provided with an anti-slip mat, the support bracket 2 is installed at the upper end of the bottom plate 1, and the cultivation device 3 is installed at the upper end of the support bracket 2.

Wherein, the cultivating device 3 comprises a fixing plate 31, a partition plate 32, a plurality of cultivating bins 33, an annular frame 34, a locking unit 35, an end cover 36 and a clamping unit 37, the fixing plate 31 is installed at the upper end of the supporting bracket 2, the partition plate 32 is evenly installed at the upper end of the fixing plate 31, the annular frame 34 is connected between the inner sides of the partition plates 32, the annular frame 34 is fixedly installed at the center of the upper surface of the fixing plate 31, empty grooves are arranged between the adjacent partition plates 32, the cultivating bins 33 are arranged in the empty grooves, the cultivating bins 33 are evenly installed at the outer side of the annular frame 34 in a surrounding way, the middle part of the annular frame 34 is of a hollow structure, the locking unit 35 is installed at the middle part of the annular frame 34, the locking unit 35 is connected with the cultivating bins 33 through the annular frame 34, one end of the openable end cover 36 is connected to the upper end of the ring frame 34 through a pin shaft and enables the end cover 36 to rotate up and down, the end cover 36 covers the upper end of the cultivation bin 33 and is in sealing fit, and the outer side of the end cover 36 is connected with the outer side of the cultivation bin 33 through a clamping unit 37. During specific work, the zebra fish embryos are uniformly loaded into the cultivation bin 33 manually, then the cultivation bin 33 containing the embryo cells is uniformly placed in an empty groove between the partition plates 32, the inner side of the cultivation bin 33 is locked by adjusting the locking unit 35, the phenomenon that the cultivation bin 33 falls off is prevented, the end cover 36 can be hermetically connected with the cultivation bin 33 through the clamping unit 37, and the zebra fish embryos can be observed through adjusting temperature, pressure and the like.

The locking unit 35 comprises a rotating frame 351, a rotating rod 352, an adjusting plate 353, a locking rod 354 and a locking pin 355, wherein the rotating rod 352 is installed at the upper end of the annular frame 34 through a bearing, the rotating frame 351 is installed at the upper end of the rotating rod 352, the adjusting plate 353 is installed at the lower end of the rotating rod 352, the adjusting plate 353 is located inside the annular frame 34, the locking rod 354 is evenly arranged on the adjusting plate 353, the locking pin 355 movably penetrates through the annular frame 34, a clamping groove matched with the locking rod 354 is formed in the inner side of the locking pin 355, and the outer side of the locking pin 355 is connected with the cultivation bin 33. When the cultivation bin 33 is uniformly inserted into the empty groove, the adjusting plate 353 is manually driven to rotate through the rotating rod 352, the locking rod 354 at the upper end of the adjusting plate 353 is matched with the locking pin 355, and then the function of locking and fixing the inner side of the cultivation bin 33 can be realized.

As a preferred embodiment, the clamping unit 37 includes a rotation shaft 371, a clamping plate 372 and a clamping post 373, the rotation shaft 371 is installed on the outer side wall of the end cover 36, the clamping plate 372 is installed on the rotation shaft 371 through a bearing, the lower end of the clamping plate 372 is in a bayonet structure, and the clamping post 373 matched with the clamping plate 372 is installed on the outer side of the cultivation bin 33. During specific work, when the end cover 36 overturns downwards, the manual work is matched with the joint post 373 through rotating the joint plate 372, so that the joint plate 372 and the joint post 373 are clamped mutually, the sealing connection between the end cover 36 and the cultivation bin 33 is realized, and the observation of the subsequent experiment of the zebra fish embryos is facilitated.

As a preferred embodiment, a groove is uniformly formed between the fixing plate 31 and the incubation cavity 33, and a heating plate 311 is installed inside the groove. The inside heating ring that evenly is provided with of hot plate 311, the heating ring is octagonal structure, connects through the heat conduction pole looks electricity between the adjacent heating ring, is honeycomb type network structure between heating ring and the heat conduction pole and arranges, and the heating of the hot plate 311 of honeycomb type structure is effectual, the heating is even for hot plate 311 can heat cultivation storehouse 33 lower extreme uniformly. During specific work, the heating plate 311 can effectively heat the cultivation bin 33, and the growth conditions of the zebra fish embryos at different temperatures can be observed by adjusting different temperatures.

As a preferred embodiment, the cross section of the end cover 36 is in a U-shaped structure, rubber sealing strips are uniformly arranged on the lower end face of the end cover 36, and a valve is arranged at the upper end of the end cover 36. During specific work, the rubber sealing strips can effectively guarantee the connection tightness, the pressure between the end cover 36 and the cultivation bin 33 can be adjusted through the valve, and then the growth conditions of zebra fish embryos under different pressures can be observed.

As a preferred embodiment, cultivate the storehouse 33 cross-section and be the trapezium structure, cultivate the storehouse 33 middle part and be provided with the stock chest, cultivate the storehouse 33 and be made for transparent glass material, transparent glass material cultivate the storehouse 33 and can be convenient for the experimenter to observe.

The utility model discloses a cultivation system is at the use step of concrete during operation:

the first step is as follows: the zebra fish embryos are uniformly loaded into the cultivation bin 33 manually, and then the cultivation bin 33 with the zebra fish embryos is uniformly placed in the empty groove between the partition plates 32;

the second step is that: the adjusting plate 353 is manually driven to rotate through the rotating rod 352, and the locking rod 354 at the upper end of the adjusting plate 353 is matched with the locking pin 355, so that the function of locking and fixing the inner side of the cultivating bin 33 can be realized, and the phenomenon that the cultivating bin 33 falls off is prevented;

the third step: the end cover 36 can be hermetically connected with the cultivating bin 33 through the clamping unit 37, and the zebra fish embryo observation function is realized by adjusting temperature, pressure and the like.

The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. It will be readily apparent to those skilled in the art that various modifications to these embodiments and the generic principles defined herein may be applied to other embodiments without the use of the inventive faculty. Therefore, the present invention is not limited to the above-described embodiments. Those skilled in the art should appreciate that, in view of the principles of the present invention, modifications and variations may be made without departing from the scope of the invention.

Claims

1. The utility model provides a zebra fish embryo cultivation system, includes bottom plate (1), support bracket (2) and breeding device (3) that are the rectangle structure, its characterized in that: the anti-skid mat is uniformly arranged at the lower end of the bottom plate (1), a supporting bracket (2) is installed at the upper end of the bottom plate (1), and a cultivation device (3) is installed at the upper end of the supporting bracket (2);

the cultivation device (3) comprises a plurality of cultivation bins (33), an annular frame (34) and locking units (35), the cultivation bins (33) are uniformly arranged on the outer side of the annular frame (34) in a surrounding mode, and the locking units (35) are connected with the cultivation bins (33) through the annular frame (34);

locking unit (35) include rotating turret (351), dwang (352), regulating plate (353), check lock lever (354) and stop pin (355), dwang (352) are installed through the bearing in annular frame (34) upper end, rotating turret (351) are installed to dwang (352) upper end, regulating plate (353) are installed to dwang (352) lower extreme, regulating plate (353) are located inside annular frame (34), evenly be provided with check lock lever (354) on regulating plate (353), stop pin (355) activity runs through on annular frame (34), stop pin (355) inboard is provided with the draw-in groove with check lock lever (354) complex, stop pin (355) outside is connected with breeding storehouse (33).

2. The zebrafish embryo cultivation system of claim 1, wherein: the cultivating device (3) also comprises a fixing plate (31), a clapboard (32), an end cover (36) and a clamping unit (37), the fixing plate (31) is arranged at the upper end of the supporting bracket (2), the upper end of the fixing plate (31) is uniformly provided with a partition plate (32), the inner sides of the partition plates (32) are connected with an annular frame (34), the annular frame (34) is fixedly arranged at the center of the upper surface of the fixing plate (31), empty grooves are arranged between the adjacent partition plates (32), cultivation bins (33) are arranged in the empty grooves, the middle part of the annular frame (34) is of a hollow structure, the middle part of the annular frame (34) is provided with a locking unit (35), one end of an openable end cover (36) is connected with the upper end of the annular frame (34) through a pin shaft, the end cover (36) covers the upper end of the cultivation bin (33), and the outer side of the end cover (36) is connected with the outer side of the cultivation bin (33) through a clamping unit (37).

3. The zebrafish embryo cultivation system of claim 2, wherein: joint unit (37) include axis of rotation (371), joint board (372) and joint post (373), axis of rotation (371) is installed to end cover (36) lateral wall, joint board (372) is installed through the bearing in axis of rotation (371), joint board (372) lower extreme is the bayonet socket structure, cultivate storehouse (33) outside and install and joint post (373) with joint board (372) complex.

4. The zebrafish embryo cultivation system of claim 2, wherein: evenly be provided with the recess between fixed plate (31) and cultivation storehouse (33), recess internally mounted has hot plate (311).

5. The zebrafish embryo cultivation system of claim 4, wherein: heating plate (311) inside evenly is provided with the heating ring, and the heating ring is octagonal structure.

6. The zebrafish embryo cultivation system of claim 2, wherein: the cross section of the end cover (36) is of a U-shaped structure, rubber sealing strips are evenly arranged on the lower end face of the end cover (36), and a valve is arranged at the upper end of the end cover (36).

7. The zebrafish embryo cultivation system of claim 1, wherein: cultivate storehouse (33) cross-section and be the trapezium structure, it is provided with the stock chest to cultivate storehouse (33) middle part, it is made by transparent glass material to cultivate storehouse (33).