CN215103259U

CN215103259U - Cell disruption device for gene detection

Info

Publication number: CN215103259U
Application number: CN202120881363.6U
Authority: CN
Inventors: 李钊; 李潇
Original assignee: Shandong Yidian Gene Technology Co ltd
Current assignee: Shandong Yidian Gene Technology Co ltd
Priority date: 2021-04-27
Filing date: 2021-04-27
Publication date: 2021-12-10
Anticipated expiration: 2031-04-27

Abstract

The utility model discloses a gene detects uses cell disruption device relates to cell disruption device technical field. The utility model discloses a transducer, transducer's middle part is provided with the slip subassembly, one side fixedly connected with base of slip subassembly, and the slip subassembly drives the horizontal or vertical slip of base, first grip block and second grip block have been installed to the upside of base. The utility model discloses a set up first grip block and second grip block at the base upside, first grip block can press from both sides tightly fixedly to the container with the second grip block, reduce the appearance container when carrying out cell disruption and rock the unstable condition, thereby it is stable to have promoted cell disruption device, set up the slider in the channel, the plate body middle part sets up the spout, the slider slides along the spout and makes first grip block and second grip block relative motion, can adjust according to the container size of different specifications, thereby cell disruption device's practicality has been promoted.

Description

Cell disruption device for gene detection

Technical Field

The utility model belongs to the technical field of cell disruption device, especially, relate to a gene detects uses cell disruption device.

Background

Cell disruptors for disruption of the cell are currently the most commonly used disruption devices in bioproduction. Fermentation liquor for different strains. The effect of crushing is very different. The disruption method of the cell disruptor can be classified into two major types, mechanical method and non-mechanical method. Mechanical forces to which cells are subjected in mechanical disruption are mainly compressive and shear forces, while non-mechanical disruption mainly utilizes chemical or biochemical agents (e.g., enzymes) to alter the structure of cell walls or membranes and release intracellular material. According to the principle of the cell crusher, the cell crusher can be divided into an ultrasonic cell crusher, a high-pressure homogenate cell crusher and a high-speed bead milling cell crusher.

The patent application with publication number CN208395170U discloses a cell disruption device, including vibrating motor, vibrating motor has the vibration axle through the axle sleeve connection, the barrel has been cup jointed outward to the vibration axle, the vibration axle runs through outside barrel and its right-hand member stretch out the barrel, it has eccentric hammer A to peg graft in the left side of barrel to vibrate epaxial, be connected with spring A on the vibration axle between eccentric hammer A and the barrel, spring A bottom is connected with the bracing piece, the vibration axle bottom on barrel right side is connected with spring B, spring B's bottom also is connected with the bracing piece, it has eccentric hammer B to peg graft on the vibration axle on spring B right side, be provided with the broken ball of stereoplasm cell in the barrel. The utility model relates to a rationally, simple structure, the material receives the high impact with higher speed, surely rub, effect such as extrusion, cutting of medium in the barrel, can reach the crushing effect of ideal in the time of the utmost point weak point, and the material is the fluidization in crushing process, and the atress is even.

However, the device in the application is easy to shake in the container during use, has low stability, and is easy to spill out of the container when cells are broken.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a cell crushing device for gene testing has solved the aforesaid and has easily appeared rocking in the use container, and stability is not strong, easily spills the outer current problem of container during the cell breakage.

In order to achieve the purpose, the utility model is realized by the following technical proposal:

the utility model provides a cell disruption device for gene detection, includes the transducer, the middle part of transducer is provided with the slip subassembly, one side fixedly connected with base of slip subassembly, and the slip subassembly drives the base and transversely or vertical slip, first grip block and second grip block have been installed to the upside of base, the downside of first grip block and second grip block is provided with clamping device, and clamping device and first grip block, second grip block transmission are connected.

Optionally, the clamping device includes a fixed block, a fixed plate is fixed to one side of the fixed block, and a sliding block is fixed to one side of the fixed plate.

Optionally, clamping device still includes dwang, plate body, the one end of dwang is fixed with face gear, two spouts have been seted up at the middle part of plate body, the central point of plate body is fixed with the dead lever, one side of dead lever is fixed with the gear.

Optionally, the first clamping block and the second clamping block comprise arc-shaped blocks, and liner plates are fixed on the lower sides of the arc-shaped blocks.

Optionally, the sliding assembly includes a first sliding rail, and a second sliding rail is slidably fitted to one side of the first sliding rail.

Optionally, a channel is formed in the middle of the base, and an ultrasonic generator is installed on one side of the transducer.

The embodiment of the utility model has the following beneficial effect:

the utility model discloses an embodiment is through setting up first grip block and second grip block at the base upside, first grip block can press from both sides tightly fixedly to the container with the second grip block, reduce the appearance container when carrying out cell disruption and rock the unstable condition, thereby it is stable to have promoted cell disruption device, set up the slider in the channel, the plate body middle part sets up the spout, the slider slides along the spout and makes first grip block and second grip block relative motion, can adjust according to the container size of different specifications, thereby cell disruption device's practicality has been promoted.

Of course, it is not necessary for any particular product to achieve all of the above-described advantages at the same time.

Drawings

The accompanying drawings, which form a part of the present application, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention and not to limit the invention. In the drawings:

fig. 1 is a schematic view of an assembled three-dimensional structure of a transducer and an ultrasonic generator according to an embodiment of the present invention;

fig. 2 is a schematic view of an assembled cross-sectional structure of an ultrasonic generator and a sliding assembly according to an embodiment of the present invention;

FIG. 3 is a schematic view of the structure at A in FIG. 2;

FIG. 4 is a schematic sectional view of the cell disruption device according to an embodiment of the present invention;

fig. 5 is a schematic structural diagram at B in fig. 4.

Wherein the figures include the following reference numerals:

transducer 1, supersonic generator 2, sliding assembly 3, base 4, channel 5, fixed block 6, slider 7, plate body 8, spout 9, dead lever 10, gear 11, face gear 12, dwang 13, first grip block 14, second grip block 15, fixed plate 16, clamping device 17.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. The following description of at least one exemplary embodiment is merely illustrative in nature and is in no way intended to limit the invention, its application, or uses.

To maintain the following description of the embodiments of the present invention clear and concise, detailed descriptions of well-known functions and components may be omitted.

Referring to FIGS. 1 to 5, in the present embodiment, there is provided a cell disruption device for gene testing, comprising: transducer 1, the middle part of transducer 1 is provided with sliding assembly 3, one side fixedly connected with base 4 of sliding assembly 3 can be used to drive base 4 and remove, and sliding assembly 3 drives base 4 and transversely or vertical slip, first grip block 14 and second grip block 15 have been installed to the upside of base 4, can press from both sides tightly fixedly to the container, reduce the container when carrying out the cell disruption and rock unstable condition to it is stability to have promoted cell disruption device, first grip block 14 is provided with clamping device 17 with the downside of second grip block 15, is convenient for adjust first grip block 14, second grip block 15, and clamping device and first grip block 14, the transmission of second grip block 15 are connected, can adjust according to the container size of different specifications, thereby has promoted cell disruption device's practicality.

The application of one aspect of the embodiment is as follows: when using cell disruption device, dwang 13 among the clamping device 17 is rotated earlier, dwang 13 drives gear 11 and terminal gear 12 meshing, make gear 11 drive dead lever 10 and plate body 8 rotate, then slider 7 moves along spout 9, make slider 7 drive fixed plate 16 and fixed block 6 motion, then fixed block 6 drives first grip block 14 and second grip block 15 relative motion, it is fixed to make first grip block 14 and second grip block 15 adjust according to the container size of depositing the cell, at last start sliding assembly 3, make sliding assembly 3 drive base 4 and carry out position control, carry out the breakage to the cell, thereby the use to cell disruption device has been accomplished. It should be noted that all the electric devices referred to in this application may be powered by a storage battery or an external power source.

The clamping device 17 of this embodiment includes a fixing block 6, a fixing plate 16 is fixedly connected to one side of the fixing block 6, a slider 7 is fixedly connected to one side of the fixing plate 16, and the two fixing blocks 6 are respectively and fixedly connected to a first clamping block 14 and a second clamping block 15. The two fixing blocks 6 can drive the first clamping block 14 and the second clamping block 15 to move relatively to clamp and fix the container.

This embodiment clamping device 17 still includes dwang 13, plate body 8, the one end fixedly connected with face gear 12 of dwang 13, two spouts 9 have been seted up at the middle part of plate body 8, the central point fixedly connected with dead lever 10 of plate body 8, one side fixedly connected with gear 11 of dead lever 10, and slider 7 sliding fit is in spout 9. The rotating rod 13 is rotated, the rotating rod 13 drives the face gear 12 to be meshed with the gear 11, then the gear 11 drives the fixing rod 10 to rotate with the plate body 8, so that the sliding block 7 slides along the sliding groove 9 and simultaneously drives the fixing plate 16 to move relatively, and therefore the clamping device is used.

First grip block 14 of this embodiment includes the arc piece with second grip block 15, the downside fixedly connected with welt of arc piece, coincide from top to bottom two welts. The two lining plates are fixedly connected with the fixed block 6.

The sliding assembly 3 of this embodiment includes a first sliding rail, a second sliding rail is slidably fitted to one side of the first sliding rail, the first sliding rail and the second sliding rail slide vertically, and one side of the second sliding rail is fixedly connected to the base 4. The second slide rail slides along the first slide rail for adjusting the height of the base 4.

In the embodiment, the middle part of the base 4 is provided with a channel 5, one side of the transducer 1 is provided with an ultrasonic generator 2, a clamping device 17 is arranged in the channel 5, and the end face gear 12 is meshed with the gear 11. The clamping device 17 drives the first clamping block 14 and the second clamping block 15 to move relatively, so as to clamp and fix the container.

It should be noted that the terms "first," "second," and the like in the description and claims of this application and in the drawings described above are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used is interchangeable under appropriate circumstances such that the embodiments of the application described herein are capable of operation in sequences other than those illustrated or described herein.

In the description of the present invention, it should be understood that the orientation or positional relationship indicated by the orientation words such as "front, back, up, down, left, right", "horizontal, vertical, horizontal" and "top, bottom" etc. are usually based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplification of description, and in the case of not making a contrary explanation, these orientation words do not indicate and imply that the device or element referred to must have a specific orientation or be constructed and operated in a specific orientation, and therefore, should not be interpreted as limiting the scope of the present invention; the terms "inner and outer" refer to the inner and outer relative to the profile of the respective component itself.

Claims

1. A cell disruption device for gene detection, comprising: transducer (1), the middle part of transducer (1) is provided with slip subassembly (3), one side fixedly connected with base (4) of slip subassembly (3), and slip subassembly (3) drive base (4) and transversely or vertical slip, first grip block (14) and second grip block (15) have been installed to the upside of base (4), the downside of first grip block (14) and second grip block (15) is provided with clamping device (17), and clamping device is connected with first grip block (14), second grip block (15) transmission.

2. The cell disruption device for gene testing according to claim 1, wherein said clamping means (17) comprises a fixed block (6), a fixed plate (16) is fixed to one side of said fixed block (6), and a slider (7) is fixed to one side of said fixed plate (16).

3. The cell disruption device for gene detection according to claim 2, wherein the clamping device (17) further comprises a rotating rod (13) and a plate body (8), wherein an end face gear (12) is fixedly connected to one end of the rotating rod (13), two sliding grooves (9) are formed in the middle of the plate body (8), a fixing rod (10) is fixed to the central point of the plate body (8), and a gear (11) is fixed to one side of the fixing rod (10).

4. The cell disruption device for gene testing according to claim 3, wherein said first holding block (14) and said second holding block (15) comprise arc-shaped blocks, and a lining plate is fixed to the lower sides of said arc-shaped blocks.

5. The cell disruption device for gene testing according to claim 4, wherein said sliding member (3) comprises a first sliding rail, and a second sliding rail is slidably fitted to one side of said first sliding rail.

6. The cell disruption device for gene assay according to claim 5, wherein a channel (5) is formed in the middle of the base (4), and an ultrasonic generator (2) is installed on one side of the transducer (1).