CN215103174U

CN215103174U - Novel kit convenient for pretreatment before cell RNA extraction

Info

Publication number: CN215103174U
Application number: CN202120537195.9U
Authority: CN
Inventors: 徐银宁
Original assignee: Zhejiang Chinese Medicine University ZCMU
Current assignee: Zhejiang Chinese Medicine University ZCMU
Priority date: 2021-03-15
Filing date: 2021-03-15
Publication date: 2021-12-10
Anticipated expiration: 2031-03-15

Abstract

The utility model discloses a novel reagent box convenient for pretreatment before cell RNA extraction, the structure of which comprises a box body, an adjusting mechanism, a top cover, a handle, an auxiliary component, a clamping layer, a groove, a matching clamping layer, a spring and a clamping plate, wherein the lower end of the adjusting mechanism in the box body is arranged, air in the matching clamping layer can be pumped out to the outer end through an air inlet pipe when a piston reciprocates back and forth, and the connected area of the clamping layer and the matching clamping layer is in a vacuum state, thereby improving the quality of a placing space of a reagent tube, and the reagent box is simple to operate and convenient to implement; through having set up the auxiliary assembly at the top cap left end, the inseparability that the adjustable top cap of screw rod and box body are connected to the leakproofness when both connect with the tight layer of regulation clamp and cooperation clamp layer with this.

Description

Novel kit convenient for pretreatment before cell RNA extraction

Technical Field

The utility model relates to the technical field of medical equipment, concretely relates to novel kit of preliminary treatment before cell RNA draws of being convenient for.

Background

RNA is a single strand formed by transcription by taking a strand of DNA as a template and using a base complementary pairing principle, the main function is to realize the expression of genetic information on protein, the RNA is a bridge in the process of transmitting the genetic information, researchers or medical care personnel in the medical field often need to extract RNA from human blood leukocytes, and the kit can assist in the collection of the RNA.

The reagent box is storing the required reagent of extraction RNA, and the in-process of depositing the test tube in the reagent box still remains partial gas in its inside, when transporting or low temperature storage to the reagent box, reduces the effect of depositing to the test tube, and the compactness of the connection between box body top cap and the box body is adjusted the step comparatively loaded down with trivial details.

SUMMERY OF THE UTILITY MODEL

Technical problem to be solved

In order to overcome prior art not enough, present a novel kit of being convenient for cell RNA extraction pretreatment, solved the kit and in the required reagent of storage extraction RNA, the inherent in-process of depositing the test tube of kit, still remain partial gas in its inside, when transporting or low temperature storage to the kit, reduce the effect of depositing to the test tube, and the compactness of the connection between box body top cap and the box body adjusts the comparatively loaded down with trivial details problem of step.

(II) technical scheme

The utility model discloses a following technical scheme realizes: the utility model provides a novel reagent kit convenient for pretreatment before cell RNA extraction, which comprises a kit body, an adjusting mechanism, a top cover, a handle, an auxiliary component, a clamping layer, a groove, a matching clamping layer, a spring and a clamping plate, wherein the top of the kit body is provided with the top cover, the middle end of the top cover top is fixedly connected with the handle, the bottom of the top cover is fixedly connected with the clamping layer, the right end of the interior of the kit body is provided with the groove, the side surface of the groove is provided with the matching clamping layer, the left end of the interior of the kit body is fixedly connected with the spring, the spring is fixedly connected with the side surface of the clamping plate, the adjusting mechanism is in sliding connection with the bottom of the kit body, the auxiliary component is in rotating connection with the left end of the top cover, the adjusting mechanism comprises a circular plate, a sliding groove, a push rod, a reset spring, a piston, an air inlet pipe and an air outlet pipe, the circular plate is in sliding connection with the middle end of the bottom of the kit body, the sliding groove is arranged at the lower end of the interior of the kit body, the improved air inlet pipe is characterized in that the circular plate is fixedly connected with the front end of the push rod, a reset spring is arranged on the side face of the push rod, the front end of the reset spring is fixedly connected with the front end face of the sliding groove, the push rod drives the piston to be slidably connected with the rear end of the sliding groove, the air inlet pipe is communicated with the left end of the sliding groove, and the back end face of the sliding groove is communicated with the air outlet pipe.

Further, the auxiliary assembly comprises a rotating rod, a thread groove, a screw rod and a guide rod, the rotating rod is connected with the left end of the top cover top in a rotating mode, the left end of the box body is provided with the thread groove, the bottom of the rotating rod is coaxially rotated with the top of the screw rod, the thread groove is in threaded connection with the side face of the screw rod, the guide rod is fixedly connected with the right end of the bottom of the top cover, and the guide rod is connected with the right end of the box body in a sliding mode.

Furthermore, the push rod is vertically connected with the box body, and the push rod is of a cylindrical structure.

Further, the ratio of the length of the sliding chute to the length of the piston is 7: 3.

Furthermore, the air inlet pipe is of an L-shaped structure, and the left end of the air inlet pipe penetrates through the bottom end face of the groove.

Furthermore, the right end of the box body is provided with an auxiliary through hole matched with the guide rod.

Further, the screw and the guide rod are both located on the same X axis of the top cover.

Further, the spring material is the musical instrument steel wire.

Furthermore, the push rod is made of aluminum alloy.

(III) advantageous effects

Compared with the prior art, the utility model, following beneficial effect has:

1) a novel kit convenient to preliminary treatment before cell RNA draws, through having set up adjustment mechanism lower extreme in the box body, accessible intake pipe will cooperate when piston reciprocating motion to press from both sides tight intraformational air and take out to the outer end to make press from both sides tight layer and cooperate the region formation vacuum state that the tight layer is connected of clamp, improve the space quality of placing to reagent pipe, and easy operation, the implementation of being convenient for.

2) A novel kit convenient to preliminary treatment before cell RNA draws, through having set up the auxiliary assembly at the top cap left end, the tightness that the adjustable top cap of screw rod and box body are connected to adjust the leakproofness when pressing from both sides tight layer and cooperation tight layer and connecting with this.

Drawings

Other features, objects and advantages of the invention will become more apparent upon reading of the detailed description of non-limiting embodiments with reference to the following drawings:

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic structural view of the auxiliary assembly of the present invention;

FIG. 3 is a schematic view of the internal structure of the case body of the present invention;

FIG. 4 is a schematic view of the connection structure between the adjusting mechanism and the box body of the present invention;

fig. 5 is a schematic structural diagram of the adjusting mechanism of the present invention.

In the figure: the device comprises a box body-1, an adjusting mechanism-2, a top cover-3, a handle-4, an auxiliary component-5, a clamping layer-6, a groove-7, a matching clamping layer-8, a spring-9, a clamping plate-10, a circular plate-21, a sliding groove-22, a push rod-23, a return spring-24, a piston-25, an air inlet pipe-26, an air outlet pipe-27, a rotating rod-51, a thread groove-52, a screw rod-53 and a guide rod-54.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

Referring to fig. 1 and 2, the present invention provides a novel kit for pretreatment before extraction of cellular RNA: including box body 1, adjustment mechanism 2, top cap 3, handle 4, auxiliary assembly 5, press from both sides tight layer 6, recess 7, the tight layer 8 of cooperation clamp, spring 9 and

splint

10, 1 top of box body is equipped with top cap 3, 3 top middle-ends of top cap and 4 fixed connection of handle, 3 bottom fixedly connected with of top cap press from both sides tight layer 6, the right-hand member is equipped with recess 7 in the box body 1, recess 7 side is equipped with the tight layer 8 of cooperation clamp, left end and spring 9 fixed connection in the box body 1, spring 9 and splint 10 side fixed connection, adjustment mechanism 2 and 1 bottom sliding connection of box body, auxiliary assembly 5 rotates with 3 left ends of top cap to be connected.

Referring to fig. 3 and 4, the utility model provides a novel reagent kit for pretreatment before cell RNA extraction, the adjusting mechanism 2 comprises a circular plate 21, a chute 22, and a push rod 23, the air inlet pipe 26 is communicated with the left end of the sliding groove 22, the back end face of the sliding groove 22 is communicated with the air outlet pipe 27, the push rod 23 is vertically connected with the box body 1, the push rod 23 is of a cylindrical structure, the ratio of the length of the sliding groove 22 to the length of the piston 25 is 7:3, the air inlet pipe 26 is of an L-shaped structure, and the left end of the air inlet pipe 26 penetrates through the bottom end face of the groove 7.

Please refer to fig. 5, the utility model provides a novel kit convenient to preliminary treatment before cell RNA draws, auxiliary assembly 5 includes dwang 51, thread groove 52, screw rod 53 and guide bar 54, dwang 51 rotates with top cap 3 top left end to be connected, 1 left end of box body is equipped with thread groove 52, dwang 51 bottom and the coaxial rotation in screw rod 53 top, thread groove 52 and screw rod 53 side threaded connection, guide bar 54 and top cap 3 bottom right-hand member fixed connection,

guide bar

54 and 1 right-hand member sliding connection of box body, both screw rod 53 and guide bar 54 are located the same X of top cap 3 epaxially, stability when top cap 3 removes is improved, 1 right-hand member of box body is equipped with the auxiliary through-hole identical with guide bar 54, can provide the space for guide bar 54 joint.

The utility model provides a novel reagent kit which is convenient for pretreatment before cell RNA extraction through improvement, and the working principle is as follows;

firstly, when the equipment is used, the device is firstly placed in a working area, and then the equipment is connected with an external power supply, so that the required electric energy can be provided for the work of the equipment;

secondly, rotating the rotating rod 51 to drive the screw rod 53 to move in the threaded groove 52 and drive the top cover 3 to move upwards, so that the guide rod 54 moves at the right end of the box body 1, then placing reagent bottles with different types and specifications in the clamping plates 10 at two ends, clamping and fixing the reagent bottles by the clamping plates 10, and then placing the bagged RNase-free centrifuge tube and the bagged RNase-free cryopreservation tube into the upper end and the lower end of the matched clamping layer 8;

thirdly, the rotating rod 51 is reversely rotated to drive the top cover 3 to move downwards, the clamping layer 6 at the lower end of the top cover 3 is connected with and clamped by the matching clamping layer 8, the space in the clamping layer 6 is in a closed state, the rotating rod 51 is continuously rotated to drive the top cover 3 to drive the clamping layer 6 to move downwards, and the contact tightness between the clamping layer 6 and the matching clamping layer 8 is adjusted;

fourthly, when the air in the matching clamping layer 8 is extracted, thrust is applied to the circular plate 21, the circular plate 21 guides the thrust to the push rod 23, the push rod 23 stretches the return spring 24, the push rod 23 drives the piston 25 to compress the air in the chute 22 and transmit the air into the air outlet pipe 27 and then discharge the air to the outside, when the piston 25 is reset, the air in the matching clamping layer 8 can be extracted through the air inlet pipe 26 and transmitted to the front end of the chute 22, and the air in the matching clamping layer 8 is extracted by the air inlet pipe, so that the air in the matching clamping layer 8 can be in a vacuum state.

The utility model relates to a novel reagent box convenient to preliminary treatment before cell RNA extraction, through optimizing setting up adjustment mechanism 2 in box body 1 lower extreme, the piston 25 can be through intake pipe 26 when reciprocating forward and backward in the air in the cooperation clamping layer 8 take out to the outside end to make the region that clamping layer 6 and cooperation clamping layer 8 are connected form vacuum state, improve the space quality of placing the reagent pipe, and easy operation, the implementation of being convenient for; by providing the auxiliary member 5 at the left end of the top cover 3, the screw 53 can adjust the tightness of the connection of the top cover 3 to the case 1, and thus the tightness of the connection of the clamping layer 6 and the mating clamping layer 8.

Claims

1. A novel kit convenient for pretreatment before cell RNA extraction comprises a kit body (1) and a splint (10), wherein a top cover (3) is arranged at the top of the kit body (1);

the method is characterized in that: the novel box body structure is characterized by further comprising an adjusting mechanism (2) and an auxiliary component (5), wherein the adjusting mechanism (2) is connected with the bottom of the box body (1) in a sliding mode, the auxiliary component (5) is connected with the left end of the top cover (3) in a rotating mode, the adjusting mechanism (2) comprises a circular plate (21), a sliding groove (22), a push rod (23), a reset spring (24), a piston (25), an air inlet pipe (26) and an air outlet pipe (27), the circular plate (21) is connected with the middle end of the bottom of the box body (1) in a sliding mode, the sliding groove (22) is formed in the lower end of the box body (1), the circular plate (21) is fixedly connected with the front end of the push rod (23), the reset spring (24) is arranged on the side face of the push rod (23), the front end of the reset spring (24) is fixedly connected with the front end face of the sliding groove (22), the push rod (23) drives the piston (25) to be connected with the rear end of the sliding groove (22) in a sliding mode, and the air inlet pipe (26) is communicated with the left end of the sliding groove (22), the back end surface of the sliding chute (22) is communicated with the air outlet pipe (27).

2. The novel kit for facilitating pretreatment before extraction of cellular RNA according to claim 1, wherein: auxiliary assembly (5) are including dwang (51), thread groove (52), screw rod (53) and guide bar (54), dwang (51) are rotated with top cap (3) top left end and are connected, box body (1) left end is equipped with thread groove (52), dwang (51) bottom and the coaxial rotation in screw rod (53) top, thread groove (52) and screw rod (53) side threaded connection, guide bar (54) and top cap (3) bottom right-hand member fixed connection, guide bar (54) and box body (1) right-hand member sliding connection.

3. The novel kit for facilitating pretreatment before extraction of cellular RNA according to claim 1, wherein: top cap (3) top middle-end and handle (4) fixed connection, top cap (3) bottom fixedly connected with presss from both sides tight layer (6), the right-hand member is equipped with recess (7) in box body (1), recess (7) side is equipped with the cooperation and presss from both sides tight layer (8), left end and spring (9) fixed connection in box body (1), spring (9) and splint (10) side fixed connection.

4. The novel kit for facilitating pretreatment before extraction of cellular RNA according to claim 1, wherein: the push rod (23) is vertically connected with the box body (1), and the push rod (23) is of a cylindrical structure.

5. The novel kit for facilitating pretreatment before extraction of cellular RNA according to claim 1, wherein: the ratio of the length of the chute (22) to the length of the piston (25) is 7: 3.

6. The novel kit for facilitating pretreatment before extraction of cellular RNA according to claim 1, wherein: the air inlet pipe (26) is of an L-shaped structure, and the left end of the air inlet pipe (26) penetrates through the bottom end face of the groove (7).

7. The novel kit for facilitating pretreatment before extraction of cellular RNA according to claim 1, wherein: the right end of the box body (1) is provided with an auxiliary through hole matched with the guide rod (54).

8. The novel kit for facilitating pretreatment before extraction of cellular RNA according to claim 2, wherein: the screw rod (53) and the guide rod (54) are both positioned on the same X axis of the top cover (3).