CN215050224U - Cell digester is with digestion bottle that has tissue piece to hold back function - Google Patents

Abstract

The utility model provides a cell digester is with digestion bottle that has tissue piece and holds back function. The cell digester comprises a digestion bottle with a tissue block retaining function, wherein the digestion bottle comprises: the cell digester is provided with a feeding pipe, and the feeding pipe is provided with an interception mechanism, two fixing mechanisms and a closing mechanism; the intercepting mechanism comprises a filter sleeve, a digestion bottle and two clamping components, the filter sleeve is arranged in the feeding pipe, the top of the filter sleeve extends out of the feeding pipe, the digestion bottle is arranged in the filter sleeve, and the clamping components comprise Contraband-shaped blocks, a first spring, rectangular blocks, clamping blocks, circular blocks, strip-shaped blocks, a first connecting rod and a rotating handle. The utility model provides a cell digester can digest the tissue piece with the digestion bottle that has the tissue piece and hold back the function to can hold back remaining tissue piece after the digestion, avoid influencing the advantage of follow-up operation.

Description

Cell digester is with digestion bottle that has tissue piece to hold back function

Technical Field

The utility model belongs to the technical field of the cell digestion, especially, relate to a cell digester is with having digestion bottle that the function is held back to the tissue piece.

Background

In the process of preparing primary cells, tissues are required to be cut into small blocks, then digestive agents are adopted to digest the tissue blocks into single cells, and the cells are gradually separated from the upstream of the tissues to become single cell suspensions; the digestion chamber comprises a main body and a connecting part, the main body is a cubic cavity, the bottom of the main body is closed, the upper part of the main body is opened, and the connecting part is in a shape of a cone with the upper part being round and the lower part being gradually changed; the surge chamber includes bottle lid and sets up the dashpot at the bottle lid top, the bottle lid is cylindrical, the dashpot is cylindrical, and its diameter is greater than the bottle lid diameter, is equipped with the air vent on the bottle lid, the air vent runs through the dashpot, and its biological sample digestion bottle chooses for use different volumetric digestion bottles according to biological sample's volume, and the dashpot is connected in the digestion chamber top, prevents when shaking the digestion chamber, and has the digestive juice and the foam of corrosivity and spill at sample digestion in-process to facilities such as the incubator of placing the digestion bottle are not corroded in the protection, and its biological sample digestion bottle simple structure, the volume is clear and definite, and convenient control has the value of carrying out popularization and application.

However, the above-mentioned structure has a disadvantage that the device may not completely digest the tissue mass after digesting the tissue mass, and may affect the operation result if the remaining tissue mass is not trapped.

Therefore, there is a need to provide a new cell digester with a digestion bottle having a tissue mass trapping function to solve the above technical problems.

SUMMERY OF THE UTILITY MODEL

The utility model provides a technical problem provide one kind can digest the tissue piece to can hold back remaining tissue piece after the digestion, avoid influencing the cell digester of follow-up operation with having the digestion bottle that the function was held back to the tissue piece.

In order to solve the technical problem, the utility model provides a digestion bottle with the tissue block interception function for a cell digester, which comprises a cell digester, a cell collecting device and a cell collecting device, wherein the cell digester is provided with a feeding pipe, and the feeding pipe is provided with an interception mechanism, two fixing mechanisms and a closing mechanism;

the interception mechanism comprises a filter sleeve, a digestion bottle and two clamping components, the filter sleeve is arranged in the feeding pipe, the top of the filter sleeve extends out of the feeding pipe, the digestion bottle is arranged in the filter sleeve, the clamping components comprise Contraband-shaped blocks, a first spring, a rectangular block, a clamping block, a circular block, a strip-shaped block, a first connecting rod and a rotating handle, the Contraband-shaped block is fixedly arranged at the top of the filter sleeve, the first spring is fixedly arranged on the inner wall of one side of the Contraband-shaped block, the rectangular block is fixedly arranged at one end of the first spring, the clamping block is fixedly arranged on the outer wall of one side of the rectangular block, one side of the clamping block, which is far away from the rectangular block, is contacted with the digestion bottle, the circular block is rotatably arranged on the outer wall of one side of the Contraband-shaped block, the strip-shaped block is fixedly arranged on the circular block, and the first connecting rod is fixedly arranged on the outer wall of one side of the clamping block, the first connecting rod is connected with the strip-shaped block in a sliding mode, and the rotating handle is fixedly arranged on the circular block;

fixed establishment includes first recess, pull rod, fixed block and second spring, first recess is seted up on one side inner wall of inlet pipe, pull rod slidable mounting is in first recess, the one end of pull rod extends outside the inlet pipe, fixed block fixed mounting is in the one end of pull rod, outside one side of fixed block extends first recess, second spring sliding sleeve established on the pull rod, the one end of second spring and one side inner wall fixed connection of first recess, the other end of second spring and one side outer wall fixed connection of fixed block.

As a further proposal of the utility model, the closing mechanism comprises a first partition panel, a second partition panel, a pulling block, a support plate, a square rod, a first motor, a rotary disc, a second connecting rod and a third connecting rod, wherein the first partition panel is fixedly arranged in the feeding pipe, the second partition panel is slidably arranged in the feeding pipe, one side of the second partition panel extends out of the feeding pipe, the pulling block is fixedly arranged at one side of the second partition panel, the support plate is fixedly arranged at one side of the feeding pipe, the square rod is hinged at the top of the support plate, the first motor is fixedly arranged at the top of the support plate, the rotary disc is fixedly arranged on the output shaft of the first motor, the second connecting rod is fixedly arranged on the outer wall at one side of the rotary disc, the second connecting rod is slidably connected with the square rod, the third connecting rod is fixedly arranged on the outer wall at one side of the pulling block, the third connecting rod is connected with the square rod in a sliding mode.

As a further scheme of the utility model, first bar through-hole and second bar through-hole have been seted up on the square pole, the second connecting rod run through first bar through-hole and with first bar through-hole sliding connection, the third connecting rod run through second bar through-hole and with second bar through-hole sliding connection, a plurality of first unloading through-holes have been seted up on the first partition panel, a plurality of second unloading through-holes have been seted up on the second partition panel, and are a plurality of first unloading through-hole and a plurality of second unloading through-hole one-to-one.

As a further scheme of the utility model, fixed mounting has two spacing slide bars, two on one side inner wall of Contraband shape piece spacing slide bar all run through the rectangular block and with rectangular block sliding connection, two the equal fixed mounting of one end of spacing slide bar has spacing circular block.

As a further scheme of the utility model, two spacing recesses have been seted up in the first recess, two equal slidable mounting has spacing slider, two in the spacing recess one side that spacing slider is close to each other all with fixed block fixed connection.

As a further scheme of the utility model, the draw-in groove has been seted up to one side outer wall of filter sleeve, fixed block and draw-in groove looks adaptation, one side fixed mounting that the grip block kept away from the rectangular block has the slipmat.

Compared with the prior art, the utility model provides a cell digester has digestion bottle that the function was held back to the tissue piece has following beneficial effect:

1. the utility model can digest the tissue blocks by arranging the interception mechanism, and has the advantage of intercepting the residual tissue blocks after digestion;

2. the utility model has the advantages that the utility model is provided with the fixing mechanism, so that the digestion bottle can be fixed and suspended in the feeding pipe, and the lower storage space is reserved;

3. the utility model discloses a set up closing mechanism for can cut off the inlet pipe, and be convenient for digest the advantage of discharging the cell after the digestion into cell digester after finishing.

Drawings

In order to facilitate understanding for those skilled in the art, the present invention will be further described with reference to the accompanying drawings.

FIG. 1 is a schematic front sectional view of a digestion bottle with a tissue mass retention function for a cell digester according to the present invention;

FIG. 2 is an enlarged schematic view of portion A of FIG. 1;

FIG. 3 is an enlarged schematic view of portion B of FIG. 1;

fig. 4 is an enlarged schematic structural view of a portion C in fig. 1.

In the figure: 1. a cell digester; 2. a feed pipe; 3. a filter sleeve; 4. a digestion bottle; 5. contraband shaped blocks; 6. a first spring; 7. a rectangular block; 8. a clamping block; 9. a circular block; 10. a bar-shaped block; 11. a first connecting rod; 12. turning a handle; 13. a pull rod; 14. a fixed block; 15. a second spring; 16. a first partition panel; 17. a second partition panel; 18. pulling the block; 19. a support plate; 20. a square rod; 21. a first motor; 22. a turntable; 23. a second connecting rod; 24. and a third connecting rod.

Detailed Description

Please refer to fig. 1, fig. 2, fig. 3 and fig. 4, wherein fig. 1 is a schematic front sectional structural view of a digestion bottle with a tissue mass trapping function for a cell digester according to the present invention;

FIG. 2 is an enlarged schematic view of portion A of FIG. 1; FIG. 3 is an enlarged schematic view of portion B of FIG. 1; fig. 4 is an enlarged schematic structural view of a portion C in fig. 1. The cell digester comprises a digestion bottle with a tissue block retaining function, wherein the digestion bottle comprises: the cell digester 1 is provided with a feeding pipe 2, and the feeding pipe 2 is provided with an interception mechanism, two fixing mechanisms and a closing mechanism;

the interception mechanism comprises a filter sleeve 3, a digestion bottle 4 and two clamping components, the filter sleeve 3 is arranged in the feeding pipe 2, the top of the filter sleeve 3 extends out of the feeding pipe 2, the digestion bottle 4 is arranged in the filter sleeve 3, the clamping components comprise Contraband-shaped blocks 5, a first spring 6, a rectangular block 7, a clamping block 8, a circular block 9, a strip-shaped block 10, a first connecting rod 11 and a rotating handle 12, the Contraband-shaped block 5 is fixedly arranged at the top of the filter sleeve 3, the first spring 6 is fixedly arranged on the inner wall of one side of the Contraband-shaped block 5, the rectangular block 7 is fixedly arranged at one end of the first spring 6, the clamping block 8 is fixedly arranged on the outer wall of one side of the rectangular block 7, one side of the clamping block 8, which is far away from the rectangular block 7, is contacted with the digestion bottle 4, the circular block 9 is rotatably arranged on the outer wall of one side of the Contraband-shaped block 5, the strip-shaped block 10 is fixedly arranged on the circular block 9, the first connecting rod 11 is fixedly arranged on the outer wall of one side of the clamping block 8, the first connecting rod 11 is connected with the strip-shaped block 10 in a sliding manner, and the rotating handle 12 is fixedly arranged on the circular block 9;

fixed establishment includes first recess, pull rod 13, fixed block 14 and second spring 15, first recess is seted up on one side inner wall of inlet pipe 2, pull rod 13 slidable mounting is in first recess, the one end of pull rod 13 extends outside inlet pipe 2, 14 fixed mounting of fixed block is in the one end of pull rod 13, one side of fixed block 14 extends outside first recess, second spring 15 slip cap is established on pull rod 13, one side inner wall fixed connection of one end of second spring 15 and first recess, the other end of second spring 15 and one side outer wall fixed connection of fixed block 14.

As shown in fig. 4, the closing mechanism includes a first partition board 16, a second partition board 17, a pull block 18, a support board 19, a square rod 20, a first motor 21, a rotary table 22, a second connecting rod 23 and a third connecting rod 24, the first partition board 16 is fixedly installed in the feed pipe 2, the second partition board 17 is slidably installed in the feed pipe 2, one side of the second partition board 17 extends out of the feed pipe 2, the pull block 18 is fixedly installed at one side of the second partition board 17, the support board 19 is fixedly installed at one side of the feed pipe 2, the square rod 20 is hinged and installed at the top of the support board 19, the first motor 21 is fixedly installed at the top of the support board 19, the rotary table 22 is fixedly installed on an output shaft of the first motor 21, the second connecting rod 23 is fixedly installed on an outer wall at one side of the rotary table 22, the second connecting rod 23 is slidably connected with the square rod 20, the third connecting rod 24 is fixedly arranged on the outer wall of one side of the pulling block 18, and the third connecting rod 24 is in sliding connection with the square rod 20;

through setting up closing mechanism for can cut off inlet pipe 2, and be convenient for discharge the advantage into cell digester 1 with the cell after the digestion after the completion.

As shown in fig. 4, a first strip-shaped through hole and a second strip-shaped through hole are formed in the square rod 20, the second connecting rod 23 penetrates through the first strip-shaped through hole and is slidably connected with the first strip-shaped through hole, the third connecting rod 24 penetrates through the second strip-shaped through hole and is slidably connected with the second strip-shaped through hole, a plurality of first blanking through holes are formed in the first partition plate 16, a plurality of second blanking through holes are formed in the second partition plate 17, and the plurality of first blanking through holes and the plurality of second blanking through holes correspond to one another one by one;

by providing the first and second strip-shaped through holes, cells digested on the second partition plate 17 can be introduced into the cell digester 1.

As shown in fig. 2, two limiting slide bars are fixedly mounted on the inner wall of one side of the Contraband-shaped block 5, the two limiting slide bars penetrate through the rectangular block 7 and are in sliding connection with the rectangular block 7, and a limiting circular block is fixedly mounted at one end of each of the two limiting slide bars.

Under the mutual cooperation between spacing slide bar, rectangular block 7 and the spacing circular block, can prescribe a limit to the position that rectangular block 7 and grip block 8 extend to prevent that rectangular block 7 and grip block 8 from weighing down under the effect of gravity.

As shown in fig. 3, two limiting grooves are formed in the first groove, limiting sliding blocks are slidably mounted in the two limiting grooves, and one sides of the two limiting sliding blocks, which are close to each other, are fixedly connected with a fixed block 14;

through spacing recess, spacing slider and fixed block 14 between mutually supporting down, can extend to the length of inlet pipe 2 in to fixed block 14 spacing, prevent that fixed block 14 is whole to get into in the inlet pipe 2.

As shown in fig. 2, a clamping groove is formed in the outer wall of one side of the filter sleeve 3, the fixing block 14 is matched with the clamping groove, and an anti-slip pad is fixedly mounted on one side, far away from the rectangular block 7, of the clamping block 8;

through setting up the slipmat, can improve the frictional force between grip block 8 and digestion bottle 4, increase fixed effect.

The utility model provides a cell digester is with having digestion bottle that the function was held back to tissue piece's theory of operation as follows:

the first step is as follows: when the tissue block needs to be digested, the tissue block is placed into the digestion bottle 4, the digested cells move to the position above the second partition plate 17 through filtration of the filter sleeve 3, the first motor 21 is started, the first motor 21 drives the rotary table 22 to rotate, the feed pipe 2 drives the square rod 20 to move through the second connecting rod 23, the square rod 20 drives the pull block 18 to move through the third connecting rod 24, and when the plurality of first blanking through holes are positioned on the plurality of second blanking through holes and communicated with each other, the digested cells can be guided into the cell digester 1;

the second step is as follows: when needs clear up remaining tissue piece in the digestion bottle 4, outside pulling pull rod 13, pull rod 13 drives fixed block 14 and outwards removes, can take out filter sleeve 3 and digestion bottle 4 when fixed block 14 breaks away from the draw-in groove, take out the back, rotate and change 12, it rotates to 12 drive circular block 9, circular block 9 drives strip-shaped block 10 and removes, strip-shaped block 10 drives the removal of grip block 8 through first connecting rod 11, when grip block 8 breaks away from digestion bottle 4, can separate filter sleeve 3 and digestion bottle 4, thereby clear up remaining tissue piece in filter sleeve 3.

It should be noted that the device structure and the accompanying drawings of the present invention mainly describe the principle of the present invention, and in the design principle, the settings of the power mechanism, the power supply system, the control system, etc. of the device are not completely described, and on the premise that the skilled person understands the principle of the present invention, the details of the power mechanism, the power supply system, and the control system can be clearly known, the control mode of the application file is automatically controlled by the controller, and the control circuit of the controller can be realized by simple programming of the skilled person in the art;

the standard parts used in the method can be purchased from the market, and can be customized according to the description of the specification and the accompanying drawings, the specific connection mode of each part adopts conventional means such as mature bolts, rivets, welding and the like in the prior art, the machines, parts and equipment adopt conventional models in the prior art, and the structure and the principle of the parts known by the skilled person can be known by technical manuals or conventional experimental methods.

Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, and it is intended that the scope of the invention be defined by the appended claims and their equivalents and that all such modifications are intended to be encompassed by the scope of the invention as defined in the claims.

Claims (6)

1. A digestion bottle with a tissue block retaining function for a cell digester is characterized by comprising:

the cell digester is provided with a feeding pipe, and the feeding pipe is provided with an interception mechanism, two fixing mechanisms and a closing mechanism;

the interception mechanism comprises a filter sleeve, a digestion bottle and two clamping components, the filter sleeve is arranged in the feeding pipe, the top of the filter sleeve extends out of the feeding pipe, the digestion bottle is arranged in the filter sleeve, the clamping components comprise Contraband-shaped blocks, a first spring, a rectangular block, a clamping block, a circular block, a strip-shaped block, a first connecting rod and a rotating handle, the Contraband-shaped block is fixedly arranged at the top of the filter sleeve, the first spring is fixedly arranged on the inner wall of one side of the Contraband-shaped block, the rectangular block is fixedly arranged at one end of the first spring, the clamping block is fixedly arranged on the outer wall of one side of the rectangular block, one side of the clamping block, which is far away from the rectangular block, is contacted with the digestion bottle, the circular block is rotatably arranged on the outer wall of one side of the Contraband-shaped block, the strip-shaped block is fixedly arranged on the circular block, and the first connecting rod is fixedly arranged on the outer wall of one side of the clamping block, the first connecting rod is connected with the strip-shaped block in a sliding mode, and the rotating handle is fixedly arranged on the circular block;

fixed establishment includes first recess, pull rod, fixed block and second spring, first recess is seted up on one side inner wall of inlet pipe, pull rod slidable mounting is in first recess, the one end of pull rod extends outside the inlet pipe, fixed block fixed mounting is in the one end of pull rod, outside one side of fixed block extends first recess, second spring sliding sleeve established on the pull rod, the one end of second spring and one side inner wall fixed connection of first recess, the other end of second spring and one side outer wall fixed connection of fixed block.

2. The cell digester as claimed in claim 1, having a tissue mass retaining function, wherein: the closing mechanism comprises a first partition board, a second partition board, a pull block, a support board, a square rod, a first motor, a rotary table, a second connecting rod and a third connecting rod, the first partition plate is fixedly arranged in the feeding pipe, the second partition plate is arranged in the feeding pipe in a sliding way, one side of the second partition plate extends out of the feeding pipe, the pulling block is fixedly arranged on one side of the second partition plate, the supporting plate is fixedly arranged on one side of the feeding pipe, the square rod is hinged and arranged on the top of the supporting plate, the first motor is fixedly arranged at the top of the supporting plate, the turntable is fixedly arranged on an output shaft of the first motor, the second connecting rod is fixedly arranged on the outer wall of one side of the turntable and is connected with the square rod in a sliding way, the third connecting rod is fixedly installed on the outer wall of one side of the pulling block and is in sliding connection with the square rod.

3. The cell digester as claimed in claim 2, having a tissue mass retaining function, wherein: the square pole is last to have seted up first bar through-hole and second bar through-hole, the second connecting rod run through first bar through-hole and with first bar through-hole sliding connection, the third connecting rod run through second bar through-hole and with second bar through-hole sliding connection, a plurality of first unloading through-holes have been seted up on the first partition panel, a plurality of second unloading through-holes have been seted up on the second partition panel, and are a plurality of first unloading through-hole and a plurality of second unloading through-hole one-to-one.

4. The cell digester as claimed in claim 1, having a tissue mass retaining function, wherein: and two limiting slide bars are fixedly mounted on the inner wall of one side of the Contraband-shaped block, the two limiting slide bars penetrate through the rectangular block and are in sliding connection with the rectangular block, and one ends of the two limiting slide bars are fixedly mounted with limiting round blocks.

5. The cell digester as claimed in claim 1, having a tissue mass retaining function, wherein: two limiting grooves are formed in the first groove, two limiting sliding blocks are arranged in the limiting grooves in a sliding mode, and one sides, close to each other, of the limiting sliding blocks are fixedly connected with the fixing blocks.

6. The cell digester as claimed in claim 1, having a tissue mass retaining function, wherein: the clamping groove is formed in the outer wall of one side of the filter sleeve, the fixing block is matched with the clamping groove, and the anti-slip pad is fixedly mounted on one side, far away from the rectangular block, of the clamping block.

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