CN215030074U

CN215030074U - Intelligent gradient liquid separation system

Info

Publication number: CN215030074U
Application number: CN202121277069.0U
Authority: CN
Inventors: 万杰
Original assignee: Chongqing Sichengrui Technology Co ltd
Current assignee: Chongqing Sichengrui Technology Co ltd
Priority date: 2021-06-07
Filing date: 2021-06-07
Publication date: 2021-12-07
Anticipated expiration: 2031-06-07

Abstract

The utility model belongs to the technical field of gradient liquid separation, and discloses an intelligent gradient liquid separation system, which solves the problems of time consumption, poor repeatability, separation zone disturbance and large error caused by the prior art; the device comprises a main machine body, wherein an electric pipette arm is arranged on the lower portion of one side of the main machine body, fraction collecting sample tubes are arranged on the front side of the electric pipette arm and penetrate through the top and the bottom of the main machine body, a centrifuge tube rack bracket is arranged on the lower portion of the main machine body and is positioned below the electric pipette arm, a centrifuge tube rack is arranged at the top end of the centrifuge tube rack bracket, an ultracentrifuge tube bracket is arranged on the bottom of the other side of the main machine body, and an ultracentrifuge tube is arranged in the ultracentrifuge tube bracket and penetrates through the middle portion of the top end of the ultracentrifuge tube bracket; the automatic separation and collection device has the advantages of achieving automatic separation and automatic collection, reducing errors, being high in repeatability, saving time and avoiding disturbance of separation zones.

Description

Intelligent gradient liquid separation system

Technical Field

The utility model relates to a gradient liquid separation technical field specifically indicates an intelligence gradient liquid separation system.

Background

During density gradient centrifugation, the sedimentation coefficient of sample particles with large concentration is large, downward sedimentation is fast, the sedimentation coefficient of sample particles with small concentration is small, and downward sedimentation is slow; with the prolonging of the centrifugation time, each sample particle stays at the position of the gradient liquid with the same concentration as the sample particle and does not move downwards, finally, the mixed sample is gradually separated in the gradient liquid, and the samples with various particle sizes are layered in the gradient liquid.

The prior empirical methods include the following:

1. cutting method: firstly putting the centrifuged centrifugal tube and the sample in the tube into a refrigerator for quick freezing, then cutting the frozen plastic tube into slices by using a centrifugal tube cutter, and taking out the samples layer by layer.

2. Puncture method: the bottom of the centrifuge tube is punctured by a needle, so that the gradient freely drips out by gravity, and then the gradients are sequentially distributed and collected.

3. Siphoning: a polyethylene small tube is inserted into the bottom of the tube, and the gradient liquid is sucked out by a siphon method.

Although the three methods described above can perform normal gradient liquid separation work, there are the following problems:

1. cutting method: time consuming and many thick walled or metal tubes are not suitable.

2. Puncture method: the operation of the centrifugal tube can not be used, is not suitable for recovering the gradient of sediment at the bottom of the tube, and is also not suitable for stainless steel or thick-wall plastic tubes.

3. Siphoning: the method causes the separation zone to be disturbed;

4. the manual participation degree is too high in the using process, and the error is large.

SUMMERY OF THE UTILITY MODEL

The utility model provides an intelligence gradient liquid separation system has solved the time-consuming that mentions among the prior art, has repeated relatively poor, arouse that the separation zone disturbs and the great problem of error.

The technical scheme of the utility model is realized like this: an intelligent gradient liquid separation system comprises a host body, wherein an electric transfer arm is installed on the lower portion of one side of the host body, a fraction collecting sample tube is installed on the front side of the electric transfer arm through the top and the bottom, a centrifuge tube rack bracket is installed on the lower portion of the host body below the electric transfer arm, a centrifuge tube rack is installed on the top end of the centrifuge tube rack bracket, an overspeed centrifuge tube bracket is installed on the bottom of the other side of the host body, an overspeed centrifuge tube is installed in the middle of the inner through top end of the overspeed centrifuge tube bracket, a photographing window is arranged on the lower portion of the other side of the host body corresponding to the position of the overspeed centrifuge tube, a camera is installed at the rear end of the photographing window, a horn mouth is arranged on the middle portion of the other side of the host body right above the overspeed centrifuge tube, and an electric push rod is connected between the top end of the horn mouth and the middle portion of the other side of the host body, the utility model discloses a quick-witted with quick-witted body, including host computer body, sample tube, test tube, and.

Preferably, move between the liquid arm and the host computer body through ball and be connected, ball includes swivel nut, circulator and lead screw, the swivel nut is installed at electronic liquid arm rear end that moves, swivel nut internally mounted has the circulator, the lead screw is installed in host computer body one side lower part position department, lead screw one end has the rotation motor through retarder connection, through ball's removal to move the removal of liquid arm electronically.

Preferably, the centrifuge tube rack bracket comprises a fixed support, a movable support and a buckle, the outer side of the fixed support is connected with the movable support through the buckle, and the movable support is changed to the position of the fixed support through the buckle so as to adjust the height; spacing holes are evenly formed in the outer side of the movable support, limiting rods are installed in the spacing holes, and the limiting rods adapt to centrifuge tube racks of different sizes through the positions of the spacing holes in different places.

Preferably, host computer body front end bottom articulates there is the host computer qianmen to in protection, host computer body front end mid-mounting has power button, the power control of being convenient for, touch display screen has been inlayed to host computer body front end upper portion, so that show.

Preferably, install the draw-in groove between overspeed centrifuge tube support bottom and the host computer body opposite side bottom, the bayonet socket has been seted up corresponding to draw-in groove position department in overspeed centrifuge tube support bottom to the installation of overspeed centrifuge tube support at the draw-in groove.

Preferably, transparent protection piece is installed to the window inboard of shooing, and the fixed glue has been cup jointed in the transparent protection piece outside, prevents that liquid from spilling, pollutes the camera lens, the subsequent normal use of being not convenient for.

The utility model has the advantages that:

1. the electric push rod drives the bell mouth to extrude gradient liquid in the ultracentrifuge tube, and the fraction collecting sample tube flows out of the tube orifice of the electric pipette arm into the centrifuge tube on the centrifuge tube rack through the ultraviolet detector flow cell, so that automatic separation and automatic collection are realized, errors are reduced, the repeatability is high, the time is saved, and the disturbance of a separation zone is avoided;

2. the method can also provide ultraviolet detection while separating gradient liquid, carry out ultraviolet absorption drawing on a flowing sample in the whole separation process, truly reflect the ultraviolet absorption condition of the sample in each collecting pipe, and correspondingly correspond the sample collecting pipes to corresponding positions on an ultraviolet absorption map one by one;

3. through carrying out imaging processing and directly perceived show on touch display screen to gradient liquid in the ultracentrifuge tube, make things convenient for the operator to set up the separation action according to oneself demand, acquire the strip that oneself wanted.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

Fig. 1 is a schematic view of the overall structure of the present invention;

fig. 2 is the utility model discloses the mounting structure schematic diagram of LED lamp pearl.

In the figure: 1. a host body; 2. fraction collection sample tube; 3. an electric pipetting arm; 4. a centrifuge tube rack; 5. a centrifuge tube rack bracket; 6. a host front door; 7. a card slot; 8. an ultracentrifuge tube rack; 9. an ultracentrifuge tube; 10. a photographing window; 11. a bell mouth; 12. a power button; 13. a touch display screen; 14. a camera; 15. a weak light plate; 16. LED lamp beads; 17. a light-condensing and light-reflecting plate; 18. an ultraviolet detector flow cell.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-2, an intelligent gradient liquid separation system comprises a main body 1, an electric transfer arm 3 is installed on the lower portion of one side of the main body 1, a fraction collecting sample tube 2 is installed on the front side of the electric transfer arm 3 through the top and bottom ends, a centrifuge tube rack bracket 5 is installed on the lower portion of the main body 1 at the position below the electric transfer arm 3, a centrifuge tube rack 4 is installed on the top end of the centrifuge tube rack bracket 5, an overspeed centrifuge tube bracket 8 is installed on the bottom portion of the other side of the main body 1, an overspeed centrifuge tube 9 is installed in the overspeed centrifuge tube bracket 8 through the middle portion of the top end, a photographing window 10 is arranged on the lower portion of the other side of the main body 1 corresponding to the position of the overspeed centrifuge tube 9, a camera 14 is installed on the rear end of the photographing window 10, a horn mouth 11 is arranged on the position above the overspeed centrifuge tube 9 in the middle portion of the other side of the main body 1, and an electric push rod is connected between the top end of the horn mouth 11 and the middle portion of the other side of the main body 1, one end of a fraction collecting sample tube 2, which is far away from an electric pipetting arm 3, penetrates through an ultraviolet detector flow cell 18 to be connected with the inner side of a bell mouth 11, a weak light plate 15 is fixed at the front end of the inner side of an overspeed centrifuge tube support 8, a light-gathering light-reflecting plate 17 is installed at the bottom end of the overspeed centrifuge tube support 8, which penetrates through a clamping groove 7 and the outer side of a main machine body 1, LED lamp beads 16 are installed at the gap between the weak light plate 15 and the overspeed centrifuge tube support 8 and at the bottom of the light-gathering light-reflecting plate 17, an electric push rod drives the bell mouth 11 to squeeze gradient liquid in the overspeed centrifuge tube 9, the fraction collecting sample tube 2 flows out from a tube opening at the electric pipetting arm 3 through the ultraviolet detector flow cell 18 to a centrifuge tube on a centrifuge tube support 4 to realize automatic separation and automatic collection, and ultraviolet detection can be provided while separating the gradient liquid, ultraviolet absorption and drawing are carried out on a flowing sample in the whole separation process, so as to truly reflect the ultraviolet absorption condition of the sample in each collecting tube, and the sample collection tubes are corresponding to the corresponding positions on the ultraviolet absorption spectrum one by one.

Referring to fig. 1-2, the electric liquid-transfering arm 3 is connected with the main body 1 through a ball screw, the ball screw comprises a screw sleeve, a circulator and a screw rod, the screw sleeve is installed at the rear end of the electric liquid-transfering arm 3, the circulator is installed in the screw sleeve, the screw rod is installed at the lower position of one side of the main body 1, one end of the screw rod is connected with a rotating motor through a reducer, and the movement of the electric liquid-transfering arm 3 is facilitated through the movement of the ball screw; the centrifuge tube rack bracket 5 comprises a fixed bracket, a movable bracket and a buckle, wherein the outer side of the fixed bracket is connected with the movable bracket through the buckle, and the movable bracket is changed at the position of the fixed bracket through the buckle so as to adjust the height; limiting holes are uniformly formed in the outer side of the movable support, limiting rods are arranged in the limiting holes, and the limiting rods adapt to centrifuge tube racks 4 with different sizes through the positions of the limiting holes in different places; the bottom of the front end of the main machine body 1 is hinged with a main machine front door 6 to facilitate protection, a power button 12 is arranged in the middle of the front end of the main machine body 1 to facilitate power control, a touch display screen 13 is embedded on the upper portion of the front end of the main machine body 1 to facilitate display, and gradient liquid in the overspeed centrifuge tube 9 is subjected to imaging processing and is visually displayed on the touch display screen 13 to facilitate an operator to set separation actions according to own needs to obtain a strip required by the operator; a clamping groove 7 is formed between the bottom end of the ultracentrifuge tube support 8 and the bottom of the other side of the main machine body 1, and a bayonet is formed in the position, corresponding to the clamping groove 7, of the bottom end of the ultracentrifuge tube support 8, so that the ultracentrifuge tube support 8 can be conveniently installed in the clamping groove 7; the transparent protective sheet is installed on the inner side of the photographing window 10, and the fixing glue is sleeved on the outer side of the transparent protective sheet to prevent liquid from spilling to pollute the lens of the camera 14, so that subsequent normal use is inconvenient.

The working principle is as follows: after ultracentrifugation, sample particles with different sizes and structures are layered in a density gradient medium, while the refractive indexes of different sample particles are different, so that the layered gradient liquid is irradiated by a certain light source, and the condition of each strip can be observed easily; the system provides light sources with two angles for the ultracentrifuge tube 9, the first light source is an LED lamp bead 16 at the bottom of a light-condensing and light-reflecting plate 17, the bottom of the ultracentrifuge tube 9 is illuminated through light condensation of the light-condensing and light-reflecting plate 17, and the LED lamp bead is used for imaging the gradient liquid in the ultracentrifuge tube 9 for the first time by a camera 14; the second light source is an LED lamp bead 16 arranged between the weak light plate 15 and the ultracentrifuge tube support 8, the LED lamp bead 16 illuminates the front side of the ultracentrifuge tube 9, after the light source of the LED lamp bead 16 passes through the weak light plate 15, the light becomes very uniform, and the camera 14 is just opposite to the weak light plate 15, so that the weak light plate 15 can obtain a good backlight effect picture; the backlight effect can well analyze the difference among the strips, and can be synthesized with an effect graph obtained under a bottom light source to obtain a picture with better resolution to be displayed on the touch display screen 13; a camera 14 in the main body 1 distinguishes the layered sample in the ultracentrifuge tube 9 through a photographing window 10 to perform twice imaging under two light sources, and then adjusts and combines the contrast of the pictures, and displays the pictures on a touch display screen 13; an operator can manually select which strips or which part of gradient liquid is collected on the touch display screen 13, or how many parts of gradient liquid in the whole ultracentrifuge tube 9 are collected, and the operator can judge whether one layered strip falls into two sections of pre-collected parts by dividing the pre-collected parts of the whole gradient liquid, so that the setting of the collected parts of the gradient liquid in the whole ultracentrifuge tube 9 is optimized; the bell mouth 11 moves from the upper part to the lower part of the ultracentrifuge tube 9 under the drive of the electric push rod, slowly presses gradient liquid into the fraction collecting sample tube 2, and then passes through an ultraviolet detector flow cell 18 to obtain ultraviolet absorption of a flowing sample and draw an ultraviolet absorption spectrum in a touch display screen 13; the sample can flow to the centrifuging tube in centrifuge tube rack 4 through fraction collection sample tube 2 on electronic pipettor arm 3 afterwards, and the operator can obtain the centrifuging tube that contains oneself sample through the corresponding tube number in the ultraviolet absorption map that sets up or software drawing on touch display 13 earlier stage.

The above description is only a preferred embodiment of the present invention, and should not be taken as limiting the invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. The utility model provides an intelligence gradient liquid separation system, a serial communication port, including host computer body (1), electronic transfer arm (3) are installed to host computer body (1) one side lower part, electronic transfer arm (3) front side runs through top bottom both ends and installs fraction collection sample cell (2), host computer body (1) lower part is located electronic transfer arm (3) lower position department and installs centrifuge tube rack bracket (5), centrifuge tube rack (4) are installed on centrifuge tube rack bracket (5) top, overspeed centrifuge tube support (8) are installed to host computer body (1) other side bottom, overspeed centrifuge tube support (8) inside runs through the top mid-mounting and has overspeed centrifuge tube (9), host computer body (1) opposite side lower part has seted up window of shooing (10) corresponding to overspeed centrifuge tube (9) position department, camera (14) are installed to window of shooing (10) rear end, host computer body (1) opposite side middle part is located and is equipped with horn mouth (11) in position department directly over overspeed centrifuge tube (9), be connected with electric putter between horn mouth (11) top and host computer body (1) opposite side middle part, fraction collection sample cell (2) are kept away from electronic move liquid arm (3) one end and are passed ultraviolet detector circulation cell (18) and are connected with horn mouth (11) inboard, overspeed centrifuge tube support (8) inboard front end is fixed with weak worn-out fur (15), overspeed centrifuge tube support (8) bottom is run through draw-in groove (7) and is installed spotlight reflector panel (17) with host computer body (1) outside, LED lamp pearl (16) are all installed with overspeed centrifuge tube support (8) clearance and spotlight reflector panel (17) bottom in weak worn-out fur (15).

2. The intelligent gradient liquid separation system as claimed in claim 1, wherein the electric pipetting arm (3) is connected with the main body (1) through a ball screw, the ball screw comprises a screw sleeve, a circulator and a screw rod, the screw sleeve is installed at the rear end of the electric pipetting arm (3), the circulator is installed inside the screw sleeve, the screw rod is installed at the lower position of one side of the main body (1), and one end of the screw rod is connected with a rotating motor through a reducer.

3. The intelligent gradient liquid separation system of claim 1, wherein the centrifuge tube rack bracket (5) comprises a fixed bracket, a movable bracket and a buckle, and the movable bracket is connected with the outside of the fixed bracket through the buckle.

4. The intelligent gradient liquid separation system of claim 3, wherein the outer side of the movable support is uniformly provided with limiting holes, and limiting rods are arranged in the limiting holes.

5. The intelligent gradient liquid separation system according to claim 1, wherein a main body front door (6) is hinged to the bottom of the front end of the main body (1), a power button (12) is installed in the middle of the front end of the main body (1), and a touch display screen (13) is embedded in the upper portion of the front end of the main body (1).

6. The intelligent gradient liquid separation system of claim 1, wherein a clamping groove (7) is arranged between the bottom end of the ultracentrifuge tube support (8) and the bottom of the other side of the main machine body (1), and a bayonet is arranged at the bottom end of the ultracentrifuge tube support (8) corresponding to the position of the clamping groove (7).

7. The intelligent gradient liquid separation system as claimed in claim 1, wherein a transparent protective sheet is installed on the inner side of the photographing window (10), and fixing glue is sleeved on the outer side of the transparent protective sheet.