CN215029024U - Micro-fluidic chip - Google Patents
Micro-fluidic chip Download PDFInfo
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- CN215029024U CN215029024U CN202121551072.7U CN202121551072U CN215029024U CN 215029024 U CN215029024 U CN 215029024U CN 202121551072 U CN202121551072 U CN 202121551072U CN 215029024 U CN215029024 U CN 215029024U
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Abstract
The utility model discloses a micro-fluidic chip, include: a chip body; the microfluid channel is concavely arranged on the chip body; the microfluidic channel has a detection region with a connection plane; a plurality of microspheres, each of the microspheres attached to the attachment plane to form a spherical spatial structure for attachment of a bioactive material within the detection zone. The utility model discloses technical scheme through the technical problem who sets up the microballon on detection area's connection plane, has improved micro-fluidic chip's detectivity and detection scope.
Description
Technical Field
The utility model relates to a micro-fluidic technical field, in particular to micro-fluidic chip.
Background
Microfluidic chips (Microfluidic chips) realize the functions of the whole chemical and biological laboratories by the precise manipulation and control of microfluids in a microchannel network, and are also called "Lab-on-a-chips". The microfluidic chip technology integrates basic operation units of sample preparation, reaction, separation, detection and the like in biological, chemical and medical analysis processes into a micron-scale chip, and automatically completes the whole analysis process. Due to its great potential in the fields of biology, chemistry, medicine and the like, the method has been developed into a new research field with alternative disciplines of biology, chemistry, medicine, fluid, electronics, materials, machinery and the like. The Micro total analysis system (μ TAS) is an in vitro analysis system integrating sample processing, manipulation, reaction, separation and detection, and having the characteristics of trace, high efficiency, rapidness, high flux, miniaturization, integration and automation, and meanwhile, the Micro total analysis system has significant scale effects, such as laminar flow effect, rapid mass and heat transfer effect and the like, so that the Micro total analysis system has unique advantages different from a macroscopic system.
In the related technology, the detection area of the microfluidic chip for reaction is a planar structure, so that the reaction area of the bioactive material (antigen/antibody) in the plane is very limited, and the detection sensitivity and the detection range of the microfluidic chip are greatly limited. Therefore, the design of a microfluidic chip for increasing the reaction area of bioactive materials (antigen/antibody) is urgent.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a micro-fluidic chip, aim at solving the little technical problem of traditional micro-fluidic chip antigen/antibody reaction area.
In order to achieve the above object, the present invention provides a micro-fluidic chip, including:
a chip body;
the microfluid channel is concavely arranged on the chip body; the microfluidic channel has a detection region with a connection plane;
a plurality of microspheres, each of the microspheres attached to the attachment plane to form a spherical spatial structure for attachment of a bioactive material within the detection zone.
Optionally, the microsphere is one or a combination of latex microsphere, polystyrene microsphere, fluorescent microsphere, magnetic microsphere or silica microsphere; and/or the presence of a gas in the gas,
the preparation material of the chip body is one or a combination of silicon material, glass quartz material, PS, PC, PCTG, PMMA or PDMS.
Optionally, the particle size of the microspheres is 50nm to 100 um.
Optionally, the microspheres are connected to the connection plane by physisorption or chemical bond.
Optionally, the spherical spatial structure is a single-layer spherical spatial structure; and/or the presence of a gas in the gas,
the microspheres are arranged in a rectangular shape.
Optionally, the microfluidic channel comprises a sample introduction channel and a sample discharge channel which are communicated, and an exhaust hole is formed in one end, far away from the sample introduction channel, of the sample discharge channel;
the detection area is arranged in the sample feeding channel.
Optionally, the detection area is provided with a plurality of, and a plurality of detection areas are arranged at intervals in the sample introduction channel.
Optionally, the width of the sample inlet channel is smaller than or equal to the width of the sample outlet channel; and/or the presence of a gas in the gas,
the sample outlet channel comprises a first sample outlet channel and a second sample outlet channel, the outflow end of the sample inlet channel is simultaneously communicated with the inflow end of the first sample outlet channel and the inflow end of the second sample outlet channel, and the first sample outlet channel and the second sample outlet channel are arranged around the sample inlet channel in a zigzag manner;
the exhaust hole is arranged at one end of the first sample outlet channel and one end of the second sample outlet channel, which are far away from the sample inlet channel.
Optionally, the microfluidic chip further comprises an upper cover, and the upper cover is covered and connected to the chip body; the upper cover is provided with a sample application port for adding a sample, and the sample application port is correspondingly communicated with the starting end of the sample injection channel.
Optionally, the connection plane is provided on a side of the microfluidic channel remote from the upper cover.
Compared with the prior art, the utility model discloses following beneficial effect has been obtained:
the utility model discloses among the technical scheme, through design spherical spatial structure in the detection area of microfluid passageway, effectively improved the reaction area of bioactive material (antigen/antibody), greatly improved micro-fluidic chip's detectivity and detection scope. Specifically, a microfluidic channel is arranged on a chip body for the circulation of a sample to be detected; and a detection region is provided within the microfluidic channel to enable detection of the concentration of the sample. Furthermore, in order to increase the reaction area of the bioactive materials (antigens/antibodies), a plurality of microspheres are arranged in the detection area; thus, compared with the original reaction area (i.e. the area of the connection plane) for bioactive materials (antigens/antibodies), the technical scheme of the utility model utilizes the space structure characteristics of the microspheres to connect a plurality of microspheres on the connection plane of the detection area, thereby forming a spherical space structure with a large amount of spherical outer surfaces which is far larger than the plane reaction area, and greatly improving the reaction area for connecting bioactive materials; meanwhile, the utility model connects the microspheres on the connection plane of the microfluidic channel to form a space structure, thereby effectively reducing the influence of steric hindrance caused by directly connecting the bioactive material on the plane structure by the traditional microfluidic chip; therefore, the detection sensitivity and the detection range of the micro-fluidic chip are effectively improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the structures shown in the drawings without creative efforts.
Fig. 1 is a schematic structural diagram of a chip body of a microfluidic chip according to an embodiment of the present invention;
fig. 2 is an enlarged view of a detection region of a microfluidic chip according to an embodiment of the present invention;
fig. 3 is a schematic structural diagram of an upper cover of a microfluidic chip according to an embodiment of the present invention.
The reference numbers illustrate:
| 100 | |
211 | |
| 200 | |
220 | |
| 300 | Microspheres | 221 | A first |
| 400 | |
222 | Second |
| 210 | |
410 | Sample application port |
The objects, features and advantages of the present invention will be further described with reference to the accompanying drawings.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
It should be noted that if directional indications (such as up, down, left, right, front, and back … …) are involved in the embodiment of the present invention, the directional indications are only used to explain the relative position relationship between the components, the motion situation, etc. in a specific posture, and if the specific posture is changed, the directional indications are changed accordingly.
In addition, if there is a description relating to "first", "second", etc. in the embodiments of the present invention, the description of "first", "second", etc. is for descriptive purposes only and is not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In addition, the technical solutions in the embodiments may be combined with each other, but it must be based on the realization of those skilled in the art, and when the technical solutions are contradictory or cannot be realized, the combination of the technical solutions should not be considered to exist, and is not within the protection scope of the present invention.
Referring to fig. 1 to 3, the present invention provides a microfluidic chip, including:
a chip body 100;
a microfluidic channel 200 recessed in the chip body 100; the microfluidic channel 200 has a detection region 211, the detection region 211 having a connection plane;
a plurality of microspheres 300, each of the microspheres 300 being attached to the attachment plane to form a spherical spatial structure for attaching bioactive materials within the detection region 211.
In this embodiment, in order to detect a sample to be detected, a microfluidic channel 200 through which the sample to be detected flows is disposed on the chip body 100, and a detection region 211 is disposed in the microfluidic channel 200.
It is understood that when the sample flows through the detection region 211, the bioactive material (antigen/antibody) attached within the detection region 211 captures the substance to be detected in the sample to achieve the detection of the sample concentration. Specifically, when the concentration of the substance to be detected in the sample is lower, the more bioactive materials (antigens/antibodies) contained in the detection region 211, the higher the probability that the bioactive materials (antigens/antibodies) capture the substance to be detected in the sample, and the more labeled substances linked by the reaction, the higher the signal intensity of the test. In the related art, if the concentration of the substance to be detected in the sample is higher but the detection region 211 contains less bioactive material (antigen/antibody), the detection region 211 is easily saturated, so that the accuracy of the sample concentration detection is not high.
In order to increase the reaction area for connecting bioactive materials (antigens/antibodies) in the detection region 211, a plurality of microspheres 300 are arranged in the detection region 211, the spatial structure characteristics of the microspheres 300 are supposed to increase the surface area for connecting bioactive materials (antigens/antibodies), and a spherical spatial structure is constructed in the detection region 211 by using a plurality of microspheres 300, so that the reaction area for connecting bioactive materials (antigens/antibodies) is increased, and the detection precision of the microfluidic chip is improved. Specifically, let a circle and a sphere with radius r, the surface areas of which are respectively: circular SRound (T-shaped)＝πr2Sphere SBall with ball-shaped section＝4πr2After the micro-fluidic chip is connected with the microspheres 300, the reaction area is increased by 4 times.
Thus, compared with the original reaction area (i.e. the area of the connection plane) for the bioactive material (antigen/antibody), the present invention connects a plurality of microspheres 300 to the connection plane of the detection region 211, forming a spherical space structure with a large amount of spherical outer surfaces far larger than the planar reaction area, and greatly increasing the reaction area for connecting the bioactive material (antigen/antibody); meanwhile, the micro-ball 300 is connected on the connection plane of the micro-fluid channel 200 to form a space structure, thereby effectively reducing the influence of steric hindrance caused by directly connecting a bioactive material (antigen/antibody) on the plane structure by the traditional micro-fluid chip; therefore, the detection sensitivity and the detection range of the micro-fluidic chip are effectively improved.
Optionally, the microspheres 300 are one or a combination of latex microspheres, polystyrene microspheres, fluorescent microspheres, magnetic microspheres, or silica microspheres. For example, but not limited to, the microfluidic is a magnetic microsphere.
Optionally, the preparation material of the chip body 100 is one or a combination of a silicon material, a glass quartz material, PS, PC, PCTG, PMMA, or PDMS. For example, but not limited to, the material of the chip body 100 is silicon material.
In one embodiment, the chip body 100 may be manufactured by injection molding, CNC processing, hot pressing, photolithography, and the like. The specific process is referred to the prior art, and is not described in detail herein.
Optionally, the particle size of the microsphere 300 is 50nm to 100 um. For example, but not limiting of, to save costs, the particle size of the microspheres 300 is 1 um.
Optionally, the microspheres 300 are connected to the connection plane by physical adsorption or chemical bond.
In this embodiment, in order to realize the connection between the microspheres 300 and the connection plane, the connection is performed by chemical bond. It should be understood that both the connection plane and the microfluidic surface are treated to provide the connection plane with a molecular structure bonded to the surface of the microsphere 300.
In other embodiments, hydrophilic and hydrophobic properties may also be utilized between the microspheres 300 and the connection plane to achieve connection by physical adsorption.
Optionally, to save raw materials, the spherical spatial structure is a single-layer spherical spatial structure. Thus, the surface area utilization rate of the microspheres 300 is effectively improved.
Optionally, the detection region 211 is rectangular, and a plurality of microspheres 300 are arranged in order at intervals to be rectangular, and cover the connection plane in the detection region 211.
Optionally, the microfluidic channel 200 includes a sample inlet channel 210 and a sample outlet channel 220 which are communicated, and an end of the sample outlet channel 220 away from the sample inlet channel 210 is provided with a vent hole;
the detection area 211 is disposed in the sample inlet channel 210.
In this embodiment, a sample introduction channel 210 is provided for detecting a sample. Meanwhile, in order to realize the recovery of the waste liquid after detection, a sample outlet channel 220 is arranged; and an exhaust hole is arranged at one end of the sample outlet channel 220 far away from the sample inlet channel 210, so that waste liquid can be conveniently discharged from the microfluidic chip.
Optionally, the detection area 211 is provided in a plurality, and the detection areas 211 are provided at intervals in the sample inlet channel 210.
In this embodiment, in order to realize high-precision measurement of the concentration of a sample to be measured, the sample introduction channel 210 is provided with a plurality of detection areas 211 arranged at intervals. It should be understood that each of the detection regions 211 has a spherical space structure formed by connecting a plurality of microspheres 300.
Optionally, to improve high precision detection and high utilization of the sample, the width of the sample inlet channel 210 is set to be less than or equal to the width of the sample outlet channel 220. So, when the discharge waste liquid, the passageway of waste liquid accessible broad flows out fast, by doing benefit to and improving whole detection efficiency.
Optionally, the sample outlet channel 220 includes a first sample outlet channel 221 and a second sample outlet channel 222, an outflow end of the sample inlet channel 210 communicates with an inflow end of the first sample outlet channel 221 and an inflow end of the second sample outlet channel 222 simultaneously, and the first sample outlet channel 221 and the second sample outlet channel 222 are both disposed around the sample inlet channel 210 in a zigzag manner;
the exhaust hole is disposed at one end of the first sample outlet channel 221 and the second sample outlet channel 222 far away from the sample inlet channel 210.
In this embodiment, for the discharge efficiency who improves the waste liquid, set up two play appearance passageways. Specifically, the two sample outlet channels are communicated and arranged around the outlet end of the sample inlet channel 210 towards two opposite sides in a zigzag manner. Thus, the ends of the first sample outlet channel 221 and the second sample outlet channel 222 provided with the exhaust holes are both disposed near the starting end (inflow end) of the sample inlet channel 210.
Optionally, the microfluidic chip further includes an upper cover 400, and the upper cover 400 is covered and connected to the chip body 100; the upper cover 400 is provided with a sample application port 410 for adding a sample, and the sample application port 410 is correspondingly communicated with the starting end of the sample feeding channel 210.
In this embodiment, an upper cover 400 is provided to form a closed environment of the microfluidic channel 200. The upper cover 400 is provided with a sample application port 410 which penetrates through the upper cover 400 and corresponds to the starting end of the sample introduction channel 210, so that a sample to be detected can be added and can enter the microfluidic channel 200 for detection.
In one embodiment, the upper cover 400 and the chip body 100 may be welded by ultrasonic welding. In other embodiments, the cover 400 and the chip may be adhered together by double-sided adhesive bonding.
Optionally, the connection plane is provided on a side of the microfluidic channel 200 remote from the upper cover 400.
In this embodiment, in order to save the material of the microspheres 300, the microspheres 300 are connected to the bottom of the microfluidic channel 200 (i.e., the side away from the upper cover 400), so that the amount of the microspheres 300 can be reduced, and the detection accuracy can be ensured.
The above is only the optional embodiment of the present invention, and not therefore the limit of the patent scope of the present invention, all of which are in the concept of the present invention, the equivalent structure transformation of the content of the specification and the drawings is utilized, or the direct/indirect application is included in other related technical fields in the patent protection scope of the present invention.
Claims (10)
1. A microfluidic chip, comprising:
a chip body;
the microfluid channel is concavely arranged on the chip body; the microfluidic channel has a detection region with a connection plane;
a plurality of microspheres, each of the microspheres attached to the attachment plane to form a spherical spatial structure for attachment of a bioactive material within the detection zone.
2. The microfluidic chip according to claim 1, wherein the microspheres are one or a combination of latex microspheres, polystyrene microspheres, fluorescent microspheres, magnetic microspheres, or silica microspheres; and/or the presence of a gas in the gas,
the preparation material of the chip body is one or a combination of silicon material, glass quartz material, PS, PC, PCTG, PMMA or PDMS.
3. The microfluidic chip according to claim 2, wherein the microspheres have a particle size of 50nm to 100 um.
4. The microfluidic chip of claim 1, wherein said microspheres are attached to said connection plane by physical adsorption or chemical bonding.
5. The microfluidic chip of claim 4, wherein the spherical spatial structure is a single-layer spherical spatial structure; and/or the presence of a gas in the gas,
the microspheres are arranged in a rectangular shape.
6. The microfluidic chip according to claim 1, wherein the microfluidic channel comprises a sample inlet channel and a sample outlet channel, which are communicated, and an end of the sample outlet channel away from the sample inlet channel is provided with a vent hole;
the detection area is arranged in the sample feeding channel.
7. The microfluidic chip according to claim 6, wherein a plurality of the detection regions are provided, and a plurality of the detection regions are provided at intervals in the sample channel.
8. The microfluidic chip of claim 7, wherein the width of the sample channel is less than or equal to the width of the sample channel; and/or the presence of a gas in the gas,
the sample outlet channel comprises a first sample outlet channel and a second sample outlet channel, the outflow end of the sample inlet channel is simultaneously communicated with the inflow end of the first sample outlet channel and the inflow end of the second sample outlet channel, and the first sample outlet channel and the second sample outlet channel are arranged around the sample inlet channel in a zigzag manner;
the exhaust hole is arranged at one end of the first sample outlet channel and one end of the second sample outlet channel, which are far away from the sample inlet channel.
9. The microfluidic chip of claim 8, further comprising an upper cap, wherein the upper cap is coupled to the chip body; the upper cover is provided with a sample application port for adding a sample, and the sample application port is correspondingly communicated with the starting end of the sample injection channel.
10. The microfluidic chip of claim 9, wherein said connection plane is disposed on a side of said microfluidic channel remote from said cover.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121551072.7U CN215029024U (en) | 2021-07-08 | 2021-07-08 | Micro-fluidic chip |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121551072.7U CN215029024U (en) | 2021-07-08 | 2021-07-08 | Micro-fluidic chip |
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| CN215029024U true CN215029024U (en) | 2021-12-07 |
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| CN202121551072.7U Active CN215029024U (en) | 2021-07-08 | 2021-07-08 | Micro-fluidic chip |
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- 2021-07-08 CN CN202121551072.7U patent/CN215029024U/en active Active
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